FastQCFastQC Report
Sat 31 Dec 2022
SRR5848943.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848943.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90506
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC3790.4187567675071266No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3500.3867146929485338No Hit
AGTTGAGGGCAATATGATAGAATTGAAATGCTAAAGAACTATAAGGAAGGCC1800.19888184208781737No Hit
AAGCAGGGGAATCTTAAGTTCTGGGATACATGTGCAGAACATGCAGGTTTGT1200.1325878947252116No Hit
TCCAAAGGGGAGGATTTGCATTCTAGGTGTATAATTTTTGGAGGAATGTTTA1140.125958499988951No Hit
TCCAATGGGTAAACTGAGTATTATTAAAATGCTGTTACCTAGAAGAACCTCT1040.11490950876185005No Hit
CTACGTGGGGTAATACTACATTACAATTTTAAAACCATTTTAAAATAAAAAT920.10165071928932888No Hit
GGTATCCGGGATGTGTACTAAACACATTCACATTTGCCAATGTTGCCTCTAT910.1005458201666188No Hit
GGCAATGGGAGAGATACCATAGTTGACTAATCCTTGATTTCTGAAATACAAT910.1005458201666188No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)