FastQCFastQC Report
Sat 31 Dec 2022
SRR5848928.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848928.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119197
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT6010.5042073206540434No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC5640.4731662709631954No Hit
GATGTTGGGATTTCTGGTCTATGTTCTTCTATCATAGTTTACTGTTTTCCAA2060.1728231415220182No Hit
ATGTGTGGGAGATAAAGGATGTATCAGTCAGTTCTTAACTCTTGTATATTTT1970.16527261592154163No Hit
AACACTGGGCTGTCGCAGTTCAGCAGAATCTAAACACCCACATTCTGTCCTT1930.1619168267657743No Hit
TCTCGTGGGCCTTACAATGGATCTTTTGGAATCTTGTTTTCCTTATGTCTTG1890.15856103761000695No Hit
TGTACCTGGGCAGTGTGATAGAAGAATTGGAGGGGGTCCTCACTGAAAGTAA1870.1568831430321233No Hit
TAGTCAGGGCTTTAAGAATTTGTATACTTAATCTCAAAATTCAGAGCTTTTG1860.15604419574318146No Hit
GCCTATGGGATTGATGACATATCGCTCTGATGTTTGTAATTACGCGCAGTGT1860.15604419574318146No Hit
GTATCCGGGATGTCAGGGCAGTGAGTGAAGCCAAAGTACATCAGCACCCACT1850.1552052484542396No Hit
AATTGTGGGCAACAGTCATATCTTCTGATGTGGAGATTTGAGAAGCATTTGT1850.1552052484542396No Hit
GCTTCGGCCTATCAAAGTGCTGGGATTACAGTTGTGAGCCACCACACCCAGT1830.15352735387635597No Hit
CACACAGGGATGAAGCTTGGAAAATATATCTTGAAATTTTTGTTATCAGATC1830.15352735387635597No Hit
ACTTGAGGGTGGAGAGAGGGAACTGGAATCTAACTTGGGGATGGGGGTGGAT1800.15101051200953045No Hit
TAAACGGGGGGGTTCTCAAATCCAGATTTCATTCTGCAGTCCCGGCAGTGTT1790.15017156472058862No Hit
ATGCATGGGTAAAGTTTAATTTACATAGTTACAACTAGTTGAATTGATTTCC1770.14849367014270493No Hit
CAACAGCGGGCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATTTAC1740.14597682827587943No Hit
TTCTGCGGGATGAGGAAACGCCTTTTAACAGAAATCTTACCTTTTTAAGCTT1710.14345998640905394No Hit
CTTATCGGGACCTTTACATTTTGCTATTTTTTCCTCAGTGTTTTCTTTTGTA1700.1426210391201121No Hit
TAAAATGGGAGAGGAGATGATGGAATATTTGATGACAATTTTATTGAGGAAA1680.14094314454222842No Hit
ATCGCTGGGAAGGGATATTATGAACAACTTTACGGCCACGAATTTGGTAACC1680.14094314454222842No Hit
GGTAGCGGGAGATACCTGCATTTGAAGCATTTATATTAGAAGGAAGCCTTTT1670.14010419725328657No Hit
GAGTGAGGGGAAGCAAAAATCTTTTACAGTTATGGTGATCATTATTGAAAAT1660.13926524996434472No Hit
GTTAGAGGGAATTGTAGTTTGGGAGAATCCTCAAAAATAGGGATATCAGATA1650.1384263026754029No Hit
ACACAAGCAGCTGATTGCCCCAGTGGATATCTAAGAAAGGCTTCATTAAGGA1650.1384263026754029No Hit
CTTTGCGGGACATCAGCGGGTCCAGGCCCAACCGACAGACTATGGGGGCTCC1640.13758735538646108No Hit
TTGTTAGGGGCCTAGAAAAGAAGCAGTAATCTACCTCTGCCGATAACCTGTT1630.13674840809751923No Hit
CCTAACGGGATATTAGAATGCATAAGACATGTTTTTCCTTCATATGCTAGAC1630.13674840809751923No Hit
GGCATTGGGCTGGCAGAGGGCAGGAAATGCACGTCAAATGAGTGAATAAATA1630.13674840809751923No Hit
TTCATCGGGATTGGATATCCAGAAATCGTTCATCTTGTATCACTGAAACTTT1610.13507051351963556No Hit
