FastQCFastQC Report
Sat 31 Dec 2022
SRR5848963.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848963.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131704
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3930.2983964040575837No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC3740.28397011480289136No Hit
GGCAAGGGTAATATATGAACATTTTATCATAACAGTCCATTTCTTTCAGTTT1750.13287371681953472No Hit
GCTATGGGGATGGAGACAGAATATACCAATATCTGTGGAACACACTGAAGGC1690.12831804652857923No Hit
TTAGCAGGGCTTGTGGGCAGTCATGAAAATCAATTCAGACTGTGTTGATTAG1600.12148454109214603No Hit
AAATGGGGGTTTTGGTCAGCAGTATTACTGATTTGCCTATAGAGACAGATTA1600.12148454109214603No Hit
GGCTCAGGGTAGAAAATTTTTCTCAGGAGCAAAATTATTTTTCTGTCACTTT1580.11996598432849419No Hit
GACGACGGGGAATGTAAATCATGTGGAATGTAACGGGAAATTATTTAAAGCT1530.11616959241936464No Hit
ATGAGTGGGGAATCATAGGGAATCTTTCTAGAATGTGTTTATAATTTCCTTG1520.11541031403753872No Hit
TGCATGGGGGAAACCAAGATATTGTGACACAAAATATATAGTTAAGTAGCAG1490.11313247889206098No Hit
AGAACAGGGATGTCAACATGAGCGAGTTGGTCAAGATCATCCGGCGCCGCCT1480.11237320051023507No Hit
GTCTACGGGAGTGATCTCCATTGCAAACTTTTTAATAGTGTTTTTTAATACT1460.11085464374658324No Hit
CGTACAGGGATATCAAAATCCAGACCCTATACAACATAATATCCACAATGTA1450.11009536536475734No Hit
GCAGCTGGGGATTACAGGTGTGAGCCACCCGGCCCAGCCCCTCCCTTGTGTT1450.11009536536475734No Hit
ACGCATGGGATTTTCAAAATTATCTATTACTAGGTCCACTGGTCTCCACATA1430.1085768086011055No Hit
GGCATCGGGGATATTACAAGTGTCATTTTATCTAGTAGCTGAGTTCTGTGAC1430.1085768086011055No Hit
CAGGCGGGCAGACTTTGGCTTCATTTTTAATCTTTTTGGAGATTAAGTGTCT1420.1078175302192796No Hit
AACTAAGGGATGCCCATTCTTCAGTCTTCTGTATCATAAAACTAGTCTCCTA1410.1070582518374537No Hit
GCTTCTGAGCCCTCCAAACTGTTCCAGCCTCTGTCTGTTACTCAGTTCCAAA1410.1070582518374537No Hit
AAATGAGGGGGGGAACAATCCGAACTGTAACTGAAGATTTTCCCAAGGATTC1410.1070582518374537No Hit
TTGTTGGGGAGTAGAAATGTCTCTCAAGTACCTAGTGAAACATTTTTAAAGT1400.10629897345562776No Hit
ATGGAAGGGACAGCAGCAAATCAAATGAACCCCCTGTTTACATAGAGCTTAC1400.10629897345562776No Hit
CTGAAATGGGGCTGACACACCATGAGATATTTGTCTATTTGTGTGGTGCCAG1400.10629897345562776No Hit
ATGCATGGGACGTTGTCAAAAAACAAGATAAAAGTAAAAAATCCTCTACCAG1390.10553969507380186No Hit
GCTCATGGGAATCCAATATAGTTGATAATTAAAGTGTATTTTGAATGACACA1380.10478041669197595No Hit
ACAATTGGGGAAACCAGATGAAGAAGATGAGATTTCGGAGGAATTGAAAGAA1370.10402113831015003No Hit
GAGTATGGGAAGGCCAGGTTGCGGGAGGCAGGCCTGGCACCAGTGCCCATGG1360.10326185992832412No Hit
ATTCGCGGGAGAGTTGCTATAAAATTCAGTAAAAAGCTCATAGCCAAACGGC1350.10250258154649822No Hit
TAAACTGGGATTCTACATTTAAGCTTCTGAACCTCCTATAAGTACAGAATAC1340.10174330316467228No Hit
CGTCCGGGGATCTAATTTTGATATAATTAAGCAGAACTTGCTAAAAACAAGT1340.10174330316467228No Hit
GGCATCTTTAATCCACATGAGATTCCAGAAGAAATGTGTGATTAAAATCCAA1330.10098402478284638No Hit
CACGCTGGGGACGTCAGCAGGCGGCGCGCTGTCCGCACTGCGGCACAGAGCC1330.10098402478284638No Hit
GATGTTGGGAAACCGATACATGTCAAACTGTTCAGAGAAGAAACTGCCTGAA1330.10098402478284638No Hit
TATAGGGATGTATAAATTCCTGTGTGAACTAAACTTCGTGTAAGTAATCAAA1320.10022474640102047No Hit
TTTTGAGGGAACATATAATATTTGGTCTTAAGTATTTTTGAAACATATAATT1320.10022474640102047No Hit
TTTGGCTGGGGCCTTTTAACAAACGTAGATCTCCCCATCTAGAAAATTCATT1320.10022474640102047No Hit
TGCTAAGGGAAGTGAAAGGAAGAGTCACACATCTCTCACTTTCCATCAAAAG1320.10022474640102047No Hit
GTCTGTGGGGTAGGGTGAGCCCTAAATTCAATGACTAGCTCTTAGAAAAGAA1320.10022474640102047No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)