FastQCFastQC Report
Sat 31 Dec 2022
SRR5848996.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848996.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69866
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC9731.3926659605530587No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT8441.208026794148799No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1790.2562047347780036No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1650.23616637563335527No Hit
CTTCCTGGGGGACTTTCGTGGGATGAGGGAAAGCTTCCTAGAGGGCGCTACC1640.2347350642658804No Hit
CACACAGGGGAACATGGTGAGCTGGGTGGAAAACAATGAGTTAGGTCTTGCA1430.2046775255489079No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1330.19036441187415912No Hit
AAACTTGGGAGAAATCCCCCATGCTTCCCTCTGCTGGGGGCAGATGATATCA1190.1703260527295108No Hit
GTAGCAGGGACAGTCTCACAACATTTAGTCTTTTTAGAATAATAAAATCATA1070.1531503163198122No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1030.1474250708499127No Hit
TCTAAAGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1000.14313113674748804No Hit
CAACTCGGGAAATATAAAAAATTTGTATAAAGCACTTTCCTTAGAAAAAGTG990.1416998253800132No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT980.1402685140125383No Hit
TGCTGGGTTAGAATGAAGACTTTAAATATCAATACTTTTTCTGAATGACAAG920.131680645807689No Hit
ATTGCTGGGGGGGTGGCAGCTAGAGCCCAGGCCATAGGTAGCGTAGGCTGGG900.12881802307273924No Hit
TGTCCTCGGGAGGCTGCAGTTTAAATATCACTGTCTCCCTCAGGGCTTCTAT870.12452408897031461No Hit
TACCAAGGGGGAAGAATTATTTATGTAGGTTTTTATAAAAATTATTTATGCA860.12309277760283972No Hit
ACCATGGGTCAGCAGATTTGCCAGCTGTGAGATAACTAAGGCACATTAAACC820.1173675321329402No Hit
CGTCAGGGGTAGTGTTGAGAATTAACGGCTTTCCCAGGATTCCAGTTTGCTT810.11593622076546532No Hit
TTCGTTGGGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGGATCT800.11450490939799043No Hit
GGTGCTGGGTCAAATGGTATTTCTGGCTCTAGATCTTGAGGAATCACCACAC790.11307359803051556No Hit
AGACATGGGAACTGCCTGATGGGACCGTTGCTCCATCTGAAGCATTATCTTC780.11164228666304067No Hit
ACAAATTGGGCAGGAACCATGCAGGATGGCAGCTCTCCATGTGTCTTAGGAT780.11164228666304067No Hit
ATAGATGGGGAAGAAGCAACCAAGAGAGTGCCCAAACTGAAAAAGCTGGATG740.10591704119314116No Hit
AAAATGGGGGTTACTTTTTATTTATTATTTCTTTAAAGATAAGCATTAATGT720.1030544184581914No Hit
CATTCAGGGACTGGTGAGCATTTGGTGAATTCTACTTTTTTAAACTGGCCAA710.10162310709071652No Hit
TCTAATGGGACCCTGATTTCCTCTTGAAATGATCTATATGTGAATGAAAATT700.10019179572324163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)