FastQCFastQC Report
Sat 31 Dec 2022
SRR5848954.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848954.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96674
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6220.6433994662473882No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5760.5958168690651054No Hit
ATTTCCGGGAATGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGC1790.18515836729627408No Hit
CATTAAGGGCTAATATTTCAGCTGTTGAAACAGAAGAAGTTGAGCCTGATGA1730.1789519415768459No Hit
CATACAGGGATAGGGACTAATATTTCTTTTTCTTTTTTTTAAAAAAAACATT1670.1727455158574177No Hit
CGCACTGGGACATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGT1620.1675734944245609No Hit
TAAAATGGGACTGTGCCTGAGCTCTGGAGTGCCAAGGCCTACCCATCGCTCA1560.1613670687051327No Hit
TGCACTGGGTTATATTCTCACTATCTGTAAATGAACATTTCTGTTGCTCCAC1470.15205743012599043No Hit
TAACTAGGGCATCGAAAAACTTGAGAGTAAGGTATATTTGGGGAGAATATGG1470.15205743012599043No Hit
TCTCAGGGGGCTTCCCAACATGGCGCAGTCTATTAACATCACGGAGCTGAAT1460.15102302583941907No Hit
CTGAATGGGATAATAGATGTACAAGGAAATGCATTTTCTGCCTCCACCCCCT1440.14895421726627636No Hit
ACACGCGGGATAGAATGTTTGTATGCTCTAAGACTAACTTTTAGATTTTCTT1440.14895421726627636No Hit
ATTTAGGGGGAGACCGGAAGTGAGTGATCGAAAGCATGGCGTCGGTGGTGTT1420.14688540869313363No Hit
AAACTCGGGGAAAGGGGAATTATATGCTTAAGAAAAAGTAGGACACGGTTGA1410.14585100440656226No Hit
GTATCTGGGATTTCCGAGATGTGGAGGCAGAGCCACCCATCCATCCTCCCGT1400.1448166001199909No Hit
CTAGGTAGGCAACTAAGATTGGTAGAGCAAGGGAAAAATCTCAGCTGCTTGT1380.14274779154684816No Hit
TTTGTCGGGCTGCTTCTGAAATAGAGAACTGCTATGATCTTCTGGTCAGAAA1380.14274779154684816No Hit
TTCTAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1370.14171338726027682No Hit
GTACAGGGGAGATTAAAATTTATATTACACATCCTTTCATGCCCCTCAGAAA1370.14171338726027682No Hit
TGCGTTAGGGAAGCTTTGATATGTGAATATACCATCACCACAATCAAAATAA1370.14171338726027682No Hit
TGCCTGGCTAGTTTTTTTTGTATTTTTTAGTGGAGACGGGGTTTCGCCGTGT1370.14171338726027682No Hit
CCACATGGGGTTTGGGAGAAAGTAAGAGAAAAGAACTAGGGTCTCTGCCTGG1360.14067898297370543No Hit
GCACCAGGGGAATATCAAGAGGAGGAGTTGTTGGTAATGTGTCATTGTTTGT1360.14067898297370543No Hit
CTCTTAGGGCAAATGAGGTAGAGCTCACCTGTGCTCACCAGCTCCGTCAGGG1340.13861017440056272No Hit
CCGATTGGGATATTTCAAGAAAAAGAAATTATGTTAAATAGCCCTGTTTTAA1340.13861017440056272No Hit
TGACACGGGGAATGAATCTGAGCTTGGTTTCCTTTATTGCTTCCTCTGCAAT1330.13757577011399136No Hit
GACTTAGGGGGTTTATTCTCAGGTAATAATTTGGGAAGTGTAAGAAAATATG1310.13550696154084862No Hit
AAACCAGGCTGGCAATGGGCTCCCTACCCACACTGGCTTCCTGCCTCCCACC1310.13550696154084862No Hit
GCTTACGGGATTCCTTCCTTCAATTTGACCCAGCGCCTCGTCGTGGTGAGCC1260.1303349401079918No Hit
ATGTATGGGAGTGTACAATTTGGTAGTTTTTTATATATTCACAAGGTTGTAC1260.1303349401079918No Hit
TACACCGGGGGTTCTAAAAATTGGTAATATTCAATAGTGGTTTTTTGTTCAG1250.12930053582142045No Hit
