FastQCFastQC Report
Sat 31 Dec 2022
SRR5848914.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848914.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences141307
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGCCGTCT7730.5470358864033629No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACGACACAAAATCTCGTATGC6860.4854678112195433No Hit
AGTACAGGGATCTCACAAATTTAAAATTTTCACTTCATATTTTTAATTCATC2010.14224348404537637No Hit
GCATCAGGGTATGATCAGAAAACAGAAGCTTTTGAACCAAACATTTATACTA1720.1217207923174365No Hit
TTTGTTAGGGACATGGGAGAATTTTAAAACATGCGCTTTTAGAATCAAAATT1710.12101311329233513No Hit
GCTTTCGGGAGATCTTGCCCTCAAAGAGTTTATAGTCTACTGGAGAAAGGGT1690.11959775524213237No Hit
GAGGCTGGGAGCCCCTGCTGCTTCCCCCGTCTCCGCCTGGGGGAGCCCACCG1680.118890076217031No Hit
CCTTGGGGGAATCCCAGCACTTTGGGAGGTTGAGGCAGGAGGATCACTTGAG1610.11393632304132137No Hit
GTAAACGGGTCCGATCTGCTCTGGGACAATAACAAAATTTCTAAGATTTCTA1580.11181328596601725No Hit
GTCTAGGGGCAGGACACGGGTCGCTGGCGGGTTAGGTGGCTGCGTGGCCGCC1550.10969024889071312No Hit
GCTGGCGGGGAAGCAATTTTGCAAGCTTTTGCCAGACTTGAAAAGAGAGAGA1540.10898256986561175No Hit
TGTGTCGGGAGATTTGAGGAAAATAACGTTCATAGTTGTGTTTTTGTTGTTG1540.10898256986561175No Hit
CAGCACGGGCAAGCCAACTGTGTAGCATGGACTCGAAAGCAAATATTGTACA1530.10827489084051038No Hit
TGTTGCGGGGTCTTGTATCTGTTCAGCAGGTCTGAAAGTAGTTGATGAAAAT1510.10685953279030763No Hit
GGCACTGGGAGTCTGACACTCCATTAACAAAGGGCTGCTTGTGTGCCATAAT1500.10615185376520626No Hit
AGATTCGGGGATTTTTATGGTTGGAACCAAAGTTATTCAAATAGTAAAAGGA1460.10332113766480075No Hit
TCGCCAGGTGGTTGCAGCTGAGCTTGTATAGCTTACAGAACCAAACAATATT1450.10261345863969938No Hit
ATGGTTGGGGGTACTAATTTTACTGGCCAAAAGAATGACAAATTATTCTTTT1450.10261345863969938No Hit
GAACAAGGGATGCTGAAGGCTGGAAAGTTGAGTCTGGCTTGCAACTAATATT1440.101905779614598No Hit
GTGCGGGGGGCCTCTGCAGACACAGGAGCAGAGGGGATAAGTGGTGACATGG1430.10119810058949663No Hit
ATGTGGGGGCTTGGTATGGGCGACATTGAAGGACTGATAGATAAAGTCAACG1430.10119810058949663No Hit
ATAGGTGGGTGAAAATGTTTGGTCATTCTAGTGTTTTGCTTTCTTTTCCTTA1430.10119810058949663No Hit
TAAAGTGGGATTTCAGGATGGCACCGTGTGGGAGAGAGAACTGCCAATCATT1430.10119810058949663No Hit
AAACTAGGGAAATAAGCTGGATTTTTATAGTAATAAAATTACTAGCTTCAAC1420.10049042156439526No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)