FastQCFastQC Report
Sat 31 Dec 2022
SRR5848966.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848966.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128926
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5610.4351333322991483No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5420.4203961962676264No Hit
GCACTGGGAGAGGTAGCTATTCTTGTGTCACACAAAACAAATTTTAAAGCAA2030.1574546639157346No Hit
AGCTTAGGGAATGAAGCTTTTTATTAAATAGTGGCAGAAATTCTCAAGGCTG1770.13728805671470456No Hit
GGTGATGGGGTACACGGGGATTTTATTTCTTTAAAATGCATTACTTTTTTTG1730.13418550176069993No Hit
TTAGGAGGGTCAACACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC1670.129531669329693No Hit
AACACCTGGGTAAGCACCACTAAGCAATAAGTATGTATGTAAGTATAGGAAC1640.12720475311418952No Hit
TAGAGACGGGGCGTGCTGAAGGGCGACGGCCCAGTGCAGGGCATCATCAATT1560.12099964320618029No Hit
GACTACGGGAAGACTGAATGAAAGAATAAATGCGTGCATTCCATAGGATGCA1550.12022400446767914No Hit
ACAAGAGGGAACACAAACATGCAAGAGCACAGGAACAGGCCAGCCACATCTG1520.11789708825217568No Hit
TTCTCAAGGGCAGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTA1510.11712144951367451No Hit
AAAAATGGGAGCCGGGCATAGTGGCAGGCGTCTGTGGTCCCAGCTACTCGGG1500.11634581077517335No Hit
GCGCAGGGGATTGAGCAGATACTACAGAGAATTTCCATATACCTCATATACC1490.1155701720366722No Hit
ATCGTAGGGCAAACAATTTATTTGGCTTCTGTTGCTTTATCTGTAGCATGAG1490.1155701720366722No Hit
AGACGAGGGCAAACCACAGATATGATCCAGTTAATTTCCTTCTTAAAATGTG1460.11324325582116872No Hit
AAGTTGGGGGGAGATGAAGCCACACTGGCGTGAAGCCTCGCTGCCCTGTTTG1450.11246761708266757No Hit
CGTATGGGAAACAGGATGGAAGCACTTTTAACCCAGTGAGACATTTTTGGGA1450.11246761708266757No Hit
GATTGAGGGATCATAAAAATTATAAATAATGGCCTTCTCAAAGAGGATAAAT1430.11091633960566526No Hit
GATCACGGCTTCGAGCGGGAGCATGAAAATGTGTGTTTTTTTAGGGTTTCTG1420.11014070086716411No Hit
GGCCTCATCCTCCCGCCCTGGGCTGGCCTCTGATTCCATCACGTTTGTCACT1410.10936506212866295No Hit
AAGCAAGGGCAGCCCAGCCGGGCTTCAGTTCCATATTTGCCATGTGTCTGTC1410.10936506212866295No Hit
TAGACAGGGAGCCGCGCTATCCGCTCCCGTTGCTACCGGAATGCCTCTTCAG1400.1085894233901618No Hit
AATTCCGGGAAGGATTTAAAGGGGTATTTGTAAACTTTGCCCTCCTTGAGAA1400.1085894233901618No Hit
GCAGATCTCCAAAAGAATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACA1390.10781378465166064No Hit
AAATAGGGGGATACCAACACCTATTCTGATTTTTCGGTCACCCTGAAGTTTA1390.10781378465166064No Hit
CTAGTTGGGAAGAGGATCTGGTTGATACTCTAAAGGATGTTGCCAGCTGCAT1390.10781378465166064No Hit
TTGCTAGGGAAGTGAATGCTTGATGAAAAGAAGTTTAAAACCCACCGACCTG1360.10548686843615718No Hit
