FastQCFastQC Report
Sat 31 Dec 2022
SRR5848895.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848895.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70656
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT4350.6156589673913043No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC3030.4288383152173913No Hit
GATCTAGGGCTTACATATAAAACTAACAAAACATGCACAGAATCTGTATGAT1350.1910665760869565No Hit
TAACTCGGGAAGGGGCCAATTCTAAGTATTCTTCACGGCAGCCAGAAGTTAA1220.17266757246376813No Hit
GATAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1150.16276041666666669No Hit
TGGTAAGGGCAAATGTCAAATATAAAACATAAAACTACAGATATTTTAGAAA1110.1570991847826087No Hit
CTTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1100.1556838768115942No Hit
TCAATTGGGAGAAGATTTATTCTGTTAATTTTTGGTCTTCATAAACCTAACT1080.15285326086956522No Hit
CGGAGCGGGCTGGCTTTAAGTCCCATGTCATTTTTTATGCACCACAATTACT1060.15002264492753623No Hit
GTCGCAGGGATGCTGACTCACTGGTACAAACAGCTGTTGAAGCTCAGAGCTG1050.14860733695652176No Hit
TTACTGGGCAGGAAGCATTTTAGCAAAAGCTCAACTGGAGGTTACTGATGGT1040.14719202898550726No Hit
TAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.14719202898550726No Hit
TTAGCAGGGGAATGAAAAAACAAAAAGTAGGAACCAAACATAAAAGGTCTAG1010.14294610507246377No Hit
TACAAGGGCGTGGAGATAATCAACACTCAGGACCTTCAGCCTTGAACGCCCA980.1387001811594203No Hit
TAGTAGGGGAAATCTATATGTGCTGGAATACTTAGAAATAAAAGTTTTGTTT970.1372848731884058No Hit
TGAAGCGGGCTTTGAACTTGTCTCCTGTCTTCAGAGTGGCAGCTTACTTCTG960.1358695652173913No Hit
TTTTCTGGGAGCGCCTGACAGAATCTCCGTGTGCTTTGGTGGCCAGCCAGTA960.1358695652173913No Hit
GCGCTGGGAGGCACAGCTGAGTCCGGAGCAGACTGAGCCACCTAAGAGGGGA960.1358695652173913No Hit
GACGTCTAGGGGGAGGCAAACTTGGGGAGTGAGAAGCCAGCCTCCCAGAGGC950.1344542572463768No Hit
GTCAAAGGGTTTACTAAGAATAAAACATCCAAATTTTAAGTATTATGAAATA940.13303894927536233No Hit
ATCAACGGGTATGTTGAGTTTTTGTTTGTCTTGGAGTATTTTCTAATTTGTC940.13303894927536233No Hit
CAGTCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.13162364130434784No Hit
TAAGACGGGGATAGTTTTACTTCTTTTGTGATTTGGACGACTTTTATTTGTT930.13162364130434784No Hit
ATAACAGGGCAAGACCAGAATTGGAGAGCTAGTCATGCCCTTTCCTACTAGA930.13162364130434784No Hit
TCATGAGGGCACTAAAGTTAGTAAAGAAAAGTTTACCATCTGAAAAAGCTGG920.13020833333333331No Hit
TAATTGGGGAAGGATGTCAAAATAGTTCATGAAAAGATACTCAACATTATTA910.12879302536231885No Hit
CTCACGATGGGACCTAAAAAGGCCTTGTTTTAAATTTCTGTGATACCTAAGA910.12879302536231885No Hit
ACTAAGGGGAGCCTAAGTATGAAATGAATACTTTACTTGAAGATCTATTTTT910.12879302536231885No Hit
ACGCAAGGGACACCAATGCGGACAAGCTCCTAGAAGCAGCAGAGCAGTTGGC900.12737771739130435No Hit
AAGAAAGGGAGATCTGAGTTTTCAGAGTGCTTCTGAAGTACAGTTTAAAAAC900.12737771739130435No Hit
CATACAGGGACCACTGAACCCTAGACATATTAAGCTGATGGCCATCTCTATA900.12737771739130435No Hit
TTAGCAGGGATAACACTCAATTGACAATAGATATGAACTGTATTTTAAATCA900.12737771739130435No Hit
GTTGTTGGGAAGCTCGAGTCAGGGAGATGGAGGAAGAAGCTGAGAAGCTAAA890.12596240942028986No Hit
