FastQCFastQC Report
Sat 31 Dec 2022
SRR5848898.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848898.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79717
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT4380.5494436569364125No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC3890.48797621586361756No Hit
ACGAGGGAAGGGATTCTCCTGCCTCAGCTTCCTAAGTAGCTGGGATTACAGG1250.156804696614273No Hit
ACGCTAGGGTAGCAGAAGGAACTCCAAGGCTGATGGTAAAGCTTCGTAAGGA1060.1329703827289035No Hit
ATGCTTGGGGAATGGAAAGCCAACTATTTGTGCAAATAAATCACCAATGTTT1030.12920707001016093No Hit
TCAACAGGGATAGTCGGTTAGTTGAGATAGGATGTGGTCTTAAGTAGTAAAC1020.12795263243724678No Hit
TTTTAAGGGGATTCATACATTTGTGAATTATTTTTGGATTCTGGAATGATGC1000.1254437572914184No Hit
TACGAGGGGACTTGATGTCCTGCTTTTTATCTTTTTAAATACAGATTTTTTT1000.1254437572914184No Hit
ATGTTGGGGCTGAATGTGGCGGGCCAAACTGCAGAACTGACGAAGGAGAGAG1000.1254437572914184No Hit
TATGGCAGGGATTTGGCTGGAGGAGAAAAGAATATTAAAGTGTCATGGACAT1000.1254437572914184No Hit
AAACCGGGTGTCATAATCAAATGAGAATGTGGAATACCCAAACTCTCTGTTT990.12418931971850422No Hit
GGGTCTGGGATAAATACATTAAGTTTAACTGAAATTAATAGTCAAATTTATA980.12293488214559002No Hit
TGCTGTGGGACCATGTGCCTAAATGTGAGCCACCTTGGTGCACTCCCCCACA980.12293488214559002No Hit
TCGCCTGGGTGCCTGTAGTTCTTCAAGGAGTGGTACAATTTGGGTAGGAAAA970.12168044457267584No Hit
TGTTACGGGAGGCCGTGGAGCTTGCCGTCCCACCTCCGTCCAAATCGACCTT970.12168044457267584No Hit
GGTAATGGGGCGAGGGACCAATACACATGTCCTATTTAATGAAATTAGATTG960.12042600699976166No Hit
CTTTCTGGGCTGTGTTTTCAGCAAATCATAAATTTCTGTCTACTTCTAGATA960.12042600699976166No Hit
TGAACAGGGATTTTGGGAGCCTTTCCACTTTTTTTAAACCTTGTTCATTATG940.11791713185393328No Hit
AGTCGCGGGATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACCC940.11791713185393328No Hit
TTGCTTGGGTGTATAATAATCATCACAATCAGGAATTCTTTTATTCAATGTA930.11666269428101911No Hit
ATTGTTGGGAATTAAAAAATATGCACATTCAAGAGCACAGGAAGTTTGAACT920.11540825670810492No Hit
GCATCGGGGATATCGTTATGCTTGCCTCTTATTTGCTTGATTCTCCAGGTAT920.11540825670810492No Hit
ACCTAGAGGGAGACTAATTGTTTTGTGTTAAAGCAAATTATAATTGTAGGTT920.11540825670810492No Hit
GGACTGGGGATGCTAAAAAATTGGGTAACTGAGATGAAATCAGAATTTGTGG920.11540825670810492No Hit
ATGGAAATGGGTGTCCCGTGATTTAGCAGCATTGCATGAGATTTGCGTGGCT900.11289938156227655No Hit
ACTCCTGGGATAAGAAAGTGGATTTGATCCCCAGTCTCATTGCTTAGTAGAA900.11289938156227655No Hit
TCCTTGGGGGATGAGTGCAATATTTTAATGTTTCACTGGGAAAAATAAGTTG890.11164494398936238No Hit
AACTAGGGGGCAAAGTGAGTATGTAAATGATGGAAGAAAATGTTGGCATCTG880.11039050641644818No Hit
