FastQCFastQC Report
Sat 31 Dec 2022
SRR5848900.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848900.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80750
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT4690.5808049535603715No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC4130.5114551083591331No Hit
ATTGTTGGGAATTAAAAAATATGCACATTCAAGAGCACAGGAAGTTTGAACT1130.13993808049535603No Hit
ATGCTTGGGGAATGGAAAGCCAACTATTTGTGCAAATAAATCACCAATGTTT1120.13869969040247676No Hit
TTGCTTGGGTGTATAATAATCATCACAATCAGGAATTCTTTTATTCAATGTA1110.13746130030959752No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG1080.13374613003095975No Hit
TTAGTGGCGGGGTGGCTCTTTCACTGGCAATTCTCAAATGATATACATTTAA1070.1325077399380805No Hit
GGACACGGGACTGGGGGTCTTGAAACATATCCTCTCAGAATTGATTATTATT1060.13126934984520122No Hit
TGTTACGGGAGGCCGTGGAGCTTGCCGTCCCACCTCCGTCCAAATCGACCTT1050.13003095975232198No Hit
TGCACGGGAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAACAAGA1030.12755417956656348No Hit
TACGAGGGGACTTGATGTCCTGCTTTTTATCTTTTTAAATACAGATTTTTTT1010.12507739938080495No Hit
GGGTCTGGGATAAATACATTAAGTTTAACTGAAATTAATAGTCAAATTTATA1000.12383900928792571No Hit
GCCTGGGACCATACCATCTCCAGAATTGTTTGGCTTGTTTTCTTTCCTAAAT1000.12383900928792571No Hit
GACGCTAGGGGGTATCAGTGTTTTTAAAAAGTGTTTGAAGTGATTGAAATGT990.12260061919504645No Hit
GCATCGGGGATATCGTTATGCTTGCCTCTTATTTGCTTGATTCTCCAGGTAT990.12260061919504645No Hit
GTTGTTGGGATGTACTGAGTTATGATAAAAAGGAAAGAATTTATTTTATCCC990.12260061919504645No Hit
CCAGTAGGGGATTAGACATCTCACTACTACTACTGACTATCACACACTGGTT970.12012383900928793No Hit
CACAAAGGGGTCTCAGGAACTGTTTGTAGCACTGGGTTGGGATGTGGACATT970.12012383900928793No Hit
AGTAATGGGAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGCTC960.11888544891640866No Hit
AGTCCGGGATTTTAAGACTTTTAAAAAACAGAATGAAGATTATTATTAAATT950.1176470588235294No Hit
AATGCTGGGCAGAGTAACACTTTAATTGTAAATAAATTATTATTTACAATTG950.1176470588235294No Hit
AAACAAGGGAGGATCATGTTTTCGCTGAGCTTCTCCCCATGTTAACTTAGAA940.11640866873065016No Hit
TGCAGAGGGATGGAAACCGAGGAAGGAGACAAGCTAAAAAAGCTGCATCCAC930.11517027863777089No Hit
GTCAAGGGGCCTGGAGGCGGCGCCGCGGAGGACGGAGCGGAAGTGCTCGCTG930.11517027863777089No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG930.11517027863777089No Hit
GTGGTGGGGCCCCCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGT920.11393188854489165No Hit
CATAATAGGGTGGCTTCAAAGAAGAAACTAAAAGCCACCTCTTGCAGGAAAC920.11393188854489165No Hit
GACAGTGGGGAGGTGGGAGGTTAGTGTGGCCCGGGCGTCCGCTCCTCAGCGG920.11393188854489165No Hit
TTCAGAGGGATCATCGGTACCTTGGAGCTGATGGCGTCGATGTAGATATTCC910.11269349845201239No Hit
TATGGCAGGGATTTGGCTGGAGGAGAAAAGAATATTAAAGTGTCATGGACAT900.11145510835913312No Hit
TGCTGTGGGACCATGTGCCTAAATGTGAGCCACCTTGGTGCACTCCCCCACA880.1089783281733746No Hit
TGCTGGGATTACAGGCGTGAGCCACCGTGCCCGGCCAACAAAGAAAGTTTCT870.10773993808049535No Hit
TTAGCAGGGGACGCTGTAACACTCTGATTTCATTGATTGAGAAAGAAAATAT870.10773993808049535No Hit
ATGTAAGCGGCGGCCCTGGCACTGCCCCTTGCATTAGGTCTTGTGTTTGATG870.10773993808049535No Hit
TGAACAGGGATTTTGGGAGCCTTTCCACTTTTTTTAAACCTTGTTCATTATG870.10773993808049535No Hit
GTCATTGGGATCATCTGAAGTATCTGTTCAATGCTTAAATGTACTGGAATGA870.10773993808049535No Hit
CTTGGACTGGGAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGA870.10773993808049535No Hit
CCTGCAGGGGCTGGTGTTCTTTTTCTGAGAAGCAAAAATATTCCCTTGTTCT860.1065015479876161No Hit
CGACGAGGGCGATCTATAGAGGGAATCCTTACTTGTGTTACACAGGAGAAAG860.1065015479876161No Hit
AGTCCTGGGATTTCTGAAATATTTGAGTTTCCAATGGTTCAATGCACAGACC860.1065015479876161No Hit
TCCTTGGGGGATGAGTGCAATATTTTAATGTTTCACTGGGAAAAATAAGTTG860.1065015479876161No Hit
GCTGCTGGGTCACAGTGTCCAGATCATTCGAGTGGAAAACATTTATAGGTGT860.1065015479876161No Hit
TGCGTAGGGGGTATTCAATAAGCTAAAGAATGTTAGAATAGCTTTTGGTCCT850.10526315789473684No Hit
TCGCCTGGGTGCCTGTAGTTCTTCAAGGAGTGGTACAATTTGGGTAGGAAAA850.10526315789473684No Hit
GACACGGGAGATTTTGTCATGTCCTGTCATCTTTTCATAGGTAAGTTTCAGA850.10526315789473684No Hit
TGCAGTTGGGATGTTGTCCTGGTGTCTTGATTTTTTGGTTCATCTGTTTTAA850.10526315789473684No Hit
AGGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA850.10526315789473684No Hit
ACTCCTGGGATAAGAAAGTGGATTTGATCCCCAGTCTCATTGCTTAGTAGAA850.10526315789473684No Hit
ACTTTAGGGATTTCACTGGTTTCCTTCTGTTTCTTGTCCATCACTTCTGTAT840.10402476780185757No Hit
GACTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC840.10402476780185757No Hit
AAGCTGGGATTAAGGCGTGAGCCACCGTGCCCGGCCAATACGTACTTTAAAT840.10402476780185757No Hit
TTTTAAGGGGATTCATACATTTGTGAATTATTTTTGGATTCTGGAATGATGC840.10402476780185757No Hit
ATGTTGGGGCTGAATGTGGCGGGCCAAACTGCAGAACTGACGAAGGAGAGAG840.10402476780185757No Hit
TGTACCGGGATTTCTGTTCTTTTACATTTGCTGAGGAGTGCTTTACTATGTG840.10402476780185757No Hit
TTTTGTGGGATTGGTGTCTATTAAATATAAGCTCTGGAACTTAATTATTTAA830.10278637770897833No Hit
TGTATAGGGTTACTAGTGACTAGTTTACTAACTAGGTCATTCAAGGAAGTCA830.10278637770897833No Hit
GGTAATGGGGCGAGGGACCAATACACATGTCCTATTTAATGAAATTAGATTG830.10278637770897833No Hit
ATAGCAGGGTATATTTACATTGTATTAAAATTATAGTTTCCAAAATTAAATT830.10278637770897833No Hit
AGTCGCGGGATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC830.10278637770897833No Hit
TCTCGGGATGCCCAACTTCAAAGGAGATGAAAATTACAATCTTACCATGTTC830.10278637770897833No Hit
TTTGTCCGGGAGGCTTTCTTTCATGCACTTTACTCATAGCACATTTCTTGTG830.10278637770897833No Hit
AACCACGGGCATCATAGAACACATACTGGAGAGAAACCATATAAATGCAGTG830.10278637770897833No Hit
TACCACTGGTCTAACTTATTGCCATGTTTATTGACTCTTGGCATATATATTT820.10154798761609907No Hit
TAATGGGGCGATCTGTTAAAGGGAAGGAGCAAACCATGTGGGCTTGTCTAGC820.10154798761609907No Hit
TCAAAAGGGGCAGTTTAGATCCATGGATTTAAATAGATCAGAACTTTAGACT820.10154798761609907No Hit
AGCAATGGGTGCGATGGGTCTTCCAGAGTTTTTATCTTACACATAGCTGTTC820.10154798761609907No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG820.10154798761609907No Hit
CGCCCGGGTTGTAATCCATGGAGCAATAGTTGGTTTAATTAAATTATGGGAC820.10154798761609907No Hit
GGACTGGGGATGCTAAAAAATTGGGTAACTGAGATGAAATCAGAATTTGTGG820.10154798761609907No Hit
TATACTGGGGGGACTGGGTGCACTGGTTCCTGAAGATGCGCGCCTATGACTA820.10154798761609907No Hit
TGGCTGGGGGCAACCACAAAAACATCAAATTATGGAGAAGTGAGTGCATAGA810.10030959752321983No Hit
TATTCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.10030959752321983No Hit
GTCACCCGGGGACTGGTAGAAATTAAAAGGATTTCAAAAACTTTCTATTCCT810.10030959752321983No Hit
GCTTTAAGTTTTTGGAATTTGAGTACAAGGAAAAAGAAGAACAAAAATAATA810.10030959752321983No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)