FastQCFastQC Report
Sat 31 Dec 2022
SRR5848919.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848919.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49058
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT4290.874475111092992No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC3800.774593338497289No Hit
AGTCACGGGGGCTGACGACTATGAACAGGTCAAGAACGTGGCCGATTACTAC1010.20587875575848996No Hit
TTTCTAGGGAACGGGCCTAGGGAATAGAAGCATGGTGAAAGGGGAATGGAGA880.17937950996779323No Hit
TCTCTGGGGATGGTGGGCTTTGCGGGAGAGAAGGGCACAGACTGGCTCCCCT880.17937950996779323No Hit
TTTGTTGGGAGTCCGAGTGGAGAGAGCGAGCTGAGTGGTTGTGTGGTCGCGT880.17937950996779323No Hit
CTTTTAGGGCGTTACCATGACTCTGACACTTCTAATCATTTGGTCTTGAGAA870.17734110644543194No Hit
ACTTTTGGGAGTACAATTAGAATACTTTAATCTCTTTGAGATGCTGAAAATA850.17326429940070937No Hit
CGATATGGGGGGAAGGCCAATCAATGGAGCAGTCAGAACACACACATCTATC790.16103387826654164No Hit
GCGTGAGGGAACCAGGGAGGTAAAAGATCTTTACAGTGAGAACTGCAAAACA780.15899547474418035No Hit
TATGGCGGGTAGCACGGGCACTGGGTCAAGTGACTGCTTCCTCAAACTTTGG780.15899547474418035No Hit
GTAAACGGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCC720.14676505361001263No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAA710.14472665008765134No Hit
TAACTTGGGATATTTCTGTTACTGTGGCCCAAGGACAGCTAACTGGCTATGT710.14472665008765134No Hit
TGCGCGCGGGGAGCATAGTACCTCCAGTTGTCAGTAACTGCATTCACTTCTC700.14268824656529006No Hit
GTTGGTGGGGACCAAGATGATTCCAGTAATAAGCCTTATTGGTATGGTTCAC690.1406498430429288No Hit
TAGGTGGGGACCAGTCACTTTGTAGCTTTGCAGTGGTTCAGCAATTAGCCAT690.1406498430429288No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAAACTCGTATGC690.1406498430429288No Hit
GCGCTAGGGGACTAGGTGTGTCCCATCCAAGTTGAGCACGCGCCTTCCCCAG680.1386114395205675No Hit
TCGTTGGGGCACAGTGGGGAGAAACCCTATGAATGTAATGAATGTGGGAATG680.1386114395205675No Hit
AGACAGGGGCTGGTTGGAGAGGAGAGAGAAGAGGCTCAGAGACAAGTAAGAG670.1365730359982062No Hit
TTGACAGGGACTCCATGTGCTCCCTCTGCCAGGAGGAGAATGAAGACATGGT670.1365730359982062No Hit
ATCTATAGGATTTTTTGTTGCGGAAAGCTATCTTGTGCATTGTAGATGTTTA670.1365730359982062No Hit
GTTGTCGGGCATTTAATTAAGATTTAATCATTTGCTAATGGAAATCTTACCA660.1345346324758449No Hit
ACTGTAGGGCAAACTGAGAAACGTAATGGGCTAGTAAGATGGATGGAAAAGA660.1345346324758449No Hit
ACCTAGGGGGATGGGGAACAGAAAGGGAGTATGGAAAGAAAAATTATAAGAG660.1345346324758449No Hit
TGAGACGGGATCAGCTTAAAAAAACCCTTCTATGAAAACTTTGAGAGAAAAT650.13249622895348362No Hit
ACTACTGGGAAGACATAAGTCAAAATTTATAATTTTGTAAGACTTCAAACTT650.13249622895348362No Hit
