FastQCFastQC Report
Sat 31 Dec 2022
SRR5848967.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848967.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences166288
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6340.38126623689021455No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5300.31872414124891757No Hit
GCTTCTGGGATACCTAGATAACATGTCTAGGTTGAGATATTTAATCCCTCAT1920.1154623304147022No Hit
GATTTAGGGAGCTTTATGGATTCTATTATTTAAGATTTGCAACTGATTTCAG1910.11486096411045896No Hit
GCAAATGGGACCCCTCCTCCAGTGCAATGTCATCATAGACTGTCCATGACAG1910.11486096411045896No Hit
GCTCGCGGGGGGACCATGGCGCAGGGGCAGCGTAAGTTTCAGGCGCACAAAC1890.11365823150197249No Hit
GTACTGGGACTACATGGGAAGAAAAGATAGAGCCTGGTTGGTAACGATTATA1820.10944866737226981No Hit
ACTACGGGGGGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGTCTCG1790.10764456845954007No Hit
ATGCATGGGGAGACTCGAGCAGTCTCTGGAACACGCTGCGGGGCTCCCGGGC1780.10704320215529682No Hit
CTTTTGCGGGGATTCCATATTTGCCTAACGGCAATGTGGTGAAAGGTACTCA1780.10704320215529682No Hit
AGCTTTGGGCACTTATTGGAATGTATCTAATGTTTTTCTCAAGATAAGACTG1740.10463773693832387No Hit
GATTCAGCCTCCAGACCAAATTTTTAAGTAAAACAGTAGGATCCAACGAAGT1720.10343500432983739No Hit
CCTAACGGGATGTGGTTCACAGTAGACATTTATTTCTCCATCTTTATTTTTT1690.10163090541710766No Hit
GGCTTTGGGGCTTCCAGGTTACAATTGCTTGCTTTCTCATTGTCAGTAAGCA1690.10163090541710766No Hit
TGTGTAGGGGAAAAACAACGTGCAGGAAATTTCCTAATGTGCGATGCACCCA1690.10163090541710766No Hit
ACACACGGGATCTGAAAGAACCAGGGGGCTGAGGCACACAGGAAACTGGAGA1690.10163090541710766No Hit
ATGTACGGGATAAAATAATTTGCTATTTATTAAATAAAAATTCTAAATGTTT1680.10102953911286443No Hit
GGCTGTGGGGCCCAGTCTACATGTACGATTTTTAACAGGCGATGTTGTTGAA1680.10102953911286443No Hit
GTAGGCGGGGGGCAGAGCCTGTGCCCTTCAGCCCTTTCACCTAACAGGCCCA1670.10042817280862118No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)