AGTCACGGGACTTACTTTATATGCATTCATTGTAAATGCTGATATCGTCTTA1610.13507051351963556No Hit
TATAGCGGGGAAATTTTGCTTTATTAGATGAGTTTAAGTAAATTTTATATGC1590.1333926189417519No Hit
TAGAAAGGGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1580.13255367165281004No Hit
GCTCTAGGGGTTTGATATAATTGAAAGAGTTGAGTGGATAAACAGTCTTCCA1580.13255367165281004No Hit
TGAACCAGGCATGTGAGTAAGCCCAGTCAAGATCATGAGAGCCACAGTGAGG1580.13255367165281004No Hit
TCTGGGGGAAAATATTAGGGTCAAAACATAGGCTTATGTTATGTTTTGTGAA1560.1308757770749264No Hit
ACGTTCGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1560.1308757770749264No Hit
AGTAGAGGGTGTCTTTATAATAGAATGATTTATATTCCTTTAGGTGTATACC1560.1308757770749264No Hit
GAAAGTGGGGATAGTTTAACAATAACACTTAAAACTTACATCATTTGATTGT1550.13003682978598455No Hit
ATTTGTGGGATTGTTCTGTCAGAAAACCCTCTCCCAGTTCCCCTGCAGCTCT1540.1291978824970427No Hit
TACTAAGGGTTTCTTTATTAACTTTTATTTTAAGTTCAGGGATACAAGTGCC1530.12835893520810088No Hit
AATGCTGGGGAACTTCCGGCCAGCGCAGCCATTTTGGCTTCCTGACCTTGGG1530.12835893520810088No Hit
GTTGACGGGAAGGATATTTATAAAAACCCACAGCAAATGTGGTAATGATGAT1530.12835893520810088No Hit
ACATAAGGGGCAGGACCTGCACATGTTACAGGTTTGCCAAAAGCATCTTTTT1510.1266810406302172No Hit
AGAGGCGGGAACCATATATTGAAAAAGTGCTAATAATTTTTTAATGTAGAGG1500.12584209334127536No Hit
GTTGCAGGGAACACTGTAATAATTTAAGCCTCCTGTTTTATAATCTTCTATC1500.12584209334127536No Hit
AGCTGTGGGCATGAGATTGCTAAATTATTATCCCCAACTTACAGAAAAGAAA1490.12500314605233354No Hit
GGTGTTGGGGTACTCAATCAGGAAGCTGGAGGTGTTAGACACCAGCCCCCTG1490.12500314605233354No Hit
AGATTAGGGGGGTTGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCA1480.1241641987633917No Hit
TAGTGTGGGGATATAGTTGTCTCTTCTGTGGCTTCTTGGTAGCGATGAAATG1480.1241641987633917No Hit
TGGCATGGGCGATCTCTTGTCGGGGGAGCTCCTCTGGCAGGTTCTAAAAGCA1480.1241641987633917No Hit
CCTTGCGGGCACCTCTGCCCCGACAGAATGGGGCTGAATCAGCTCCAGGAGG1470.12332525147444985No Hit
TAAGTCGGGCTTCTTAAGAAATAAAATAAAATGAAAATAAAATAAAATTCTT1470.12332525147444985No Hit
TTTAACGGGGATATTTAATTACTGTGTATTTAGTGTTTTGATTTTCTATAAT1470.12332525147444985No Hit
TGCTTCGGGGTATATGAAGAACTGGCTTTGGATTTAACTGAAAACTTAGGAA1470.12332525147444985No Hit
GCATTTGGGACTCTGCCTGAGATCTACTTCTGTGCGCACTGGTTTAGAATGT1460.12248630418550803No Hit
ATTAATGGGCGCTCTTCCGATCTACGACAGGGAATATGTTTGATATTTATTG1460.12248630418550803No Hit
CTCGGTGTGGGACTTAATGGTATGAATATGATGAGTGACAGTTGAAAGAAAT1460.12248630418550803No Hit
AAATGAGGGTATGATGGAATGCTGAACTAGAACCAAAACAGATAAAGCTAAA1460.12248630418550803No Hit
CTTTTTAGGGAGTTAATCATTTAACTACTGCTATTGTGAGGATTAGGTGGGA1460.12248630418550803No Hit
ACGCTTGGGGTATCTTGATATCTCGAACTTTCAAAATATATTTTCAGTACAC1460.12248630418550803No Hit
ACGTGGGGAAAATAAAGGGCAGTGCCCTGAAGTGTTGATTAAGTAAAGGCCA1450.1216473568965662No Hit
