FastQCFastQC Report
Sat 31 Dec 2022
SRR5848952.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848952.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96975
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5810.5991234854343903No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT4980.5135344160866202No Hit
AAACTCGGGGAAAGGGGAATTATATGCTTAAGAAAAAGTAGGACACGGTTGA1720.17736530033513792No Hit
CATACAGGGATAGGGACTAATATTTCTTTTTCTTTTTTTTAAAAAAAACATT1690.174271719515339No Hit
CGCACTGGGACATGTAGCAGTAAAAATAGTTAAAAATGTGGATAGATACTGT1680.17324052590873937No Hit
TTTGTCGGGCTGCTTCTGAAATAGAGAACTGCTATGATCTTCTGGTCAGAAA1640.16911575148234081No Hit
TGCACTGGGTTATATTCTCACTATCTGTAAATGAACATTTCTGTTGCTCCAC1610.16602217066254188No Hit
GCCGCCGGGTCTCTTGGGCAGAATCCCACAGAAGCAGAGTTACAGGACATGA1560.1608662026295437No Hit
TTATGAGGGATATTAAGACTGTAGCTGAATTGCACATGAAATCAGATTGCCA1540.15880381541634442No Hit
CATTAAGGGCTAATATTTCAGCTGTTGAAACAGAAGAAGTTGAGCCTGATGA1530.15777262180974477No Hit
GATCTTGGGCAAAAAATCCTTGGAGTGCTTGCCTTTCCACGTGACTTTTAAA1520.15674142820314513No Hit
GAGCATGGGACGTGGGTGGTCCTGGGGCACTCAGAGAGAAGGCATGTCTTTG1450.14952307295694767No Hit
GTATCTGGGATTTCCGAGATGTGGAGGCAGAGCCACCCATCCATCCTCCCGT1440.14849187935034805No Hit
CTAGGTAGGCAACTAAGATTGGTAGAGCAAGGGAAAAATCTCAGCTGCTTGT1430.1474606857437484No Hit
GAAAAAGGGAAGAGTGAAGCAGTTAGAGTCTGCAGCCCTGGAATGTCTGGGT1410.1453982985305491No Hit
GATATTGGGGACACCATTCTCTCTCCATTTACTTCTGGTGGTTACCCTGACT1380.14230471771075018No Hit
TGACACGGGGAATGAATCTGAGCTTGGTTTCCTTTATTGCTTCCTCTGCAAT1370.14127352410415056No Hit
CATAAGGGGGTTGGCCAGGCTGGTCTCGAACTCTTCACCTCAAGTGATTCAC1350.13921113689095127No Hit
CTGAATGGGATAATAGATGTACAAGGAAATGCATTTTCTGCCTCCACCCCCT1350.13921113689095127No Hit
GTACAGGGGAGATTAAAATTTATATTACACATCCTTTCATGCCCCTCAGAAA1350.13921113689095127No Hit
AAACCAGGCTGGCAATGGGCTCCCTACCCACACTGGCTTCCTGCCTCCCACC1350.13921113689095127No Hit
GCGTTTGGGAGGGTGTGTTCACATATATCTCAGTTGAATGTCACAAGAATCC1330.137148749677752No Hit
ATTTCCGGGAATGGTAGGCACTGTGATCATGATGAAAATGAATGAGGATGGC1330.137148749677752No Hit
TCGTTAGGGACAGATAAATGAATTTTCACAAACTCAGTGCACACATGTGTTT1320.13611755607115236No Hit
CAGATTGGGAGTTTGCATTTTTTGGTGTTCATTTTCATTCTGTTGATTTCTC1310.13508636246455272No Hit
GACTACGGGGAAGTCCCTGTTATCGAATACAGATACTTTAACAAGCAGACAA1310.13508636246455272No Hit
