FastQCFastQC Report
Sat 31 Dec 2022
SRR5848937.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848937.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135660
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5210.3840483561845791No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT5020.37004275394368275No Hit
TACATGGGAACTGCCTGTTTTCCTAAATCAAACCCATCCTCAAAGGATGAAG1680.12383900928792571No Hit
GCAAAAGGGCAATGAGGAAAATCCCCACAGATTTGACCTCCTGGTCCGTACC1590.11720477664750112No Hit
GTGCCAGGGGGCTGAGATGGGGACTGAGGGCAGCTTGGTGTAGGCCTGCAGG1560.11499336576735958No Hit
GCTGCTGGGAGAGCTGAAAAACTCACTACAAGAATTTCATAATAAAATTGGA1530.11278195488721805No Hit
ACTAATGGGGAGACTGGTCCACCACAATGTGACACGGTGGGAAACTTTTGAT1520.11204481792717086No Hit
CACGCAGGGGGGGCGCTGCTTGCTAAAGAGCCGAGCACGCGGGTCTGTCATC1520.11204481792717086No Hit
TGTTGCGGCATTGATCTAACCTTTTTCCCCCCCATTTCAGTTGCAGTTTTTG1470.10835913312693499No Hit
GCTCTAGGGAATATAAATGGACCTATGTGGAAACAAGCTACCCTGAGACAGT1450.10688485920684063No Hit
GCATCTGGGCACTTAGACTTGATTAGAATTTAATGTAATTTTTAAAATTCCT1420.1046734483266991No Hit
TCAAACGGGAAGCATCAAGCACGCAGCAATGCAGCTCAAAACGCTTAGCCTA1410.10393631136665193No Hit
TTAGTGGGGATTGGATTTGGATATTTGAGGTAAAATTTCATTTTGTTATATA1390.10246203744655757No Hit
ACTCTTGGGATTTCCTCACCTTTCGTAGACTGTCTTAGCTTCCCATTTTATA1390.10246203744655757No Hit
CAATGTGGGGCAGATCAGGCAGGACATTCTGGAGGAATCATGCCCTTGGAAG1370.10098776352646321No Hit
AAGACAGGGCATAATGGGTGGTTATTTAAGATGAGACTTTGAACTAAAAAGT1370.10098776352646321No Hit
GCTAATGGGTCCCGCTGGAAGCCTCCATCTCTTTGGAAAAACAGTTAGTCTG1360.10025062656641603No Hit
TCTATGGGGTACAGTAAAATAAACATGCTATATTGTTAGCAGTCTTTTCCAG1360.10025062656641603No Hit
AACACAGGGAGGGAACAAATGTGATCTGACCACAAAGAAAGTAGTAGACTAC1360.10025062656641603No Hit
TTTACTGGGATCTAAAGACGGGAGATGATCCCTAGTGATGACCTTTCCCAAA1360.10025062656641603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)