ACAGCTGGGCGCCCAGGCTGGAATGCAATGGCGCTATCTCAGCTCACTGTAA890.12596240942028986No Hit
TGTTTGGGGCTGCAGCAAGTACTGACCTAGGGAGCTGCAGGGTGTCAGCCTG890.12596240942028986No Hit
CTTTACGGGAATAAATGAAAAATAGAGAGACATACCTGGCTCCAAAACAAGG880.12454710144927535No Hit
GTTACAGGGCGGACACATTTATTGAATAAGAAGCTCAAGAATATACTGATTA880.12454710144927535No Hit
TGGCTTGGGGAGTCAGATGCTGTGGTACTTGGCATTTAACAACTAGAATTCA880.12454710144927535No Hit
ACGCTGGGGATCCTCACGGAACTTTCATTCTAATGGGGGAAGACTGACTATA880.12454710144927535No Hit
CAGATAGGGCCATCAAGCAGTCGCCTTCCACCTTAGAACCTGTTCACAGTTA880.12454710144927535No Hit
GTACAGGAGAAAAAATGAATGACTAAATCATTCCTATGATGACAGAAACCAG880.12454710144927535No Hit
CGTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.12454710144927535No Hit
CTACAAGGGCAAAAAGAAATAGAAACACTTCATTAACTTTGTGATTGCCAAA870.12313179347826088No Hit
TTCACAGGGAAGTGTCTTGGATTTGTGGCTACCTCTGTTGGACTGTCCAGAC870.12313179347826088No Hit
CAGTGCGGGGATCAATGCACATCAGAGTGACATAGCCTGTGTGTCTCTAAAC870.12313179347826088No Hit
CAAATAGGGGTCTGGACACAGCTGAGAGAAACTGGTTGATGTCTGCTAATGG870.12313179347826088No Hit
ACTCAGGGTCCTGTTGTGTAACCCATGAAGAAATTGACCTTTCTTTATTGTC870.12313179347826088No Hit
GTAACCGGGGAAAAAAGTATGTAAAATTCCTTTATTTAGAAAGCAATGGATG860.12171648550724637No Hit
TTCACAGGGGAGCTTTGGCAATAGAATTGGCTAGATCAGGAAGCCTATGTCA860.12171648550724637No Hit
GAACTAGGGGACTAGGTTCTTGTCAGAATGACTGAACCTGCTTGAAGAGGCT860.12171648550724637No Hit
AAAGAAGGGATCTCAGTCTGGGGGGGAACATTCTCAAGAGGTGAAATACAGA860.12171648550724637No Hit
ATAAATGGGGATCTAGATTTTTAACTGTGATTCTATTTGTATGTTCCAAGCA850.12030117753623187No Hit
CTGTTTGGGGTCTCCAGCAAGGGATTCGGGCGAAGACAAACGGATGCACCCG850.12030117753623187No Hit
GAACTTGGGTGTGAAACCTTGCTGCAAGCACAGCCCTTATGCGGTTAATTTT850.12030117753623187No Hit
TAACTTGGGCAAAAGAAAAAGAAGAAAAAAACTAAAAAGATATTTGATATTG850.12030117753623187No Hit
CCCCCCAGGGATATGTGGTGCCTGTCATAAGCTCCAGAGAGCTGCCTTCCAC850.12030117753623187No Hit
CATGCTGGGATGGTTTCGGGGTCTAAACTTTTGTTTCGTTTTGTAGTCTTAA840.1188858695652174No Hit
ACATCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.1188858695652174No Hit
ATACACGGGATGCTCATCAATGGCGGATCAGAGAGAAAATCCTGCTTCTGGA840.1188858695652174No Hit
TCACGCTGGGATAGAGAAAGTGATGGGGCCTCCGATGAAGCAGAGGAAAGTG840.1188858695652174No Hit
AGCCAGGGGAAAGTAAAAGCTACCAAAGTGGGAATTCAAAATTATAACATGT840.1188858695652174No Hit
AAGTCTGGCTACGTGCGGGATTTAATTCATCCAGTAGTTATTCAGGAGCATG830.1174705615942029No Hit
ACAAGGGCGAGTCCGAGGGGGCCGAGCTGTCGCTGAGCCTGGCCCGCACCAA830.1174705615942029No Hit
TTTAAAGGGCTAGGCACGGGGAAGTTCTAAGAGATTTAGAAGCTCTTTGTCA830.1174705615942029No Hit
TCTATATGGGACTGTAGATTGCATTTCGGCTTTTCCTCCTTTCACATTCTTT830.1174705615942029No Hit
AACTTAGGGACTAAAAATGAGACAGAAAGGTGAATATTTGAGAGTAGCAGAG830.1174705615942029No Hit
TCACAAGGGCTCTGACTCACCTAGTCTTTTTAGTTTTATTATTTCAACAATC820.1160552536231884No Hit
CGCGATGGGGTCTTTCACCATCTGAAGCCCACTAATTTGTCTTTTATATATA820.1160552536231884No Hit
