FastQCFastQC Report
Sat 31 Dec 2022
SRR5848930.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848930.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120020
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6200.5165805699050158No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT6170.5140809865022496No Hit
ACTTGAGGGTGGAGAGAGGGAACTGGAATCTAACTTGGGGATGGGGGTGGAT2090.17413764372604565No Hit
TATAGCGGGGAAATTTTGCTTTATTAGATGAGTTTAAGTAAATTTTATATGC2030.16913847692051326No Hit
TAGTCAGGGCTTTAAGAATTTGTATACTTAATCTCAAAATTCAGAGCTTTTG1980.16497250458256957No Hit
AGATTAGGGGGGTTGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCA1980.16497250458256957No Hit
AACACTGGGCTGTCGCAGTTCAGCAGAATCTAAACACCCACATTCTGTCCTT1970.16413931011498084No Hit
ATGTGTGGGAGATAAAGGATGTATCAGTCAGTTCTTAACTCTTGTATATTTT1960.16330611564739209No Hit
GCCTATGGGATTGATGACATATCGCTCTGATGTTTGTAATTACGCGCAGTGT1900.1583069488418597No Hit
TAGAAAGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1850.154140976503916No Hit
AATTGTGGGCAACAGTCATATCTTCTGATGTGGAGATTTGAGAAGCATTTGT1800.14997500416597234No Hit
GATGTTGGGATTTCTGGTCTATGTTCTTCTATCATAGTTTACTGTTTTCCAA1770.14747542076320613No Hit
TAAACAGGGAGGCTCCGGCAAGTCTTCCCTGTTGTTGGTGCTCTTCCGGCTG1770.14747542076320613No Hit
ACACAAGCAGCTGATTGCCCCAGTGGATATCTAAGAAAGGCTTCATTAAGGA1760.1466422262956174No Hit
TAAAATGGGAGAGGAGATGATGGAATATTTGATGACAATTTTATTGAGGAAA1750.14580903182802868No Hit
GTATCCGGGATGTCAGGGCAGTGAGTGAAGCCAAAGTACATCAGCACCCACT1750.14580903182802868No Hit
TCATTAGGGAACGAGTGAAATACATGTATTTTGCTATATTTATTCTTTCTGG1740.14497583736043992No Hit
TTCATCGGGATTGGATATCCAGAAATCGTTCATCTTGTATCACTGAAACTTT1740.14497583736043992No Hit
TAGTGTGGGGATATAGTTGTCTCTTCTGTGGCTTCTTGGTAGCGATGAAATG1730.1441426428928512No Hit
TGTACCTGGGCAGTGTGATAGAAGAATTGGAGGGGGTCCTCACTGAAAGTAA1730.1441426428928512No Hit
GTTAGAGGGAATTGTAGTTTGGGAGAATCCTCAAAAATAGGGATATCAGATA1710.14247625395767372No Hit
TGAACCAGGCATGTGAGTAAGCCCAGTCAAGATCATGAGAGCCACAGTGAGG1710.14247625395767372No Hit
TTGTTAGGGGCCTAGAAAAGAAGCAGTAATCTACCTCTGCCGATAACCTGTT1700.141643059490085No Hit
GGCATTGGGCTGGCAGAGGGCAGGAAATGCACGTCAAATGAGTGAATAAATA1700.141643059490085No Hit
TCTGGGGGAAAATATTAGGGTCAAAACATAGGCTTATGTTATGTTTTGTGAA1690.14080986502249623No Hit
CAACAGCGGGCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1690.14080986502249623No Hit
GTTGACGGGAAGGATATTTATAAAAACCCACAGCAAATGTGGTAATGATGAT1690.14080986502249623No Hit
AGTCACGGGACTTACTTTATATGCATTCATTGTAAATGCTGATATCGTCTTA1690.14080986502249623No Hit
