FastQCFastQC Report
Sat 31 Dec 2022
SRR5848964.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848964.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127244
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5070.398447078054761No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT4720.37094086951054667No Hit
GGTGATGGGGTACACGGGGATTTTATTTCTTTAAAATGCATTACTTTTTTTG1690.1328156926849203No Hit
AGCTTAGGGAATGAAGCTTTTTATTAAATAGTGGCAGAAATTCTCAAGGCTG1670.13124390933953664No Hit
TAGACAGGGAGCCGCGCTATCCGCTCCCGTTGCTACCGGAATGCCTCTTCAG1660.13045801766684478No Hit
GTGGTTGGGATCCTATAAAACAGAATGAGTGTTGTGATGAGCTGAGCAGAGG1620.12731445097607746No Hit
AGTGGCGGGCAGCTGAATTTCAAGGAAATACCACACGTTCATTTGAATAATC1610.12652855930338563No Hit
TAGACTGGGTCTATAAAAATTTCTTGCAGATCCAATGCTATTTTCATAGATG1600.12574266763069378No Hit
AAAAATGGGAGCCGGGCATAGTGGCAGGCGTCTGTGGTCCCAGCTACTCGGG1590.12495677595800196No Hit
GTGAATGGGGATGTGGCGGAATTGGCAACACAAGAACATGAAAAGCATGAAC1580.12417088428531012No Hit
GACTACGGGAAGACTGAATGAAAGAATAAATGCGTGCATTCCATAGGATGCA1510.11866964257646725No Hit
GACTTAGGGTTTCCTGTTTTCCAGCACCCAACATAACTTGTAAGGATTCCAG1500.11788375090377542No Hit
CCTGTTGGGGCATTAATATGTGTATCCACACACATACATGATTTTTGTTGAA1500.11788375090377542No Hit
GCAGATCTCCAAAAGAATGACAGAAAGAAAGAGGGAGTGGTGGAAAGAAACA1490.11709785923108358No Hit
GATCACGGCTTCGAGCGGGAGCATGAAAATGTGTGTTTTTTTAGGGTTTCTG1490.11709785923108358No Hit
TATTTTGGGAACCCCAGGGTTGGTCAATTTCGTGCCAGCCACCGCGGTCACA1470.11552607588569991No Hit
CAGCTAGGGATTTCTTCAGCAGCCCATTAGCAACTGAGAGCCCAGGGATGTC1460.11474018421300809No Hit
GCACTGGGAGAGGTAGCTATTCTTGTGTCACACAAAACAAATTTTAAAGCAA1460.11474018421300809No Hit
GATTGAGGGATCATAAAAATTATAAATAATGGCCTTCTCAAAGAGGATAAAT1460.11474018421300809No Hit
AACACCTGGGTAAGCACCACTAAGCAATAAGTATGTATGTAAGTATAGGAAC1430.11238250919493258No Hit
GTTAACGGGGAAAATATAAGTAAGAAGACTTTGAGTAAGAACTATGCTTTAT1420.11159661752224073No Hit
ACAAGAGGGAACACAAACATGCAAGAGCACAGGAACAGGCCAGCCACATCTG1420.11159661752224073No Hit
CTGACTGGGGGGAATTTGTTAGAGCGGCCACAGGAGACTCCTAGAGAGGTCA1400.11002483417685706No Hit
TCACACGGGGTCACATTAATTGATTCTCATATGTTAAACCATTCTTGCATCC1400.11002483417685706No Hit
AGCATTGGGGAGAAGAGGTGACCCCGCGAAAGGAGGATGGACAGAAGAAAAA1390.10923894250416523No Hit
ACGCACGGGATGAACATTCCTGTACATGTCTTTTGAGAACACGCATGTGTAC1390.10923894250416523No Hit
TTGCGCGGGGAGACCGCGCTGGGTTGCCGCTGCCGCTGCCGCCATCGTGCCA1380.10845305083147339No Hit