GACAGTGGGATGGCAGGTGTTGACTCTTTTAACCTCCTTTGTTGAATTTTAA820.1160552536231884No Hit
GTTCAGGGGATTTAACCCTCACCTGGTGCAAATGCATCATGTAAATTTTGAA810.11463994565217392No Hit
ATTGATGGGATATTCCTTGAAATCATTTACCAACACTGTATGGAGCATTAGG810.11463994565217392No Hit
GCCGCTGGGGAAAAGGTCCCGGAGAGCTGAGCAGTCAAGATGTGTGACTTCA810.11463994565217392No Hit
TGTCCAGGGATTTGGGGAAGGAAAAGAAAGTAGTTGTTTTACAAGGGAGGAA810.11463994565217392No Hit
TGGCCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA810.11463994565217392No Hit
GAATTAGGGCAGTGTCTGGCACGTAGTAGCTGTTCAGTAATGCTGAGTATGA810.11463994565217392No Hit
TATTATGGACCAGCACCTATTGAGCAATGTCTATTATAGTAATTTTGCATAC810.11463994565217392No Hit
ACACTTGGGCAAGATTTAACTCTTACTAAATTTATAACAGTATTGGACCATT810.11463994565217392No Hit
TATAATGGGAACAGTGCTCAAATTCAACTAGGTCTTTCAGCTTTTTATACAA810.11463994565217392No Hit
GTTGCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG800.11322463768115942No Hit
ACTAAGGGTGTTATAGGAAAACAACTTGGTATTTAAGTATGAAGTGGATGAA800.11322463768115942No Hit
CATGAGGGATAATTGTTTGACCATACTGAATCATTTACATTGTCTAATCCAT800.11322463768115942No Hit
GGGACGGGACTTCAACCAGGAACTCTACGGGAATTTCAGCGCTGCCTCCCGC800.11322463768115942No Hit
GTATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA800.11322463768115942No Hit
AGTTCTGGGGGAATCAGGCGCCGGGTAGTGGGTTGCTGGGCTGGGCTTGCTG800.11322463768115942No Hit
TTCACGTGGATGATCAATGTGAAGAGATTCCAGAAAACACTATATGTGGAAG790.11180932971014493No Hit
TGCACTGGGCGATCTCAAAGCGGGGAAAAAAGCCTTTCAAAAGTTTCTCACC790.11180932971014493No Hit
CTGCCAGGGAATATACGATTTTTTATGAATGTTTTTCATTTAATAATTATGA790.11180932971014493No Hit
TGCTGAGGGGCAAAACAAGAAAAAGAAAAGAAAGAAAAGGAAGTCTCCCAAA790.11180932971014493No Hit
TACACCCGGGCATGAAGAAAATTACATCAAGGCACATCAAAGAAGCAGCCAT790.11180932971014493No Hit
TGTTCTGGGCAAACTCAATCGGGGACTAAAATTTCCCACTGAAAATGTTAAA790.11180932971014493No Hit
TTGTGAGGGACAGTAAGGTAAGAGAAAGAGAAAGCAGGAGGGAGAGAGGAAA790.11180932971014493No Hit
AACACTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC790.11180932971014493No Hit
CAATAGGGGGCCCGCCCCTCTATGGAAAGTAAATGGTAGCTCGGAAGGGTCA780.11039402173913043No Hit
TGCCAGGGCTTCTCAATTTTGATTGCTCAGTAGGTTCACTTGGGAGAGGTCT780.11039402173913043No Hit
CAGCTCGGGCGATCTGACAGCGGGCCGAGAACCTAGCTCCCGTGAAGAACCA780.11039402173913043No Hit
TTGATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC780.11039402173913043No Hit
ACTTCGGGATGACTGAAATTGTTATGGACTCTTTCAGAAGCTTAGTTATTAA780.11039402173913043No Hit
TTTGGAGGGGTCAGGTGAAGCAGAACAATGACTCATTTATTTCAGCCTGGTT770.10897871376811595No Hit
CTAACTGGGACTTGTGCCACTTAACATGGACTCTTTCTTCTTTGCCCTCACT770.10897871376811595No Hit
TCGCCAGGGACTACATAGCATATACCCAAAATCACTGAAGAAAATCGCTGGC770.10897871376811595No Hit
GAAATAGGGTTCCGATCTTATGAGGGGACATCAAAAAGCTTTTAGAAAATGG770.10897871376811595No Hit
TTTTTTGGGCCACCACAAGGATGACCCTCCTCCTCCTGAAGATGATGAGAAC770.10897871376811595No Hit
AGATAGGGGCGAGGATGAGTGCAGCACGCAGGAGGCCGACGGCCAGAAGATC770.10897871376811595No Hit