AATTTGGGGATTTTCAAATTGTATATCTGATAAGGGACTTGTGTCTACAACA650.13249622895348362No Hit
TAGCATGGGAGAAGGACTTGCTGAATACATTGTTGAGTTTCTGAAGAAGAAG640.13045782543112233No Hit
TGTGGCGGGATGAGCAGTGGGAGTGCAGCGCCAGGTGACAAGATGTTGTGAG630.12841942190876107No Hit
ACATACGGGGTATTTTACCTCTCAGTTTCGGTGTGGTCACTTACTATCTTTA620.12638101838639978No Hit
TGATCCGGGGATCTGATGACATGGGCTTTTTAAAAGATTACTATTTTTTTTA620.12638101838639978No Hit
ATTTTGGGGCAGCAAAATAGAACAACTTATAACTGGATGACCCTCAAAAACC610.12434261486403848No Hit
TAGCTGGGGCAGTGACTACAGGATGTGGCTGACAGTGCTCACTGAAAGGAGA610.12434261486403848No Hit
GGGAATGGGATCTTGAATGTATAATCCTTTTTGTAACCCAGGTTGGTTTCTA600.1223042113416772No Hit
TACTGCGGGATGCTACTTGTACCAAGGCACCCATTCATATTCAGGACTGTTA600.1223042113416772No Hit
ACATGTGGGGGAGCCTGGCCCAGGAGAACCAGAGATGATCCCGAGGCAGCTG600.1223042113416772No Hit
TCGATGGGCTTATTCTAATAGTTTGTTAATTTTCTTTTCAAAGAACAAACTT600.1223042113416772No Hit
TACGTTGGGTGTGGGGGATGTTCTCCGTTATTACCAGTTACATCCTCTTCAG600.1223042113416772No Hit
TAAGGTGGGGCCGAGGGCAGTGGTTGGCTTTTGCGTTGCAGATTGCAGGGTA600.1223042113416772No Hit
TTGAGAGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC600.1223042113416772No Hit
TTGCTAGGGCTGAATTCTGCCAGAGGTACAAAGAAGACCTTCAAACAATACT590.12026580781931591No Hit
CTATGGAGGACTTCTATTATGAAGAAAAGGATGATGATGATACTAGTAAATG590.12026580781931591No Hit
CGTCTAGGGATCGACAGCAGGAATTAATAAGTTTGAAGAAAGGAAAAAATAG590.12026580781931591No Hit
TTGTCTGGGGAAACACTACCAAGAGAATGAAGAGACAAGCCAAAGAACGGGA590.12026580781931591No Hit
TCGAACGGGGAGAATGGGAGTTGACCCCAAGGTGAGAGGAGTGAAACCACTT590.12026580781931591No Hit
TGGTTAGGGCTGCTTTACCACTTCTAGTCAGCATTTGATGGAGGGTATTAAT580.11822740429695462No Hit
TATGCACTAGGGCATGAGCCACAGCCACCACACTCTGTCCTCAAGTTCCTGC580.11822740429695462No Hit
TATCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC580.11822740429695462No Hit
ACATACGGGTCTCATACGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTT580.11822740429695462No Hit
CCATAAGGGGAGGCGCTGAGGCTGCTGGAGGCGCGGGCCGGGCGGTGCGCAC580.11822740429695462No Hit
GCGGCCGGGTGTAGGGACCTGCTTAGTTCCTGAAAAGGGGAGTGTAACTAGT580.11822740429695462No Hit
ATCTTTGGGCGATCTTGTCGAGGGGCTCTTTCCCATCTTGCAAGATGGCGGG570.11618900077459333No Hit
CAATGTGGGACAGAAATTTGAGGAGCCAGATATTGTAGAAGATGGTATGTGT570.11618900077459333No Hit
CCGTCCGGGATGGCAGAGTCAGATCTCTAACATCTTTGACTCCTAGGCTAAT570.11618900077459333No Hit
AGCTGAGGGACGAACGCTCCAACGAGTACCAGCTGATTGACTGTGCCCAGTA570.11618900077459333No Hit
GCCAAGGGGCTGTAGAGTATTCCATGGTATATATGTACCATATTTTCTTTAT570.11618900077459333No Hit
