FastQCFastQC Report
Sat 31 Dec 2022
SRR5848969.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848969.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences167206
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6960.41625300527493037No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5850.34986782770953195No Hit
GCAAATGGGACCCCTCCTCCAGTGCAATGTCATCATAGACTGTCCATGACAG2020.12080906187577001No Hit
GCTCGCGGGGGGACCATGGCGCAGGGGCAGCGTAAGTTTCAGGCGCACAAAC1920.11482841524825664No Hit
TTGCACTGGGATGGTAAATTTCTCTTACATGCTCAACTTCCTGCATGTAAAA1920.11482841524825664No Hit
ATGGCTGGCTCCAAAGTAAATGAAATACTTAGGTATAAACCTAACAAAAAAA1910.1142303505855053No Hit
GATTTAGGGAGCTTTATGGATTCTATTATTTAAGATTTGCAACTGATTTCAG1860.11124002727174861No Hit
ATGCATGGGGAGACTCGAGCAGTCTCTGGAACACGCTGCGGGGCTCCCGGGC1850.11064196260899728No Hit
ATGTACGGGATAAAATAATTTGCTATTTATTAAATAAAAATTCTAAATGTTT1830.10944583328349462No Hit
TCGCTGGGACTTTAATGTTGATCTTGAAGAAATCAGAGAAGTCGTATCAAAG1820.10884776862074327No Hit
ACTACGGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCG1820.10884776862074327No Hit
GTACTGGGACTACATGGGAAGAAAAGATAGAGCCTGGTTGGTAACGATTATA1820.10884776862074327No Hit
GGCTTTGGGGCTTCCAGGTTACAATTGCTTGCTTTCTCATTGTCAGTAAGCA1790.10705357463248925No Hit
GCCAGTGGGTAGAGAAAAAGTATAAATGATTGACCTGACCTGACAGTATTCA1780.10645550996973793No Hit
CTTTTGCGGGGATTCCATATTTGCCTAACGGCAATGTGGTGAAAGGTACTCA1770.1058574453069866No Hit
AGTGCTGGGAATTTGTCCTCTAGAAAATATCCAAAACAGGGTCACAAAATGA1770.1058574453069866No Hit
AGCTTTGGGCACTTATTGGAATGTATCTAATGTTTTTCTCAAGATAAGACTG1770.1058574453069866No Hit
AACGCAGGGATTACCAGTAATGATGATAAGCACAGCTACATTTAGTTGAACC1740.10406325131873259No Hit
GGCTGTGGGGCCCAGTCTACATGTACGATTTTTAACAGGCGATGTTGTTGAA1720.1028671219932299No Hit
ACACACGGGATCTGAAAGAACCAGGGGGCTGAGGCACACAGGAAACTGGAGA1720.1028671219932299No Hit
TGTGATGGGGATAAGTAGTCTGTGGGGAGTCCCATATTTTTCTTACCTTAGA1690.10107292800497589No Hit
TCGACTGGGTAGATACTGATTTATATTTCTTAAATTACATTTGCAAATGAAG1680.10047486334222457No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)