FastQCFastQC Report
Sat 31 Dec 2022
SRR5848899.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848899.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80348
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT4830.6011350624782198No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC4490.5588191367551153No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG1220.15183949818290435No Hit
GGACACGGGACTGGGGGTCTTGAAACATATCCTCTCAGAATTGATTATTATT1080.13441529347339073No Hit
AGCTAGGGGCATGTGACTCAAAGAAATGAGTGTCTGTGGATGGAGAGACAGA1080.13441529347339073No Hit
TATGGCAGGGATTTGGCTGGAGGAGAAAAGAATATTAAAGTGTCATGGACAT1030.12819236321999303No Hit
CTTGGACTGGGAAGTTCGAGGTGGCTACCGATTTCAGCACATGCACTACTGA1010.12570319111863396No Hit
CTTCAGGGGAGACCAGAATGTTGGGCATAGGCCATTGACAGGCCTGACAAGT1000.12445860506795439No Hit
ATTGTTGGGAATTAAAAAATATGCACATTCAAGAGCACAGGAAGTTTGAACT990.12321401901727484No Hit
AGTAATGGGAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGCTC990.12321401901727484No Hit
CACAACGGGGGAGTTTCAGCTAATGAGGTGAAGGCCACAGTGCCTCTTAAAC970.12072484691591577No Hit
CTGCAGGGTACAGTGCCCCATTATTTCATCTGGTTTCCCTTTTGCCTTTATC970.12072484691591577No Hit
TGTGATGGGATGATGTCCTGAGCTTGTTTTCCCCTATCGTGTTACTGTCACT970.12072484691591577No Hit
ATGCTTGGGGAATGGAAAGCCAACTATTTGTGCAAATAAATCACCAATGTTT960.11948026086523622No Hit
GACTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11823567481455668No Hit
GGTAATGGGGCGAGGGACCAATACACATGTCCTATTTAATGAAATTAGATTG950.11823567481455668No Hit
TGAACAGGGATTTTGGGAGCCTTTCCACTTTTTTTAAACCTTGTTCATTATG950.11823567481455668No Hit
TACGAGGGGACTTGATGTCCTGCTTTTTATCTTTTTAAATACAGATTTTTTT950.11823567481455668No Hit
CATAATAGGGTGGCTTCAAAGAAGAAACTAAAAGCCACCTCTTGCAGGAAAC950.11823567481455668No Hit
CAAAGAGGGACTTGACTCTTTCTCTTGCCTGTGCTTTTGGCCTCAAACAGGC940.11699108876387715No Hit
TGTTACGGGAGGCCGTGGAGCTTGCCGTCCCACCTCCGTCCAAATCGACCTT940.11699108876387715No Hit
AAACAGGGGAGTTCCTGATATTACCACTGAATTTCAAAAATTTCAGTGGTAA930.11574650271319759No Hit
TGCTGTGGGACCATGTGCCTAAATGTGAGCCACCTTGGTGCACTCCCCCACA930.11574650271319759No Hit
GCTTATGGGAGGGTATTCATATTGTCTTATTGCTGGTACAGTAGGACGTTTG920.11450191666251804No Hit
GCACAAAGGGAAAGACATCGCTTCTAGTAGTCACTAAAAGCTGTAGTAGATG920.11450191666251804No Hit
ATGTTGGGGCTGAATGTGGCGGGCCAAACTGCAGAACTGACGAAGGAGAGAG920.11450191666251804No Hit
TAAAGAGGGGGGAAAACTAGCTCAGATATTGGCTAGCTTATAGTTTGTTTTG920.11450191666251804No Hit
GTGGTGGGGCCCCCACTGTCAACCCAACACAGGCATGCTCATAAGGAAAGGT910.1132573306118385No Hit
TTTTAAGGGGATTCATACATTTGTGAATTATTTTTGGATTCTGGAATGATGC910.1132573306118385No Hit
AACTAGGGGGCAAAGTGAGTATGTAAATGATGGAAGAAAATGTTGGCATCTG910.1132573306118385No Hit
TCAAAAGGGGCAGTTTAGATCCATGGATTTAAATAGATCAGAACTTTAGACT900.11201274456115895No Hit
AGTCCGGGATTTTAAGACTTTTAAAAAACAGAATGAAGATTATTATTAAATT900.11201274456115895No Hit
GGATGTGGGATGCTGGATGTCATGCAGGACCACTTCAGCCGCGCGTCCAGCA900.11201274456115895No Hit
GCATCGGGGATATCGTTATGCTTGCCTCTTATTTGCTTGATTCTCCAGGTAT900.11201274456115895No Hit
ACTAGTGGGAGTATTTGATATACCTAACTTGTTATGGAGGTTGATTGATATC900.11201274456115895No Hit
TTCAGAGGGATCATCGGTACCTTGGAGCTGATGGCGTCGATGTAGATATTCC890.11076815851047941No Hit
GCAGCTGGGAGAGAATGTCATTAAATACTTGAATCTGCATGACAGTTTGACT890.11076815851047941No Hit
TTAGTGGCGGGGTGGCTCTTTCACTGGCAATTCTCAAATGATATACATTTAA890.11076815851047941No Hit
TGGTGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA890.11076815851047941No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG890.11076815851047941No Hit
