FastQCFastQC Report
Sat 31 Dec 2022
SRR5848962.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848962.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130994
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC4240.32367894712734935No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3480.2656610226422584No Hit
GGCTCAGGGTAGAAAATTTTTCTCAGGAGCAAAATTATTTTTCTGTCACTTT1670.12748675511855503No Hit
ATGCATGGGACGTTGTCAAAAAACAAGATAAAAGTAAAAAATCCTCTACCAG1590.121379605172756No Hit
GCTTCTGAGCCCTCCAAACTGTTCCAGCCTCTGTCTGTTACTCAGTTCCAAA1580.12061621142953113No Hit
GGCATCGGGGATATTACAAGTGTCATTTTATCTAGTAGCTGAGTTCTGTGAC1570.11985281768630625No Hit
ATAGACGGGGAACCCAACTCCATTAATAAACCCCTTGGCTGGAAGAGCTCCT1550.11832603019985648No Hit
ACAATTGGGGAAACCAGATGAAGAAGATGAGATTTCGGAGGAATTGAAAGAA1530.1167992427134067No Hit
GCTATGGGGATGGAGACAGAATATACCAATATCTGTGGAACACACTGAAGGC1520.11603584897018183No Hit
AAATGAGGGGGGGAACAATCCGAACTGTAACTGAAGATTTTCCCAAGGATTC1520.11603584897018183No Hit
GGCAAGGGTAATATATGAACATTTTATCATAACAGTCCATTTCTTTCAGTTT1510.11527245522695696No Hit
TATAGGGATGTATAAATTCCTGTGTGAACTAAACTTCGTGTAAGTAATCAAA1510.11527245522695696No Hit
TTTGGCTGGGGCCTTTTAACAAACGTAGATCTCCCCATCTAGAAAATTCATT1500.11450906148373209No Hit
GTCTACGGGAGTGATCTCCATTGCAAACTTTTTAATAGTGTTTTTTAATACT1490.11374566774050719No Hit
AAGCTTGGGGCACCATCTTGTCTGTGCCTTACTGTGCACGCGGCTGGCAAAA1490.11374566774050719No Hit
CGTAGCGGGTGCTATGTTTAATGGTCACAAATTGCTAAGAGTTACATTGCAC1480.11298227399728232No Hit
GACGACGGGGAATGTAAATCATGTGGAATGTAACGGGAAATTATTTAAAGCT1470.11221888025405745No Hit
CTTATTGGGATGGCTCACTGCAGCCTCGACCTCCTGGGCTCAGGTGATCCTC1450.11069209276760768No Hit
TTAGCAGGGCTTGTGGGCAGTCATGAAAATCAATTCAGACTGTGTTGATTAG1440.1099286990243828No Hit
ATGCAAGGGGTCAGTGGGCATGGCTCTATTCCTATTCTCAGAGCATGCCAGT1430.1091653052811579No Hit
GCAGCTGGGGATTACAGGTGTGAGCCACCCGGCCCAGCCCCTCCCTTGTGTT1430.1091653052811579No Hit
AAATGGGGGTTTTGGTCAGCAGTATTACTGATTTGCCTATAGAGACAGATTA1430.1091653052811579No Hit
GTACTCGGGACTGCTTCTCTAGTGAGTAAAATTGGCCTGGATACAGAAGGAG1420.10840191153793304No Hit
ATATGTGGGGTCTCTATAACCTAACAATGAGGTCAGGGACTTTGTTTTGTAT1410.10763851779470815No Hit
TACCAAGGGGGGAGGAATGTGTGAGATAAACATTAGCGTCAGCACTACAAGG1400.10687512405148328No Hit
GAAACAGGGTTCATTCATGTAAAAAATTCCTCATATAAGAAAGGTTCTTGGT1380.1053483365650335No Hit
CTGAAATGGGGCTGACACACCATGAGATATTTGTCTATTTGTGTGGTGCCAG1360.10382154907858374No Hit
TCGTGTGGGGAATTGGAGCAATGTTGGCTTATACACCTCTGGATGAGAAGAG1360.10382154907858374No Hit
GAGTATGGGAAGGCCAGGTTGCGGGAGGCAGGCCTGGCACCAGTGCCCATGG1340.10229476159213399No Hit
TGCTCTGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC1340.10229476159213399No Hit
TCGCATTGGGGAAGAGAACTGCAACTGAATGACTAATCAGATGATGGCCATT1340.10229476159213399No Hit
GTCTAGGGGATTACTAGAAACTGGTAATTTGAAAAGATCAATAAATATGACA1340.10229476159213399No Hit
TTATGCCGGGTACATTTATTTCCTAATTTATACATGATTTTTGGTGTTAATA1330.10153136784890912No Hit
CGTCCGGGGATCTAATTTTGATATAATTAAGCAGAACTTGCTAAAAACAAGT1330.10153136784890912No Hit
CGTACAGGGATATCAAAATCCAGACCCTATACAACATAATATCCACAATGTA1320.10076797410568422No Hit
TAACGACGGGATCTGGCATGGTTTTAGGTGAGTGGTCACTATCCAGGTTATC1320.10076797410568422No Hit
CTTGATGGGGGAACTTTTCCCTAATAGCCGCCTCTACCTTTTTTCAAAGTCT1320.10076797410568422No Hit
TGCGAGGGGATCTCTAACAGGGGATTTTGTTATTTTGTTTCTTCTACTAATT1320.10076797410568422No Hit
TAGGTTGGGATCTCTAATTTTGAGAAAAGTAACCTTAGCAGATAAGGAAAGA1320.10076797410568422No Hit
ATACATGGGACGATCAAAACTACTGAATTAGTAGAAATAATGATGTCTAAAG1320.10076797410568422No Hit
AGCATAGGGGGTGGCCCGGGCGTCCGCTCCTCAGCGGATGTGGCAGCCCCGA1310.10000458036245935No Hit
TTCACAGGGATACATGGAATTGATATTGCTTATTCTGACTAAGTGAAAAGTG1310.10000458036245935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)