FastQCFastQC Report
Sat 31 Dec 2022
SRR5848921.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848921.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49860
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC4300.8624147613317288No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3660.7340553549939831No Hit
TTTCTAGGGAACGGGCCTAGGGAATAGAAGCATGGTGAAAGGGGAATGGAGA1050.210589651022864No Hit
GAAATAGGGATTTATTTACTTTTTCATACTTTTTTCCTTTCTATTACATTGG1000.20056157240272765No Hit
TCTCTGGGGATGGTGGGCTTTGCGGGAGAGAAGGGCACAGACTGGCTCCCCT930.1865222623345367No Hit
CGATATGGGGGGAAGGCCAATCAATGGAGCAGTCAGAACACACACATCTATC910.18251103088648216No Hit
ACTTTTGGGAGTACAATTAGAATACTTTAATCTCTTTGAGATGCTGAAAATA880.17649418371440032No Hit
TTGACAGGGGATCTTATTCAGGGATAGTGTGGGTGTCTATGTAATTACTAAA870.17448856799037304No Hit
CTTTTAGGGCGTTACCATGACTCTGACACTTCTAATCATTTGGTCTTGAGAA830.16646610509426393No Hit
CGACTAGGGAGCATTTCAATTCTTAGGTATATACACAACCAATAAACATACA800.1604492579221821No Hit
CCATAAGGGGAGGCGCTGAGGCTGCTGGAGGCGCGGGCCGGGCGGTGCGCAC800.1604492579221821No Hit
ACCTAGGGGGATGGGGAACAGAAAGGGAGTATGGAAAGAAAAATTATAAGAG780.15643802647412755No Hit
GTAAACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC760.152426795026073No Hit
ACACGTGGGGAAGAAAAGACCGGGATCAGCAAAACATGAAATCGTTATAGCA760.152426795026073No Hit
GTGTTGGGGAAGGCGGACGACTAGAGTCGTTGGGCCCGGCGCGACCCGCAGG740.14841556357801847No Hit
AGTCACGGGGGCTGACGACTATGAACAGGTCAAGAACGTGGCCGATTACTAC740.14841556357801847No Hit
AACGACGGGGACTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATC720.1444043321299639No Hit
TGTGGCGGGATGAGCAGTGGGAGTGCAGCGCCAGGTGACAAGATGTTGTGAG710.14239871640593663No Hit
TCGTTGGGGCACAGTGGGGAGAAACCCTATGAATGTAATGAATGTGGGAATG710.14239871640593663No Hit
TAAACAAGGGAGGTGAAAAATTCACAGAACAAAAATGTCGATTATTTTAAAA690.13838748495788206No Hit
GCGTGAGGGAACCAGGGAGGTAAAAGATCTTTACAGTGAGAACTGCAAAACA690.13838748495788206No Hit
GATTTTGGGAAGTTGAGGACTGTCTACACAATGGATTGAAGGGAGGGCAAGA690.13838748495788206No Hit
ATCTATAGGATTTTTTGTTGCGGAAAGCTATCTTGTGCATTGTAGATGTTTA690.13838748495788206No Hit
TGAGACGGGATCAGCTTAAAAAAACCCTTCTATGAAAACTTTGAGAGAAAAT680.13638186923385479No Hit
ACTGTAGGGCAAACTGAGAAACGTAATGGGCTAGTAAGATGGATGGAAAAGA680.13638186923385479No Hit
TAGGTGGGGACCAGTCACTTTGTAGCTTTGCAGTGGTTCAGCAATTAGCCAT670.13437625350982751No Hit
TGGCTTGGGAGAGTTATTGATGATGGTTAAATGAGATAATCGGAGAAGAGTG660.13237063778580024No Hit
GTTGTCGGGCATTTAATTAAGATTTAATCATTTGCTAATGGAAATCTTACCA660.13237063778580024No Hit
TGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCAGCCAACACTAGTCATTT660.13237063778580024No Hit
CACGATTGGGTTCTCCAGAAAATGAAACTGCTGATAACAGTTTTTCTCAGGA660.13237063778580024No Hit
TAGCATGGGAGAAGGACTTGCTGAATACATTGTTGAGTTTCTGAAGAAGAAG660.13237063778580024No Hit
TTTGTTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT660.13237063778580024No Hit
TCTGTGGGGCAGACTACCACAGAGCCCACACAGCCAGGAAGTCTGTCTCCCC660.13237063778580024No Hit
ACATACGGGGTATTTTACCTCTCAGTTTCGGTGTGGTCACTTACTATCTTTA650.13036502206177294No Hit
ACATGTGGGGGAGCCTGGCCCAGGAGAACCAGAGATGATCCCGAGGCAGCTG650.13036502206177294No Hit
TTATATGGGGTCTGATTAATATTCATCTTTTATTGAAGAAAGTGCCTTCTGA650.13036502206177294No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAA650.13036502206177294No Hit
GAAATGGGGGTACCTGTAAATTACATCATTTTCAGGATATCCCTTGGAATAC650.13036502206177294No Hit
TTTTATGGGGGAATAACATAACTTTTACTGTCCTATACTTAAAAAAATCTGT640.12835940633774567No Hit
CAATGTGGGACAGAAATTTGAGGAGCCAGATATTGTAGAAGATGGTATGTGT630.1263537906137184No Hit
AGACAGGGGCTGGTTGGAGAGGAGAGAGAAGAGGCTCAGAGACAAGTAAGAG620.12434817488969113No Hit
CTCACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA620.12434817488969113No Hit
TTGTCTGGGGAAACACTACCAAGAGAATGAAGAGACAAGCCAAAGAACGGGA610.12234255916566386No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAAACTCGTATGC610.12234255916566386No Hit
AGTATAGGGCATCTCCCTGACCTTACAGAGAGAATCTGATGGCTCTGCATCC610.12234255916566386No Hit
GAAACAGGGGATCTTACCAAGGGACACTACATTGTACAAGGAATGAAAGTGT600.12033694344163659No Hit
CTTATCGGGGATGAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATT600.12033694344163659No Hit
ATGCCAGGGCACACTGGGTGTTAGCTTTCAACATAATCGGGGGGCGAGGAGA600.12033694344163659No Hit
TACGTTGGGTGTGGGGGATGTTCTCCGTTATTACCAGTTACATCCTCTTCAG600.12033694344163659No Hit
TGCGCGCGGGGAGCATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTC590.11833132771760932No Hit
TATGCACTAGGGCATGAGCCACAGCCACCACACTCTGTCCTCAAGTTCCTGC590.11833132771760932No Hit
GTTTCGGGAGGCATTTAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG590.11833132771760932No Hit
TAAGGTGGGGCCGAGGGCAGTGGTTGGCTTTTGCGTTGCAGATTGCAGGGTA590.11833132771760932No Hit
AAAGCCGGGCTTCAGGTTTTGTTGTCTTGGCTAATTCAAATAATTCAGGTTA590.11833132771760932No Hit
TGATAGGGGGCGCAGCCGCGGTGGGAGGAGGCACCGGCCGCGCGGCGGGAGG580.11632571199358203No Hit
TATGGCGGGTAGCACGGGCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGG580.11632571199358203No Hit
GCCAAGGGGCTGTAGAGTATTCCATGGTATATATGTACCATATTTTCTTTAT580.11632571199358203No Hit
CAAATAGGGAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCCACT580.11632571199358203No Hit
TTGAGAGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC580.11632571199358203No Hit
TTGTAGGGGGTCTGCTACGGGGTTATAAAATTTAAGCAATTGTTAGTGTTTA570.11432009626955475No Hit
GAGCATGGGGTGTGCCTGTGTTCTTACTACCATCACAGCTGAATGCAATGAA570.11432009626955475No Hit
ATTTTGGGGCAGCAAAATAGAACAACTTATAACTGGATGACCCTCAAAAACC570.11432009626955475No Hit
