FastQCFastQC Report
Sat 31 Dec 2022
SRR5849000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5849000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34828
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT9822.8195704605489835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC9412.7018490869415412No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1820.5225680486964511No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1310.3761341449408522No Hit
AGAGGCGGGGAGAAGAATATTAGAGAAATACTGACCAAAGGTCAAAGATTTC1070.3072240725852762No Hit
GCTAAAGGGAGAATATATGTTGGACCTCTGAAAAGTTGTAAGGGGCCAATTC1050.3014815665556449No Hit
TTTCTTGGGATATCCAAGGAATCATTGCCAAATTCAGTGTCATGAAGTTTTC910.26128402434822556No Hit
GTTCTGGGGTCAGAGGGCCATGAGGTTGCATTTTTTAAATTGAGGTAAAATT880.25267026530377856No Hit
GACAAGGGATGCCACTGCACTTCAACTTGGGCAACAGAGCAAGACCCTGTCT860.24692775927414723No Hit
TAGATGGGAATCATAATAATAATTTAAAAGAGTGATGATACCAAGTGTTGGT780.2239577351556219No Hit
GTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCC770.22108648214080626No Hit
GTAACTGGGACTACAGCGGAGGAGATGCCCATGAATGTGGCTGACCTTATTT750.21534397611117492No Hit
TAGCGAGGGGAGACAGTTTTATCATCCACATTGCTGACCTGGAAGATGCATC740.21247272309635923No Hit
AACTCTGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA710.20385896405191226No Hit
GTAACAGGGGCTGATAAATGTATCAGAAACTCTTTCATCATTTTCTGTCCCC710.20385896405191226No Hit
ATTCTGGGGATTCATCTCTGTCTAGAGTATTTCATGTGTGTATGTGTTGCTT700.20098771103709662No Hit
GTCTTAGGGGATGCAAGGATGTAGTTTTGTACTGAAAGAATAAACACTGACT700.20098771103709662No Hit
AGCACAGGGATAATTTGTGATCCTGGGTTTTCCTTCCTTAAAAATACTTCTT700.20098771103709662No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCCTGTCTCTTATAC690.19811645802228092No Hit
TCAACCGGGAATTATAGCCTTTTAAGAAAATAATCAAAGAAGTAAGTTTCAT680.19524520500746526No Hit
TTTCTAGGGAGAACTAGTAGTACTAGTAGTACTGTCTCTAGCTCTTCATACA670.1923739519926496No Hit
ATCGGGGTATCTACGCCTGGGTAGTTCAGTACTCCAAATTATTGGGGACCTT660.18950269897783392No Hit
CAGGCAGGGAGTCACTCTGGTTTTGTAATGATTTGCCTTTTAGGCCTCAGGC650.18663144596301826No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA650.18663144596301826No Hit
GATGCTGGGATGGTGCCTGGCCAAACGTTTTTATTACAAATATTACATTAAA650.18663144596301826No Hit
ATCTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA640.1837601929482026No Hit
ATTGTTGGGATACATGTGCAAGGGAGACAAAAGAACTCAACAGAATTTTACT640.1837601929482026No Hit
ATGCATGGGAGTGGGAGATTTCCACACTCCAATGACAGTATTAGACAGATGA640.1837601929482026No Hit
TTGAAGCGGGAGGGTGTTCTTTTTTATGAAGAAAAATTTGAAAATGATAAAA630.18088893993338692No Hit
CGGACCGGGGCTTTATGACTGGGTGTGTGTTGTGTGTATGTAATACATAATG620.17801768691857126No Hit
ACTACGGGAGTGTGGGAAAAGTGTTTCCATTCTGGGAAAAGCCCAAACCGAA610.1751464339037556No Hit
CATCATGGGATTCTGTAAAAACTCTTTATAAAAAGTTACTATTCGTATCTGT600.17227518088893992No Hit
CTTATGGGGATATTTCCCAATCATTGTCTTCATTCCACTACAAAGTCACCTC600.17227518088893992No Hit
GCTTTAGGGAGATGGACAAGGAAGCAATTTGAGTAATTTTGTGAAAATTTCA600.17227518088893992No Hit
ACGAGGGAATTCTGAAAATAAGAACCTGTTAATAGGTTGGAAAGCTGTGTAC590.16940392787412425No Hit
TGGAAGGGAGATGGGTTAGATAAAGATGAATATACATTTTCACCAGGCAAAT550.15791891581486162No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT550.15791891581486162No Hit
TTTAACGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT540.15504766280004595No Hit
CTGAGCGGGAGGTAGCTGGGACCTAGGCTATCTTACCATGAAGGTTGTTTTG540.15504766280004595No Hit
AATCCAGGTCGGTTTCTATCTACTTCAAATTCCTCCCTGTACGAAAGGACAA530.15217640978523028No Hit
CATTTAGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC530.15217640978523028No Hit
