FastQCFastQC Report
Sat 31 Dec 2022
SRR5848920.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848920.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49712
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC4250.8549243643385903No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT3590.7221596395236562No Hit
TCTCTGGGGATGGTGGGCTTTGCGGGAGAGAAGGGCACAGACTGGCTCCCCT1030.20719343418088187No Hit
TTTCTAGGGAACGGGCCTAGGGAATAGAAGCATGGTGAAAGGGGAATGGAGA1010.20317026070164143No Hit
GCGTGAGGGAACCAGGGAGGTAAAAGATCTTTACAGTGAGAACTGCAAAACA850.17098487286771805No Hit
CCATAAGGGGAGGCGCTGAGGCTGCTGGAGGCGCGGGCCGGGCGGTGCGCAC840.16897328612809784No Hit
GTTGTCGGGCATTTAATTAAGATTTAATCATTTGCTAATGGAAATCTTACCA830.16696169938847763No Hit
GTAAACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC820.16495011264885742No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAA790.1589153524299968No Hit
GAAATAGGGATTTATTTACTTTTTCATACTTTTTTCCTTTCTATTACATTGG790.1589153524299968No Hit
TTGACAGGGGATCTTATTCAGGGATAGTGTGGGTGTCTATGTAATTACTAAA770.15489217895075635No Hit
TCAACGCGGGAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAA750.15086900547151594No Hit
GTGTTGGGGAAGGCGGACGACTAGAGTCGTTGGGCCCGGCGCGACCCGCAGG740.1488574187318957No Hit
TTTGTTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT740.1488574187318957No Hit
ACTTTTGGGAGTACAATTAGAATACTTTAATCTCTTTGAGATGCTGAAAATA740.1488574187318957No Hit
TGAGACGGGATCAGCTTAAAAAAACCCTTCTATGAAAACTTTGAGAGAAAAT730.14684583199227552No Hit
TCGCATGGTCTCTTTTCCAGTATTAAACACTCATATGCTTATGGCTTGGAGA720.14483424525265529No Hit
TGATCCGGGGATCTGATGACATGGGCTTTTTAAAAGATTACTATTTTTTTTA720.14483424525265529No Hit
GTAACAGGGAAGCCCAAACCTGTGGTGTCCAAAGAGAAACTAGTCAGAAAAC710.14282265851303508No Hit
AGTCACGGGGGCTGACGACTATGAACAGGTCAAGAACGTGGCCGATTACTAC710.14282265851303508No Hit
GCCAAGGGGCTGTAGAGTATTCCATGGTATATATGTACCATATTTTCTTTAT700.14081107177341487No Hit
TGCGCGCGGGGAGCATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTC690.13879948503379466No Hit
TTGCTAGGGCTGAATTCTGCCAGAGGTACAAAGAAGACCTTCAAACAATACT680.13678789829417443No Hit
TATGCACTAGGGCATGAGCCACAGCCACCACACTCTGTCCTCAAGTTCCTGC680.13678789829417443No Hit
GATTTTGGGAAGTTGAGGACTGTCTACACAATGGATTGAAGGGAGGGCAAGA680.13678789829417443No Hit
TGTGGCGGGATGAGCAGTGGGAGTGCAGCGCCAGGTGACAAGATGTTGTGAG670.13477631155455425No Hit
ACTAATGGGGACTCACTCGCGCGTTAGGAGGCTCGGGTCGTTGTGGTGCGCT670.13477631155455425No Hit
CTTTTAGGGCGTTACCATGACTCTGACACTTCTAATCATTTGGTCTTGAGAA670.13477631155455425No Hit
ACACGTGGGGAAGAAAAGACCGGGATCAGCAAAACATGAAATCGTTATAGCA670.13477631155455425No Hit