TTTATGGGGATTGGACCAAATTTGGTGTTTGTTTTCACCTTGACGCTCTTCT1240.1282661315348491No Hit
CGCAACGGGATCACTACCTTTTATTTTGGCCACTCTGATAGGTGTGCAGGTG1240.1282661315348491No Hit
CAAACAGGGATGGAGGTGGCGAACGCTGTGCGCGCCTCCGACCCTGCCGCCT1240.1282661315348491No Hit
TCGTTAGGGACAGATAAATGAATTTTCACAAACTCAGTGCACACATGTGTTT1230.12723172724827772No Hit
GCCGCCGGGTCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGA1230.12723172724827772No Hit
GAGCATGGGACGTGGGTGGTCCTGGGGCACTCAGAGAGAAGGCATGTCTTTG1230.12723172724827772No Hit
TTTACCGGGATCAATAAAATACAAATAATCCAATTAAAAATAGGCAAAGGAT1230.12723172724827772No Hit
TCGAAGTGGGGGCCATCTAAAAATGAGAATTATAATTATATGAATTATAATT1230.12723172724827772No Hit
GAAAAAGGGAAGAGTGAAGCAGTTAGAGTCTGCAGCCCTGGAATGTCTGGGT1220.12619732296170635No Hit
TATCAAGGGAATCCGCCTCCAGGTAACTCGCTAATTTCCAGTGTTAAGGTGT1220.12619732296170635No Hit
ATGCTGGGGGGAAGCGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTAA1220.12619732296170635No Hit
TTGCGTGGGATACAAACAGACAAAAAATAAAACAAAACTTGAGTTCTATTTA1210.125162918675135No Hit
CAAGCTGGGACATGAAATAAAGGTATTATCCTCTACTCATAAAGAAATACCA1210.125162918675135No Hit
CAGGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1210.125162918675135No Hit
CTTAGCGGGGTTTACATACTGAATTCTGTAATATGAACTAGGAACGCATTTA1210.125162918675135No Hit
GCGTTTGGGAGGGTGTGTTCACATATATCTCAGTTGAATGTCACAAGAATCC1200.12412851438856362No Hit
GGCAGGGGATATTCTAAATCTACCACCTTAGAAAATTCTAAAAAACACAAGA1200.12412851438856362No Hit
GCTCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGG1200.12412851438856362No Hit
ACTGCTGGGAAGAGTGGACAGCGGGGATCTTCCAAGTCTGGAAAGTTGAAAG1200.12412851438856362No Hit
CAGGCTCGGGATGAAAAAGTTTTTGCTGACACAGAACAAACCTGAAAGTAGT1200.12412851438856362No Hit
CAGTACGGGATGAAATGAGCAAAAATTATTGAAAGTCAAAACTAACTAAAAT1200.12412851438856362No Hit
AATGTTGGGCGATCTTAGACGGGGAAAGTACATAAATGCCTTTTTACAGCTT1190.12309411010199225No Hit
AACACGGGGGATTTACATTTTGATAGCCATTGATGTATGCATCTCTTGGCTG1190.12309411010199225No Hit
ATAGGCGGGATGACTTCTACTCTTCCTTAGATATCCTCCAAATGACAAAGAG1190.12309411010199225No Hit
GCACGCGGGGGGCTCAAACGTAGGCGTCTGTGTTTCGCAAAAGTTGGAGACG1190.12309411010199225No Hit
AGACATGGGTAAGACCTAATTTGATCTTCATGACAACTGCATAAAATAGTTA1180.1220597058154209No Hit
ACTATCGGGAGTGATGGAAGGCGGTTATAATGGATGGTGGTGTGTACAAGAT1180.1220597058154209No Hit
TAGTCAGGGATAGGGCAGAAAGGCCATTCCCTGAGGAAGATCCTGGACCTGA1170.12102530152884954No Hit
CCTGATGGGAGACGGAGTTTGAGCGCGTCTTCTGAGGGCCGGCGCTTGCCAC1170.12102530152884954No Hit
CTAATGGGAGTTGCTGGTTTCATAACCTTGAACAAGTTACTTAACTTCTCTG1160.11999089724227818No Hit
CATGTTGGGGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCATCCGC1160.11999089724227818No Hit
CATAAGGGGGTTGGCCAGGCTGGTCTCGAACTCTTCACCTCAAGTGATTCAC1160.11999089724227818No Hit
