FastQCFastQC Report
Sat 31 Dec 2022
SRR5848994.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848994.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69219
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC9401.3580086392464497No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT8161.1788670740692584No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1750.25282075730651987No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1690.24415261705601063No Hit
CTTCCTGGGGGACTTTCGTGGGATGAGGGAAAGCTTCCTAGAGGGCGCTACC1530.22103757638798596No Hit
CACACAGGGGAACATGGTGAGCTGGGTGGAAAACAATGAGTTAGGTCTTGCA1400.20225660584521593No Hit
AAACTTGGGAGAAATCCCCCATGCTTCCCTCTGCTGGGGGCAGATGATATCA1270.18347563530244584No Hit
CGGCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT1180.17047342492668197No Hit
TCTAAAGGGAATCTACACTAAGACCAAATGGACCTAATAGATATTTACAAAA1110.16036059463442118No Hit
CAACTCGGGAAATATAAAAAATTTGTATAAAGCACTTTCCTTAGAAAAAGTG1110.16036059463442118No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA990.1430243141334027No Hit
TGTCCTCGGGAGGCTGCAGTTTAAATATCACTGTCTCCCTCAGGGCTTCTAT960.13869024400814806No Hit
CTCTGAGCGGGTACATCTTAAACTTGAACTTGATTAGATTTTTGTCGCCGTA960.13869024400814806No Hit
GTAGCAGGGACAGTCTCACAACATTTAGTCTTTTTAGAATAATAAAATCATA890.12857741371588724No Hit
CGTCAGGGGTAGTGTTGAGAATTAACGGCTTTCCCAGGATTCCAGTTTGCTT880.1271327236741357No Hit
ACCATGGGTCAGCAGATTTGCCAGCTGTGAGATAACTAAGGCACATTAAACC870.12568803363238418No Hit
CATGATGGGCTAGCCAGGGGGGGACCTAAGATGGCGGCCTCCAGGGGGCTGG870.12568803363238418No Hit
TTCGTTGGGGAACTTGAGTTATTAAAATCATAGGAATAAAATTATGGGATCT850.1227986535488811No Hit
ACAAATTGGGCAGGAACCATGCAGGATGGCAGCTCTCCATGTGTCTTAGGAT850.1227986535488811No Hit
ATCCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGT810.11701989338187492No Hit
TACCAAGGGGGAAGAATTATTTATGTAGGTTTTTATAAAAATTATTTATGCA770.11124113321486874No Hit
GGTGCTGGGTCAAATGGTATTTCTGGCTCTAGATCTTGAGGAATCACCACAC770.11124113321486874No Hit
CTGCAAGGGTAATCTTCTTATAATCTGCCATTTCTGCCAAATCTGATTACAT740.10690706308961412No Hit
TGAATGGGGATTCTGAATTCTATCAGAACAATTCAACCAAAATCCAACTTCA730.10546237304786259No Hit
ATAGATGGGGAAGAAGCAACCAAGAGAGTGCCCAAACTGAAAAAGCTGGATG720.10401768300611104No Hit
AGACATGGGAACTGCCTGATGGGACCGTTGCTCCATCTGAAGCATTATCTTC720.10401768300611104No Hit
AAAATGGGGGTTACTTTTTATTTATTATTTCTTTAAAGATAAGCATTAATGT710.1025729929643595No Hit
TAATGAGGGCGAGAGGGTCCTGGGTCCTGTTCCTTTGCCTTTCAAAAAGACT700.10112830292260797No Hit
TGCTGGGTTAGAATGAAGACTTTAAATATCAATACTTTTTCTGAATGACAAG700.10112830292260797No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)