TAAGGTGGGGCCGAGGGCAGTGGTTGGCTTTTGCGTTGCAGATTGCAGGGTA660.132764724814934No Hit
TCGTTGGGGCACAGTGGGGAGAAACCCTATGAATGTAATGAATGTGGGAATG650.1307531380753138No Hit
TGAGTTGGGACCATAAAGGAAACATTTTATCTCTTCTGTGTCAGTCTGTGAC650.1307531380753138No Hit
ATGCCAGGGCACACTGGGTGTTAGCTTTCAACATAATCGGGGGGCGAGGAGA640.1287415513356936No Hit
GACATAGGGGAAAGCCTCAGAGTCAATGGGAAGTCATGATTCAGTTTTCAAA640.1287415513356936No Hit
CTCTTAGAGAGGGAATTGGGAAGAAATCTGGTATCCAAGCTTAAATTTCTTG630.12672996459607339No Hit
TAGGTGGGGACCAGTCACTTTGTAGCTTTGCAGTGGTTCAGCAATTAGCCAT630.12672996459607339No Hit
ATCTATAGGATTTTTTGTTGCGGAAAGCTATCTTGTGCATTGTAGATGTTTA630.12672996459607339No Hit
TATGGCGGGTAGCACGGGCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGG620.12471837785645316No Hit
CAATGTGGGACAGAAATTTGAGGAGCCAGATATTGTAGAAGATGGTATGTGT620.12471837785645316No Hit
CACGCGGGGTAATCGTTCTTTCATGGTGCTATTGTAGTATTTGGTTTGCCTT620.12471837785645316No Hit
ACTGTAGGGCAAACTGAGAAACGTAATGGGCTAGTAAGATGGATGGAAAAGA620.12471837785645316No Hit
GCGGCCGGGTGTAGGGACCTGCTTAGTTCCTGAAAAGGGGAGTGTAACTAGT620.12471837785645316No Hit
CTCACCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA620.12471837785645316No Hit
CGATATGGGGGGAAGGCCAATCAATGGAGCAGTCAGAACACACACATCTATC620.12471837785645316No Hit
GCCCGGAGTTCAGATCTTCTCTGTTCATGGTCTCCAGAGGGAAATGTGTGTC620.12471837785645316No Hit
TTGAGAGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC620.12471837785645316No Hit
TAGCATGGGAGAAGGACTTGCTGAATACATTGTTGAGTTTCTGAAGAAGAAG610.12270679111683297No Hit
TTTTCTGGGGGAAGTGCGGAGCGGGTGGGCCTATATAGATGTTGAGGTGCGG600.12069520437721275No Hit
CTAGATGCGGGTTGCCTATTCAATAGTTCATTCTTTTTTATTACCAAATAAT600.12069520437721275No Hit
ACAAGTGGGACATTTACATTTCAGGCCTCTGAAAGGCAGAATCCTCAAATTT600.12069520437721275No Hit
TAAACAAGGGAGGTGAAAAATTCACAGAACAAAAATGTCGATTATTTTAAAA600.12069520437721275No Hit
TGTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA600.12069520437721275No Hit
ACATACGGGGTATTTTACCTCTCAGTTTCGGTGTGGTCACTTACTATCTTTA590.11868361763759253No Hit
ATCTTTGGGCGATCTTGTCGAGGGGCTCTTTCCCATCTTGCAAGATGGCGGG590.11868361763759253No Hit
ATTTTGGGGCAGCAAAATAGAACAACTTATAACTGGATGACCCTCAAAAACC590.11868361763759253No Hit
ACCTAGGGGGATGGGGAACAGAAAGGGAGTATGGAAAGAAAAATTATAAGAG590.11868361763759253No Hit
CTTAACGGGATCATGAGGGAATTCCGGTCAGGGTCAAGTCGTGTTCTGATCA590.11868361763759253No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCTGTC580.11667203089797233No Hit
TTAGTTGGGCAACTAATTTCTTTTTTAGTATTTAATTTTGAAATAATTTCAG580.11667203089797233No Hit
GCAACAGGGAAGATAGACGCATAGTAGTTTATGTTCTCAGTCATCTTATTAC580.11667203089797233No Hit
