FastQCFastQC Report
Sat 31 Dec 2022
SRR5848942.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848942.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135433
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC3910.28870363943795085No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3440.25400013290704626No Hit
TCTGCTGGGCTAGACGGGGGATGTGAGAGGAATTTGTAGGCATGATGTCACT1590.11740122422157082No Hit
GCACGTGGGGGCAGAGCTGCAGCCGCCGAGCCGGACGTGTCCGCGAAGATGG1570.11592447926280891No Hit
ACAATTGGGTGCTTTCCTTTTATTACTAAATTTTCATCTGTTTTCTTGGAAC1570.11592447926280891No Hit
CGCTATGGGCTGCAGCCCCAATTATAAATACCATATGGACCATACTTTTATA1560.11518610678342797No Hit
AGACATGGGATGACTTCCTGCTGGGCTGGTTTTAAGTGAAATATGGTGGGGA1540.11370936182466607No Hit
GCGCTAGGGACGACATTGCATTTTCAAGCCACTGGGTGTAAAAATAAAATTA1500.11075587190714228No Hit
GCGTGCAGGGAAAGTAGTAGACTACACAACAGCGAAGGAATTTGCTGATTCC1500.11075587190714228No Hit
TACTAGGGGTATCCAATCAAGACAAGGAAACAACAGAAAGAGAAAACTACAG1460.10780238198961847No Hit
TATTATGGGATCTGCATTTGGGCTTGGCCCCATCCATGGACTCTTGCCTCGG1450.10706400951023753No Hit
GGCTGAGGGGTTTCTGTTTATTCCAGAACTATATTATCGAACTATATTATCA1440.10632563703085658No Hit
TTAGTTGGGCCTCCCGGGATGACCTCAGGACTCTGTCGGTTTCCCCTCTTTA1360.100418657195809No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)