ACGCTGGGGATTATTTTCTGTGAAATAAAAATGCAGCACTGCATTTTTTTAA570.11432009626955475No Hit
TGATCCGGGGATCTGATGACATGGGCTTTTTAAAAGATTACTATTTTTTTTA570.11432009626955475No Hit
GGCCCACAGTAGCCCCTCAAGTGGAGATTTGAATGACCAGGTCCACCTAAAC570.11432009626955475No Hit
TAAGATGGGGCCTTCCGGCGGGTGACATTCAGCCGGCGGTTCGGGGCGACGG570.11432009626955475No Hit
AGCGCAGGGGGAGGCTTTGCAGGAAACTCTCATTCGTGCACTAAGGACCTTA560.11231448054552746No Hit
CTAACTGGGGCAGTTCTGTGACTTCCAAGCCAGGAATTAAATGCTCTTTTTA560.11231448054552746No Hit
ACTACAGGGGGTGTAGTTCAAGTCTTTCTTTATATAGAAACCTCCTTACTTA560.11231448054552746No Hit
TGAGTTGGGACCATAAAGGAAACATTTTATCTCTTCTGTGTCAGTCTGTGAC560.11231448054552746No Hit
CTAGGTGGGAAGCAATACACACCTACCTGGAAAATCGTGTTGAGTCTGTGGG550.1103088648215002No Hit
TTAGTTGGGCAACTAATTTCTTTTTTAGTATTTAATTTTGAAATAATTTCAG550.1103088648215002No Hit
GACACAGGGGTATATCATGATATGCCTATCATATCATATGCATATGATAACC550.1103088648215002No Hit
ACTAATGGGGACTCACTCGCGCGTTAGGAGGCTCGGGTCGTTGTGGTGCGCT550.1103088648215002No Hit
GCGGCCGGGTGTAGGGACCTGCTTAGTTCCTGAAAAGGGGAGTGTAACTAGT550.1103088648215002No Hit
AGCTGAGGGACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTA550.1103088648215002No Hit
TAACTTGGGATATTTCTGTTACTGTGGCCCAAGGACAGCTAACTGGCTATGT550.1103088648215002No Hit
ATTGCGGGGAAATATGAGACGACATACATAAACTAAGAAACAAAAATATTGC540.10830324909747292No Hit
TTGGCTGGGATTACAGGCATGAACCACCGCACTGGCCTTTGATGATTTCAAT540.10830324909747292No Hit
TTTCTGGGAGCTTGATGAGGATAGCATTGAATCTGTACATTACTTTGGGCAG540.10830324909747292No Hit
TGCTACGGGAGGATGAATGATAAAACAAGTACAAGAATATCAATCATATTTT540.10830324909747292No Hit
ATTGACGGGCTATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTC540.10830324909747292No Hit
AACGTAGGGGATCTGGCCGCGGGATCCTGCAACTTTACTAAATTTGTTTATT540.10830324909747292No Hit
TATCACGGGTCCTCTTTGTTTATCTCTGAGACAGTCCAACCTTGAGAATAGC540.10830324909747292No Hit
GTTGGTGGGGACCAAGATGATTCCAGTAATAAGCCTTATTGGTATGGTTCAC530.10629763337344565No Hit
TTTTCTGGGGGAAGTGCGGAGCGGGTGGGCCTATATAGATGTTGAGGTGCGG530.10629763337344565No Hit
ATCTTTGGGCGATCTTGTCGAGGGGCTCTTTCCCATCTTGCAAGATGGCGGG530.10629763337344565No Hit
GCTCGCGGGAATATATTTGAGATTCATGGAAGGTTCATAACTCACTTATTAG530.10629763337344565No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAA530.10629763337344565No Hit
TACCTCTGGGCTTGATCTTTGTGACTTGTGCTTTTTTAGCTTCTCTCTTGAA530.10629763337344565No Hit
TTGACAGGGACTCCATGTGCTCCCTCTGCCAGGAGGAGAATGAAGACATGGT530.10629763337344565No Hit
CAATCAGGGAGGCTATACCATTTTCTCCAAAGGCTGAATATTATGAAAAACA530.10629763337344565No Hit
ATGCTAGGGGCTCTCTCTTTCCCTTCGGTGTGCCACTGAAGATCCTGGTGTC530.10629763337344565No Hit
GCCCGGAGTTCAGATCTTCTCTGTTCATGGTCTCCAGAGGGAAATGTGTGTC530.10629763337344565No Hit
TAGAGGGGGTCAGAGAGGGTGACAAGATAGAGAGCTATTTAATGGCCGGCTG520.10429201764941838No Hit