GAAATAGGGATTTATTTACTTTTTCATACTTTTTTCCTTTCTATTACATTGG570.11618900077459333No Hit
AGCCGAGGGACAAGCAAGACTGGGACCTTAGAAATCATTGTAGAAACACAGT560.11415059725223206No Hit
TCACTTGGGACTGGAGCTTAAGAACATACTTAGTTTCTCATGTGAAAACTTA560.11415059725223206No Hit
AGTATAGGGCATCTCCCTGACCTTACAGAGAGAATCTGATGGCTCTGCATCC560.11415059725223206No Hit
TTGACAGGGGATCTTATTCAGGGATAGTGTGGGTGTCTATGTAATTACTAAA560.11415059725223206No Hit
TATACTGGGATACCAGTCCTTTAGAAGTCCTTTGTCTCATATGTAATTTGAA550.11211219372987077No Hit
GAGCATGGGGTGTGCCTGTGTTCTTACTACCATCACAGCTGAATGCAATGAA550.11211219372987077No Hit
TGACCAGGGGGGTCCACCACAGTGAATTTGTAACTTTGAAGTCAGGATAGAA550.11211219372987077No Hit
TGATAGGGGGCGCAGCCGCGGTGGGAGGAGGCACCGGCCGCGCGGCGGGAGG540.11007379020750949No Hit
TAAACAAGGGAGGTGAAAAATTCACAGAACAAAAATGTCGATTATTTTAAAA540.11007379020750949No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCTGTC540.11007379020750949No Hit
AAAAACGGGGTTCTCAATGTTGTCATATTGTCTTTGATTCATGCCTTTGAAC540.11007379020750949No Hit
TAGCGCGGGGGCCACAGTGAACCAACCAAACCAACCCCGAATCATCGCTCTC540.11007379020750949No Hit
ACTGTCGGGCTTACAACTACTATTTAAAGTCAGCAACTTTTCACTGAATTTG540.11007379020750949No Hit
ACTAATGGGGACTCACTCGCGCGTTAGGAGGCTCGGGTCGTTGTGGTGCGCT540.11007379020750949No Hit
TAGTAGGGGATGAGTGGCCTTAATAAATATAGTTGTCTTTCTTTGCTGCTGT540.11007379020750949No Hit
ACTGCAGGGAGAGCTAACTTGAAGAAATGCCCAAAGATGTAACAATTTGAGC540.11007379020750949No Hit
AATACGGGTCTGCAGACTCTGGAGGAGCAGGTGTCTGGTGAGCGACAGCGCC540.11007379020750949No Hit
TCAACGCGGGAAGGAAGGATGATTTTCAGATGTATGCAAAATATTGTCAGAA540.11007379020750949No Hit
TCGCTTGGGTTTCTAAATATTAATAGGGGGGCTTGATTCAACAAAGCCACAG530.10803538668514819No Hit
ATTGCGGGGAAATATGAGACGACATACATAAACTAAGAAACAAAAATATTGC530.10803538668514819No Hit
GGCTGGGAGCACAAACAAAACTTAAATCAGGGTACATGACATGAAGTATGTA530.10803538668514819No Hit
GCAACAGGGAAGATAGACGCATAGTAGTTTATGTTCTCAGTCATCTTATTAC530.10803538668514819No Hit
GCAAAAGGGATTCCAATGTTCAGTGGCGGAACTTGCAATCCTCAGTTTGTGG530.10803538668514819No Hit
ATGCCAGGGCACACTGGGTGTTAGCTTTCAACATAATCGGGGGGCGAGGAGA530.10803538668514819No Hit
CATGATGGGGACTCCTGGATCTATGTGTATGCAGAAGGCTACATATACATAG530.10803538668514819No Hit
CAAATAGGGAGACCTAATTTGCAAACTCAATCGGGGACTAAAATTTCCCACT530.10803538668514819No Hit
CGTGTTGGGACAGAACCTGTGATTATTCTCGCATGATAAGGATGGTACTTGC520.1059969831627869No Hit
TGTGCTGGGATTACAGGCGTGAGCCACTGCGCCCAGCCAACACTAGTCATTT520.1059969831627869No Hit
CACGATTGGGTTCTCCAGAAAATGAAACTGCTGATAACAGTTTTTCTCAGGA520.1059969831627869No Hit
