FastQCFastQC Report
Sat 31 Dec 2022
SRR5848981.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848981.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165170
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT8370.5067506205727432No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC7810.4728461585033602No Hit
GTTGCGGGATACTGTTGGAGTCTGAGAGTTTCGATTAATAGAAATTTATGGT2380.144093963794878No Hit
CGCTTTGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG2120.1283526064055216No Hit
GCTAGTGGGATATCTTCTGTAAACATAGTGTAGTTATTTAAAACAGGAAATC2090.12653629593751892No Hit
TGAAATGGGTCAACAAGAAGTTATGCCACTTCTTATAAGCTCAATTAATAAG2050.12411454864684869No Hit
CTTATGTCGGGATTGACCACATACTTGGAAGTAAAACACTCCTCAGCAAATG2010.12169280135617849No Hit
TCACGGAGGGATCCAATGAGCTTATCAAAATATTCATTCAAATTACTTGGAG1990.12048192771084339No Hit
TATGTAGGGTTATTTTTTTAGTTTTATTTTGCACGTGTAGAGTTTTTGTCAT1980.11987649088817583No Hit
GGGAATGGGAGAAGAAAAGATCCTGTTTCCATTTGAAAGGAACTGTAAGCTT1880.11382212266150028No Hit
ATTTCGGGGGCTGGAAAACTCTGGGAACTGCAGCACGAGATCGAGGTGTACC1880.11382212266150028No Hit
TATGACGGGAATGTCTGACTGTTAATTCTTAAGCAAGAAACTTTTTTTGATG1870.11321668583883272No Hit
TGCAGCGGGGAAAGTGAGAAGTAACGGTTTTTGATCTGGTTCACTTGTGTAT1870.11321668583883272No Hit
GGTGATGGGTCTTTCAGCTCACACATGACAGAGTTTCCACGAAAACGCAAAG1870.11321668583883272No Hit
CTTGTTGGGTCTCCGGGGGAGGCTGTGATAAACATTTCCGGAGCACTTGCAG1860.11261124901616516No Hit
GGATGCGGGGAAAAAATGCAATTTGAGACATTGCGCCAGGTCTGCAATTCTG1850.11200581219349762No Hit
GCTCAAGGGTGATTCTGCTGGTAACTGACAACTGACCGAGTCTCCATGGTTC1840.11140037537083006No Hit
TGACATGGGTATACCTGACACTTAATATGGAGACTAATGCATATGAGGTGCT1840.11140037537083006No Hit
ACAAGACTTTTTATGTGATCTTGACCAAACCACTGCACTTGATTGCTCCTTT1840.11140037537083006No Hit
CAGAAAGGGAACAGTGATTGGCCTAGAAAGGCACTTTCAAATACATGTCAAT1820.11018950172549495No Hit
CTTGCCGGGGTGATACCTATTTGGGTAAGGAATATACATACAGAGACAAGTA1820.11018950172549495No Hit
CCCTTGGGAACTTTGTAGAACCATCCTTTCTAGATGGGTCCACCACAGTGAA1820.11018950172549495No Hit
TACGCCAGGGAGCGCAGGTATTGTATTTGAATCCATCTTATGAAAGGTGGTC1810.10958406490282739No Hit
GCTTGAGGGCATACCTACTTTGATGGCTTGTGCGTGAGCCTTATTCTCATCT1800.10897862808015983No Hit
ATATGTGGGTCCGATCTGCGTGTGGGGGACGGCCCCCGAGGCAGCCCTGTAC1800.10897862808015983No Hit
TGTCTTGGGCTGAAGCAATGAACACCATGCCTGTGCAAATGCCCAGAAAGAT1800.10897862808015983No Hit
GTCAACGGGGAGGGATTCAGTTTAATGGAATAAAAGCCAAGTTTGGAAAAGC1770.10716231761215718No Hit
AACGATGGAATATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGC1770.10716231761215718No Hit
TAAGCTCGGGGAGCTTAGAGATGTGAAGGGTTGATTGTATGTATCTGTTTCC1760.10655688078948962No Hit
ATACAAAGGGGTTTCCCATGATCTTGTTTGATGTTAGAGCAACTGATTCAGT1740.10534600714415451No Hit
GAGATTGGGGTTCCTCCACATTAGGAAGCAAATACTTGATTGATTTGATATT1730.10474057032148695No Hit
GACTAGGGGTCTTCCAAGGGGTATTGTAATAAAGCTTTTACTACTATGAAGG1720.1041351334988194No Hit
CTTACAGGGTAAGACAAACGAACACATTAACCCAGGCCTACACAGCACCAGG1710.10352969667615185No Hit
AAACAGAGGGAAATGTCGGCTTTTAGATTACCTTGAAAACATAGCAGCATAA1700.10292425985348429No Hit
CGTTTAGGGAACCAATGACTTAATTTAACTTAGTGGGTATACTGTTCCCAGT1690.10231882303081673No Hit
TTATTTGGGAGGATTGTGGGACATGGGGAGCTTTTTCCTCCCTAAAAGGGGA1690.10231882303081673No Hit
GTTCTAGGGAGACAATGGTTAGAGAAGAATTTTCCAAATGAATTTGCAAAAC1670.10110794938548162No Hit
GAGTTCGGGGGGCTCTGCACAAACACATTTTGGTGGGAGGCTGGGGTCTATC1670.10110794938548162No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)