FastQCFastQC Report
Sat 31 Dec 2022
SRR5848965.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848965.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences128777
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5800.4503909859679912No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5040.39137423608253025No Hit
AACACCTGGGTAAGCACCACTAAGCAATAAGTATGTATGTAAGTATAGGAAC1810.14055304906932137No Hit
GCACTGGGAGAGGTAGCTATTCTTGTGTCACACAAAACAAATTTTAAAGCAA1790.1389999767039145No Hit
GCAGATCTCCAAAAGAATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACA1730.13434075960769393No Hit
CTATCAGGGAACAGATGCCAATGCCAATTCCAAGGCCAGCCACAACCCTGCC1710.13278768724228704No Hit
GGTGATGGGGTACACGGGGATTTTATTTCTTTAAAATGCATTACTTTTTTTG1700.1320111510595836No Hit
CTTTTGGGGAAATTCATAGAGATTCAGAACTTGAGGGTAAATAGCTGTCTTT1630.1265753977806596No Hit
TTTTCTGGGCATGTTATATGTAATTATGATACAATTGCATACTAGATTGAAT1620.12579886159795617No Hit
GACTACGGGAAGACTGAATGAAAGAATAAATGCGTGCATTCCATAGGATGCA1620.12579886159795617No Hit
CTTATGGGGAGAAAGTAGAAATATTACAATCAGGGAAGGTCTGAGTGCTTAC1590.12346925304984586No Hit
ATAACCGGGAGAAGCACTATGTGGTGTTCCTCAGAACAAAAAGGCTTTGATG1570.121916180684439No Hit
TAAGGTGGGAGACTTTTCTTTTTAAAAATTTAACATGATTGCTTTTCTCAAT1570.121916180684439No Hit
ATCGCCGGGCAGGAGCCACATATACTGCCATTGAGGCTCCCAAGGGAGAGTT1550.12036310831903213No Hit
CCTGTTGGGGCATTAATATGTGTATCCACACACATACATGATTTTTGTTGAA1540.1195865721363287No Hit
AAGCAAGGGCAGCCCAGCCGGGCTTCAGTTCCATATTTGCCATGTGTCTGTC1540.1195865721363287No Hit
AAGCCAGGGACAGCTTCCTCACCTCTCTCAGCCTTCAAGGAATTGAAGACAG1530.11881003595362527No Hit
GCCAGTGGGGACGGCCAGACAGGAACAAAAACCTTCCAGGCAGCGACTCCGC1520.11803349977092183No Hit
TCGTCTGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1510.1172569635882184No Hit
AAGTTGGGGGGAGATGAAGCCACACTGGCGTGAAGCCTCGCTGCCCTGTTTG1510.1172569635882184No Hit
AGCTTAGGGAATGAAGCTTTTTATTAAATAGTGGCAGAAATTCTCAAGGCTG1500.11648042740551497No Hit
GGCCTCATCCTCCCGCCCTGGGCTGGCCTCTGATTCCATCACGTTTGTCACT1490.11570389122281154No Hit
GTTAACGGGGAAAATATAAGTAAGAAGACTTTGAGTAAGAACTATGCTTTAT1480.11492735504010809No Hit
ATCGTAGGGCAAACAATTTATTTGGCTTCTGTTGCTTTATCTGTAGCATGAG1480.11492735504010809No Hit
TTAGGAGGGTCAACACTAGTGGGCCATTTTTGGTAAGCAGAACTGGCGATGC1480.11492735504010809No Hit
GTGGTTGGGATCCTATAAAACAGAATGAGTGTTGTGATGAGCTGAGCAGAGG1470.11415081885740466No Hit
GATCACGGCTTCGAGCGGGAGCATGAAAATGTGTGTTTTTTTAGGGTTTCTG1460.11337428267470123No Hit
CTAGTTGGGAAGAGGATCTGGTTGATACTCTAAAGGATGTTGCCAGCTGCAT1460.11337428267470123No Hit
AATACAGGGAGTGTTTGATCCTCCTCATTATGAACTGTTTTCACTAAGGGAC1460.11337428267470123No Hit
CATCACGGGAGTTCTGTAAAGGAAACTGTGGAATCGAATTGGCAGTGGAGTC1430.11104467412659093No Hit
TCCACTGGGAGAGAGACCAATTACGAAGAAATTACATTAACCTGAGCAAGAA1430.11104467412659093No Hit
AGTGGCGGGCAGCTGAATTTCAAGGAAATACCACACGTTCATTTGAATAATC1430.11104467412659093No Hit
CGTATGGGAAACAGGATGGAAGCACTTTTAACCCAGTGAGACATTTTTGGGA1430.11104467412659093No Hit
GCTTGGGTGGGGAAAAAAGTCTCAAAATTTAACTTCCCTTGTTGAAGAAGCT1420.1102681379438875No Hit
