FastQCFastQC Report
Sat 31 Dec 2022
SRR5848995.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848995.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69691
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC10241.4693432437473992No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT7541.0819187556499406No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1680.2410641259273077No Hit
CTTCCTGGGGGACTTTCGTGGGATGAGGGAAAGCTTCCTAGAGGGCGCTACC1550.2224103542781708No Hit
CACACAGGGGAACATGGTGAGCTGGGTGGAAAACAATGAGTTAGGTCTTGCA1540.2209754487666987No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1500.21523582672081043No Hit
AAACTTGGGAGAAATCCCCCATGCTTCCCTCTGCTGGGGGCAGATGATATCA1230.17649337791106456No Hit
TCTAAAGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1170.16788394484223212No Hit
CAACTCGGGAAATATAAAAAATTTGTATAAAGCACTTTCCTTAGAAAAAGTG1130.16214432279634386No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1120.1607094172848718No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1040.14923017319309523No Hit
ACCATGGGTCAGCAGATTTGCCAGCTGTGAGATAACTAAGGCACATTAAACC920.1320113070554304No Hit
CGTCAGGGGTAGTGTTGAGAATTAACGGCTTTCCCAGGATTCCAGTTTGCTT880.1262716850095421No Hit
TGTCTTGGGATAAATGTCTTTTTAAAAGTACATTAAAAATTATGGGCATCTT860.12340187398659798No Hit
CATGATGGGCTAGCCAGGGGGGGACCTAAGATGGCGGCCTCCAGGGGGCTGG840.12053206296365385No Hit
TTCGTTGGGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGGATCT830.11909715745218177No Hit
ATTGCTGGGGGGGTGGCAGCTAGAGCCCAGGCCATAGGTAGCGTAGGCTGGG830.11909715745218177No Hit
TGTCCTCGGGAGGCTGCAGTTTAAATATCACTGTCTCCCTCAGGGCTTCTAT800.11479244091776555No Hit
GTAGCAGGGACAGTCTCACAACATTTAGTCTTTTTAGAATAATAAAATCATA790.1133575354062935No Hit
TCTAATGGGACCCTGATTTCCTCTTGAAATGATCTATATGTGAATGAAAATT790.1133575354062935No Hit
TGCTGGGTTAGAATGAAGACTTTAAATATCAATACTTTTTCTGAATGACAAG790.1133575354062935No Hit
GCATTTGGGAATTTCTATAGGAAAGAGGTCCTATACAGTTTCCATGCTGTAA790.1133575354062935No Hit
AAAATGGGGGTTACTTTTTATTTATTATTTCTTTAAAGATAAGCATTAATGT750.10761791336040522No Hit
GGTGCTGGGTCAAATGGTATTTCTGGCTCTAGATCTTGAGGAATCACCACAC720.10331319682598901No Hit
GTTATGGGGCCTTCTCTGTTTCTCTCTGTCTCCCTGTCTCTCTCTCTCTCTG720.10331319682598901No Hit
AGACATGGGAACTGCCTGATGGGACCGTTGCTCCATCTGAAGCATTATCTTC710.10187829131451695No Hit
TACCAAGGGGGAAGAATTATTTATGTAGGTTTTTATAAAAATTATTTATGCA710.10187829131451695No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT710.10187829131451695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)