ACAAGAGGGCGTTTCTTTGAGGAGTGTGACCCCAACAAGGATAAGCACATCA530.15217640978523028No Hit
GTATGTGGGATTTTTGTCTATGGTAGTTATTTCACAAAGCCAAGCGAGTTAC520.14930515677041462No Hit
GTTAAAGGGATGTCTGAATTAGAAAGCCAGAGGTGAGGTCTGGGTTGGAGAT520.14930515677041462No Hit
GGGCAGGGGATGAGATTGATTTGTTGTGAAGTTATTGGCTATAATGTCTTTG510.14643390375559895No Hit
CTCACATGGGGAAAAGATAAATTGACAAACCATTAGCCAGACTAAGAAAAAA500.14356265074078328No Hit
AATTGTGGGATGGGGACCTGGAACCTGCCCCTTATCCAACCAAGTAATTAGG480.13782014471115195No Hit
ACAGTTGGGCGCTTGTGGTCCTCCAACAACTAAATCTCATCCCCCATGAGGT470.13494889169633628No Hit
GGCCAGGGGATGAGAATACAGGAAATGGCTCTTGTTTATTTTTTTGCTGTGT470.13494889169633628No Hit
AGTAATGGGACTGCGGGGATAGTTGATGTAACATTGTAGAAGCAGCATAGGA470.13494889169633628No Hit
AACACTGGGAATTGGGAGTGGTAGGATGAAACAATTTGGAGAAGATAGAAGT460.13207763868152061No Hit
TTAGCAGGGATGTCAAGTGCAGTTGTGAGGACTGTGTGGGTGAACGAAAATG460.13207763868152061No Hit
CATCATGGGGATAAAAGGGTACATCCATCAAGAAAATATCACCGTTATCAAC460.13207763868152061No Hit
TCAGCGGGAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACTTAAACAG450.12920638566670495No Hit
GCTTATGGGAGATGAGGAAATTGTGCAGTTCTGGTACATAAAGATGAGTAAT440.12633513265188928No Hit
ATTCCGGGGAGACTCAGGCTTACCTTGCACAAAAATAAATTCCTTTTCATAT440.12633513265188928No Hit
ACTTAGGGTCACCTTAAAAAAACAGAATGAGTCATAAGGAGTCAGACATGCC440.12633513265188928No Hit
TGCAGAGGGAAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACGCAGG430.12346387963707361No Hit
CAACTGGGGGAAATGGGAAATAGATAAAATTCATTTTAAAAAACAAGCCTAC430.12346387963707361No Hit
TGCTATGGGTGGCCGGGCGCAGTGGCTCATGCCTGTAGTCCCAGCGCTTTGG430.12346387963707361No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATAT420.12059262662225795No Hit
CACTCAGGGACCCTGGGTACTGAACAAAAAAACCCAAATTACACTCCTTATT420.12059262662225795No Hit
ATTTATGGGCTCTCTCGCGCGCGGTGTGGTGGCAGCAGGCGCAGCCCAGCCT420.12059262662225795No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATACTGTCTCTTATACAC410.11772137360744228No Hit
ATTGTAGGGACTTCCTGTTTTGTCACAAGGAACATTAAAAAGGCGTGAGCCA410.11772137360744228No Hit
AGCATCGGGAACGCAGACGAAAATGGAAAGATTAATTGGGAGTGGTAGGATG410.11772137360744228No Hit
AGCACCGGGAGTTCTGGCCATTCTGAGCCTGCTTTTGTGATTGCTCATCCAT410.11772137360744228No Hit
TGTTTAGGGAGCATGATTTTTTTGCACATGTAGAAATTTTTTAAAAGAAAGA400.11485012059262661No Hit
TCCTGGGGGAAGGCTGTGATTATGTTTGATATTCACCTGGATTTTAATACAA400.11485012059262661No Hit
TAGATGGGATGTTTTGTTTTGTCTGTTTTTTGCTATTTGATTTCTGTACCTG390.11197886757781095No Hit
TCCAAAGGGAGAACCCTTGATGAGACTATTTTTAAACATACTAGTCTGCTGA380.10910761456299528No Hit
GTTACAGGGAGAGCTTAAAAGTTTATAAGCATTAAAAACACATACATGTATT380.10910761456299528No Hit
CATTTCATTTCTTAGGCATTTTCTCTCTTATAAAGTTTCCTGGATTTATAAA380.10910761456299528No Hit
CGTTCTGGGAGAGTATTGCAGGCAATAATACGACTAAAATTCTAATACTTTT370.10623636154817961No Hit
TGCTAAGGGCGATCTTATCCCGGGAGAATGCACAGAGCCAATGCATTTTTGG370.10623636154817961No Hit
GCATTTGGGGTGGCGCGGCTTCTGTGGGCCGGAACCTTAAAGATAGCCGCAA370.10623636154817961No Hit
TATAGCGGGCTTTGTGATCTCATAGCTTGCTACGCTTTTATATCTTATGGCT370.10623636154817961No Hit
GTTTTTGGGTGGAGTAGGAAAACATAAGCTTGAAGAAGGAAGCTTTAAAGAA360.10336510853336398No Hit
CTGTTTGGGAAGACATTTTTCCTGATATGTAGAGCAGTCTGTTGGCAAAAAT360.10336510853336398No Hit
GTCAAAGGGGCCTGTGTTGTTGCTATGTGTATTTGAAAGTAATATCTGCATT350.10049385551854831No Hit
GTGGCAGGGAGAGCCTTGAGTAAACATCTTTTCAGGATTTTGTGTGTTGAAA350.10049385551854831No Hit
GTGCTCGGGAATAATTGTCCATGAGGAGAAAAAAAGCATGTGTCATGCATGA350.10049385551854831No Hit
AACTACAGGAGAAGATAAGCGAATTCCCCGAGTGTCAGGTTGGTGGCGTGTT350.10049385551854831No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)