FastQCFastQC Report
Sat 31 Dec 2022
SRR5848987.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848987.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90478
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC10911.2058179889033798No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT9721.0742943035876125No Hit
ATTGCTGGGACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTC2360.2608368885253874No Hit
CGCAAGGGGAACTCAAAAAGAGGAAATTATGTTGAATATGGCTACAGTTAAT2310.255310683260019No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1820.2011538716594089No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1770.19562766639404056No Hit
CATTTTGGGTCTGCCTGCATATAAAGTAAAGGGAAAATAACTTGCAGAAGGA1560.17241760427949337No Hit
AGTTTCGGGATAAATTTTGTATTTTAAGGCAATTCTTCTAAATCTTAGTGGT1540.170207122173346No Hit
CGGTCCGGGTCCGATCTTGCTGAGGGTGGCCAGGCTGGTCTCAAACTCCTGA1500.1657861579610513No Hit
AGCCACGGGATATGGAAGTGGTAGAGATATAATTAAAGGTCATTTTGCTACT1490.16468091690797765No Hit
TGCATGCGGGAATTGATCAAATGATAGGTAAAGTATTTTAAAGTCTCTTTGT1410.15583898848338823No Hit
ATTGGAGGGACGGCATCGAATCCGGCAAAAACAGGAAGCTTTTTGGAGAAAA1400.15473374743031457No Hit
ATCATCGGGAAGATCAATTTAGCTAAGAGGTGAACCCAATAACCCCAAAGCC1370.15141802427109352No Hit
ACTCTGGGGAACGTTATGAAGACTGTCAACCTGTTTCCTCCTTACACTGTCA1360.15031278321801986No Hit
TTCAGTGGGGCAGTGTGTATTTGAAACATGTGCTCATTTTTATTCATTTTTA1300.1436813368995778No Hit
CCAGATGGGGAAAGAGAAATGAGTGGAGTTACAACAAGCATACTGACAATTT1260.13926037268728308No Hit
AACGGGGCAAGTAGAAGCCAAGCCCTCCGTCTATTTCATCAAGTTTGATGAT1260.13926037268728308No Hit
TTTTCTGGGCGATCTTGCTGAGGGATACCATGGAAATAGTAAGGGAGCAGGA1240.13704989058113576No Hit
GTAAAGGGTGTGGGGTTTTTGTGCATCTTATACTAATATGAAACAAACAAAA1200.13262892636884105No Hit
CGGCATGGGCTGAATTCTTTCTTTGTTTGGGTGCTTTCTATACTTAGAAATG1180.1304184442626937No Hit
AACACATTGGGTGCGCTTGTATATAGAAAAAATATATTAGTAATCTAAACAT1180.1304184442626937No Hit
GAAAAAGGGATGGGTGTCCTAGAAAAGCATACAGTCCAGAGCAGAAGGAGAC1180.1304184442626937No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1170.12931320320962003No Hit
CATACGGGGGTGCTGCTGGCCTGGGGTTGTGGTTGAGGCCGTGTCTCCGCTC1160.12820796215654634No Hit
TTTTAGCTGGGAAGGCACGTCGAGAAAGCAGGAAGTATACAAATCAACTTAA1140.125997480050399No Hit
TTGCTCGGGTGGTTATGAGCAGGTTGATGAGGGTCCTTCGGCCAGCACAACC1140.125997480050399No Hit
GTTTTTGGGAGCTTAGCTAGACCATGGTTGACTCTTCTTGGAGATTTTCACT1140.125997480050399No Hit
AGCCAGGGAGAGGGTGCATGCTGGAGCTCCATGGGCCTGAACTTGTCTTCCA1130.12489223899732531No Hit
GTTCGCGGGAATGACAAGAAATGAAAGGCAAGAAAAAATACATATGAAGAGA1100.12157651583810428No Hit
ATCTGGTTGGGAAAGTTACTTGTATCTGCTTATCATAAGACGAAACTCTGCA1090.12047127478503061No Hit
TGACTAGGGGTCAAAAGAGCTGGAAGGATTAATAAATACGAAAAGAAAGAGA1090.12047127478503061No Hit
TCTTCCGGGCATTTGGAAAATGAGATATTCTTCCTTCAGTTCATTGTGAGAG1080.11936603373195695No Hit
AAGCTAGGGACAACAGTGTTGAGCTGAGCCTTGATCTGATAAACACTTCCAG1080.11936603373195695No Hit
TGAACAGGGATTAGTAATAACGTTGAATGTAAATGGACTAAATCAAAAGACA1070.11826079267888326No Hit
TTAACAGGGATTGGGTATCTGTTGGGGTATAGGTCATTTTGAGTTGTTGTGG1070.11826079267888326No Hit
TAGATTGGGGCTAACGGAGGGATTGAGATACACATTTCTTAAAATATAAAGA1050.11605031057273592No Hit
TGTTAGGGGATCTCCAGGGACTCTCAGGCTAACCTTTGAGATCACTCAGCTC1050.11605031057273592No Hit
GACAGGTATTACTGGTTAGTTGTAAGAATATCAAAGTTAGTGAATAATTTGC1050.11605031057273592No Hit
TATTTAGGGAGTGCCAGGAAAACAGCTGAGAGGCATCTACCTTCATGGAAAC1050.11605031057273592No Hit