TAAACGGGGGGGTTCTCAAATCCAGATTTCATTCTGCAGTCCCGGCAGTGTT1680.1399766705549075No Hit
GCTCTAGGGGTTTGATATAATTGAAAGAGTTGAGTGGATAAACAGTCTTCCA1660.13831028161973005No Hit
CACTGCGGGATGAGCAGTCTGGTGGAGCCAGAGAATGTCCCCTCCCATCAGC1660.13831028161973005No Hit
CACTGCCCTAAAGGAAAGAACACAGGCCTGTCTGGCTTTGCCACCTGCTAAT1660.13831028161973005No Hit
ACATAAGGGGCAGGACCTGCACATGTTACAGGTTTGCCAAAAGCATCTTTTT1650.13747708715214133No Hit
TACTAAGGGTTTCTTTATTAACTTTTATTTTAAGTTCAGGGATACAAGTGCC1640.13664389268455257No Hit
GGTGCTGGGGATTCTGTCTCAAAAAAAATTAAAATAAATAAGCAAACAAAAT1640.13664389268455257No Hit
GGTGTTGGGGTACTCAATCAGGAAGCTGGAGGTGTTAGACACCAGCCCCCTG1640.13664389268455257No Hit
TCTGGTGGGAGGCAGGAAGTGTCGGCGCCGCCACTGTCCGGCCACAGCCTAA1610.13414430928178636No Hit
TTCTGCGGGATGAGGAAACGCCTTTTAACAGAAATCTTACCTTTTTAAGCTT1600.13331111481419763No Hit
CTTATCGGGACCTTTACATTTTGCTATTTTTTCCTCAGTGTTTTCTTTTGTA1600.13331111481419763No Hit
GCTTCGGCCTATCAAAGTGCTGGGATTACAGTTGTGAGCCACCACACCCAGT1580.13164472587902015No Hit
TTATATGGGAGGCCTCTTCAGACCAACATTTGGTAGCTGCTAGTAGAGACTT1570.13081153141143143No Hit
GCCTTTGGGGACATATGTCTGTATCTTTTTATATGGAAGTGGTAGAGATATA1560.1299783369438427No Hit
CAGCTGGGGCAACCTGGTTCCACTTCCAGATCTGCCTGTCCTCAGCTCACCT1560.1299783369438427No Hit
TAACACGGGTTAGTGCTAGATACTAGAAATTTTCACTTTTCCACTGATCAGA1550.12914514247625394No Hit
TGCTTCGGGGTATATGAAGAACTGGCTTTGGATTTAACTGAAAACTTAGGAA1540.12831194800866522No Hit
CCTTGCGGGCACCTCTGCCCCGACAGAATGGGGCTGAATCAGCTCCAGGAGG1530.1274787535410765No Hit
TGCGAAGGGGTTTTTAAAAAGCTTTTCATCCTTACAGTGCAAATTTTAAGAT1520.12664555907348776No Hit
GCATTTGGGACTCTGCCTGAGATCTACTTCTGTGCGCACTGGTTTAGAATGT1520.12664555907348776No Hit
CACACAGGGATGAAGCTTGGAAAATATATCTTGAAATTTTTGTTATCAGATC1520.12664555907348776No Hit
ACAAAAGGGTTGGAGAAAATGTGTATTGTCTTGTGCCATTCTGCAATATGAA1520.12664555907348776No Hit
GCTCTGGGAGATATTTCTATTTCTTAATATCTGCTAGGGTGAGCTTCCTGCT1520.12664555907348776No Hit
GCATTGGGTTGCTAAGGAAAATGATATCTTAGAGATTCCTAGAATCATTTAG1510.12581236460589904No Hit
TCTCGTGGGCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTG1510.12581236460589904No Hit
GTATCTGGGATCACTCTTTGATTTTCCCCTGTGTTTGTTTCCTAGTTTTGCC1500.12497917013831028No Hit
TTCATTGGGCAAAGAAAGGAACTAGATTTGTTGTGTAGATGGCCTTGGTGTC1500.12497917013831028No Hit
TACCACCGGGACATGGGACTCTGACATGTGCCCATAGCTAGGGTGGCAGTAG1490.12414597567072154No Hit
ACTGACGGGGATGGACCTACATGTCATTACATTGAAATATGCCAGGCCCAGA1480.12331278120313281No Hit
ATTTGTGGGATTGTTCTGTCAGAAAACCCTCTCCCAGTTCCCCTGCAGCTCT1480.12331278120313281No Hit