TGGCTGGGGGCAACCACAAAAACATCAAATTATGGAGAAGTGAGTGCATAGA870.109136068843534No Hit
CTGGTAGGGATTGAAGCCAGCCATGATCAGGTATAATATGCCACTGCCATTT870.109136068843534No Hit
AGCTAGGGGCATGTGACTCAAAGAAATGAGTGTCTGTGGATGGAGAGACAGA870.109136068843534No Hit
GCTTATGGGAGGGTATTCATATTGTCTTATTGCTGGTACAGTAGGACGTTTG860.10788163127061982No Hit
ACTGTTGGGATCACAGGAGTCGGCCAACCTTTTCATAAAGGATCAGTTAGTG860.10788163127061982No Hit
TTCCATGGGAGGTCACAGCATGGAAAATGGCTTGGACAGGGGGCAAAGAATG860.10788163127061982No Hit
AGTAATGGGAATGACTGGAAAGTAAAGAGGACCAAGGTGAAAATAAAGGCTC860.10788163127061982No Hit
GCGCTTGGGGTTGAAAACTCCAGAAGAAATAAAGCTTTTCTTTGATTTCTCA850.10662719369770564No Hit
TGTGATGGGATGATGTCCTGAGCTTGTTTTCCCCTATCGTGTTACTGTCACT850.10662719369770564No Hit
TTGCTGGAGGGGTTCTATACAAATTTGCATCACTTCTTTTCTTACAGCTTTT850.10662719369770564No Hit
TAACTAGGGGTTGTTCTATCTGCTGGATAATTAGTGTGTTAATAGTAATTTT850.10662719369770564No Hit
GTTCAGGGGATTTCTTGCAATTATAACCAATCACTAGTCATGAAATATACCT840.10537275612479144No Hit
TGGGCTGGGAGAAAACAAGATGAGATTGGCATGGCTTTATTTGTTTTTTTTG840.10537275612479144No Hit
GCCTGGGACCATACCATCTCCAGAATTGTTTGGCTTGTTTTCTTTCCTAAAT840.10537275612479144No Hit
TCAGACGGGATGTCTAATATATTTCAGATTTCTGTCATAAAAGCATTTTAAA840.10537275612479144No Hit
GACACGGGAGATTTTGTCATGTCCTGTCATCTTTTCATAGGTAAGTTTCAGA840.10537275612479144No Hit
CGCGTAGGGCTTGGCTTTTTAAATTTTTTCTGTCACAGCACACCTGAAGTTC830.10411831855187727No Hit
AGGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA820.10286388097896308No Hit
ATACGAGGGACTGCTGTTTAGAAAATTTAAAAGTTAATGAACTTACGAAAAA810.10160944340604888No Hit
TTTTGTGGGATTGGTGTCTATTAAATATAAGCTCTGGAACTTAATTATTTAA810.10160944340604888No Hit
AAATCCGGGTATTGCTATGTTTGAATTTACATTTTCTACTTTGCTCTGTTTC810.10160944340604888No Hit
CGTGACGGGAGAATCTATTGATTAAAAAAGGAGCTAGTTGTCACTGAAAAAG810.10160944340604888No Hit
GACTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC810.10160944340604888No Hit
GGTGCGGGAGTGTGGCCGCGCCCCATCAGATGTTCTCCCTTCACCAGCGGGA810.10160944340604888No Hit
AGTCCTGGGCTGGATAGAGTGCAGAAGTTCTTGTTCCTCTTTTAGGAATTAA810.10160944340604888No Hit
TGGCATGGGAAAACATTTTGTTTCTAAATTAGTCTACCATTGAGTGAGAATA800.10035500583313471No Hit
CTTGTAGGGACATAAAAAGAATTTTGTGTAACTGGCAGTTTGAGCAACAATG800.10035500583313471No Hit
ACGTCCGGGCAGTTTAGGAATTGAAAATACGGTATTTTGATATATATAGGTA800.10035500583313471No Hit
GCACTCAGTTTATTCCCTACTCAAATCTCAGCCCCATGTTGTTCTTTGTTAT800.10035500583313471No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)