FastQCFastQC Report
Sat 31 Dec 2022
SRR5848940.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848940.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133591
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC4020.3009184750469717No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3620.2709763382263775No Hit
ACAGCAGGGCCTAACATCATCGCATTCAAGTAGGTCAAGGCTTTTTTTTTTT1790.13399106227215907No Hit
TGCAAAGGGAGTTTTAAAATATTTTTCTCTGCTGCTGATTCACTGACAGTAT1660.12425986780546594No Hit
GCACGTGGGGGCAGAGCTGCAGCCGCCGAGCCGGACGTGTCCGCGAAGATGG1640.12276276096443624No Hit
GGCCAGAGGGCATCCCCCACAGTCTCAGCATGTACCGCCAAGATGAGCTCAG1480.11078590623619855No Hit
TAACTCGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1440.10779169255413913No Hit
CTCTGCCGGGGGGAAAATTTTTGCGAAGTAGCCTTCTGAAAAAAGTCTAGTA1430.10704313913362426No Hit
ACTCGGGTCATCAAGGTCTGATTACAAAAATTCAAAAAATTACAACCTCAGG1360.10180326519002028No Hit
GCGACCAGGGATCAGAACAATCAAAAACCCAGTAACATAAATTTTTGCTCTA1360.10180326519002028No Hit
GGTGCTAGGAACTTACTCTGATGAATTTGGGAACCTAGATTTTGAGCGATCC1340.10030615834899057No Hit
GACACTGGGTAAGGAATGAATGCCATTAGATGGGCTACTGAGAGTACAGGGA1340.10030615834899057No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)