ACATGTGGGGGAGCCTGGCCCAGGAGAACCAGAGATGATCCCGAGGCAGCTG580.11667203089797233No Hit
CGTGTTGGGACAGAACCTGTGATTATTCTCGCATGATAAGGATGGTACTTGC570.11466044415835211No Hit
TACGTTGGGTGTGGGGGATGTTCTCCGTTATTACCAGTTACATCCTCTTCAG570.11466044415835211No Hit
ACCTTAGGGATCCCACTTGTGATTTCTTTGTTTTGGGTCAAGAAAAGCTTTT560.11264885741873189No Hit
ACGCTGGGGATTATTTTCTGTGAAATAAAAATGCAGCACTGCATTTTTTTAA560.11264885741873189No Hit
GTCCATGGGATCCTCCTGCTTGCAACTATAGCAACAGCCTTCATAGGCTATG560.11264885741873189No Hit
GACACAGGGGTATATCATGATATGCCTATCATATCATATGCATATGATAACC560.11264885741873189No Hit
AATACGGGTCTGCAGACTCTGGAGGAGCAGGTGTCTGGTGAGCGACAGCGCC560.11264885741873189No Hit
GGCTTGGGACCGAACACCATGCCTTCAATTAAGTTGCAGAGTTCTGATGGAG550.11063727067911168No Hit
CTATGGAGGACTTCTATTATGAAGAAAAGGATGATGATGATACTAGTAAATG550.11063727067911168No Hit
TTGTCTGGGGAAACACTACCAAGAGAATGAAGAGACAAGCCAAAGAACGGGA550.11063727067911168No Hit
GTCTATGGGAGTACTTCTTCAGGGAAACCTGAAATTTCTAATGCCTTGAAAA550.11063727067911168No Hit
AAAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA550.11063727067911168No Hit
TAGCTGGGGCAGTGACTACAGGATGTGGCTGACAGTGCTCACTGAAAGGAGA550.11063727067911168No Hit
TAACTTGGGATATTTCTGTTACTGTGGCCCAAGGACAGCTAACTGGCTATGT550.11063727067911168No Hit
AGTATAGGGCATCTCCCTGACCTTACAGAGAGAATCTGATGGCTCTGCATCC550.11063727067911168No Hit
TATCACGGGTCCTCTTTGTTTATCTCTGAGACAGTCCAACCTTGAGAATAGC550.11063727067911168No Hit
GAAACAGGGGATCTTACCAAGGGACACTACATTGTACAAGGAATGAAAGTGT540.10862568393949147No Hit
AGTAAAGGGCAGGTACGACATGTATAGGTTACCTAGATGTTATGAGAAATTT540.10862568393949147No Hit
ACATACGGGTCTCATACGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTT540.10862568393949147No Hit
TTGACAGGGACTCCATGTGCTCCCTCTGCCAGGAGGAGAATGAAGACATGGT540.10862568393949147No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAAACTCGTATGC540.10862568393949147No Hit
CCGTCCGGGATGGCAGAGTCAGATCTCTAACATCTTTGACTCCTAGGCTAAT540.10862568393949147No Hit
AACGACGGGGACTCCTACCCCTCACAATCATGGCAAGCCAACGCCACTTATC540.10862568393949147No Hit
AAGAAAGGGAGACCCACACATACATGGTCAAATGATTTCGACAAGGACACCT540.10862568393949147No Hit
AGCCGAGGGACAAGCAAGACTGGGACCTTAGAAATCATTGTAGAAACACAGT530.10661409719987125No Hit
TTTTATGGGGGAATAACATAACTTTTACTGTCCTATACTTAAAAAAATCTGT530.10661409719987125No Hit
TGACCAGGGGGGTCCACCACAGTGAATTTGTAACTTTGAAGTCAGGATAGAA530.10661409719987125No Hit
TGCTACGGGAGGATGAATGATAAAACAAGTACAAGAATATCAATCATATTTT530.10661409719987125No Hit
CACTGCGGGGCTCGGGCCAGCATTCGGATAGCCACGGGGAGTCATCAGCCGA530.10661409719987125No Hit
TATTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA520.10460251046025104No Hit
GTTGGTGGGGACCAAGATGATTCCAGTAATAAGCCTTATTGGTATGGTTCAC520.10460251046025104No Hit