GCTATGGGGCAAATCAATAAACGTAATCCATCACATAAACAGAACCAAAGAC1310.13508636246455272No Hit
TCGAAGTGGGGGCCATCTAAAAATGAGAATTATAATTATATGAATTATAATT1300.13405516885795307No Hit
TCAATGGGTTTTGGAGTCGTAGGAGTTACATCTGCTAACATGCTTATTTTCA1300.13405516885795307No Hit
CAAGCTGGGACATGAAATAAAGGTATTATCCTCTACTCATAAAGAAATACCA1290.13302397525135343No Hit
TTTACCGGGATCAATAAAATACAAATAATCCAATTAAAAATAGGCAAAGGAT1290.13302397525135343No Hit
TCATTTGGGGCATATGGGAAGTAACATTGCACTAATATGGAAATCACTGCCA1290.13302397525135343No Hit
TCAACAGGGAAGAGCCAGCAGGAGCAGCGTGGTCATTGCTGGGTGCTATTAC1280.1319927816447538No Hit
GCAACGGGGCCAAAAAAGAATCTGCTTAGATTGCTAAGGTTAGAGGTAAAAA1280.1319927816447538No Hit
ACACGCGGGATAGAATGTTTGTATGCTCTAAGACTAACTTTTAGATTTTCTT1280.1319927816447538No Hit
ATTTAGGGGGAGACCGGAAGTGAGTGATCGAAAGCATGGCGTCGGTGGTGTT1270.13096158803815416No Hit
TTGCGTGGGATACAAACAGACAAAAAATAAAACAAAACTTGAGTTCTATTTA1270.13096158803815416No Hit
CAGGGGATACTATGCATTTAGAAATAACTCAAACAACTCAAGTTTATCACTA1270.13096158803815416No Hit
TACACCGGGGGTTCTAAAAATTGGTAATATTCAATAGTGGTTTTTTGTTCAG1270.13096158803815416No Hit
TGACATCGGGGAAAAACATACATTATCAACATGTTTTTGATATTTGATAATG1270.13096158803815416No Hit
TCAATGGGGCGGTTTTGGTTATGCAGCCCTCGTGACATTTGGAAGCATTTTT1250.1288992008249549No Hit
TGCCTGGCTAGTTTTTTTTGTATTTTTTAGTGGAGACGGGGTTTCGCCGTGT1250.1288992008249549No Hit
CCTCGCGGGACTGTATACAAAACTGTTCTCTTGTTCTGCCTTTTAAAATGTT1240.12786800721835526No Hit
CACTGGGATCTTCAGGTTGATATCACAATCACTTTTCTTACTTTCTGTCCAT1240.12786800721835526No Hit
ACTAATGGGACTCAGTGATAAATGAATCTCTGAAAACAAATGCTTTTACATT1230.1268368136117556No Hit
TTTATGGGGATTGGACCAAATTTGGTGTTTGTTTTCACCTTGACGCTCTTCT1230.1268368136117556No Hit
ACTTCAGGGGGGCTTTTAAACAAAGTTACCATGCCAGAAGTGTTCTTGGCAT1230.1268368136117556No Hit
TTTACCGGGATGATCTGAAAACCCAGTGGATGGCTGAAGGTGTTAAATGTGC1220.12580562000515597No Hit
TACAGCGGGAGATGCTAGATTTGAAGAAAACCTGGGTTTTATTGCATAGATT1220.12580562000515597No Hit
GCACCAGGGGAATATCAAGAGGAGGAGTTGTTGGTAATGTGTCATTGTTTGT1220.12580562000515597No Hit
TAACTAGGGCATCGAAAAACTTGAGAGTAAGGTATATTTGGGGAGAATATGG1220.12580562000515597No Hit
GCTGCTGGGATTGAGAAGTTCCATCAGAGTTGGAGATTCAGAGGAAGGAAGA1200.12374323279195669No Hit
CCACATGGGGTTTGGGAGAAAGTAAGAGAAAAGAACTAGGGTCTCTGCCTGG1200.12374323279195669No Hit
AGCAATGGGGTGGTGAAAGAGTCCAGGAGCTCCTTTAGAGATTGTCAAGTCC1200.12374323279195669No Hit