TAACTGGGGGTGCTCTAAAACTCTTCTACTTGGTGAGCATCTTGATTGGTTA1160.11999089724227818No Hit
TCCACAGGGTGGAAACAATATTCTCACATTAGATACTAAATGGTTTATACTG1160.11999089724227818No Hit
GCAACGGGGCCAAAAAAGAATCTGCTTAGATTGCTAAGGTTAGAGGTAAAAA1150.1189564929557068No Hit
TCAATGGGGCGGTTTTGGTTATGCAGCCCTCGTGACATTTGGAAGCATTTTT1150.1189564929557068No Hit
GATAATGGGGCACTGTTTCTTTTTGCATATAAGCATAAGTAACTTCTCTCAG1150.1189564929557068No Hit
ATGCGCGGGGCTTGGGACAGACCATAAATGATGGAAAATGTCAACACAAAAA1140.11792208866913544No Hit
ATATATGGGCAATTACCTAACCACTAAACAGAAAGGCCACCTTCACTCTTTG1140.11792208866913544No Hit
GAGAGGGATAATTATAAATTGGTATTTGCTAGGTAGATAAAACTTAAGACAT1130.11688768438256408No Hit
GCTGCTGGGATTGAGAAGTTCCATCAGAGTTGGAGATTCAGAGGAAGGAAGA1130.11688768438256408No Hit
TTCTGGTGGGGGAATTTGAAGCCCTGACAAAGAGAAGGCAAAATCAGTTTGT1130.11688768438256408No Hit
GACTACGGGGAAGTCCCTGTTATCGAATACAGATACTTTAACAAGCAGACAA1130.11688768438256408No Hit
TCATTTGGGGCATATGGGAAGTAACATTGCACTAATATGGAAATCACTGCCA1130.11688768438256408No Hit
ACTAGTGGGTTGCTGGGATTACAGGCATGAGCCACTGCACCCAGCCTATCGT1130.11688768438256408No Hit
CAGGGGATACTATGCATTTAGAAATAACTCAAACAACTCAAGTTTATCACTA1120.11585328009599272No Hit
CACAAAGGGATAACACAGCTGAATTTGGACTGGGCACATTTCACATGTGGCT1120.11585328009599272No Hit
TAGCTGGGGGATCTTCTGTAGGGGTGTGTTCCAAGTTTCCAAACAACAGAAA1120.11585328009599272No Hit
TTAACAGGGAGATAGCAAAGTGGGGAATAAAAGTCTAACTACAAGCTTATTA1120.11585328009599272No Hit
AAGCTGGGAAGCAGATCTGGGTTGCAAACCTGCCTCTACTGCTTTACTATTC1120.11585328009599272No Hit
TATGCAGGGAGGAAATGAATATATGTGAGTTCTAGCATTTCTCCCCACCCCC1110.11481887580942135No Hit
TTCAAAGGGCGGCTGCCCCGTCTGGGAAGTGAGGAGTGCCTCTGCCCGGCCG1110.11481887580942135No Hit
AGACGGGGGTTGAGAATTAGAGACACAAGAGAGGCTGTGGATGGCCTATTAA1110.11481887580942135No Hit
GCTATGGGGCAAATCAATAAACGTAATCCATCACATAAACAGAACCAAAGAC1110.11481887580942135No Hit
CGGTATGGGAACTTAAAAGATGCCACCTCTAAGGTAGCGCTGGTATATGGTC1110.11481887580942135No Hit
AGAGCGGGTAGCTGGGACTACAGGCAAACACAAACACACAGCTAATTTTTCT1110.11481887580942135No Hit
GTTACTGGGATACATACTTTGATTCTTTAGTTACATAATTGATAACAAAGTG1110.11481887580942135No Hit
TCGAAAGGGACAGTAATAGATTATGAAGGATGAATTATTTGAAAGCTATTTT1100.11378447152285No Hit
TAACAAAGGGGATGAAAATGTTTTTGTGAAGTACTTGTAACTAAATTCCTAC1100.11378447152285No Hit
TTGTGAGGGCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATC1100.11378447152285No Hit
TCGCACGGGACTTTCTTCACTGTCCCTATTCCTAGACCTGGGGCTTGAGCTG1100.11378447152285No Hit
CGCCAGGGATTGAGTTGAGAAACTGAGTTCTGCTGTGAACTTCAGGATCTTT1090.11275006723627863No Hit
TCGTTTGGGATTCTCCTGAGGCATTAGATAGTTTTTCTATTATAAGTGACAG1090.11275006723627863No Hit
GCAAAAGGGTAAAAGTCAGCATTTAATATACAGTGTTTGGTTTGGAAACCAT1090.11275006723627863No Hit
GTGAACAGGGTAATGGATAAAGGTATACCTCAGACTTCACTGTGCTCACAAA1080.11171566294970725No Hit
