FastQCFastQC Report
Sat 31 Dec 2022
SRR5848961.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848961.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130847
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC3990.3049362996476801No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT2930.22392565362599065No Hit
TTAGCAGGGCTTGTGGGCAGTCATGAAAATCAATTCAGACTGTGTTGATTAG1670.12762998005303905No Hit
GACGACGGGGAATGTAAATCATGTGGAATGTAACGGGAAATTATTTAAAGCT1620.12380872316522351No Hit
GCTATGGGGATGGAGACAGAATATACCAATATCTGTGGAACACACTGAAGGC1520.11616620938959242No Hit
ATGCATGGGACGTTGTCAAAAAACAAGATAAAAGTAAAAAATCCTCTACCAG1480.11310920387934No Hit
AAGCTTGGGGCACCATCTTGTCTGTGCCTTACTGTGCACGCGGCTGGCAAAA1460.11158070112421378No Hit
AAAATTGGGCTCCAGCCATTGCACCCCAAAGCAGTGCCAGCCGTCCGACACC1460.11158070112421378No Hit
GTGATGGGAATACAACTAGATTCTTTATTTTGCAACATGATTGGTTCAGTAA1430.10928794699152444No Hit
GACACTGGGGGGATATTCGCTTGCTTTGTGAAAAACATGAAAATGTTAGCAG1420.10852369561396136No Hit
GGCTCAGGGTAGAAAATTTTTCTCAGGAGCAAAATTATTTTTCTGTCACTTT1410.10775944423639823No Hit
TATAGGGATGTATAAATTCCTGTGTGAACTAAACTTCGTGTAAGTAATCAAA1410.10775944423639823No Hit
GCACTTGGAACCCTATTTCTAAATAAGGTAACATTTTGAGGTTCTGGGTGGA1400.10699519285883513No Hit
GCAAACGGGATTTACATTAGCAGTCCCTAAAATGAAATACTTAGGTATAAAT1400.10699519285883513No Hit
CGTACAGGGATATCAAAATCCAGACCCTATACAACATAATATCCACAATGTA1390.10623094148127202No Hit
CGTCCGGGGATCTAATTTTGATATAATTAAGCAGAACTTGCTAAAAACAAGT1390.10623094148127202No Hit
TAACATGGGATAAATAAATGACAAAAGAAAGAGAAAAAGGCCAAAAAGTCTA1390.10623094148127202No Hit
GTGGATGGGGTCTCTTCATTCTATTGATTTTTTCCTTTGGTATACAGAAGCT1390.10623094148127202No Hit
CGTAGCGGGTGCTATGTTTAATGGTCACAAATTGCTAAGAGTTACATTGCAC1380.1054666901037089No Hit
GGTCCGGGGTTATGGAATGCTGATGCATTCTTAGTCTAGGAAACTCACAGTT1380.1054666901037089No Hit
TGCATGGGGGAAACCAAGATATTGTGACACAAAATATATAGTTAAGTAGCAG1370.1047024387261458No Hit
CACGCTGGGGACGTCAGCAGGCGGCGCGCTGTCCGCACTGCGGCACAGAGCC1350.10317393597101959No Hit
AATAACGGGGGCCTCTGCGCGCGGGAAGATGGCGGAACAGGCTACCAAGTCC1350.10317393597101959No Hit
CTTGATGGGGGAACTTTTCCCTAATAGCCGCCTCTACCTTTTTTCAAAGTCT1350.10317393597101959No Hit
GGCATCGGGGATATTACAAGTGTCATTTTATCTAGTAGCTGAGTTCTGTGAC1330.10164543321589338No Hit
ACAATCGGGACTGGAATTACAAAACCTATTTTTCCTTTAAATTTCAGCTTTG1320.10088118183833027No Hit
TTTTCCGGGGGATGGAGGGAGAGAAGGACAGGGCTGAAAAACTTCCTATTGT1320.10088118183833027No Hit
GGCAAGGGTAATATATGAACATTTTATCATAACAGTCCATTTCTTTCAGTTT1310.10011693046076717No Hit
ACAATTGGGGAAACCAGATGAAGAAGATGAGATTTCGGAGGAATTGAAAGAA1310.10011693046076717No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)