ATGTATGGGAGTGTACAATTTGGTAGTTTTTTATATATTCACAAGGTTGTAC1200.12374323279195669No Hit
GTTACTGGGATACATACTTTGATTCTTTAGTTACATAATTGATAACAAAGTG1200.12374323279195669No Hit
GATAATGGGGCACTGTTTCTTTTTGCATATAAGCATAAGTAACTTCTCTCAG1200.12374323279195669No Hit
ATGTTAGGGATGTTTTTCTAAGGAAACTTTCCACAGAATTTTAAACAACTGA1190.12271203918535706No Hit
CAGTACGGGATGAAATGAGCAAAAATTATTGAAAGTCAAAACTAACTAAAAT1190.12271203918535706No Hit
CTATTGTAGGGAGTGCTGTTTCACTGTGGTCAGAGACTGTGCTCTGCGTGGG1180.12168084557875741No Hit
AATGTTGGGCGATCTTAGACGGGGAAAGTACATAAATGCCTTTTTACAGCTT1180.12168084557875741No Hit
TACTATGGGACCTCTTTCAGGTTTTAATTCAGTTACCACCTTCTTGGTAAAA1180.12168084557875741No Hit
GCTCGTGGGAATGGCTGCCCCGAGGAAGACAACAAAGAAGCCAGTGCCCTGG1180.12168084557875741No Hit
CAAACAGGGATGGAGGTGGCGAACGCTGTGCGCGCCTCCGACCCTGCCGCCT1180.12168084557875741No Hit
TTCGCCGGGGGTGGTTGGTGTGCGGGTTTCGGTTGGAGGACTCGTTGGGGAG1180.12168084557875741No Hit
GGTGGTGGGACGGATGAGAGACTGGCTCAAGAATATCCTCATGCAGCTTTAT1170.12064965197215777No Hit
TTCAAAGGGCGGCTGCCCCGTCTGGGAAGTGAGGAGTGCCTCTGCCCGGCCG1170.12064965197215777No Hit
GAATGCGGGATTCTTGCAGATTACATGTTTTTACAGTGGCCTGCTATTGAGG1160.11961845836555814No Hit
TTACGGCGGGAGAGAAGACCACACCAAGGAGGCGGCCGCTGGCCCAGGAGAG1160.11961845836555814No Hit
ATTGTAGGGTAGTTCTTGATGGTTTTCTTCCTTATTAACAGTTGGTGTTTCT1150.11858726475895849No Hit
CTCTTAGGGCAAATGAGGTAGAGCTCACCTGTGCTCACCAGCTCCGTCAGGG1140.11755607115235885No Hit
AGACGGGGGTTGAGAATTAGAGACACAAGAGAGGCTGTGGATGGCCTATTAA1140.11755607115235885No Hit
CCGATTGGGATATTTCAAGAAAAAGAAATTATGTTAAATAGCCCTGTTTTAA1140.11755607115235885No Hit
TCATCAGGGATATGTGACTTTTTCATTTCTTTTAACAGGAAAAAATTAAAGA1140.11755607115235885No Hit
ACTGCTGGGAAGAGTGGACAGCGGGGATCTTCCAAGTCTGGAAAGTTGAAAG1140.11755607115235885No Hit
TTTGCTGGGAAATTCGATAGGGAAACAGCCTTGACCTGTACTTTACTAAATG1140.11755607115235885No Hit
ATAGGCGGGATGACTTCTACTCTTCCTTAGATATCCTCCAAATGACAAAGAG1140.11755607115235885No Hit
GCAAAAGGGTAAAAGTCAGCATTTAATATACAGTGTTTGGTTTGGAAACCAT1130.11652487754575923No Hit
AAAGCTGGGATCTGACATGGGGAGTTGTAATTGTTCATATAGGAAGGAAATC1130.11652487754575923No Hit
ATAATGGGGGACGCTCTTCCGATCTGGACCTGGGGTGCATTCAAGACAGATA1130.11652487754575923No Hit
ATTTAAGGGGCTGCCGTGTCCTGACTTCTGGAATCTTTGAGACCAAGTTCCA1130.11652487754575923No Hit
TCACAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA1130.11652487754575923No Hit
AAACACGGGGGGTTCGGTATTTTCTAACTGAAACTTGGCACTGCCATCAGTT1120.11549368393915958No Hit
GTAGAAGGGATGACAATTTTATTTAGGTATATCTCTAAGTTTTTCTTTTATT1120.11549368393915958No Hit
GGATAGGGGGAACTATGGCTGCCCAGTTAAGTAGATATCATGCTAGCCAATA1110.11446249033255994No Hit
TAGCATAGGGGTAACCTCAAGCATAGGCAACATTTTCCTATTTCATGTTATT1110.11446249033255994No Hit
TCTCCGGGTCAGGAGTGCCTCACGTAGATAGATTGAGGTTTTATAATAATCA1110.11446249033255994No Hit
TAAAATGGGACTGTGCCTGAGCTCTGGAGTGCCAAGGCCTACCCATCGCTCA1110.11446249033255994No Hit
CGCAACGGGATCACTACCTTTTATTTTGGCCACTCTGATAGGTGTGCAGGTG1110.11446249033255994No Hit
GCCCTCAGTTTCCCCTTATGATCTGGCCTCTGCCCCCTTCTCTAGCCACAGC1110.11446249033255994No Hit
AAACTAGGGCTCTTCCGATCTGGCTGTGGGATAGGATAGAGAAAAATCTGCT1110.11446249033255994No Hit
ATTCCCGGGTATTTTATGGAGTATATGTAATAAGAAGTAGCTTCTCAGTGAA1100.1134312967259603No Hit
AACACGGGGGATTTACATTTTGATAGCCATTGATGTATGCATCTCTTGGCTG1100.1134312967259603No Hit
GGGCTGGGGGAAGACTTACTGCTGAGCACATTGGCCTTTTAATACAGATGCC1100.1134312967259603No Hit
ACTATCGGGAGTGATGGAAGGCGGTTATAATGGATGGTGGTGTGTACAAGAT1100.1134312967259603No Hit
TGCCTTGGGCACAATGTGAGACAGCAACCATTTCCTTCCCAGGATATCATAG1090.11240010311936066No Hit
CGCGACGGGACATTGGAAAGTTCTGCAATAAGATTCTAGTTCTCTCTTTTCT1080.11136890951276102No Hit
AATGCAGGGACTTAAAGTAGTTCTAGTAAACATGTCCTAGAAAACAGTTGCT1080.11136890951276102No Hit
TAACTGGGGGTGCTCTAAAACTCTTCTACTTGGTGAGCATCTTGATTGGTTA1080.11136890951276102No Hit
TTGTGAGGGCATTGCCCCCACAATCCTAGGCCTACCCGCCGCAGTACTGATC1080.11136890951276102No Hit
TGCGTTAGGGAAGCTTTGATATGTGAATATACCATCACCACAATCAAAATAA1080.11136890951276102No Hit
AAGCTGGGAAGCAGATCTGGGTTGCAAACCTGCCTCTACTGCTTTACTATTC1080.11136890951276102No Hit
CAACTTGGGGTATGAAAGAAATTTTAAAAAATACAAAATATTTTCTGATTAG1070.11033771590616137No Hit
GCACAGGGGAGGAACCACCAGCTGCTGCATCCCATGGCCAGGGGTGGCGTCC1070.11033771590616137No Hit
TATCAAGGGAATCCGCCTCCAGGTAACTCGCTAATTTCCAGTGTTAAGGTGT1070.11033771590616137No Hit
GGTCACGGGTAAGGTGACTCAAGATGATACACCGAGAGAAAAATGCAAAATA1060.10930652229956175No Hit
CAGGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA1060.10930652229956175No Hit
TCTCAGGGGGCTTCCCAACATGGCGCAGTCTATTAACATCACGGAGCTGAAT1060.10930652229956175No Hit
GCTTAAGGGACTGTGGTAAGTGCTTTGCAGATGTTGTGATGTTTTCTCTTTA1060.10930652229956175No Hit
