FastQCFastQC Report
Sat 31 Dec 2022
SRR5848986.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848986.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90063
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC10251.1380922243318567No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT9431.0470448463853081No Hit
CGCAAGGGGAACTCAAAAAGAGGAAATTATGTTGAATATGGCTACAGTTAAT2420.26870079833005783No Hit
ATTGCTGGGACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTC2340.2598181273108824No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1960.21762543996979888No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1770.19652909629925716No Hit
AGTTTCGGGATAAATTTTGTATTTTAAGGCAATTCTTCTAAATCTTAGTGGT1590.1765430865061124No Hit
CATTTTGGGTCTGCCTGCATATAAAGTAAAGGGAAAATAACTTGCAGAAGGA1570.17432241875131851No Hit
CGGTCCGGGTCCGATCTTGCTGAGGGTGGCCAGGCTGGTCTCAAACTCCTGA1450.16099841222255534No Hit
AACGGGGCAAGTAGAAGCCAAGCCCTCCGTCTATTTCATCAAGTTTGATGAT1440.1598880783451584No Hit
CCAGATGGGGAAAGAGAAATGAGTGGAGTTACAACAAGCATACTGACAATTT1360.15100540732598292No Hit
TTTTCTGGGCGATCTTGCTGAGGGATACCATGGAAATAGTAAGGGAGCAGGA1330.14767440569379212No Hit
GAAAAAGGGATGGGTGTCCTAGAAAAGCATACAGTCCAGAGCAGAAGGAGAC1300.14434340406160134No Hit
TGCATGCGGGAATTGATCAAATGATAGGTAAAGTATTTTAAAGTCTCTTTGT1280.14212273630680744No Hit
ATTGGAGGGACGGCATCGAATCCGGCAAAAACAGGAAGCTTTTTGGAGAAAA1260.1399020685520136No Hit
CATACGGGGGTGCTGCTGGCCTGGGGTTGTGGTTGAGGCCGTGTCTCCGCTC1220.13546073304242587No Hit
AGCCACGGGATATGGAAGTGGTAGAGATATAATTAAAGGTCATTTTGCTACT1210.13435039916502892No Hit
TTTTAGCTGGGAAGGCACGTCGAGAAAGCAGGAAGTATACAAATCAACTTAA1200.133240065287632No Hit
AGTCATGGGCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTG1190.13212973141023507No Hit
GTGGTTGGGGAATAAAACTGCTTTGATGCAGCTAGCAAAGTAAAATGAATAA1170.1299090636554412No Hit
CTATAATAGGGAGGACTAGAGCTGCTAAGTTTACTGACTGTGGAATTACTAC1170.1299090636554412No Hit
AGCCAGGGAGAGGGTGCATGCTGGAGCTCCATGGGCCTGAACTTGTCTTCCA1160.12879872977804427No Hit
TGAACAGGGATTAGTAATAACGTTGAATGTAAATGGACTAAATCAAAAGACA1140.1265780620232504No Hit
TACTATGGGAATTGACTTTTATAGATACTTTCTTACAGGAGATTCGAGGGGT1140.1265780620232504No Hit
ATCATCGGGAAGATCAATTTAGCTAAGAGGTGAACCCAATAACCCCAAAGCC1140.1265780620232504No Hit
TGACTAGGGGTCAAAAGAGCTGGAAGGATTAATAAATACGAAAAGAAAGAGA1140.1265780620232504No Hit
TGTTAGGGGATCTCCAGGGACTCTCAGGCTAACCTTTGAGATCACTCAGCTC1130.12546772814585344No Hit
ATCAAAGGGGACCATGGCCTCTCCCTGGGAACGGGCTGACCACAACACACCC1130.12546772814585344No Hit
TACTGGGGGATTAGAAAAGCATCCACCTCCTCTTTTTAAAAAATATGTTGAT1110.12324706039105958No Hit
ATCTGGTTGGGAAAGTTACTTGTATCTGCTTATCATAAGACGAAACTCTGCA1100.12213672651366266No Hit
GATAGGGGGGAAAAGAAGAAAAAAGAAACACGTATTCAAACTTACTGTTTAA1100.12213672651366266No Hit
TGATACGGGACAAGAGAAAATCTTAAAAGCAGTACAGAAACATACACACACA1080.1199160587588688No Hit
CATTGTGGGGGGGTCTGGACGCAACGGCGGCGGGAGCATGAACGCCCCTCCA1070.11880572488147186No Hit
GTAAAGGGTGTGGGGTTTTTGTGCATCTTATACTAATATGAAACAAACAAAA1070.11880572488147186No Hit
GACAGGTATTACTGGTTAGTTGTAAGAATATCAAAGTTAGTGAATAATTTGC1070.11880572488147186No Hit
TGCATTGGGAGGGAGAGAGGGATGGTTTCTGAGGGGTTCTGAAAGTATGATT1070.11880572488147186No Hit
AGTTGCGGGACCCAGGTAGCCCGTGTGAAAAATATCATGTTGGTGATGTCCG1070.11880572488147186No Hit
TCTTCCGGGCATTTGGAAAATGAGATATTCTTCCTTCAGTTCATTGTGAGAG1060.11769539100407493No Hit
CATACAGGGAAGCACAGATTGAATTGTACAATTTGCAGATGCAGATGTAAAT1060.11769539100407493No Hit
CCACTTGGGAACCAAAGGAATCCTGAGCAAAAAGAACAAAGCTGAAGGCATC1050.116585057126678No Hit
TGACAAGGGCGATCTAAAAGCGGGTAATGTGGGAGTCAAAGTGAAGCTACAT1050.116585057126678No Hit
