FastQCFastQC Report
Sat 31 Dec 2022
SRR5848968.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848968.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166698
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6980.4187212803992849No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5170.31014169336164804No Hit
ATGCATGGGGAGACTCGAGCAGTCTCTGGAACACGCTGCGGGGCTCCCGGGC2210.13257507588573347No Hit
AGCTTTGGGCACTTATTGGAATGTATCTAATGTTTTTCTCAAGATAAGACTG2140.12837586533731657No Hit
TTGCACTGGGATGGTAAATTTCTCTTACATGCTCAACTTCCTGCATGTAAAA1950.11697800813447072No Hit
ACTACGGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCG1900.11397857202845865No Hit
GATTCAGCCTCCAGACCAAATTTTTAAGTAAAACAGTAGGATCCAACGAAGT1900.11397857202845865No Hit
TCGCTGGGACTTTAATGTTGATCTTGAAGAAATCAGAGAAGTCGTATCAAAG1880.11277879758605383No Hit
CTTTTGCGGGGATTCCATATTTGCCTAACGGCAATGTGGTGAAAGGTACTCA1870.1121789103648514No Hit
AGTGCTGGGAATTTGTCCTCTAGAAAATATCCAAAACAGGGTCACAAAATGA1870.1121789103648514No Hit
GCAAATGGGACCCCTCCTCCAGTGCAATGTCATCATAGACTGTCCATGACAG1860.111579023143649No Hit
GGCTTTGGGGCTTCCAGGTTACAATTGCTTGCTTTCTCATTGTCAGTAAGCA1850.11097913592244658No Hit
TGTTCAGGGTGAGAAACTGAAATCAATCACATCTCCTTGAAAATGCCTATTT1840.11037924870124416No Hit
TTGCTCGGGGCATGTATGATGGTTTTCTTCTTGAGCAACATGATTGAGAACC1830.10977936148004175No Hit
GTAGCTGGTCCCGAGTAGCTGGGACTACAGGCGCCCACCACCACGCCTGGCT1810.10857958703763691No Hit
TCGACTGGGTAGATACTGATTTATATTTCTTAAATTACATTTGCAAATGAAG1780.1067799253740297No Hit
TACGTAGGGCATCATTTCTCTGGTGAAAAAATACATTGTGGCTCCCAGTTAT1770.10618003815282727No Hit
TATTCCGGGCTGCCTCTTGCATCAGTTTGACCTGAATGTAAGACATGGAGTC1770.10618003815282727No Hit
GATTTAGGGAGCTTTATGGATTCTATTATTTAAGATTTGCAACTGATTTCAG1760.10558015093162486No Hit
GCTCGCGGGGGGACCATGGCGCAGGGGCAGCGTAAGTTTCAGGCGCACAAAC1740.10438037648922002No Hit
GCCAGTGGGTAGAGAAAAAGTATAAATGATTGACCTGACCTGACAGTATTCA1740.10438037648922002No Hit
ATGTACGGGATAAAATAATTTGCTATTTATTAAATAAAAATTCTAAATGTTT1740.10438037648922002No Hit
GTACTGGGACTACATGGGAAGAAAAGATAGAGCCTGGTTGGTAACGATTATA1740.10438037648922002No Hit
ATGGACGGGATTCAGAAATATGTGCACCCAGACTACTTTCTGCTAAATGCAG1730.10378048926801761No Hit
ATGGCTGGCTCCAAAGTAAATGAAATACTTAGGTATAAACCTAACAAAAAAA1720.1031806020468152No Hit
GAATTAGGGTGATGGACAGTCCGAACTCCCTGCTGAGGACCCCTTCAACTTC1710.10258071482561278No Hit
TCAAGGGAATATCTTGGGCAGTAATGTGATCTGGTTCATATAATTTCAAATT1690.10138094038320795No Hit
CACATGGGGTCTTTGGGCGGGGTTTCTGTTTCTTAGCATACTGTTTTGATTT1690.10138094038320795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)