CCGACAGGGCAAGTAACTATCACAAGAACCACTGTCCCAAGATCCAAAGTCA1060.10930652229956175No Hit
CGGTATGGGAACTTAAAAGATGCCACCTCTAAGGTAGCGCTGGTATATGGTC1060.10930652229956175No Hit
AGACATGGGTAAGACCTAATTTGATCTTCATGACAACTGCATAAAATAGTTA1050.1082753286929621No Hit
TACACTGGGGAGACATAAAGTGGATATTGGCTTCAGGGGTGTTCAACATTAA1050.1082753286929621No Hit
CAGGCTCGGGATGAAAAAGTTTTTGCTGACACAGAACAAACCTGAAAGTAGT1050.1082753286929621No Hit
GCATGGGGGTCTCTCCCCCTAACCCCAGATGAATTATTGTATTCATTCCAAA1050.1082753286929621No Hit
ATACACGGGACAACTGGATGGTGGAATTTTCCAGAACTTCATTATGTGCGAT1040.10724413508636245No Hit
CTACACGGGGTATCCTATATTTTGAATTTTATACTAGTTTTTCAAAAGTAAG1040.10724413508636245No Hit
GAAATGGGATCTTTTTAGGATGGCTGAGAATTTGAATGCCCAAATCTTTCAA1040.10724413508636245No Hit
ATGCTGGGGGGAAGCGGTTGCAATCTGGATTCAGCCCGATTCCGTTACCTAA1040.10724413508636245No Hit
GTAAATGGGATTTTTTGAAGATTGTTGGGTACTTTATTGTCTCTTGAGATTC1040.10724413508636245No Hit
GCGTTGGGGCAAGTCATGGACCACATAATCTTTTCCCCCACTGGCAAAGTGA1030.10621294147976283No Hit
ATACTTGGGAGAGCTAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAG1030.10621294147976283No Hit
TCGAAAGGGACAGTAATAGATTATGAAGGATGAATTATTTGAAAGCTATTTT1030.10621294147976283No Hit
TACTCAGGGATTTTGAACAGTTAAACATCTTGAGTATAAAAAGAACCCCAAT1030.10621294147976283No Hit
ATTCCTGGGCACTTTGGGAAGTTATTTCTTTATTTTAATGATGTATCATTGT1030.10621294147976283No Hit
TAATCAGGGGACTTTAGCATGTAAAATCTTACATTTTTCTTGCCTTGGGAAA1030.10621294147976283No Hit
GAGTTCCGGGATTTATGCAAGTCATGCTTTCATTCTACATGTAGTTAAAACT1020.10518174787316319No Hit
TACGTGGGGTAATGAACTTACCAATATAAAAACATGATTTGGTTCCTGAGCT1020.10518174787316319No Hit
CATGTTGGGGGACGAACGGGAGCAGATGGCCATCAGTGGCGGCTTCATCCGC1010.10415055426656354No Hit
TTCTAGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1010.10415055426656354No Hit
TGGTCAGGGATTTGTGTCCAACTGAAATATAATTGTCATTAAAATAATTTTA1010.10415055426656354No Hit
AACTAGGGAGAAGCTGAGCAAAGAATTCTTTCAGCCCTGAAAAGGAATCTGG1010.10415055426656354No Hit
TCTCCAGGGGTCCTTGAGAAGCATTAAGAGGTAGGGAGTGAAACAGGTGAGG1010.10415055426656354No Hit
GCGCTAGGGACGGTTAAAACAAAGAAAATGTTGTCAACGTTGACGCCAACAT1010.10415055426656354No Hit
TAAATGGGTGAATTCAATGATGTGAAGACCTTGCTCAGAGAATGGATAACTA1010.10415055426656354No Hit
AGCCGTGGGTCCTCCCTCCTACCCTGGCACGTGGAATAGGGCTTACTCACCC1000.10311936065996391No Hit
TCAGTTGGGATTCTTCAGGTTATTATGTTACTAACATTCCTGTTTTTATCCA1000.10311936065996391No Hit