GTTACAGGGGCGGTCGCCGCTGTTGTTGTGGTCCCCATGGAGCTGCCGTAGC890.11076815851047941No Hit
TGCACGGGAGTTAACAACGGACTTGGGAAATCTCAGGCTAAGAACAACAAGA890.11076815851047941No Hit
TGTCTTGGGATGTGGCATTCCTTTCTTTAAACTATAGGTAATTAAGGCAGCT880.10952357245979988No Hit
TAGCAAGGGAAGTCATGACTGATGAGGAGAGGTGATTTTTCACTGGATTTCA880.10952357245979988No Hit
ATGAAGGGGTGCAGAGTTCAGTCGAGAGTTCCAGTTGCACCAGCATCTCTTT870.10827898640912033No Hit
AAGATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.10827898640912033No Hit
ACTTCCGGGGACTCCAGACAAGATTGCAGTAATTTTGAATGCAGTTTTTCTT870.10827898640912033No Hit
TACCACTGGTCTAACTTATTGCCATGTTTATTGACTCTTGGCATATATATTT860.10703440035844079No Hit
AAACCTGGGATCTGGCATAGGGAAATGCAGCAACTTGTTTTAAAAAAATGCA860.10703440035844079No Hit
TTGCTGGTGGGGTGATTTTAGAAGCCATTATTTCTTATCCATTCTGATCTTG850.10578981430776123No Hit
AAACCGGGTGTCATAATCAAATGAGAATGTGGAATACCCAAACTCTCTGTTT850.10578981430776123No Hit
GACACGGGAGATTTTGTCATGTCCTGTCATCTTTTCATAGGTAAGTTTCAGA850.10578981430776123No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG850.10578981430776123No Hit
TCTGCAGGGATAATAATTATTTTTCTTCTATGGAACTCAGAATCCTGGCTAC850.10578981430776123No Hit
ACACTCGGGGCTTCCTTTTGCGGGTGGCGGCGAACGCGGAGAGCACGCCATG850.10578981430776123No Hit
GCGCTTGGGTGACTTCTGAGATCAGAGATTACATGTCCAGTTCAGAAGTCCA840.1045452282570817No Hit
GACAGTGGGGAGGTGGGAGGTTAGTGTGGCCCGGGCGTCCGCTCCTCAGCGG840.1045452282570817No Hit
CGCCCGGGTTGTAATCCATGGAGCAATAGTTGGTTTAATTAAATTATGGGAC840.1045452282570817No Hit
GGACTGGGGATGCTAAAAAATTGGGTAACTGAGATGAAATCAGAATTTGTGG840.1045452282570817No Hit
GGGTCTGGGATAAATACATTAAGTTTAACTGAAATTAATAGTCAAATTTATA830.10330064220640214No Hit
TAGTTGGGAATCCAATCACATTGATACCTTGTTTTATTTCATCATAATTTGA830.10330064220640214No Hit
ACGCTAGGGTAGCAGAAGGAACTCCAAGGCTGATGGTAAAGCTTCGTAAGGA830.10330064220640214No Hit
ATGTAAGCGGCGGCCCTGGCACTGCCCCTTGCATTAGGTCTTGTGTTTGATG830.10330064220640214No Hit
GACGCTAGGGGGTATCAGTGTTTTTAAAAAGTGTTTGAAGTGATTGAAATGT830.10330064220640214No Hit
TAACTAGGGGTTGTTCTATCTGCTGGATAATTAGTGTGTTAATAGTAATTTT830.10330064220640214No Hit
AAATCCGGGTATTGCTATGTTTGAATTTACATTTTCTACTTTGCTCTGTTTC830.10330064220640214No Hit
TGAGGTGGGACTGGGCTAGATGACTGGCTGGTTGCTGGGGGCAGGTGTCACA830.10330064220640214No Hit
GCACTCAGTTTATTCCCTACTCAAATCTCAGCCCCATGTTGTTCTTTGTTAT830.10330064220640214No Hit
AGCGTAGGGCTGATTCTTCTGGGAGGATTTCAGGTATCCTTTTTTTTTTTTT820.10205605615572261No Hit
CAATTTGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG820.10205605615572261No Hit
AATGGTGGGTGGAAGGCTAACCCTCTGTTGACCGTAAGCCTTTTGGTTTGGG820.10205605615572261No Hit
TCGCCTGGGTGCCTGTAGTTCTTCAAGGAGTGGTACAATTTGGGTAGGAAAA820.10205605615572261No Hit
ATAAATGGGGACAGGATAGCTTGCATTGGTGATTAATCTTGCATTGTTAAAT820.10205605615572261No Hit
CTTGTAGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG820.10205605615572261No Hit
ATACGAGGGACTGCTGTTTAGAAAATTTAAAAGTTAATGAACTTACGAAAAA810.10081147010504307No Hit
GTTCAGGGGATTTCTTGCAATTATAACCAATCACTAGTCATGAAATATACCT810.10081147010504307No Hit
ACTAAGGGATGCAGGAGATATTATTTTCTCATAGAAATTTAAAGTACGTTTA810.10081147010504307No Hit
CCAGTAGGGGATTAGACATCTCACTACTACTACTGACTATCACACACTGGTT810.10081147010504307No Hit
TACCAAGGGACTGCCAGGAGTCATGCTACCCTTTATAGTCATACCCACCTTC810.10081147010504307No Hit
ACTCCTGGGATAAGAAAGTGGATTTGATCCCCAGTCTCATTGCTTAGTAGAA810.10081147010504307No Hit

[OK]Adapter Content

Adapter graph