CGAGCTGGGCTGCTGTTTTTGTCTACATTATACTGAAATTAAATGTTTATAA1480.12331278120313281No Hit
CTCGGTGTGGGACTTAATGGTATGAATATGATGAGTGACAGTTGAAAGAAAT1460.12164639226795534No Hit
ACGTTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1460.12164639226795534No Hit
TATGATGGGAGGCGTATGCGCCAGCAGCATTCTATGCCTGTGACTCCTGAGT1450.1208131978003666No Hit
ATGCATGGGTAAAGTTTAATTTACATAGTTACAACTAGTTGAATTGATTTCC1440.11998000333277786No Hit
GAAAGTGGGGATAGTTTAACAATAACACTTAAAACTTACATCATTTGATTGT1440.11998000333277786No Hit
GAGTGAGGGGAAGCAAAAATCTTTTACAGTTATGGTGATCATTATTGAAAAT1440.11998000333277786No Hit
GGTAGCGGGAGATACCTGCATTTGAAGCATTTATATTAGAAGGAAGCCTTTT1430.11914680886518914No Hit
CAGATAGGGTCAGTACAAAAATAACTTTTTGTGTTTTAAATTTGTGATTTTG1430.11914680886518914No Hit
TGTCCGGGGTCATTTAATGTTCACAACAATCCTGCCATGTAGGAAGAATTAT1420.11831361439760041No Hit
ACATACGGGGGACCTAAGATTTGCATTAGTTTTTCTCCTGCACCCTTAAAAG1410.11748041993001165No Hit
TTAGTTGGGCCTTATCTTTGATTGTGTGTTGAACATTATACGAAATTATAGT1400.11664722546242293No Hit
TTCTGAGGGGGGCACAATAAACAATTATAGCATTTTCTCTATCTTTTAGACT1400.11664722546242293No Hit
CTTTGCGGGACATCAGCGGGTCCAGGCCCAACCGACAGACTATGGGGGCTCC1390.1158140309948342No Hit
GCTTGGGGTGATTTCAATCTTAAATTTGTTAAGACTTGTTTTATGACCTAAT1390.1158140309948342No Hit
CCTAACGGGATATTAGAATGCATAAGACATGTTTTTCCTTCATATGCTAGAC1380.11498083652724546No Hit
AAATGAGGGTATGATGGAATGCTGAACTAGAACCAAAACAGATAAAGCTAAA1380.11498083652724546No Hit
CGCTTGGGGGCACTTGGGGTTTATTAAAAAGCAGTGAAGCCTGGGAGAGTTC1380.11498083652724546No Hit
AGTAGAGGGTGTCTTTATAATAGAATGATTTATATTCCTTTAGGTGTATACC1380.11498083652724546No Hit
TCTCTAGGGACTTCGGAGGCTGAGGTGGGGGAATTACTTGAGCTCAGCAGTT1370.11414764205965673No Hit
CAACAAGGGTGACTTAACTCAATCCTTTCTTTCCCAGGGAATTTGTTCTTTG1370.11414764205965673No Hit
GTTATGGGGCCAGTTCCACATGGCTGGGGAGGCCTCACAATCACAGTGAAAG1370.11414764205965673No Hit
TAAACAGGGAATGCCTGGATGCCTGGGTAGCCAGTTTGGGGAGTGGGCTGCA1370.11414764205965673No Hit
ATTAGAGGGCATCTATATGGCTCGCAACCCCAAGGATCTGGTGGTGTCTTAT1370.11414764205965673No Hit
GCATTTGGGAGAGGAAAAGAAGCCACAGAGAGGTTAGTGAAGTAACTTGTCC1360.11331444759206798No Hit
CGAAGAGGGGGCTGCTCAACATTAATTGTCTCCTTTTTGTGAATTTATTTGT1360.11331444759206798No Hit
ACAAACGGGCGATCTTTGGTTGGGGATTGATTTTGTATGTTAGACTTTTTAC1360.11331444759206798No Hit
AGCTGTGGGCATGAGATTGCTAAATTATTATCCCCAACTTACAGAAAAGAAA1360.11331444759206798No Hit
GTGTGAGGGATTATTATTTTCCTAAAATTATTGAACTTTATTCCTTGTCATA1360.11331444759206798No Hit
GCGTGGGTCCTCACCAAATGAGCTCAGCTCATTATTACACAGATGACAAATG1340.11164805865689052No Hit
