FastQCFastQC Report
Sat 31 Dec 2022
SRR5848938.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848938.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences137832
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5670.4113703639212955No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT4900.35550525277149No Hit
GCTCTAGGGAATATAAATGGACCTATGTGGAAACAAGCTACCCTGAGACAGT1740.1262406407800801No Hit
ACAGCAGGGTACGTGCTGAGCTCGCGGGTGCGCACGGGCCGCAGCATCCGTG1730.12551511985605665No Hit
AAGACAGGGCATAATGGGTGGTTATTTAAGATGAGACTTTGAACTAAAAAGT1640.1189854315398456No Hit
ACACTAGGGAGGGACTCAAGTAATATCACAGTACCCTTCTCTCCCGTGTTAC1570.11390678507168146No Hit
GCATCTGGGCACTTAGACTTGATTAGAATTTAATGTAATTTTTAAAATTCCT1570.11390678507168146No Hit
TCTATGGGGTACAGTAAAATAAACATGCTATATTGTTAGCAGTCTTTTCCAG1560.11318126414765803No Hit
TAAATGGGATTATTTAAATTGTTCATCACAGTAGAAGTCATTATTTTTAAAT1530.11100470137558767No Hit
ACTCTTGGGATTTCCTCACCTTTCGTAGACTGTCTTAGCTTCCCATTTTATA1530.11100470137558767No Hit
GGACTCGGGCGATCTATGTTTGGGAGGTTATGATTTATAGTTATTTTGATAG1490.10810261767949389No Hit
TGTTGCGGCATTGATCTAACCTTTTTCCCCCCCATTTCAGTTGCAGTTTTTG1470.10665157583144699No Hit
TTAAAGGGGGATCTGAATTAGGGTGATTTGTGTCATAGACATAATTGATCAA1450.10520053398340007No Hit
CTCTGGAGGGGAAGGATTGTAATCCAAGCCTGGGAAAATCTTAAATTTCTTT1440.10447501305937663No Hit
TGCATAGGGACTGTCACAATGAAAATCTTGACAATTTACTTAACAAGTAACC1420.10302397121132974No Hit
CTTGGAGGGCAAAAGAAGCAGCAGCAGCAGTAATACAAGGCAAATAGAAATC1420.10302397121132974No Hit
TTTACTGGGATCTAAAGACGGGAGATGATCCCTAGTGATGACCTTTCCCAAA1410.10229845028730629No Hit
ACTAATGGGGAGACTGGTCCACCACAATGTGACACGGTGGGAAACTTTTGAT1400.10157292936328283No Hit
GCAAAAGGGCAATGAGGAAAATCCCCACAGATTTGACCTCCTGGTCCGTACC1400.10157292936328283No Hit
GCTGCTGGGAGAGCTGAAAAACTCACTACAAGAATTTCATAATAAAATTGGA1400.10157292936328283No Hit
CAATGTGGGGCAGATCAGGCAGGACATTCTGGAGGAATCATGCCCTTGGAAG1390.10084740843925939No Hit
GCTCTGGGGAGCGAGGCTGCGGCAGGAGGAGCGGGAGCAGGAGCAGCGGCAG1380.10012188751523593No Hit
CACGCAGGGGGGGCGCTGCTTGCTAAAGAGCCGAGCACGCGGGTCTGTCATC1380.10012188751523593No Hit
TAAGTACGGGATAGTCGTCTTGTTATTAACAAATTCATGTTAACAGTAAAAA1380.10012188751523593No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)