FastQCFastQC Report
Sat 31 Dec 2022
SRR5849001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34420
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC9332.7106333527019175No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT8952.600232423009878No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1670.4851830331202789No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1310.3805926786751888No Hit
TTTCTTGGGATATCCAAGGAATCATTGCCAAATTCAGTGTCATGAAGTTTTC1080.3137710633352702No Hit
GCTAAAGGGAGAATATATGTTGGACCTCTGAAAAGTTGTAAGGGGCCAATTC990.28762347472399763No Hit
AGAGGCGGGGAGAAGAATATTAGAGAAATACTGACCAAAGGTCAAAGATTTC890.25857059848925046No Hit
AACTCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA880.2556653108657757No Hit
ATCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA860.24985473561882626No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCTTATAC820.23823358512492734No Hit
GACAAGGGATGCCACTGCACTTCAACTTGGGCAACAGAGCAAGACCCTGTCT820.23823358512492734No Hit
GTCTTAGGGGATGCAAGGATGTAGTTTTGTACTGAAAGAATAAACACTGACT810.23532829750145265No Hit
GTTCTGGGGTCAGAGGGCCATGAGGTTGCATTTTTTAAATTGAGGTAAAATT780.22661243463102848No Hit
TGGAAGGGAGATGGGTTAGATAAAGATGAATATACATTTTCACCAGGCAAAT770.22370714700755373No Hit
AATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA750.21789657176060429No Hit
AGCACAGGGATAATTTGTGATCCTGGGTTTTCCTTCCTTAAAAATACTTCTT690.20046484601975598No Hit
ATTCTGGGGATTCATCTCTGTCTAGAGTATTTCATGTGTGTATGTGTTGCTT680.19755955839628123No Hit
TCAGCGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAG670.1946542707728065No Hit
CATTTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC670.1946542707728065No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA660.19174898314933178No Hit
GCTTTAGGGAGATGGACAAGGAAGCAATTTGAGTAATTTTGTGAAAATTTCA660.19174898314933178No Hit
ATCGGGGTATCTACGCCTGGGTAGTTCAGTACTCCAAATTATTGGGGACCTT650.18884369552585706No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC630.1830331202789076No Hit
TCCTGGGGGAAGGCTGTGATTATGTTTGATATTCACCTGGATTTTAATACAA630.1830331202789076No Hit
TAGCGAGGGGAGACAGTTTTATCATCCACATTGCTGACCTGGAAGATGCATC630.1830331202789076No Hit
GTAACAGGGGCTGATAAATGTATCAGAAACTCTTTCATCATTTTCTGTCCCC620.1801278326554329No Hit
TGCAGAGGGAAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGG600.17431725740848344No Hit
TTTAACGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT600.17431725740848344No Hit
TTGAAGCGGGAGGGTGTTCTTTTTTATGAAGAAAAATTTGAAAATGATAAAA580.16850668216153397No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATACTGTCTCTTATACAC570.16560139453805925No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT570.16560139453805925No Hit
TAGATGGGATGTTTTGTTTTGTCTGTTTTTTGCTATTTGATTTCTGTACCTG560.16269610691458455No Hit
CATCATGGGATTCTGTAAAAACTCTTTATAAAAAGTTACTATTCGTATCTGT550.15979081929110983No Hit
GTAACTGGGACTACAGCGGAGGAGATGCCCATGAATGTGGCTGACCTTATTT550.15979081929110983No Hit
CAGGCAGGGAGTCACTCTGGTTTTGTAATGATTTGCCTTTTAGGCCTCAGGC530.15398024404416039No Hit
ACAGTTGGGCGCTTGTGGTCCTCCAACAACTAAATCTCATCCCCCATGAGGT530.15398024404416039No Hit
TCAACCGGGAATTATAGCCTTTTAAGAAAATAATCAAAGAAGTAAGTTTCAT530.15398024404416039No Hit
TAGATGGGAATCATAATAATAATTTAAAAGAGTGATGATACCAAGTGTTGGT530.15398024404416039No Hit
CTTATGGGGATATTTCCCAATCATTGTCTTCATTCCACTACAAAGTCACCTC530.15398024404416039No Hit
AATTGTGGGATGGGGACCTGGAACCTGCCCCTTATCCAACCAAGTAATTAGG520.15107495642068564No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCT520.15107495642068564No Hit
CGGACCGGGGCTTTATGACTGGGTGTGTGTTGTGTGTATGTAATACATAATG520.15107495642068564No Hit
GATGCTGGGATGGTGCCTGGCCAAACGTTTTTATTACAAATATTACATTAAA520.15107495642068564No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATAT510.1481696687972109No Hit
ACTACGGGAGTGTGGGAAAAGTGTTTCCATTCTGGGAAAAGCCCAAACCGAA510.1481696687972109No Hit