TACTGGGGGATTAGAAAAGCATCCACCTCCTCTTTTTAAAAAATATGTTGAT1040.11494506951966225No Hit
AAGCTAGGGAATGATTCTTTTAAAAAGTATTTTCAGTTATATACACATTTAC1030.11383982846658856No Hit
TATATCGGGATTTTTGAAAGCTCTACCTGAAGGGAATCTTAAAAGGAAAAAG1030.11383982846658856No Hit
TTTTTAGGGGATGTTCCTTACAGAGAAAATACTAAAGCCAGATCAGCCTTGT1020.11273458741351489No Hit
GTGGTTGGGGAATAAAACTGCTTTGATGCAGCTAGCAAAGTAAAATGAATAA1020.11273458741351489No Hit
TTACCAGGGGCATGAAACCAGGCTGCCTGGTTATGATCATCAGATCATAGTC1020.11273458741351489No Hit
ACTACTGGGTGGTAGAATGAGCAAATCAGGCTAATTAACATACCCGTCAATT1020.11273458741351489No Hit
TGCATTGGGAGGGAGAGAGGGATGGTTTCTGAGGGGTTCTGAAAGTATGATT1010.11162934636044122No Hit
CTCAGTGGGTGTATTTTTAATTAAAGTATAATTGCATGGAATTGGATTGAAT1000.11052410530736753No Hit
TGTTAAGGGATGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAA990.10941886425429387No Hit
AGTAGCGGGTCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCG990.10941886425429387No Hit
TATCTAGGGATGTCTTAAGGATGCCTTCATGACTTAAATCTGAAAAACTGTT990.10941886425429387No Hit
GCACAAGGGACCAGTCTCCTCCCCTACTCTGCCCCTAAGCTGGCTGTACCTG990.10941886425429387No Hit
GACACGGGGCACTTACAGTGATTGTACATATTGTACATCTTTGTACAGAGAC990.10941886425429387No Hit
GCTTTTGGGGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGGCTCAGGCA980.10831362320122019No Hit
ATCAAAGGGGACCATGGCCTCTCCCTGGGAACGGGCTGACCACAACACACCC970.1072083821481465No Hit
AAAATGGGGATATTAGGAGATGAGCAGAAAAATAACTTCCGACATAGTTTAC970.1072083821481465No Hit
CTTAGAGGGCTGCTGGCCAGCAGCACCCAGCGGAACGGGATCTGTGAGGTGC970.1072083821481465No Hit
GAGTGCGGGAGGACTCGCTAAGTGTTCGCTACGCGGGGCTACCGGATCGGTC970.1072083821481465No Hit
CATCCAGGGGAAACTTTTCAAGGCACATTGAAATACTTGAAAACTTCTCATT950.10499790004199916No Hit
TTGCTTGGGGATAATTTGATGTGCCTTGGGATGAAGGTATATCCCCCTGAGA950.10499790004199916No Hit
GAACCTGGGACTCTCTGAAATAAAAATAGAAACACACGTAGAAGTGAATATT950.10499790004199916No Hit
AGTGATGGGAAAGGAAAAGGTAAACCAATTCCTGTGGATTAGCAGTTCTGGT940.10389265898892548No Hit
ATACATGGGCAACAGCAACAACAACAGAACAACAACAAAGCATGCTCAGTAT940.10389265898892548No Hit
TTTAATGGGATTGTGCATCAAAGAACTCATACTGGAGAGAAACCCTTTACAT940.10389265898892548No Hit
GATAGGGGGGAAAAGAAGAAAAAAGAAACACGTATTCAAACTTACTGTTTAA940.10389265898892548No Hit
ATATCTGGGGTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGGG940.10389265898892548No Hit
ATGCCGGGTCACAACATAGCTAATCATGACAAAAGACTAGCCTAAGACAAAA940.10389265898892548No Hit
CTTCCTGGGAGTTGCGTTACACCCTTTCTTGACAAAACCTAACTTGCGCAGA930.1027874179358518No Hit
GCCATGGCACAGGAGGAAGAAACTGGGGCACAGATTGGGTCAATAACTTCAG930.1027874179358518No Hit
TGATACGGGACAAGAGAAAATCTTAAAAGCAGTACAGAAACATACACACACA930.1027874179358518No Hit
CAACTAGGGAGGCCCCAAACGGCCTCCGGTTGGTGGGCTCCTCTAGGCCCAG920.10168217688277814No Hit
GAAAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.10168217688277814No Hit
CATTTTGGGAAGGTAAATTGCACTTTTAAAAAAATAATTGGTTGACTTAATA920.10168217688277814No Hit
CTATAATAGGGAGGACTAGAGCTGCTAAGTTTACTGACTGTGGAATTACTAC920.10168217688277814No Hit
TTTCTTGGGAATGAAACTGAAGCATAGAGAAGAAAATGACTGAAAAAGTGAA920.10168217688277814No Hit
TACTATGGGAATTGACTTTTATAGATACTTTCTTACAGGAGATTCGAGGGGT910.10057693582970445No Hit
AGTCATGGGCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTG910.10057693582970445No Hit
AAGCTTGGGCAACATAGCAAGACTCCATTTCTACAAAGAACTTTTTAAAAAA910.10057693582970445No Hit
GTAAAGGGGTCAGTTTCCTGGGGTTCTTGCTGCTGTTGGTCACCTTCCCCAT910.10057693582970445No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)