FastQCFastQC Report
Sat 31 Dec 2022
SRR5848980.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848980.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164550
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC8020.48738985110908534No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT7550.45882710422364026No Hit
GGATGCGGGGAAAAAATGCAATTTGAGACATTGCGCCAGGTCTGCAATTCTG2100.12762078395624432No Hit
TGCAATGGGATCCCAGTGATTTACGAAACTGTGTCAGTTCCCTGAAACATAA2020.12275903980553023No Hit
AAACAGAGGGAAATGTCGGCTTTTAGATTACCTTGAAAACATAGCAGCATAA2000.12154360376785171No Hit
GTTGCGGGATACTGTTGGAGTCTGAGAGTTTCGATTAATAGAAATTTATGGT1980.12032816773017319No Hit
TAAGCTCGGGGAGCTTAGAGATGTGAAGGGTTGATTGTATGTATCTGTTTCC1980.12032816773017319No Hit
CTTATGTCGGGATTGACCACATACTTGGAAGTAAAACACTCCTCAGCAAATG1980.12032816773017319No Hit
CGCTTTGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG1980.12032816773017319No Hit
GCTAGTGGGATATCTTCTGTAAACATAGTGTAGTTATTTAAAACAGGAAATC1960.11911273169249467No Hit
CTTGTTGGGTCTCCGGGGGAGGCTGTGATAAACATTTCCGGAGCACTTGCAG1930.1172895776359769No Hit
TGAAATGGGTCAACAAGAAGTTATGCCACTTCTTATAAGCTCAATTAATAAG1920.11668185961713765No Hit
AGATAGGGGGGTAGAGGAAGAGGCTGAGGAACCCTACGAAGAAGCCACAGAG1880.1142509875417806No Hit
GGGAATGGGAGAAGAAAAGATCCTGTTTCCATTTGAAAGGAACTGTAAGCTT1860.11303555150410209No Hit
TTATTTGGGAGGATTGTGGGACATGGGGAGCTTTTTCCTCCCTAAAAGGGGA1860.11303555150410209No Hit
GTTCTGGGACTCTTGGAACAGCAGAGAAGTCCAGAATAGCTTCAGACATGTT1840.11182011546642358No Hit
TGTCTTGGGCTGAAGCAATGAACACCATGCCTGTGCAAATGCCCAGAAAGAT1840.11182011546642358No Hit
AAGCGGGTGTGCCTCAGAGTTTCCATCTTTAAAGTAAGGAATTGTAGTAGGA1830.11121239744758431No Hit
GCTCCGGGGCTCGTGTCTGTGGTCTGTTTTAAGTTTGGGTGTGCAAGGCCAA1830.11121239744758431No Hit
TGTCAAGGGCTAGAAGGCATCGCAGTGCTAAACATCCCTAGCATGCATGGTG1820.11060467942874505No Hit
GAGATTGGGGTTCCTCCACATTAGGAAGCAAATACTTGATTGATTTGATATT1820.11060467942874505No Hit
CTTTACGGGAAGAGTACAACTGAATAATGATGTTCTTTTTCATCAAGTTCCT1820.11060467942874505No Hit
TATGACGGGAATGTCTGACTGTTAATTCTTAAGCAAGAAACTTTTTTTGATG1810.1099969614099058No Hit
CAATCCGGGCGTGGAGTGGATCCCCAACAACGTGAAGACGGCCGTGTGCGAC1810.1099969614099058No Hit
TGCAGCGGGGAAAGTGAGAAGTAACGGTTTTTGATCTGGTTCACTTGTGTAT1790.10878152537222728No Hit
TCACGGAGGGATCCAATGAGCTTATCAAAATATTCATTCAAATTACTTGGAG1790.10878152537222728No Hit
CATGACGGGGTGGGTTTGGCATTGAGCCTCAAGACACTCACTACATTCTTTC1780.10817380735338802No Hit
CAGAAAGGGAACAGTGATTGGCCTAGAAAGGCACTTTCAAATACATGTCAAT1760.10695837131570951No Hit
GCTTGAGGGCATACCTACTTTGATGGCTTGTGCGTGAGCCTTATTCTCATCT1750.10635065329687024No Hit
AACCGAGGGATCCAGAGAGGCTCTCTCAAAGATCAGGGAGATGTATTCCCAT1750.10635065329687024No Hit
AACGATGGAATATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGC1750.10635065329687024No Hit
GTTAGTGGGTCACCAGTAGTAGATTTAGTTTAGAACACCACTTTCTTTGCTC1740.10574293527803098No Hit
AACTCCGGGGATTAAAGGCCTCATTACCTCTCCTAGAGGAATGATGAGCTGC1740.10574293527803098No Hit
ATATGTGGGTCCGATCTGCGTGTGGGGGACGGCCCCCGAGGCAGCCCTGTAC1740.10574293527803098No Hit
TAATTGGGGAGATGAATGAATTTGAAACCCTGACTGCAAAGTTCCATTTTGT1730.10513521725919173No Hit
GCGCAGGGGCAATGGGATATCCAACAGGCTTTGATGCAGATATTGAATGTAT1730.10513521725919173No Hit
CTCAAAGGGCTGCTTATTAATTTAGCCATAACTTTGAAAGTTTACGTTAAGA1720.10452749924035247No Hit
CTAACGCGGGGGGCTCTGACTAAAAAGGAACCTGCCTAAAGTTTGGTCAAGG1710.10391978122151321No Hit
TGTAGCGGGGTGTTTTTCTCTGAACACTGCTTAAGATTCTATCTGTACCTCT1700.10331206320267396No Hit
ATACAAAGGGGTTTCCCATGATCTTGTTTGATGTTAGAGCAACTGATTCAGT1690.1027043451838347No Hit
TTGCTGGGGAATCACTGCTGAAGATGCCCCTGCTCCCTCTGGGTTCCAGCAG1680.10209662716499544No Hit
CGTATAGGGGCCTAGGGGAAGTATTCTGGTTTCTTAGGTGATGGGTGGGGCT1680.10209662716499544No Hit
AACTCCGGGATGGAATCAATCTTTAACAACCTCTGAGCCTTGGTGTACAGAC1680.10209662716499544No Hit
ATTTCGGGGGCTGGAAAACTCTGGGAACTGCAGCACGAGATCGAGGTGTACC1680.10209662716499544No Hit
GGTGATGGGTCTTTCAGCTCACACATGACAGAGTTTCCACGAAAACGCAAAG1670.10148890914615617No Hit
TACGCCAGGGAGCGCAGGTATTGTATTTGAATCCATCTTATGAAAGGTGGTC1660.10088119112731692No Hit
AAATGAGGGGAATCTGTGCACTTGGCGGGACCCAGAACACACAGTGGCAGGG1660.10088119112731692No Hit
CACGGGGGGTTTTTGAACTAATTGAAATTATGTGTAAAGTGTTCCACATAAA1650.10027347310847766No Hit
GTCAACGGGGAGGGATTCAGTTTAATGGAATAAAAGCCAAGTTTGGAAAAGC1650.10027347310847766No Hit
GACTAGGGGTCTTCCAAGGGGTATTGTAATAAAGCTTTTACTACTATGAAGG1650.10027347310847766No Hit
GGTACAGGGAGAGCAGTGAATCATTTTATGAATAATAATGCTGGATTTTATT1650.10027347310847766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)