GCAATTGGGGGTTGATGAGATCATGCGAGCTGGAATCTTCTCTGAAAGGTTG1360.10548686843615718No Hit
ATACAAGGGGACACCACAGGCAAGCTGGGCTTTGAGGAATTCAAGTACTTGT1360.10548686843615718No Hit
ATCATAGGGGAACACACATCTTTTATTTAAATCCATCTGAACTGTCCCAAAA1350.104711229697656No Hit
TTTTCTGGGCATGTTATATGTAATTATGATACAATTGCATACTAGATTGAAT1350.104711229697656No Hit
TCACACGGGGTCACATTAATTGATTCTCATATGTTAAACCATTCTTGCATCC1350.104711229697656No Hit
AGTGGCGGGCAGCTGAATTTCAAGGAAATACCACACGTTCATTTGAATAATC1350.104711229697656No Hit
CAGATGGGGGACACATGTCTGATGACTTAGGAAGTTATGTTTCTCTTTCGTG1350.104711229697656No Hit
TGAACTGGGCTGTCACCCAGAGCATTCTATAAAGTCAGAGTGGCAATTTGAA1340.10393559095915486No Hit
GCAGCTTCACAGTTTGACAAAAAATATACCCAAAACGCATGACTTCTTAAAC1340.10393559095915486No Hit
CTATCAGGGAACAGATGCCAATGCCAATTCCAAGGCCAGCCACAACCCTGCC1330.10315995222065372No Hit
GAATTAGGGGGAACTATGGTGCCATCCCTCAGACTTGGGAAGACCCAGGGCA1330.10315995222065372No Hit
TTGTTGGGGGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAGGT1330.10315995222065372No Hit
AAGCCTGGGTGACAGAGACAGACTCTGTCTGAAAGAAAAGAAAGAAGGAAGG1330.10315995222065372No Hit
CGCCTAGGGAATTCTGAGGAAGAAATGGAAGATGCACCAAAACAAAAGCTTT1320.10238431348215254No Hit
GTGGTTGGGATCCTATAAAACAGAATGAGTGTTGTGATGAGCTGAGCAGAGG1320.10238431348215254No Hit
CACGTCGGGATGGGCTAAATGAAAATAATCAGAATTTAAAATATTTAATGAA1320.10238431348215254No Hit
AGCTAGGGATATCAGCAATAGGCTGTTTGACTTTCTTATCATTTTTTGTGTT1310.1016086747436514No Hit
TACTAGCGGGCATGCTGGGTTTTTAATATGCTTTATCTTTTTTTTACTTACA1310.1016086747436514No Hit
AATACAGGGAGTGTTTGATCCTCCTCATTATGAACTGTTTTCACTAAGGGAC1310.1016086747436514No Hit
GTGAATGGGGATGTGGCGGAATTGGCAACACAAGAACATGAAAAGCATGAAC1300.10083303600515026No Hit
TTGCGCGGGGAGACCGCGCTGGGTTGCCGCTGCCGCTGCCGCCATCGTGCCA1300.10083303600515026No Hit
AAACTTGGGATCTTACAAAATACACATTCTTGCTTGTGTGTCTGTGTGTGTG1300.10083303600515026No Hit
GCCATAGGGCAGAATCTCAGTGGGGTGGGACCTAAGCATTTTTTTAAAGCTC1300.10083303600515026No Hit
TAAGGTGGGAGACTTTTCTTTTTAAAAATTTAACATGATTGCTTTTCTCAAT1300.10083303600515026No Hit
CCTGTTGGGGCATTAATATGTGTATCCACACACATACATGATTTTTGTTGAA1300.10083303600515026No Hit
TACTGCGGGAACTTAAGGAAAACAAGAATGTTGTCTTCTAACTCTGACATTA1290.10005739726664908No Hit
AAGCCAGGGACAGCTTCCTCACCTCTCTCAGCCTTCAAGGAATTGAAGACAG1290.10005739726664908No Hit
AGTTTAGGGCCCGGGAGGGATGGAGTGGGACAGTGTGAGATTTCATCATGAT1290.10005739726664908No Hit
TTCCGAGGGTGAATGAATAAATGTATACTGAATGAATTGTTGACAGAACTGA1290.10005739726664908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)