AGCCGTGGGTCCTCCCTCCTACCCTGGCACGTGGAATAGGGCTTACTCACCC1080.11171566294970725No Hit
TAGCATAGGGGTAACCTCAAGCATAGGCAACATTTTCCTATTTCATGTTATT1080.11171566294970725No Hit
TACACTGGGGAGACATAAAGTGGATATTGGCTTCAGGGGTGTTCAACATTAA1080.11171566294970725No Hit
TACTATGGGACCTCTTTCAGGTTTTAATTCAGTTACCACCTTCTTGGTAAAA1080.11171566294970725No Hit
CTATTGTAGGGAGTGCTGTTTCACTGTGGTCAGAGACTGTGCTCTGCGTGGG1070.1106812586631359No Hit
AAACACGGGGGGTTCGGTATTTTCTAACTGAAACTTGGCACTGCCATCAGTT1070.1106812586631359No Hit
AGTGGTGGGACTGCTTACAAAACAGGGTCACCAGTGCATTATGTGGCGTGTT1070.1106812586631359No Hit
GGTGGTGGGACGGATGAGAGACTGGCTCAAGAATATCCTCATGCAGCTTTAT1060.10964685437656453No Hit
GAATGCGGGATTCTTGCAGATTACATGTTTTTACAGTGGCCTGCTATTGAGG1060.10964685437656453No Hit
TTAAAAGGGGGATATTGTGAGTTAATACTTTAGAAAAAAATCCATCAACTCA1060.10964685437656453No Hit
CCGACAGGGCAAGTAACTATCACAAGAACCACTGTCCCAAGATCCAAAGTCA1060.10964685437656453No Hit
TGTGATGGGGGATGGAGATCCCTGGTTGTTAGAAGGAGGAAAAGAGATTTTG1060.10964685437656453No Hit
AGCGCTGGGCAACTTTATTATCTTACACTTCTGGAGTGCGGATTCCTAAAAC1060.10964685437656453No Hit
TTATGAGGGATATTAAGACTGTAGCTGAATTGCACATGAAATCAGATTGCCA1060.10964685437656453No Hit
ATACTTGGGAGAGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAG1050.10861245008999318No Hit
ACATAAGGGGCTATGTTTCAGTGGAAGAAATTCTTTGAAGTAGATGTGAGTG1050.10861245008999318No Hit
AAAGCTGGGATCTGACATGGGGAGTTGTAATTGTTCATATAGGAAGGAAATC1050.10861245008999318No Hit
CGCGACGGGACATTGGAAAGTTCTGCAATAAGATTCTAGTTCTCTCTTTTCT1050.10861245008999318No Hit
TCAGTGGGTAGGGGAGGGGAAAAGGTTGATATAATAATGCAGGGTTGCTCCT1050.10861245008999318No Hit
TGCCTTGGGCACAATGTGAGACAGCAACCATTTCCTTCCCAGGATATCATAG1040.10757804580342181No Hit
ATAATGGGGGACGCTCTTCCGATCTGGACCTGGGGTGCATTCAAGACAGATA1040.10757804580342181No Hit
TATTCTGGGAGAGTACCGAATGACATCAGGATCCTGGACAGAAACCCATGCA1040.10757804580342181No Hit
GTAGAAGGGATGACAATTTTATTTAGGTATATCTCTAAGTTTTTCTTTTATT1040.10757804580342181No Hit
GCGCTAGGGACGGTTAAAACAAAGAAAATGTTGTCAACGTTGACGCCAACAT1040.10757804580342181No Hit
ACTTCAGGGGGGCTTTTAAACAAAGTTACCATGCCAGAAGTGTTCTTGGCAT1040.10757804580342181No Hit
TCAACAGGGAAGAGCCAGCAGGAGCAGCGTGGTCATTGCTGGGTGCTATTAC1030.10654364151685045No Hit
CAGATTGGGAGTTTGCATTTTTTGGTGTTCATTTTCATTCTGTTGATTTCTC1030.10654364151685045No Hit
GCTCTCGGGGTTGACAGAGGATTATACATGACAGTCACAGTGATAGCATGAA1030.10654364151685045No Hit
GTACTAGGGAAGATCGATCCCCTAAAACCAGCTTGATTTCCTGTGTGCAGAA1030.10654364151685045No Hit
TATGAGGGTTGTTTGATGAGACTGACATTGCCTAATTTCAAGCTTTTTGAAT1030.10654364151685045No Hit
ATGTTAGGGATGTTTTTCTAAGGAAACTTTCCACAGAATTTTAAACAACTGA1030.10654364151685045No Hit
TGACATCGGGGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATG1030.10654364151685045No Hit
GTAAATGGGATTTGAATCTTTGTACCTCCATACAAGTGTTAGCCTGCCAGGC1020.1055092372302791No Hit
