FastQCFastQC Report
Sat 31 Dec 2022
SRR5848941.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848941.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135422
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC4090.3020188743335647No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3400.25106703489831783No Hit
GCACGTGGGGGCAGAGCTGCAGCCGCCGAGCCGGACGTGTCCGCGAAGATGG1840.13587157182732496No Hit
CTCTGCCGGGGGGAAAATTTTTGCGAAGTAGCCTTCTGAAAAAAGTCTAGTA1530.11298016570424303No Hit
AGACATGGGATGACTTCCTGCTGGGCTGGTTTTAAGTGAAATATGGTGGGGA1530.11298016570424303No Hit
TTGCTCCAGGGACACTTGAAATGAAACACAGTTTTAAAACTTGCTTTGTTTA1480.1092880034263266No Hit
TGCAAAGGGAGTTTTAAAATATTTTTCTCTGCTGCTGATTCACTGACAGTAT1460.10781113851516001No Hit
GGCCAGAGGGCATCCCCCACAGTCTCAGCATGTACCGCCAAGATGAGCTCAG1450.10707270605957674No Hit
TCTCATGGGATTCGCGCTCTGCTCAGTGGGTAAAATTAGATGCATGTTGACC1450.10707270605957674No Hit
ATAGACGGGTTGTTAATTAACCCTTATGGAAAGTTATTTCTGTTTTAGAAAC1450.10707270605957674No Hit
AAGAGGGGAAGGTTACAAAGCTAAGAAGTGGCCATGCCCAGACCTGGACTTG1410.10411897623724359No Hit
CTAGGGGCAAGACCAATTGATCCTACCCTCCTGTGTTTTCTTCTAAGAGCTT1410.10411897623724359No Hit
TATTATGGGATCTGCATTTGGGCTTGGCCCCATCCATGGACTCTTGCCTCGG1390.102642111326077No Hit
ATATCTGGGACTTATAGAAACCTTGTCAACTTGTTTGGGTCAAAAGTTAGAT1390.102642111326077No Hit
TTTTAAGGGACTCTCGTTACTTCTCGCTGTTGGAAGCATCTCTGGGTTTCTT1380.10190367887049372No Hit
TATTGCGGGCAGCAGTGCTCAGCACCATGAGGCCCAACACACACAGATCAGA1380.10190367887049372No Hit
ATGGTGGGGGATATTTATTTTTTAAGAGCTCTGCCACAGCTGGTTACCATTA1370.10116524641491044No Hit
TTACAGGGGATTGGTGAAATGGGAAAAGACTACATTTATGCCGTAACACCGT1360.10042681395932714No Hit
ACAGCAGGGCCTAACATCATCGCATTCAAGTAGGTCAAGGCTTTTTTTTTTT1360.10042681395932714No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)