TACTAGCGGGCATGCTGGGTTTTTAATATGCTTTATCTTTTTTTTACTTACA1380.10845305083147339No Hit
TCGTCTGGGCAACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATA1370.10766715915878156No Hit
CTTATGGGGAGAAAGTAGAAATATTACAATCAGGGAAGGTCTGAGTGCTTAC1370.10766715915878156No Hit
CGCCTAGGGAATTCTGAGGAAGAAATGGAAGATGCACCAAAACAAAAGCTTT1360.10688126748608971No Hit
TAAGGTGGGAGACTTTTCTTTTTAAAAATTTAACATGATTGCTTTTCTCAAT1360.10688126748608971No Hit
AAGCAAGGGCAGCCCAGCCGGGCTTCAGTTCCATATTTGCCATGTGTCTGTC1360.10688126748608971No Hit
ACTTTGGGGGGTTATAAAAGCCATAGTAGCCAGTCCAGTTCATTCTGCAGAT1350.10609537581339788No Hit
TTGTTGGGGGTAGGTATTAGTGCTAGTCTCAAATAGGTGCTTCCCCTGAGGT1340.10530948414070604No Hit
TTCCGAGGGTGAATGAATAAATGTATACTGAATGAATTGTTGACAGAACTGA1340.10530948414070604No Hit
GTATCTGGGCGATCTTAAACGGGGGAGGTTTTCGGGCTTGTACCGCTTGGCG1330.10452359246801422No Hit
ATACAAGGGGACACCACAGGCAAGCTGGGCTTTGAGGAATTCAAGTACTTGT1330.10452359246801422No Hit
GCCATAGGGAGACAATCCTGTGGCAAAGTAGTTCTTCTGGAATTGTTTATTT1330.10452359246801422No Hit
CGTATGGGAAACAGGATGGAAGCACTTTTAACCCAGTGAGACATTTTTGGGA1330.10452359246801422No Hit
ATAACCGGGAGAAGCACTATGTGGTGTTCCTCAGAACAAAAAGGCTTTGATG1320.10373770079532237No Hit
AAGCCAGGGACAGCTTCCTCACCTCTCTCAGCCTTCAAGGAATTGAAGACAG1320.10373770079532237No Hit
TTTTCTGGGCATGTTATATGTAATTATGATACAATTGCATACTAGATTGAAT1320.10373770079532237No Hit
TCCACTGGGAGAGAGACCAATTACGAAGAAATTACATTAACCTGAGCAAGAA1320.10373770079532237No Hit
GCGCAGGGGATTGAGCAGATACTACAGAGAATTTCCATATACCTCATATACC1310.10295180912263055No Hit
CACGTCGGGATGGGCTAAATGAAAATAATCAGAATTTAAAATATTTAATGAA1310.10295180912263055No Hit
AAGTTGGGGGGAGATGAAGCCACACTGGCGTGAAGCCTCGCTGCCCTGTTTG1300.1021659174499387No Hit
TCCATGGGGATTGCTGCTTATCAAGCAAAAAGCTTGTTTAGCCAAAGCTAGT1300.1021659174499387No Hit
CATCACGGGAGTTCTGTAAAGGAAACTGTGGAATCGAATTGGCAGTGGAGTC1300.1021659174499387No Hit
AAGCCTGGGTGACAGAGACAGACTCTGTCTGAAAGAAAAGAAAGAAGGAAGG1300.1021659174499387No Hit
GTTATAGGGGGCCACGGGATTATGTTACATTTGTCACAATTAAGGAAGAAAC1300.1021659174499387No Hit
GAAAATGGGATGCTATGGGCAATTTTTTTCAGCTTCACAGATTGAGACCACT1290.10138002577724685No Hit
ATCGTAGGGCAAACAATTTATTTGGCTTCTGTTGCTTTATCTGTAGCATGAG1290.10138002577724685No Hit
GCCATAGGGCAGAATCTCAGTGGGGTGGGACCTAAGCATTTTTTTAAAGCTC1280.10059413410455503No Hit
GCAGCTTCACAGTTTGACAAAAAATATACCCAAAACGCATGACTTCTTAAAC1280.10059413410455503No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)