FastQCFastQC Report
Sat 31 Dec 2022
SRR5848979.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848979.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162996
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC7930.4865150065032271No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT7750.47547179071879064No Hit
GTTGCGGGATACTGTTGGAGTCTGAGAGTTTCGATTAATAGAAATTTATGGT2280.13988073326952807No Hit
CTTATGTCGGGATTGACCACATACTTGGAAGTAAAACACTCCTCAGCAAATG2220.13619966134138262No Hit
CTTGTTGGGTCTCCGGGGGAGGCTGTGATAAACATTTCCGGAGCACTTGCAG2090.12822400549706742No Hit
TGAAATGGGTCAACAAGAAGTTATGCCACTTCTTATAAGCTCAATTAATAAG2050.12576995754497042No Hit
GGATGCGGGGAAAAAATGCAATTTGAGACATTGCGCCAGGTCTGCAATTCTG1960.1202483496527522No Hit
GCTCAAGGGTGATTCTGCTGGTAACTGACAACTGACCGAGTCTCCATGGTTC1820.11165918182041276No Hit
GTCAACGGGGAGGGATTCAGTTTAATGGAATAAAAGCCAAGTTTGGAAAAGC1810.11104566983238852No Hit
GGGAATGGGAGAAGAAAAGATCCTGTTTCCATTTGAAAGGAACTGTAAGCTT1810.11104566983238852No Hit
CAATCCGGGCGTGGAGTGGATCCCCAACAACGTGAAGACGGCCGTGTGCGAC1810.11104566983238852No Hit
ATATGTGGGTCCGATCTGCGTGTGGGGGACGGCCCCCGAGGCAGCCCTGTAC1800.11043215784436428No Hit
CAGAAAGGGAACAGTGATTGGCCTAGAAAGGCACTTTCAAATACATGTCAAT1790.10981864585634003No Hit
GTCTGTGGGGAGCATACCCAAGATGACTGACTCATTACATTGACAGAAAAAC1790.10981864585634003No Hit
TTATTTGGGAGGATTGTGGGACATGGGGAGCTTTTTCCTCCCTAAAAGGGGA1780.10920513386831578No Hit
AACGATGGAATATCTAGTTCTCAGAGCATTTGGAAAGGTTATCTTAAATGGC1770.10859162188029153No Hit
TGTCAAGGGCTAGAAGGCATCGCAGTGCTAAACATCCCTAGCATGCATGGTG1760.10797810989226729No Hit
CTTTACGGGAAGAGTACAACTGAATAATGATGTTCTTTTTCATCAAGTTCCT1750.10736459790424305No Hit
TATGACGGGAATGTCTGACTGTTAATTCTTAAGCAAGAAACTTTTTTTGATG1740.1067510859162188No Hit
AAGCATGGACTTCAACATAATGAAGGCTGTATATGAAAAGGGCACAGCTGAC1740.1067510859162188No Hit
AAGCAGGGGCGTGTATGACTCAGTTGCTTTCCCTGAGCAGGACGGGAGCCCC1720.10552406194017032No Hit
CGCTGTGGGGAGAGGGGCATGACATATTTACGGTGCTGAAAGAAAAAAACTT1710.10491054995214608No Hit
TATGTAGGGTTATTTTTTTAGTTTTATTTTGCACGTGTAGAGTTTTTGTCAT1710.10491054995214608No Hit
TCACGGAGGGATCCAATGAGCTTATCAAAATATTCATTCAAATTACTTGGAG1710.10491054995214608No Hit
ACTATAGGGGCTCTGAAGTGATCTATTTATTTTTTCAGGAAATTCATCTCCA1690.10368352597609758No Hit
TGCAGCGGGGAAAGTGAGAAGTAACGGTTTTTGATCTGGTTCACTTGTGTAT1690.10368352597609758No Hit
AAACAGAGGGAAATGTCGGCTTTTAGATTACCTTGAAAACATAGCAGCATAA1680.10307001398807333No Hit
GCTCCGGGGCTCGTGTCTGTGGTCTGTTTTAAGTTTGGGTGTGCAAGGCCAA1680.10307001398807333No Hit
AGAATAGGGGATAACATTCCGGCTAAGAAACTGGAGCTTTTGACAAGGAGCC1670.10245650200004909No Hit
TGACATGGGTATACCTGACACTTAATATGGAGACTAATGCATATGAGGTGCT1670.10245650200004909No Hit
ATCGTCGGGATTTGACTACTCCAAAAGAAGTTAAGAAAAGGCAGAAAGGGAC1670.10245650200004909No Hit
GAATATGGGAGAAGGGGTCCACGTTGAATTCTGAATCATCTTGAAAATAAGA1660.10184299001202482No Hit
ATTTCGGGGGCTGGAAAACTCTGGGAACTGCAGCACGAGATCGAGGTGTACC1660.10184299001202482No Hit
TAATTGGGGAGATGAATGAATTTGAAACCCTGACTGCAAAGTTCCATTTTGT1660.10184299001202482No Hit
TGCAATGGGATCCCAGTGATTTACGAAACTGTGTCAGTTCCCTGAAACATAA1650.10122947802400058No Hit
GCTTGAGGGCATACCTACTTTGATGGCTTGTGCGTGAGCCTTATTCTCATCT1650.10122947802400058No Hit
CGCTTTGGGAGACGCGCCGGAACCGGGACGCGATAAATATGCAGAGCGGAGG1650.10122947802400058No Hit
ACAATTGGGGCAGGGATGAGGCAGGGAATGTTAATGAGTAAAATAGGTCAGG1630.10000245404795209No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)