FastQCFastQC Report
Sat 31 Dec 2022
SRR5848985.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848985.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91141
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGC10801.1849771233583128No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCGTCT9221.0116193590151523No Hit
CGCAAGGGGAACTCAAAAAGAGGAAATTATGTTGAATATGGCTACAGTTAAT2390.2622310485950341No Hit
ATTGCTGGGACAGTGCCAGTGGTTGATGTACGCATCCCTCAACTAGCACCTC2290.2512590381935682No Hit
CATTTTGGGTCTGCCTGCATATAAAGTAAAGGGAAAATAACTTGCAGAAGGA2020.22163461010961039No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATATATCTCGTATGCCTGTC1910.20956539866799792No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATAT1820.19969058930667866No Hit
TGCATGCGGGAATTGATCAAATGATAGGTAAAGTATTTTAAAGTCTCTTTGT1460.16019135186140157No Hit
AACGGGGCAAGTAGAAGCCAAGCCCTCCGTCTATTTCATCAAGTTTGATGAT1390.15251094458037545No Hit
ATTGGAGGGACGGCATCGAATCCGGCAAAAACAGGAAGCTTTTTGGAGAAAA1340.14702493937964253No Hit
AGTTTCGGGATAAATTTTGTATTTTAAGGCAATTCTTCTAAATCTTAGTGGT1310.14373333625920276No Hit
ATCATCGGGAAGATCAATTTAGCTAAGAGGTGAACCCAATAACCCCAAAGCC1310.14373333625920276No Hit
GAAAAAGGGATGGGTGTCCTAGAAAAGCATACAGTCCAGAGCAGAAGGAGAC1310.14373333625920276No Hit
AGCCACGGGATATGGAAGTGGTAGAGATATAATTAAAGGTCATTTTGCTACT1270.13934453209861644No Hit
AGCCAGGGAGAGGGTGCATGCTGGAGCTCCATGGGCCTGAACTTGTCTTCCA1250.13715013001832327No Hit
GTAAAGGGTGTGGGGTTTTTGTGCATCTTATACTAATATGAAACAAACAAAA1190.13056692377744375No Hit
TTTTAGCTGGGAAGGCACGTCGAGAAAGCAGGAAGTATACAAATCAACTTAA1180.12946972273729715No Hit
CCAGATGGGGAAAGAGAAATGAGTGGAGTTACAACAAGCATACTGACAATTT1180.12946972273729715No Hit
TGTTAGGGGATCTCCAGGGACTCTCAGGCTAACCTTTGAGATCACTCAGCTC1130.12398371753656423No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGGGAATATA1130.12398371753656423No Hit
CTATAATAGGGAGGACTAGAGCTGCTAAGTTTACTGACTGTGGAATTACTAC1130.12398371753656423No Hit
CGGTCCGGGTCCGATCTTGCTGAGGGTGGCCAGGCTGGTCTCAAACTCCTGA1110.12178931545627106No Hit
GATAGGGGGGAAAAGAAGAAAAAAGAAACACGTATTCAAACTTACTGTTTAA1110.12178931545627106No Hit
TTCAGTGGGGCAGTGTGTATTTGAAACATGTGCTCATTTTTATTCATTTTTA1100.12069211441612447No Hit
GCCATGGCACAGGAGGAAGAAACTGGGGCACAGATTGGGTCAATAACTTCAG1090.11959491337597788No Hit
TCAATAGGGATACTAATGCTGCAACACTTAAAGAATGCCTCTAATGTCAGCT1080.1184977123358313No Hit
ACAGTAGGGCGATCTAGAGGAGGGTAAGAAAAAGCGAAGGAATTTCGCAGAG1080.1184977123358313No Hit
TTTTCTGGGCGATCTTGCTGAGGGATACCATGGAAATAGTAAGGGAGCAGGA1080.1184977123358313No Hit
TCACTTGGGAGATACATTTTAGTGATTGTTTTATTAACTCTATAATACCTCT1070.11740051129568471No Hit
CTAGATGGGTAGCAGAGAGAGAAAGAGATGAAATACACTTCGGTTTTCTCTT1060.11630331025553813No Hit
AAGCTAGGGAATGATTCTTTTAAAAAGTATTTTCAGTTATATACACATTTAC1060.11630331025553813No Hit
CCTGATGGGATTGCCTTAAATAAAATCACATTTCATTTCTTGTTTTATACAT1060.11630331025553813No Hit
TAGCAAGGGGAGGCTTTAGGTCACTGTTTCACTTCAAAGTACTGGATCCAAA1050.11520610921539154No Hit
AGTCATGGGCCGACTTTCCTTGGCTACTCCATGCAGGGACTCTGCAAGTTTG1050.11520610921539154No Hit
CTTGCGGGGGTCCTCGGCCCCAGGAGTCTCCTCAACTGACACTGAAGTGGTG1050.11520610921539154No Hit
TGCGAATGGGTAGCAATATTAATAGTACCAATTCAGGAATGAGTAATATTTT1050.11520610921539154No Hit