ACTCTGGGGAACGTTATGAAGACTGTCAACCTGTTTCCTCCTTACACTGTCA1050.116585057126678No Hit
CTAGATGGGTAGCAGAGAGAGAAAGAGATGAAATACACTTCGGTTTTCTCTT1040.11547472324928106No Hit
TTCAGTGGGGCAGTGTGTATTTGAAACATGTGCTCATTTTTATTCATTTTTA1040.11547472324928106No Hit
AAGCTAGGGACAACAGTGTTGAGCTGAGCCTTGATCTGATAAACACTTCCAG1040.11547472324928106No Hit
ATATCTGGGGTCCGGTCGCGGGACACCGGGTGTAGAGGGCGGTCGCGGCGGG1030.11436438937188413No Hit
CAACTTGGGACGACTTTTTTCTGGGACGAGAGCTCAACCCTCGTATCTGTTT1020.1132540554944872No Hit
GCTTTTGGGGTGGCTCATGCCTGTAATCCCAGCATTTTGGGAGGCTCAGGCA1010.11214372161709027No Hit
TTTAATGGGATTGTGCATCAAAGAACTCATACTGGAGAGAAACCCTTTACAT1000.11103338773969333No Hit
TAGAATGGGGCAGATGAGTTTGCCAAACTTCAGGATTATCGACAAGCAGAGG990.10992305386229641No Hit
ATTGCTGGGGGTTTTCACTGCATTTGGATCTCTTGAAGTTTTAAATCTCTTG980.10881271998489944No Hit
CTCAGTGGGTGTATTTTTAATTAAAGTATAATTGCATGGAATTGGATTGAAT980.10881271998489944No Hit
ACAACTGGGAGAAAACAAACTTGCTAATCACTTTACCAATGTTATCAAAATT980.10881271998489944No Hit
TTGCTCGGGTGGTTATGAGCAGGTTGATGAGGGTCCTTCGGCCAGCACAACC980.10881271998489944No Hit
TAGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA970.10770238610750252No Hit
AATTCAGGGATCTTGCATGATTCCCTCCTAAATATATTCCTTGATTAAGTAA970.10770238610750252No Hit
TATTTAGGGAGTGCCAGGAAAACAGCTGAGAGGCATCTACCTTCATGGAAAC970.10770238610750252No Hit
TTGAACCGGGGAAGACATTTTATTTTAAATTTTTAATTTGAGGGGAAAAGAA970.10770238610750252No Hit
TACATAGCGGATGGCTTCAGGTCTGACCCAGTGCATTCCTAATGGTGGTGGC960.10659205223010558No Hit
TATCTAGGGATGTCTTAAGGATGCCTTCATGACTTAAATCTGAAAAACTGTT960.10659205223010558No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA960.10659205223010558No Hit
TTGCGCGGGTAACCAAAACAGCATGGCACTGGCATAAAAATAGACACATAGA960.10659205223010558No Hit
TGTGCGGGAGTGCAGTGACATTACCACAGGGATTGTTGCAAGTTAGTCTAGA950.10548171835270866No Hit
TCTATTGGGATCTTAAGTATGACCTCATGCCTTTTTGAGATTATCTTTTGTA950.10548171835270866No Hit
GGTGATGGGACTAAAAGATGCAATTCAAAGATAGGTCCCAGTTTAACACTGA950.10548171835270866No Hit
GTTTTTGGGAGCTTAGCTAGACCATGGTTGACTCTTCTTGGAGATTTTCACT950.10548171835270866No Hit
TAGCAAGGGGAGGCTTTAGGTCACTGTTTCACTTCAAAGTACTGGATCCAAA940.10437138447531172No Hit
TGCGCGGGGCAGCAACTTAGACTCTCCACGTGAATTGGGGAAAACAACATGT940.10437138447531172No Hit
CGAGCTGGGAAAGAGAGGGAGTTGACTGGCTTTTAAAAAGTATTTAAATACC940.10437138447531172No Hit
GCAGCAGGGCAGTTGTTAAGAGGTTTTGGTTCCAAACTCAACCAGCAATGTT940.10437138447531172No Hit
TTTCTTGGGAATGAAACTGAAGCATAGAGAAGAAAATGACTGAAAAAGTGAA940.10437138447531172No Hit
CGGCATGGGCTGAATTCTTTCTTTGTTTGGGTGCTTTCTATACTTAGAAATG930.1032610505979148No Hit
TATGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.1032610505979148No Hit
GACACGGGGCACTTACAGTGATTGTACATATTGTACATCTTTGTACAGAGAC930.1032610505979148No Hit
GTTCGCGGGAATGACAAGAAATGAAAGGCAAGAAAAAATACATATGAAGAGA920.10215071672051786No Hit
CATAGAGGGATCTACTTAAAAATGGGTGGTAATCTTTTAAAATGTAGCTTAG920.10215071672051786No Hit
AGTTACGGGATTGCAACATTGTTCATAAAAGCCAAGATTTGGAAGCAACCTA920.10215071672051786No Hit
TTTTTAGGGGATGTTCCTTACAGAGAAAATACTAAAGCCAGATCAGCCTTGT910.10104038284312093No Hit
AAAATGGGGATATTAGGAGATGAGCAGAAAAATAACTTCCGACATAGTTTAC910.10104038284312093No Hit
AACACATTGGGTGCGCTTGTATATAGAAAAAATATATTAGTAATCTAAACAT910.10104038284312093No Hit
TCACTTGGGAGATACATTTTAGTGATTGTTTTATTAACTCTATAATACCTCT910.10104038284312093No Hit
TTTCCAGGGGGGTTCAAGCTTTCCTATTAGCCATGGAAATGCAAAGTTTAGC910.10104038284312093No Hit
TATGGTGGGCAGAGGTATTTTCATTTTTACTTTTTGATTAATTGTGTTTTAT910.10104038284312093No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)