FastQCFastQC Report
Sat 31 Dec 2022
SRR5848939.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848939.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137690
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5690.4132471493935652No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5250.3812913065582105No Hit
AAGACAGGGCATAATGGGTGGTTATTTAAGATGAGACTTTGAACTAAAAAGT1830.13290725542886192No Hit
GCTCTAGGGAATATAAATGGACCTATGTGGAAACAAGCTACCCTGAGACAGT1600.11620306485583558No Hit
GCATCTGGGCACTTAGACTTGATTAGAATTTAATGTAATTTTTAAAATTCCT1590.1154767957004866No Hit
GATCATGGGGCTATTGAATTTCTCAACTTTGGTCATTTCACTGCTTTTTAAA1590.1154767957004866No Hit
GCTGCTGGGAGAGCTGAAAAACTCACTACAAGAATTTCATAATAAAATTGGA1560.11329798823443969No Hit
TGTTGCGGCATTGATCTAACCTTTTTCCCCCCCATTTCAGTTGCAGTTTTTG1550.11257171907909072No Hit
ACTAATGGGGAGACTGGTCCACCACAATGTGACACGGTGGGAAACTTTTGAT1530.11111918076839276No Hit
TTGCTTGGGGACCGATTTTAGAGTCTGCCTTTTCACTGCTTCAACCTAAAAC1530.11111918076839276No Hit
ATTATTGGGGAAGAATCTAGAAATTTAACAACATAAACCAGAAACAACGACT1510.10966664245769484No Hit
ACACCCGGGTCTCAACGTGGGGGTAGCCGAACGCTGTGGCGGGGCAGGCGAG1510.10966664245769484No Hit
ACTCTTGGGATTTCCTCACCTTTCGTAGACTGTCTTAGCTTCCCATTTTATA1500.10894037330234584No Hit
TGCATAGGGACTGTCACAATGAAAATCTTGACAATTTACTTAACAAGTAACC1480.10748783499164791No Hit
ACCTATGGGGCAGGTGGCCAACTCAGCTTTTGTGGAGCGAGTGCGGAAACGG1470.10676156583629894No Hit
TTCTGAGGGACATACCTGTGCTGCTGCTTAAAGGAATAGGGTACAAAGTTTA1470.10676156583629894No Hit
CTCTGGAGGGGAAGGATTGTAATCCAAGCCTGGGAAAATCTTAAATTTCTTT1450.10530902752560098No Hit
CGATTTGGGACAGGAACGCCCCACCACACGTGGCTAATATTTGCGTTTTTAG1440.10458275837025202No Hit
ACACTAGGGAGGGACTCAAGTAATATCACAGTACCCTTCTCTCCCGTGTTAC1430.10385648921490305No Hit
AGACAAGGGTCGTGCGTGCCTCCCGCCTGCCCATGTCCATCATCATCGTAGG1420.10313022005955406No Hit
TCTATGGGGTACAGTAAAATAAACATGCTATATTGTTAGCAGTCTTTTCCAG1420.10313022005955406No Hit
ACAGCAGGGTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTG1410.1024039509042051No Hit
AGTAGGGGGGTGAGTTTGGATTTCCTAGGAATGTATGCCTGAGGGAGAGATT1410.1024039509042051No Hit
AAGCATGGGAGGTAGACATGAACTTTTCCTGCACAAAGCCTTGCTTTTAGAG1390.10095141259350716No Hit
TGTTTAGGGGTCTTTCCAAAGCGCTGAGGCTGTGCACCTATTCTGTAGTTGC1390.10095141259350716No Hit
GCTTATGGGCAGCAAAAAAGTGGGGGGCATATTGTAGTCCTGTCATTTAAGT1390.10095141259350716No Hit
TATCTTGGGCTCCTGCCTCAGTCTCCCAAGTACCTGGGGATACAGGCGCCCG1390.10095141259350716No Hit
ATCTGCGGGGAGACTGAAGAGGAAGAAAAGAACCTCGAAGAGCAAACAGAAA1380.10022514343815817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)