GATTTCGGGATTTTTAGAGATTAATTTGTGTAGATTGTGTAAATTCCTGTTG1000.10311936065996391No Hit
TTAAAAGGGGGATATTGTGAGTTAATACTTTAGAAAAAAATCCATCAACTCA1000.10311936065996391No Hit
GACTTAGGGGGTTTATTCTCAGGTAATAATTTGGGAAGTGTAAGAAAATATG1000.10311936065996391No Hit
AGCACTGGGACATGGTAAAAAGTTGTGGCAAAGATGGCTTCCATATCCGGGT1000.10311936065996391No Hit
AGCGCTGGGCAACTTTATTATCTTACACTTCTGGAGTGCGGATTCCTAAAAC1000.10311936065996391No Hit
TATGAGGGTTGTTTGATGAGACTGACATTGCCTAATTTCAAGCTTTTTGAAT1000.10311936065996391No Hit
AAGCAACGGATGGCAGAATATTTATACATGAAAAAATAATTTTGCAAATATT1000.10311936065996391No Hit
GTGAACAGGGTAATGGATAAAGGTATACCTCAGACTTCACTGTGCTCACAAA990.10208816705336426No Hit
GTAAATGGGATTTGAATCTTTGTACCTCCATACAAGTGTTAGCCTGCCAGGC990.10208816705336426No Hit
TCGTTTGGGATTCTCCTGAGGCATTAGATAGTTTTTCTATTATAAGTGACAG990.10208816705336426No Hit
ATGCGCGGGGCTTGGGACAGACCATAAATGATGGAAAATGTCAACACAAAAA990.10208816705336426No Hit
CTTTCCGGGTGTGATGTGAAAAATCAATTTATTATCCTTGGTGCTTTCCCCC990.10208816705336426No Hit
GCTTCGGGGCACAGTGACAAAGGAAAAGTGGTGCCGTTATGCAATCCATTTA990.10208816705336426No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAAACTCGTATGC990.10208816705336426No Hit
GCTGCCGGGGGGAACATATGTGAGAGGAGAGGTTCAAATTTTTGGCTGGTGT980.10105697344676462No Hit
TATGCAGGGAGGAAATGAATATATGTGAGTTCTAGCATTTCTCCCCACCCCC980.10105697344676462No Hit
ACATAAGGGGCTATGTTTCAGTGGAAGAAATTCTTTGAAGTAGATGTGAGTG980.10105697344676462No Hit
CTGCGCAGGGACCTGTACACACTTCCTCGTTTTGTAATGAGATTTACTTACA980.10105697344676462No Hit
GGGACTGGGCTACTGTGGTTCCATATTGAGCAAACATTCAAGTCTCATGAAA980.10105697344676462No Hit
ATAGCAGGGATTTAGAGAATGCAGATACAAGTTGAGTTGTAAAGCAAACATT980.10105697344676462No Hit
TGACAAGGGGTGAGTTTGTTTTCAGAAAAATTCATTGATTAGAAAGTGATAA970.100025779840165No Hit
GGGTATGGGGACCCACTGGAATTTCTATAAGGCTGGTACCCTTCCCAGAGTT970.100025779840165No Hit
TTTGCTGGGAAGAAATTCTGTACCATCTGAAAGTAGTTCTTGTTGGTGCCTT970.100025779840165No Hit
ATCTTAGGGCCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCAC970.100025779840165No Hit
TCAGCACGGGGGAGGTAGATTGGGTGGTTTCAACAGGAACGCATCCACTGTC970.100025779840165No Hit
GCTATGGGGATACAAAGGTAGCCCCATTATCATAGAACTTGATAATCATGGG970.100025779840165No Hit
CGGGTGACCCGCCCACCTCAGCCTCCCAAAGTGCTGGGATTACAGGTGTGAG970.100025779840165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)