TGCGAAGGGGTTTTTAAAAAGCTTTTCATCCTTACAGTGCAAATTTTAAGAT1440.12080840960762435No Hit
CAACAAGGGTGACTTAACTCAATCCTTTCTTTCCCAGGGAATTTGTTCTTTG1440.12080840960762435No Hit
GCATTTGGGAGAGGAAAAGAAGCCACAGAGAGGTTAGTGAAGTAACTTGTCC1430.11996946231868251No Hit
CATTTAGGGGATGGTAACTAAGGAAACCTAAATACCATGAAATACATTGCTA1430.11996946231868251No Hit
GCTCAGGGTAGGATTTCATTTTTGTACACTCCTGATTAATAATCATATTCTG1430.11996946231868251No Hit
TGTCCGGGGTCATTTAATGTTCACAACAATCCTGCCATGTAGGAAGAATTAT1420.11913051502974069No Hit
ACAAACGGGCGATCTTTGGTTGGGGATTGATTTTGTATGTTAGACTTTTTAC1420.11913051502974069No Hit
TGACTAGGGCGATCTTGTACGGGACAGATATTTACAGGTTTTTGGGAATAGG1410.11829156774079885No Hit
TAGCTGGGGGATCCCGAATTTTGATGTACCTTAAACTTCCACATCACTGCAC1410.11829156774079885No Hit
GCCTTTGGGGACATATGTCTGTATCTTTTTATATGGAAGTGGTAGAGATATA1400.117452620451857No Hit
GTATCTGGGATCACTCTTTGATTTTCCCCTGTGTTTGTTTCCTAGTTTTGCC1400.117452620451857No Hit
GGAAGAGGCTGTTCTGGAAGGAAGGGCAGGTGAGTGTGGATGGAGCCCAAGC1400.117452620451857No Hit
CGTGCCGGGGATCAGACGAATTAGTAAATGTACAGTGCTTAGAATAATGCCC1400.117452620451857No Hit
TAAACAGGGAGGCTCCGGCAAGTCTTCCCTGTTGTTGGTGCTCTTCCGGCTG1400.117452620451857No Hit
TATGATGGGAGGCGTATGCGCCAGCAGCATTCTATGCCTGTGACTCCTGAGT1390.11661367316291517No Hit
TTATATGGGAGGCCTCTTCAGACCAACATTTGGTAGCTGCTAGTAGAGACTT1390.11661367316291517No Hit
ACAAAAGGGTTGGAGAAAATGTGTATTGTCTTGTGCCATTCTGCAATATGAA1380.11577472587397333No Hit
TGCGGGGCACCAATGCAGAGGTAACTCTCAGGCAGCTAAGCAGCACCTCAAG1370.11493577858503151No Hit
GCCTTAGGGATTTCTCGGACTCAAAATCCCTTATGGCAGAAAAACATTGCTC1360.11409683129608966No Hit
CAGCTGGGGCAACCTGGTTCCACTTCCAGATCTGCCTGTCCTCAGCTCACCT1360.11409683129608966No Hit
TTACCAGGGCCTCCAGACTTCAACTTAATCTCTCCTTTCTGATCTTTACCAG1360.11409683129608966No Hit
AGCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1360.11409683129608966No Hit
CTTGAAGGGGACACAGTAAAATTTCTTTCTTAAATCAAGATACTGGTGATTC1360.11409683129608966No Hit
GCTTTAGGGAACAGACTTTATTTGGATTTGACGGTGTGTCCTCTTCCTTCTT1360.11409683129608966No Hit
ATTAGAGGGCATCTATATGGCTCGCAACCCCAAGGATCTGGTGGTGTCTTAT1360.11409683129608966No Hit
GTCAATGGGATTCATTAGTTTGTCATAAAGTTTTAGGAAAGGTAGGACCCGG1350.11325788400714783No Hit
GGATTCGGGGGACACTCAAAGTGTGGAATGCAGAGACTGGAGAATGTATACA1340.11241893671820599No Hit
GCATTGGGTTGCTAAGGAAAATGATATCTTAGAGATTCCTAGAATCATTTAG1340.11241893671820599No Hit
GGTGCTTGGGGAGAATAAATGCAGTAATCCTCTCCCAAATAAGCACACACAT1340.11241893671820599No Hit
AAACTGGGGGCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTG1340.11241893671820599No Hit
CATTAAGGGAAGCTATGTTCAGATAGAAGCCTCGAAATTCCTGTGAATTGTT1330.11157998942926417No Hit
TTGCTTGGGTAGCACTAATCCTTTCTCCATATAAAGCAATCCAATGACTCTT1330.11157998942926417No Hit