GTACAGGGGTACTGTCGTTTTATTTCTAAATTTCCTTTCAAACTCATCAAGC1030.11301170713509837No Hit
CTTAGAGGGCTGCTGGCCAGCAGCACCCAGCGGAACGGGATCTGTGAGGTGC1030.11301170713509837No Hit
AACACATTGGGTGCGCTTGTATATAGAAAAAATATATTAGTAATCTAAACAT1030.11301170713509837No Hit
ACTCTGGGGAACGTTATGAAGACTGTCAACCTGTTTCCTCCTTACACTGTCA1030.11301170713509837No Hit
TATATCGGGATTTTTGAAAGCTCTACCTGAAGGGAATCTTAAAAGGAAAAAG1030.11301170713509837No Hit
TGAACAGGGATTAGTAATAACGTTGAATGTAAATGGACTAAATCAAAAGACA1020.11191450609495178No Hit
ATACATGGGCAACAGCAACAACAACAGAACAACAACAAAGCATGCTCAGTAT1020.11191450609495178No Hit
CTCAGTGGGTGTATTTTTAATTAAAGTATAATTGCATGGAATTGGATTGAAT1020.11191450609495178No Hit
TCTTCCGGGCATTTGGAAAATGAGATATTCTTCCTTCAGTTCATTGTGAGAG1020.11191450609495178No Hit
TGACTAGGGGTCAAAAGAGCTGGAAGGATTAATAAATACGAAAAGAAAGAGA1020.11191450609495178No Hit
AGTTGCGGGACCCAGGTAGCCCGTGTGAAAAATATCATGTTGGTGATGTCCG1020.11191450609495178No Hit
TACTATGGGAATTGACTTTTATAGATACTTTCTTACAGGAGATTCGAGGGGT1010.11081730505480519No Hit
TGCATGGGGGCATGTTTTAAATTGTAAATGGAGCTAAGTCCAAGAAAGTACT1010.11081730505480519No Hit
GACAGGTATTACTGGTTAGTTGTAAGAATATCAAAGTTAGTGAATAATTTGC1010.11081730505480519No Hit
CATACAGGGAAGCACAGATTGAATTGTACAATTTGCAGATGCAGATGTAAAT1010.11081730505480519No Hit
AAATGCGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1000.1097201040146586No Hit
AGTAGCGGGTCAGGCGGCGGCGCAGGCGGCGGCGGCGGCATAGCGCACAGCG1000.1097201040146586No Hit
TGGTATTGGGATGCTGCTAAATGAAAGAAAGAAAAAAGCAAATTATTTTTTT990.10862290297451202No Hit
TTGCTCGGGTGGTTATGAGCAGGTTGATGAGGGTCCTTCGGCCAGCACAACC990.10862290297451202No Hit
AAGCTAGGGACAACAGTGTTGAGCTGAGCCTTGATCTGATAAACACTTCCAG990.10862290297451202No Hit
CATCCAGGGGAAACTTTTCAAGGCACATTGAAATACTTGAAAACTTCTCATT980.10752570193436543No Hit
CGTTCAGGGAATGAAGGCTGTATATGAAAAGGGCACAGCTGACATCACACTC980.10752570193436543No Hit
TGTGCGGGAGTGCAGTGACATTACCACAGGGATTGTTGCAAGTTAGTCTAGA970.10642850089421885No Hit
CTATCCGGGATCAGAATACTCTAGATAGTTCACTTCTACATTTAATAAGTAT970.10642850089421885No Hit
GTTTTTGGGAGCTTAGCTAGACCATGGTTGACTCTTCTTGGAGATTTTCACT960.10533129985407226No Hit
TCAGTGGGGGAATTGATGACTTGCGATACCAGATAGATGATAAACCAAACAA950.10423409881392567No Hit
ACTCATGGGAGATTTCAAATATATAATAGAATGTCGTTTACTAAAGTCACAT950.10423409881392567No Hit
AAGCTTGGGCAACATAGCAAGACTCCATTTCTACAAAGAACTTTTTAAAAAA950.10423409881392567No Hit
TATCTAGGGATGTCTTAAGGATGCCTTCATGACTTAAATCTGAAAAACTGTT940.10313689777377909No Hit
GTGGTTGGGGAATAAAACTGCTTTGATGCAGCTAGCAAAGTAAAATGAATAA930.1020396967336325No Hit
ACTCATGGGATACTTCTTTGCTATTTCCACCTATGTGCAGAGGATTATCATT930.1020396967336325No Hit
ATTGCTGGGAAGAGGGCTGGTCTCATAACTAAAAGGTCCAGTCCCTGAGATC930.1020396967336325No Hit
TGTTAAGGGATGATATACGGTATCAGAGACTGGTGGTGGAGCTGCTTATCAA920.10094249569348591No Hit
ATCTTAGGGGCCACAAACATGTATGTGTTTCTTTTCTTTATTTTTTCTTTAT920.10094249569348591No Hit
TACTGGGGGATTAGAAAAGCATCCACCTCCTCTTTTTAAAAAATATGTTGAT920.10094249569348591No Hit
GACGGCTTTGGTTTAAGTCTTAACTCTGGCTTCCTAGGAGCTGTGTGACACT920.10094249569348591No Hit
TTTCTTGGGAATGAAACTGAAGCATAGAGAAGAAAATGACTGAAAAAGTGAA920.10094249569348591No Hit
GGCATGGGGAACCTCTAAATTTCAAAATTATGAAGAATTACAGAATTACTCA920.10094249569348591No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)