AACACAGGGATCAATTGTATTAATTAGATAATGGTTAAATTTTTATTTTTTT1340.11164805865689052No Hit
AACACCGGGGTCCCTCTGCTTCCTTACTTTTTTTCCTTTTTGAATTCTATCT1330.1108148641893018No Hit
TACGGTGGGATCTTAAATTCTTTGAGCTTTAATCTTGCCATCTCTAAATGAG1320.10998166972171304No Hit
GACTTCGGGGAATGAATGAATGATTTGCTTTATATTAAAGTCATAAGTTCCT1320.10998166972171304No Hit
AGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.10998166972171304No Hit
AATACGGGGAGCCCTCAAACAAATCACCTCTTTTATATCACTGTCATCAAGA1320.10998166972171304No Hit
ATCGCTGGGAAGGGATATTATGAACAACTTTACGGCCACGAATTTGGTAACC1320.10998166972171304No Hit
AGCATGGGGGGTACTTTGTGAAATGAATTCTTGAGATACTTAGGCACTACAC1310.1091484752541243No Hit
TATTTGGGGGAGGAAAAAGAGAAAAATGAAATACTGAATCAGAAATGAAAAA1310.1091484752541243No Hit
TGCGGGGCACCAATGCAGAGGTAACTCTCAGGCAGCTAAGCAGCACCTCAAG1310.1091484752541243No Hit
GGAAGAGGCTGTTCTGGAAGGAAGGGCAGGTGAGTGTGGATGGAGCCCAAGC1300.10831528078653557No Hit
CGTGCCGGGGATCAGACGAATTAGTAAATGTACAGTGCTTAGAATAATGCCC1300.10831528078653557No Hit
TTTAACGGGGATATTTAATTACTGTGTATTTAGTGTTTTGATTTTCTATAAT1300.10831528078653557No Hit
CAGGCAGGGCAAACCATGCCTGTATTAATATATTTTAGGTATTACCTTTAGA1300.10831528078653557No Hit
AACAATGGGGGAGGGAGGGAGAGAGGGAAGGAGGGGGAGAGAGAGAGAGGGA1300.10831528078653557No Hit
TTGGTAGGGATGGCACAGCTCCAGGAGATTATGAGAACATGTGCCCAAGTGG1290.10748208631894685No Hit
TGCTAAGGGTAGGGTCCCGCAAGGGGACACAAACTCATCCACATGTGCTGAA1290.10748208631894685No Hit
TCGCGTGGGCAGCTTAAGTTTGGGGAGGGTAGAAACGCCGTTTCCTTGGGAA1290.10748208631894685No Hit
CATTTAGGGGATGGTAACTAAGGAAACCTAAATACCATGAAATACATTGCTA1290.10748208631894685No Hit
TATAATGAGGGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAAT1290.10748208631894685No Hit
GCTTTAGGGAACAGACTTTATTTGGATTTGACGGTGTGTCCTCTTCCTTCTT1290.10748208631894685No Hit
GCTTTGGGGGACACAGGTAAGGTTTTCATCTGGGTGTTGACTGTGAATAACA1280.10664889185135812No Hit
TAGCTGGGGGATCCCGAATTTTGATGTACCTTAAACTTCCACATCACTGCAC1280.10664889185135812No Hit
TTGCTTGGGTAGCACTAATCCTTTCTCCATATAAAGCAATCCAATGACTCTT1280.10664889185135812No Hit
GTTGCCGGGAGGCGTCCGCCACCATGCCCAGCCAATTTTTGTACTTTTAGTA1270.10581569738376936No Hit
TAAGCTGGGATAGAAAATCATACCTAAGCTACATTTTCTTTTCAGTCAGAAC1270.10581569738376936No Hit
ATTAATGGGCGCTCTTCCGATCTACGACAGGGAATATGTTTGATATTTATTG1270.10581569738376936No Hit
TATACTGGGAGATGCTGCTGTCTTCACTAACAAAGACTGTATCCTGTGTTTT1270.10581569738376936No Hit
TTATCAGGGCACCTCTTTTCCCTTGGTGTTTATGTTTCATACACAAAAACCA1260.10498250291618064No Hit
ATATGAGGGGTATAAAAGTGGTCAGAGTATATGAACGCATTTTACAGCTATT1260.10498250291618064No Hit