GCGACCGGGAGTGCGTTACTTACCTCGACTCTTAGCTTGTCGGGGACGGTAA520.10460251046025104No Hit
TACTGCGGGATGCTACTTGTACCAAGGCACCCATTCATATTCAGGACTGTTA520.10460251046025104No Hit
CGACTAGGGAGCATTTCAATTCTTAGGTATATACACAACCAATAAACATACA520.10460251046025104No Hit
GTGATGGGGGAGCATTACCCCTACTGCGGGTCCCGCTGCTGGCAGCGCTGGA520.10460251046025104No Hit
CAATCAGGGAGGCTATACCATTTTCTCCAAAGGCTGAATATTATGAAAAACA520.10460251046025104No Hit
CTTATCGGGGATGAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATT520.10460251046025104No Hit
TCACTTGGGATCTGGGATGTTATGGGAAATGAAGAGCTCTGTGATTTTGTAA520.10460251046025104No Hit
TGGCTTGGGAGAGTTATTGATGATGGTTAAATGAGATAATCGGAGAAGAGTG510.10259092372063083No Hit
ATGTCAGGGTAGTTATTTGTTTTCCCTGTCAGTAGGTTGCCTTTTCGTTTTG510.10259092372063083No Hit
TTTCTGGGAGCTTGATGAGGATAGCATTGAATCTGTACATTACTTTGGGCAG510.10259092372063083No Hit
AGCGCAGGGGGAGGCTTTGCAGGAAACTCTCATTCGTGCACTAAGGACCTTA510.10259092372063083No Hit
ACCTACGGGCTCAAATCTCTGCTGGGCAACTAACTAGCTGTGTGACTTTGGG510.10259092372063083No Hit
CTAACTGGGGCAGTTCTGTGACTTCCAAGCCAGGAATTAAATGCTCTTTTTA510.10259092372063083No Hit
AGCTGAGGGACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTA510.10259092372063083No Hit
CATTTGGGGATGTACGTTACCTCCATCTATTTTAGGATATTTTCCTCACCTA510.10259092372063083No Hit
AGCATAGGGAATTTCTATAGATTAAAAACTATTAATAGTAGTAATTGCTAAA510.10259092372063083No Hit
TGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCAGCCAACACTAGTCATTT500.10057933698101063No Hit
TTGTAGGGGGTCTGCTACGGGGTTATAAAATTTAAGCAATTGTTAGTGTTTA500.10057933698101063No Hit
CGTCTAGGGATCGACAGCAGGAATTAATAAGTTTGAAGAAAGGAAAAAATAG500.10057933698101063No Hit
ACTACTGGGAAGACATAAGTCAAAATTTATAATTTTGTAAGACTTCAAACTT500.10057933698101063No Hit
GTCATGGGGATATGTTGAGAGAGATTCTTCGGTATAAACTAGACCCTTATTA500.10057933698101063No Hit
AGCCAGGGAGCTCACTGCAACCTCCATCTCCTGGGTTCAAGTGATTCTCGCA500.10057933698101063No Hit
CTAGGTGGGAAGCAATACACACCTACCTGGAAAATCGTGTTGAGTCTGTGGG500.10057933698101063No Hit
GCACCGGGCCCGAGTCTGTTCTCTATCACATCTGAGGGGGTCTTCCAGAATT500.10057933698101063No Hit
TTTATCGGGCCGATCTCTGGAGGGTAGTCGGGGGCATGACTAGTAAGATAAC500.10057933698101063No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAA500.10057933698101063No Hit
CTCACAGGGGGGCCTGGGGAAGCAGCCTGACCAAGGCGCTCAGGTGTGCCTG500.10057933698101063No Hit
AAACTAGGGCGCCAGGGGATAGTTTTCCTTTTTGTATGTACCTATAAACGGA500.10057933698101063No Hit
ACGATAGGGGTCCCAGCTAGCTCTGTTACCAGCCTTTTGGTGTGTCTTTATG500.10057933698101063No Hit
ATGTTTGGGATACGCGGGGATACAAAGATGAATTACAAACAGTTCTTGAGAT500.10057933698101063No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)