ATTCCGGGGAGACTCAGGCTTACCTTGCACAAAAATAAATTCCTTTTCATAT500.1452643811737362No Hit
CTCACATGGGGAAAAGATAAATTGACAAACCATTAGCCAGACTAAGAAAAAA480.13945380592678677No Hit
TTAGCAGGGATGTCAAGTGCAGTTGTGAGGACTGTGTGGGTGAACGAAAATG460.1336432306798373No Hit
ACAAGAGGGCGTTTCTTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCA460.1336432306798373No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATAACTCGTATGC450.13073794305636258No Hit
ATTGTTGGGATACATGTGCAAGGGAGACAAAAGAACTCAACAGAATTTTACT450.13073794305636258No Hit
GCATTTGGGGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAA450.13073794305636258No Hit
CTGTTTGGGAAGACATTTTTCCTGATATGTAGAGCAGTCTGTTGGCAAAAAT440.12783265543288785No Hit
GTGCGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTGA430.12492736780941313No Hit
CAACTGGGGGAAATGGGAAATAGATAAAATTCATTTTAAAAAACAAGCCTAC430.12492736780941313No Hit
GTACCTGGGAAAAGAAAATTGAGAGAAAGGACTACAGAGCCCCGAATTAATA430.12492736780941313No Hit
ACTTAGGGTCACCTTAAAAAAACAGAATGAGTCATAAGGAGTCAGACATGCC430.12492736780941313No Hit
AGTAATGGGACTGCGGGGATAGTTGATGTAACATTGTAGAAGCAGCATAGGA430.12492736780941313No Hit
CGTTCTGGGAGAGTATTGCAGGCAATAATACGACTAAAATTCTAATACTTTT420.12202208018593842No Hit
TTATGGGGACCCCTAAAATTTTAAGAACTGTACTTTATAAAAATGTGCAATT420.12202208018593842No Hit
TTTCTAGGGAGAACTAGTAGTACTAGTAGTACTGTCTCTAGCTCTTCATACA420.12202208018593842No Hit
GTATGTGGGATTTTTGTCTATGGTAGTTATTTCACAAAGCCAAGCGAGTTAC420.12202208018593842No Hit
AACTTAGGGATTCTGCACATGTATCCCAGAACTTAAAGTAAAATAAAATAAT420.12202208018593842No Hit
TGTTTAGGGAGCATGATTTTTTTGCACATGTAGAAATTTTTTAAAAGAAAGA410.11911679256246367No Hit
AGCATCGGGAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG410.11911679256246367No Hit
CACTCAGGGACCCTGGGTACTGAACAAAAAAACCCAAATTACACTCCTTATT410.11911679256246367No Hit
CTGAGCGGGAGGTAGCTGGGACCTAGGCTATCTTACCATGAAGGTTGTTTTG410.11911679256246367No Hit
TATAGCGGGCTTTGTGATCTCATAGCTTGCTACGCTTTTATATCTTATGGCT400.11621150493898895No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATG400.11621150493898895No Hit
CATTTCATTTCTTAGGCATTTTCTCTCTTATAAAGTTTCCTGGATTTATAAA400.11621150493898895No Hit
GGATATGGGACTACGACGTCGCGAAGCAGCTGCTGGCTGCAGGTGCGGAGGT390.11330621731551424No Hit
ATACATGGGGGTTGAGCTGATGTCAATTGGTTCTTGTCTTGAGTCGACTCAA390.11330621731551424No Hit
GTTAAAGGGATGTCTGAATTAGAAAGCCAGAGGTGAGGTCTGGGTTGGAGAT390.11330621731551424No Hit
ACGAGGGAATTCTGAAAATAAGAACCTGTTAATAGGTTGGAAAGCTGTGTAC380.11040092969203952No Hit
TGCTAAGGGCGATCTTATCCCGGGAGAATGCACAGAGCCAATGCATTTTTGG380.11040092969203952No Hit
ATGCATGGGAGTGGGAGATTTCCACACTCCAATGACAGTATTAGACAGATGA380.11040092969203952No Hit
TCCAAAGGGAGAACCCTTGATGAGACTATTTTTAAACATACTAGTCTGCTGA370.1074956420685648No Hit
GGGCAGGGGATGAGATTGATTTGTTGTGAAGTTATTGGCTATAATGTCTTTG370.1074956420685648No Hit
TATGCTGGGAGGCATCACCCCGCTAAATCCCCTAGAAGTCCCACTCCTAAAC370.1074956420685648No Hit
CATCATGGGGATAAAAGGGTACATCCATCAAGAAAATATCACCGTTATCAAC370.1074956420685648No Hit
GGCCAGGGGATGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGT370.1074956420685648No Hit
CCAAAAGGGAAGACTGATGCTTAATACACAGTCTGTTCTCCTGTGTCTAGGT370.1074956420685648No Hit
TGCTACGGGCAAATCACTCAGCAGCTGTTTTTGTTTGTTTGTTTGTTTGTTT360.10459035444509006No Hit
GACCAGGGGAATCCCTTAAGCATTTGATGAAACACTCTTTAGTGCTATATGC360.10459035444509006No Hit
ACTATGGGGGGCTCTGTGGCTGTGCCTTCACCCTCGCTGATGTCCTCCTGGG360.10459035444509006No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATACTGTCTCTTA360.10459035444509006No Hit
GTCAAAGGGGCCTGTGTTGTTGCTATGTGTATTTGAAAGTAATATCTGCATT350.10168506682161534No Hit
ATTGTAGGGACTTCCTGTTTTGTCACAAGGAACATTAAAAAGGCGTGAGCCA350.10168506682161534No Hit
CTATAGGGGAGAGCCGCCTTGCTCCGAACCTTCAGCTTTTTGGGCTTTGAGA350.10168506682161534No Hit
AATTAAGGGGCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTC350.10168506682161534No Hit
AGCACCGGGAGTTCTGGCCATTCTGAGCCTGCTTTTGTGATTGCTCATCCAT350.10168506682161534No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)