ACAAGTGGGACATTTACATTTCAGGCCTCTGAAAGGCAGAATCCTCAAATTT520.10429201764941838No Hit
ACATTCGGGAGTGGAGGAGATGCCCATGAATGTGGCTGACCTTATTTAATTC520.10429201764941838No Hit
GGTGCAGGGCAGCATAAAGGATGCCCATGCAAAAATCCCATCCAAAGGTCAC520.10429201764941838No Hit
GAACAGGGGAGGCCGGCACTTCTGAGTGACAGGTGCCACCTGCGTGTGTCTT520.10429201764941838No Hit
GCTCATGGGAATGACCCTGAGAACATGGAGAAGCGCAGGAGCCGTGAGAGCC520.10429201764941838No Hit
TAGCTGGGGCAGTGACTACAGGATGTGGCTGACAGTGCTCACTGAAAGGAGA520.10429201764941838No Hit
GCGCTAGGGGACTAGGTGTGTCCCATCCAAGTTGAGCACGCGCCTTCCCCAG520.10429201764941838No Hit
TAAGCAGGGGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTACTGGG520.10429201764941838No Hit
ACAAAAGGGGATCTATCTGCGGGGCCTTTCGGCCGGAACCGCCATCTTCCAG510.1022864019253911No Hit
TTCTATGGGACAAAAGTTAAATGGAACATTATAATTATTTTAAGTAATGTTA510.1022864019253911No Hit
TCGCATGGTCTCTTTTCCAGTATTAAACACTCATATGCTTATGGCTTGGAGA510.1022864019253911No Hit
CGTCTAGGGATCGACAGCAGGAATTAATAAGTTTGAAGAAAGGAAAAAATAG510.1022864019253911No Hit
TACTGCGGGATGCTACTTGTACCAAGGCACCCATTCATATTCAGGACTGTTA510.1022864019253911No Hit
GTAGAAGGGGGCTGCATAACAGCTTGAAAACTGGATTATCTACTACAGAGTG510.1022864019253911No Hit
ACTGTCGGGCTTACAACTACTATTTAAAGTCAGCAACTTTTCACTGAATTTG510.1022864019253911No Hit
AAAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA510.1022864019253911No Hit
AGCACTGGGGGCCACGTGGTTCAACCAGCCGGCCCGTAAGATCCGCAGACGT510.1022864019253911No Hit
TCCGCTGGGGAAGTAGAGTGGATCATCACAAACCGGAAAGGTTTTCTTTTAT510.1022864019253911No Hit
GACATAGGGGAAAGCCTCAGAGTCAATGGGAAGTCATGATTCAGTTTTCAAA510.1022864019253911No Hit
GAAACCGGGACTGTCATGTTTTCAAAGTTGACATGGAAAATTTTACATGAAG500.10028078620136383No Hit
GGCTTGGGACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAG500.10028078620136383No Hit
TCGCTTGGGTTTCTAAATATTAATAGGGGGGCTTGATTCAACAAAGCCACAG500.10028078620136383No Hit
GGGAATGGGATCTTGAATGTATAATCCTTTTTGTAACCCAGGTTGGTTTCTA500.10028078620136383No Hit
TACCCAGGGAGGGGTTGTATCGGTTGATTTCAGCATACCACAGCTGTAATTT500.10028078620136383No Hit
CTTCGGGGAGCAGGGGGACAGAAAAAGTAACAAATTTGAAATATACCCAGAG500.10028078620136383No Hit
CACGCGGGGTAATCGTTCTTTCATGGTGCTATTGTAGTATTTGGTTTGCCTT500.10028078620136383No Hit
TGTAACGGGATTCTTCTGAGAAGTTGGGGCAGAATTGGCAAGTCATTTATAT500.10028078620136383No Hit
TGCAAGGGCCCAGAAGAGGTTCACCTGCCAACAGGCCTTGCGGCACCTTTGG500.10028078620136383No Hit
ATGTTTGGGATACGCGGGGATACAAAGATGAATTACAAACAGTTCTTGAGAT500.10028078620136383No Hit
GTATCTGGGGACATATGAGAAGTCTGTACTCTCTGCTCCGTTTAGCTGTGAA500.10028078620136383No Hit
TCAACGCGGGAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAA500.10028078620136383No Hit
CTTCCAGGGGTTTTTTTTCTTTGGAGTTTTTGTGGTATTAAATTATTTTTAA500.10028078620136383No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)