AGAACAGGGGATTTTTAGAAAGTTAAACAAATAACATATGTTTGCCAGGCTA1260.10498250291618064No Hit
AAGTGTGGGAGCTACAAAAACCTACTTTGGTATTTATTACAGAAAAGTGCTC1260.10498250291618064No Hit
ATTAAGGGGAAGTCAACTGAATGCGCAAAGATGATATAATTTTGGATGTAGA1260.10498250291618064No Hit
CATTAAGGGAAGCTATGTTCAGATAGAAGCCTCGAAATTCCTGTGAATTGTT1260.10498250291618064No Hit
TTACCAGGGCCTCCAGACTTCAACTTAATCTCTCCTTTCTGATCTTTACCAG1260.10498250291618064No Hit
GAACTAGGGGAGACAACCTTTATTAAAAATGCCTACTTTCATAAGTGAAAAT1260.10498250291618064No Hit
TGTTCAGGGAAATTTGAAAGAGATTGTAGAGTCTTGAATTCTGACATGCATG1260.10498250291618064No Hit
TTATCCGGGACCATAGGAATGCATTTGAAATCCTTTATGTGCTTCTTTGGAA1260.10498250291618064No Hit
GACGCAGGCCCAGAGGATATAGTAGGGATTAGCATGATCCCACTAGCTACCT1250.1041493084485919No Hit
TGACTAGGGCGATCTTGTACGGGACAGATATTTACAGGTTTTTGGGAATAGG1250.1041493084485919No Hit
GTATTAGGGATATTTGCAAAGGACTAGTATCTAGAATACATTTAAAAGTCTT1250.1041493084485919No Hit
CTTGAAGGGGACACAGTAAAATTTCTTTCTTAAATCAAGATACTGGTGATTC1250.1041493084485919No Hit
CGAGCAGGGGATACTCTGCTACCATTTTGGCAGTGTTATAAGCCTATTGATT1250.1041493084485919No Hit
ATTAAGGGGTATCAGGAATGTGTTTAAAACAAGAAGAGAACTTTTCTAAGGA1250.1041493084485919No Hit
GGTGCTGGGCTGTTAGAGTGGCTTCAAAACATGAGCCTTATGGGAAGTAGCC1240.10331611398100317No Hit
CTCGAAGGGCATCTAGCTGTGGTCAAGATGGACTTCAAATTCACGTATACCA1240.10331611398100317No Hit
AAGCACGGGGATTAGAGGATGATTTGATAGATTTGGGTAAAGCTTTTGTTTA1240.10331611398100317No Hit
GAGCCTGGGCGACAGAGGTAGACTCTGTCTCAAAATAAGAAGAAAAAAATAC1240.10331611398100317No Hit
ATAACTGGGTGATTCTTTTGAGTGTTTCCAGTACATGGTCTTTGTATATATG1230.10248291951341444No Hit
CAGCTAGGGCAGTTCTTCTATGAATGAATCTGCTCCCACTGGTATTACTGAT1230.10248291951341444No Hit
AACAGTGGGATAATTTAATAATAGATATTGATTTACTTTGTAAATTATGAAG1230.10248291951341444No Hit
TATTGTGGGTATAGTTGTTTATCCATTACCACATTTTTGTATTTTAATAGTC1230.10248291951341444No Hit
GGATTCGGGGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTATACA1220.10164972504582569No Hit
CTTGCCGGGAGATAAATACCTTTCAAGTGTGAGCTTAGACGTCAACCCTAAA1210.10081653057823696No Hit
CGCTATGGGGGAGCCAAAATATTGTCCAGCCCCCAAGGACTTCTGTTAACTA1210.10081653057823696No Hit
AAACACGGGTTGGTGGGGTATAGTATCTAGCTGTTACTCTGAGTATATGTGA1210.10081653057823696No Hit
AAACCAGGGATTAGTGCTAATTGAACCATTGATAGTAAAACACTCTTAGAAA1210.10081653057823696No Hit
CGTTTTGGGTCTTGGAAAAAAAGTGAAACTTAAGTCAAACCTCTGCCAATGT1210.10081653057823696No Hit
CTTTTTAGGGAGTTAATCATTTAACTACTGCTATTGTGAGGATTAGGTGGGA1210.10081653057823696No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)