TAGTTCGGGCTAAATATAAATTAAATAATTTGTAAATGTTGTTACAGATTAA1320.11074104214032232No Hit
ACATACGGGGGACCTAAGATTTGCATTAGTTTTTCTCCTGCACCCTTAAAAG1320.11074104214032232No Hit
GTGTGAGGGATTATTATTTTCCTAAAATTATTGAACTTTATTCCTTGTCATA1320.11074104214032232No Hit
TAACACGGGTTAGTGCTAGATACTAGAAATTTTCACTTTTCCACTGATCAGA1320.11074104214032232No Hit
TTAAAGGGGAGAGGCTCAACTTCCTGTACCAGGCCGCCCATTGTGTCCTTGC1310.10990209485138049No Hit
TCATTAGGGAACGAGTGAAATACATGTATTTTGCTATATTTATTCTTTCTGG1310.10990209485138049No Hit
GATACGGGGTCCCTGGCATTGGTCCACATGCAGGTGAAACAGCTTCTTGTGT1310.10990209485138049No Hit
CGCTTGGGGGCACTTGGGGTTTATTAAAAAGCAGTGAAGCCTGGGAGAGTTC1310.10990209485138049No Hit
TATTGTGGGTATAGTTGTTTATCCATTACCACATTTTTGTATTTTAATAGTC1310.10990209485138049No Hit
ACATTAGGGGCAGTGTTGGTAATAGTAGATCATTTCTTAAGCCATGATACTT1300.10906314756243865No Hit
GCGTGGGTCCTCACCAAATGAGCTCAGCTCATTATTACACAGATGACAAATG1300.10906314756243865No Hit
TCGCGTGGGCAGCTTAAGTTTGGGGAGGGTAGAAACGCCGTTTCCTTGGGAA1300.10906314756243865No Hit
TTTGCTGGGCTGGAAGATTCTTACTGTTAACCAAATTTTGAGCAAGGAGTCT1300.10906314756243865No Hit
TTACTTGGGCAAAACATATGTAAAGAAAGAATCTCAAAGTGGAAATGTTGTG1300.10906314756243865No Hit
AATAACGGGGTAATCATGGCCAGTCAATAATTATATAAAGGAGTTCAAATAC1300.10906314756243865No Hit
AGCATGGGGGGTACTTTGTGAAATGAATTCTTGAGATACTTAGGCACTACAC1300.10906314756243865No Hit
TTCTACGGGAACCAACCACATTTCAAGTGGTCAGTAGCCACATGGGTGAGTG1300.10906314756243865No Hit
TACCACCGGGACATGGGACTCTGACATGTGCCCATAGCTAGGGTGGCAGTAG1290.10822420027349683No Hit
TTGGTAGGGATGGCACAGCTCCAGGAGATTATGAGAACATGTGCCCAAGTGG1290.10822420027349683No Hit
GTTGGCGGGGTACTGTCATCCTTTAAAGTTTTGTGGGTTTTCTTAGTTTTGA1290.10822420027349683No Hit
GTGCCCGGGAGCAGGGGAAAGGAGATTATTTTAGGTATCTTTCTGAAGTGGC1290.10822420027349683No Hit
TCTCTAGGGACTTCGGAGGCTGAGGTGGGGGAATTACTTGAGCTCAGCAGTT1280.10738525298455498No Hit
TAATGAGGGGTCTACAATGTTAGGAAGATGGTTGTGATATTTTCATGGAACT1280.10738525298455498No Hit
ATTTATGGGGGGATGAGGAAGACCCCCTCGGGCTCTCGGCTGACCCCCACCT1280.10738525298455498No Hit
GGTGCTGGGGATTCTGTCTCAAAAAAAATTAAAATAAATAAGCAAACAAAAT1280.10738525298455498No Hit
AGTGCTGGGAATGAATTTATGAATTGGAAGATAGACCCAAGGAATTCCTCCA1280.10738525298455498No Hit
CTTAATGGGACTATACCCTAAGGGGTTGAAGATTAGGAAGAGTGAGGCAAAA1280.10738525298455498No Hit
TACGCCAGGGCTGACATTGGTCACATATTTGTCATTTCCTTTAAATTTAAAT1270.10654630569561314No Hit
TGATTTGGGATTGTACACTGAAATGCTTAAATGTATCTGAAAGCACAAGGTG1270.10654630569561314No Hit
TTCATTGGGCAAAGAAAGGAACTAGATTTGTTGTGTAGATGGCCTTGGTGTC1270.10654630569561314No Hit
CGAAGAGGGGGCTGCTCAACATTAATTGTCTCCTTTTTGTGAATTTATTTGT1270.10654630569561314No Hit
GTACAGGGGGAGTTTTCTTATGCTGAGCTTTTGAAACTTTTCATAATCTGAT1270.10654630569561314No Hit
