FastQCFastQC Report
Tue 3 Jan 2023
SRR5848916.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848916.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189568
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC9670.5101071910871032No Hit
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT8990.4742361580013505No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTCG206.2697916E-446.0419851
CGCATAG206.2697916E-446.0419851
CGTTGAG253.3879704E-546.0419851
CTGAACG206.2697916E-446.0419851
CTAGTCG253.3879704E-546.0419851
CTACGTA206.2697916E-446.0419851
GTGTACG253.3879704E-546.0419851
TATGTCG206.2697916E-446.0419851
GCGTATG405.524271E-946.0419851
TCGCCAA206.294318E-446.00554343
CTATGAC206.294318E-446.00554324
CGGAACA206.294318E-446.00554324
AACCGCC206.294318E-446.00554323
GTACCGA206.294318E-446.00554322
ACGCCTA253.403866E-546.00554315
GTCGAGT206.294318E-446.00554343
CGAGTCC206.294318E-446.00554345
GTTTCGT206.294318E-446.00554326
CCCTATA206.294318E-446.00554319
ACGTTAA206.294318E-446.00554325
Produced by FastQC (version 0.11.5)