GAGTTCCGGGATTTATGCAAGTCATGCTTTCATTCTACATGTAGTTAAAACT1020.1055092372302791No Hit
TCGGTGGGTATATACAGATACTTTCATGGTCATTTTGTATGTATGTTAAAGT1020.1055092372302791No Hit
CATAGCGGGAACACAGTTCAGAGAGATTTTCATCGGGTGCATTCTCTCTGCT1020.1055092372302791No Hit
CACAAAGGGGGCCCTGTTATGTGCCTTACTGTGGATCAGATTTCCAGTGGAC1020.1055092372302791No Hit
GATCTTGGGCAAAAAATCCTTGGAGTGCTTGCCTTTCCACGTGACTTTTAAA1020.1055092372302791No Hit
CTCCTTGGGATTGAACAAATGACAAGGCACAAATACTTTCAGAATTGTATTT1020.1055092372302791No Hit
GATATTGGGGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACT1020.1055092372302791No Hit
TCAATGGGTTTTGGAGTCGTAGGAGTTACATCTGCTAACATGCTTATTTTCA1020.1055092372302791No Hit
TTTACCGGGATGATCTGAAAACCCAGTGGATGGCTGAAGGTGTTAAATGTGC1010.10447483294370771No Hit
TACAGCGGGAGATGCTAGATTTGAAGAAAACCTGGGTTTTATTGCATAGATT1010.10447483294370771No Hit
AAACTAGGGCTCTTCCGATCTGGCTGTGGGATAGGATAGAGAAAAATCTGCT1010.10447483294370771No Hit
TCTAGGGGGATCTGCCAGTGCTTTCTTCAAGTCACCCTTGCCCCGATTCCTC1000.10344042865713635No Hit
CCTCGCGGGACTGTATACAAAACTGTTCTCTTGTTCTGCCTTTTAAAATGTT990.102406024370565No Hit
CGGTCTGGGGGGACGGAGTCTCACTCTGTCACCCAGGCTGGATGCAGTGGTG990.102406024370565No Hit
ATTCCTGGGCACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGT990.102406024370565No Hit
GGCCATGGGTATATAAAAATAGTAGAGTATAATGAATTTTCTTCTGTTGATT990.102406024370565No Hit
TCATAAGGGATGGGATTTAATGGGAAAAATATGTAAAAACTGTCACTAGTCA990.102406024370565No Hit
AATGTAGGGCACCGAGGTTCCAACAGCAAGTGTGGCCATTGAGGGGGCATCC980.10137162008399363No Hit
AAGTACCGGGGGATTATTATAAGTTTTCAATCCTGGTATCTGTGCTTGATTT980.10137162008399363No Hit
GCTTTTGGGATTTATGCATTTGTAGGTACTATTTTTCTAGTAAGAAGTGGAA980.10137162008399363No Hit
CCATTAGGGTAGTGAAGCATTGATGAGGAGAGCAGTGTCTTTGGTAACAGAT980.10137162008399363No Hit
TCTCCGGGTCAGGAGTGCCTCACGTAGATAGATTGAGGTTTTATAATAATCA980.10137162008399363No Hit
TCATCAGGGATATGTGACTTTTTCATTTCTTTTAACAGGAAAAAATTAAAGA980.10137162008399363No Hit
AAGCAACGGATGGCAGAATATTTATACATGAAAAAATAATTTTGCAAATATT980.10137162008399363No Hit
TTTGCTGGGAAATTCGATAGGGAAACAGCCTTGACCTGTACTTTACTAAATG980.10137162008399363No Hit
TAAATGGGTGAATTCAATGATGTGAAGACCTTGCTCAGAGAATGGATAACTA980.10137162008399363No Hit
GGTCACGGGTAAGGTGACTCAAGATGATACACCGAGAGAAAAATGCAAAATA970.10033721579742226No Hit
GCGTTGGGGCAAGTCATGGACCACATAATCTTTTCCCCCACTGGCAAAGTGA970.10033721579742226No Hit
GATTTCGGGATTTTTAGAGATTAATTTGTGTAGATTGTGTAAATTCCTGTTG970.10033721579742226No Hit
GATTATGGGCGATCTAGACGCGGGAATAGGAACTTGCACAGCATCCATCATA970.10033721579742226No Hit
CTACTGGGGCTTTTCAAAAAGCAAACTCTTAGTTTTTTTGTTCTTTCGGTTG970.10033721579742226No Hit
TTCGCCGGGGGTGGTTGGTGTGCGGGTTTCGGTTGGAGGACTCGTTGGGGAG970.10033721579742226No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)