TATACTGGGAGATGCTGCTGTCTTCACTAACAAAGACTGTATCCTGTGTTTT1270.10654630569561314No Hit
TATTTGGGGGAGGAAAAAGAGAAAAATGAAATACTGAATCAGAAATGAAAAA1260.10570735840667131No Hit
CTCGCAGGGCTACCTGTCCCAGTACACGAAAGGGCGCTGGTTGGCATTCTTC1250.10486841111772949No Hit
TGCTAAGGGTAGGGTCCCGCAAGGGGACACAAACTCATCCACATGTGCTGAA1250.10486841111772949No Hit
GCTTTGGGGGACACAGGTAAGGTTTTCATCTGGGTGTTGACTGTGAATAACA1250.10486841111772949No Hit
AACAGTGGGATAATTTAATAATAGATATTGATTTACTTTGTAAATTATGAAG1250.10486841111772949No Hit
GTCTTTGGGATTTCTTGCTGCATTTCATCCTTAAAACAGTGCTGTGAAGCAG1250.10486841111772949No Hit
GCAGCAGGGCCTACGTGTTATGTGCTAACAAAGAGAATGCCTACTAAATAAA1240.10402946382878764No Hit
GCTTGGGGTGATTTCAATCTTAAATTTGTTAAGACTTGTTTTATGACCTAAT1240.10402946382878764No Hit
CTTTTAGGGATGGTGGCCTAATGAAAGCAGGATGCTTATGTTTATTGTTATT1240.10402946382878764No Hit
AAGCACGGGGATTAGAGGATGATTTGATAGATTTGGGTAAAGCTTTTGTTTA1240.10402946382878764No Hit
GAGCCTGGGCGACAGAGGTAGACTCTGTCTCAAAATAAGAAGAAAAAAATAC1240.10402946382878764No Hit
CGAGCTGGGCTGCTGTTTTTGTCTACATTATACTGAAATTAAATGTTTATAA1230.1031905165398458No Hit
GGGATTATAAGCATGAGCCACCACACCTGGCCAATTTTTGAATGTGAAGAGA1220.10235156925090397No Hit
CTCGAAGGGCATCTAGCTGTGGTCAAGATGGACTTCAAATTCACGTATACCA1220.10235156925090397No Hit
TGACTTGGGCGGCCTGGCAGCCGGCTTTGCCATCGGCATCGTGGGGGACGCT1220.10235156925090397No Hit
TTATCCGGGACCATAGGAATGCATTTGAAATCCTTTATGTGCTTCTTTGGAA1220.10235156925090397No Hit
AACACCGGGGTCCCTCTGCTTCCTTACTTTTTTTCCTTTTTGAATTCTATCT1210.10151262196196212No Hit
GGACACGGGAGGTTGTAGGGAGATATGTATGTGTGCTCCCTGGGGTGGGGAG1210.10151262196196212No Hit
AAACACGGGTTGGTGGGGTATAGTATCTAGCTGTTACTCTGAGTATATGTGA1210.10151262196196212No Hit
AACTATGGGCATCATCTCTTATCTCTATACAGGGACTAGGTAATAAAACCTT1210.10151262196196212No Hit
GTACCTGGGACGTGAAGCCAGCAATTGTTTCGCAATTCGGCATTTTGAAAAC1210.10151262196196212No Hit
TGCATGGGGGAAGCAGGTCATGCCAGACCAAAGAGTGATCTACTACTATGCA1210.10151262196196212No Hit
CGAGCAGGGGATACTCTGCTACCATTTTGGCAGTGTTATAAGCCTATTGATT1210.10151262196196212No Hit
TAGGCTGGTTCTGTTGCTCGTATCAATAGGTTATCTGTGTCCCTCATCTTGG1210.10151262196196212No Hit
AAAAAAGGGATTTTTGGTTTTATTTTTTGTTGTTTTTAATTAATTTTTTTAG1200.10067367467302028No Hit
AGGCGTGGGTCGCGCGGGGTATTCTGATAAAATGACTATATTGGTGGTGAGA1200.10067367467302028No Hit
TGATATGGGTTACTAAAAAGAGCTGCCATCATGGCCCAGGGAGGCGGGTGAA1200.10067367467302028No Hit
CACGACGGGCCTGGCTTTTCTAGCCAATCTGGACTCTTTAAGTGTCAGGAGA1200.10067367467302028No Hit
ATTGTTGGGTAGCCACTTTGATGTCTGCCTTGGGAGTAAAACATATAATAAG1200.10067367467302028No Hit
TCTGGTGGGAGGCAGGAAGTGTCGGCGCCGCCACTGTCCGGCCACAGCCTAA1200.10067367467302028No Hit
TATAATGAGGGAAAGGAACTGTAAGCTTTTATCTTTTAACCAACTGAACAAT1200.10067367467302028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)