CTCTATGGGGCAGATGTTCCTCCCTCTCCTTCTGATGTGGCGGGATGTTTGT1400.10871506557848062No Hit
TTGCGCGGGGAGACCGCGCTGGGTTGCCGCTGCCGCTGCCGCCATCGTGCCA1400.10871506557848062No Hit
TAGAGACGGGGCGTGCTGAAGGGCGACGGCCCAGTGCAGGGCATCATCAATT1400.10871506557848062No Hit
GTAATCGGGATTGCAAATTTAAGCTTTGTCAGTATATGGAAAAGTTGAAGGG1400.10871506557848062No Hit
TTGTTGGGGGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAGGT1390.1079385293957772No Hit
GTACGAGGGGGAACAGGTAGGTCATCCTTGCGGCCTTGCGGCATGGGCAGTA1370.10638545703037033No Hit
AAACTTGGGATCTTACAAAATACACATTCTTGCTTGTGTGTCTGTGTGTGTG1370.10638545703037033No Hit
GTAAGCGGGCATCCAGGTTGTGGATAAATTGAAAGGCTTTTCAATTGCACCA1370.10638545703037033No Hit
GCGCAGGGGATTGAGCAGATACTACAGAGAATTTCCATATACCTCATATACC1360.10560892084766689No Hit
ACTCGGGGGGAAAACAACTTGGTATTTAAGTATGAAGTGGATGAAATGTTTA1360.10560892084766689No Hit
TTTGCCGGGTTAAATGGTAACATAAATATTGAAGGAGGAATACTTATGATTT1360.10560892084766689No Hit
TTCCGAGGGTGAATGAATAAATGTATACTGAATGAATTGTTGACAGAACTGA1350.10483238466496346No Hit
AATTGAGGGTCTTTTCCGTATGTGGGGATGGTTGCTACTACCTCACCGCCAA1340.10405584848226003No Hit
CAGCTTGGGCATTTAAAAAAAGGATCTGATGAGTTGCGCCAGAGTATGGTGG1340.10405584848226003No Hit
TTCTCAAGGGCAGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTA1330.1032793122995566No Hit
ATCATAGGGGAACACACATCTTTTATTTAAATCCATCTGAACTGTCCCAAAA1330.1032793122995566No Hit
AAGCCTGGGTGACAGAGACAGACTCTGTCTGAAAGAAAAGAAAGAAGGAAGG1330.1032793122995566No Hit
CTGGCTGGGGTAGGCCCAGATGCTAGATTTAACAAAGACTTAAAAATAGCTA1330.1032793122995566No Hit
GAAAATGGGATGCTATGGGCAATTTTTTTCAGCTTCACAGATTGAGACCACT1330.1032793122995566No Hit
AGACGAGGGCAAACCACAGATATGATCCAGTTAATTTCCTTCTTAAAATGTG1330.1032793122995566No Hit
CGCTTTGGGATATAGATTTGATGTATATTATTACCCAGTAAATTCTTCTTGG1320.10250277611685316No Hit
AGCATTGGGGAGAAGAGGTGACCCCGCGAAAGGAGGATGGACAGAAGAAAAA1320.10250277611685316No Hit
AATTCCGGGAAGGATTTAAAGGGGTATTTGTAAACTTTGCCCTCCTTGAGAA1320.10250277611685316No Hit
GCAGCTTCACAGTTTGACAAAAAATATACCCAAAACGCATGACTTCTTAAAC1320.10250277611685316No Hit
TCCGCCGGGACAATAGCCAAGATTTGGAAGCAACCTAAGTATCCATTGACAG1320.10250277611685316No Hit
GATTGAGGGATCATAAAAATTATAAATAATGGCCTTCTCAAAGAGGATAAAT1320.10250277611685316No Hit
GTGAATGGGGATGTGGCGGAATTGGCAACACAAGAACATGAAAAGCATGAAC1310.10172623993414973No Hit
TACCCGGGAGTGATGTTGGTGGCTGTAAGGAACAGATTGAGAAACTGCGAGA1310.10172623993414973No Hit
CTCGCAGGGACTGTGAAAGGTGTATGATCTAAACAGACAAATAGCATACTTG1310.10172623993414973No Hit
TAATTGGGGGAGAACTAAGAGACCAACAGGAACAAGTCAAAGAAGACATGGA1310.10172623993414973No Hit
TAGACAGGGAGCCGCGCTATCCGCTCCCGTTGCTACCGGAATGCCTCTTCAG1300.1009497037514463No Hit
TACTAGCGGGCATGCTGGGTTTTTAATATGCTTTATCTTTTTTTTACTTACA1300.1009497037514463No Hit
GAAGTAGGGACTTGCTCAAAACCAGAGTTGGCAACAGGTGATAAGAGCTAAA1300.1009497037514463No Hit
GGCACAGGGAGCCCTGGCAACTTTCAGCCCCTAGCTGGCATCTTGCTCACCG1290.10017316756874287No Hit
TCACACGGGGTCACATTAATTGATTCTCATATGTTAAACCATTCTTGCATCC1290.10017316756874287No Hit
TAGACTGGGTCTATAAAAATTTCTTGCAGATCCAATGCTATTTTCATAGATG1290.10017316756874287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)