CTCTTAGAGAGGGAATTGGGAAGAAATCTGGTATCCAAGCTTAAATTTCTTG520.1059969831627869No Hit
ACTAGTGGGGCTCTTTCCGTCTCAGGTCGCCGCTGCGAAGGGAGCCGCCGCC520.1059969831627869No Hit
CTATACGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG520.1059969831627869No Hit
GCACCGGGCCCGAGTCTGTTCTCTATCACATCTGAGGGGGTCTTCCAGAATT520.1059969831627869No Hit
GATTTTGGGAAGTTGAGGACTGTCTACACAATGGATTGAAGGGAGGGCAAGA520.1059969831627869No Hit
GTAACTGGGAAAAGAGTCAGTGTAAGGAAATTCTGGTGGGAAGGTGATGGAA520.1059969831627869No Hit
ATTGACGGGCTATCACCATGGGGATGGTTCTTCTAAATGAAGCAGCAACTTC520.1059969831627869No Hit
CTAACTGGGGCAGTTCTGTGACTTCCAAGCCAGGAATTAAATGCTCTTTTTA520.1059969831627869No Hit
GAAATGGGGGTACCTGTAAATTACATCATTTTCAGGATATCCCTTGGAATAC520.1059969831627869No Hit
ACACGTGGGGAAGAAAAGACCGGGATCAGCAAAACATGAAATCGTTATAGCA520.1059969831627869No Hit
TAAGCAGGGGACCTCAGGTGATCTGCCTGCCTCAGCCTCCCAAAGTACTGGG520.1059969831627869No Hit
GCCCGGAGTTCAGATCTTCTCTGTTCATGGTCTCCAGAGGGAAATGTGTGTC520.1059969831627869No Hit
ACGCTGGGGGAGTAGAAAAATGCAAATTTGCCTTATCTCTAAAGAATAAATC510.10395857964042561No Hit
CACGCGGGGTAATCGTTCTTTCATGGTGCTATTGTAGTATTTGGTTTGCCTT510.10395857964042561No Hit
GTAAGCCGGGTAGGTTGTAAGACCAACTTTGGATTGGATCTAGCCAGTGAAT510.10395857964042561No Hit
TCCTATGGGATTGCATGACGGGAGCACTAGGTGAAGCCACGGAAATTGGATT500.10192017611806432No Hit
GCGTATGGGCATGGTCTTATATCACCGTATGAAATGGAAACTTACAGGACTT500.10192017611806432No Hit
CTGAATGGGAGCTTTGTTGATTTCCACAAAAATGTATTAGATCTTAATTAGT500.10192017611806432No Hit
ATTCGCGGGGAGATGGCCATGTCTCCTTGCAAGAATACATGGCTTTCATGAT500.10192017611806432No Hit
CATAGCGGGAGTCTACACGGGAGTGTCTGATGCAACTTTGAATTATGCAAAT500.10192017611806432No Hit
ACGTTCGGGATGGGCAATGAGATCGATACACAGAATCGCCAGATCGACAGGA500.10192017611806432No Hit
CGACTAGGGAGCATTTCAATTCTTAGGTATATACACAACCAATAAACATACA500.10192017611806432No Hit
AGCACTGGGGGCCACGTGGTTCAACCAGCCGGCCCGTAAGATCCGCAGACGT500.10192017611806432No Hit
ATAACGGGTCTACATCATTCCTTAAGTAAGGAAAGTCAGATACTGACATTTT500.10192017611806432No Hit
CTTATCGGGGATGAGAAGAGCCTTGCTTTATTACTCAATTATCTTCACGATT500.10192017611806432No Hit
TACAACGGAACACTAGACTGAATCTCCTTTTTGGAGTATGTATCTTCTCTCA500.10192017611806432No Hit
CTTAATGGGGAACCCGTTGTAACACAACCGAAACCAAAAATTGAATCACCCA500.10192017611806432No Hit
AACTGAGGGGACCTCTTTTTAAGGACACTGGGGTCTGTTTCTGGAAATGTTC500.10192017611806432No Hit
CATTTGGGGATGTACGTTACCTCCATCTATTTTAGGATATTTTCCTCACCTA500.10192017611806432No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)