AGTTGGCGGGAAAGACGGATAATTTGCCTAGTGGGATTGGGCCTGGTGGTCT770.10897871376811595No Hit
GTAGAGAGGGAGCAGTCTTCTCAGTAGCCCAGAGTTTCCAGGAAACAGGAAT770.10897871376811595No Hit
GCGACAGGGAGATGAAAATACCATAGATTATCATAATGAGGGCACTTGCCCA760.10756340579710146No Hit
AACATAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC760.10756340579710146No Hit
CATATAGGGGTTTCAGATCCAGAATCAAATCCTCACATTTAGATTTGACCTT760.10756340579710146No Hit
TTCAAAGTGGGAGTTCGAGGAAGTAGTTTTTAAATGTGAGCTTATAGATAGA760.10756340579710146No Hit
CTATCTGGGGTGGATGGTAGGGCAAATATTATTATGGCCATTTGGTGAATGA760.10756340579710146No Hit
ACTACGGGGACCAAGCAAGACTCCGTCTCAAAAAATTAAAGTTCGTGATACT760.10756340579710146No Hit
GAAGCCTGGGAAAATTAGCACTGTAAAATAATATGTAAAGGACTTGGAATGT760.10756340579710146No Hit
AGTATAGGGCCAGGATGATGATCCTTGTCCAGCTTGTTGCTGATCTTTGCAG750.10614809782608695No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT750.10614809782608695No Hit
GATTAGGGCCAGATAACCTGTAAGGCGATTCTGTATGTTCTTTCAAAACTCT750.10614809782608695No Hit
ATGGTAGGGGAATTACTATGTAATTCTAATGAATTAATTTTAAGTGAATTTT750.10614809782608695No Hit
TAGCAAGGGGATCTAAGTCTGGGACTTATACACAGGTAAAATTAGTTTTTTT750.10614809782608695No Hit
GGACTAGGGTGCCAAATCACCTGTCAAAGTAGTTGCAGCTTTTTGTATTTCT750.10614809782608695No Hit
AATATCGGGAACAGCAGCAGCAACAGATACAACAGTTGCGATTCTTGCAGCA750.10614809782608695No Hit
TAAAACGGGACAGTGGCATGTGGAGACAACAGTGTGTCCCAATGACTTTTCT740.10473278985507248No Hit
GTCATAGGGATTTAAAAACTGAAGAATGTTCTCTTCTTGCTCAGTTCCCTAA740.10473278985507248No Hit
TGTTCAGGGATATATTTACAGTAGGAATAGACGTAGACACACGAGCATATTT740.10473278985507248No Hit
TTAGCAGGGAAGACTAAAATCTAGTAACAGGTGTACCATAGTTCTTGAAACA740.10473278985507248No Hit
TTCACAGGGACGTGGCAGATTAAAAACAGCAAAGTAGAAAGGAGCAGCAGAC740.10473278985507248No Hit
TCACCAGGGATCTGCTCCCATACCAAGCTGGTAGCCAACGAGTCTGTACCCT740.10473278985507248No Hit
TGTTTTGGGAGGCGATCACAACGTGCATTGTGCTTCTTTCTTTGGGAATATT730.10331748188405797No Hit
GCCTATGGGCGATCTTCGGCGGGGTCTTCCAAACTCCTGTTAATGTGAATAT730.10331748188405797No Hit
CTCAAAAGGGCAAATCTGAGTTATTGGCCTTGAAGAGGAGATATAGAGAGAG730.10331748188405797No Hit
ATAACTGGGAAAGGGAGAACACCAACTATTGAATAACCCTCAACCACTTCCT730.10331748188405797No Hit
GTGTACGGGCTGGTTTCCTTCCTGAGTCTGGCACAGACGGGGCTATTGTAGT730.10331748188405797No Hit
TTATATGGGTAACTGAAGCAATTTCTAAGTAAAGTGCTGAAATGGTGGCTTG730.10331748188405797No Hit
CATGGGGCACACGGTACTGTAGCCCCCAGTTCCTCCCTAGCCTTCCATCCCT730.10331748188405797No Hit
TATCATGGGAAGAGCAGCTTTCATTTGTTTTGATGAATAACCATATTTTCCT730.10331748188405797No Hit
GATAAAGGGATGGGAACGACAGCTAAGTGTGGTGAAAAACGCGGGGATCCAA730.10331748188405797No Hit
CATGCTGGGCTTGGGCCTCAGGAAGAGGTTGAACAAAAACAAATTCTTTTAA730.10331748188405797No Hit
CTCAACGGGTTCATTTTCTCTTAGGATAGATACAGTTTTGTATTTCCTAGCA730.10331748188405797No Hit
GGTTCGGGGAACTCTGACCAATGGAGGATTATAATTTTTTTATTAGCTCTTT720.10190217391304347No Hit
AAAAGCCGGGCTGCTTCTACATCTTGAGTTTTTTAATTTACTTTTTTTACAC720.10190217391304347No Hit
TTTGCGCGGGAGCCCAGAGACAGATGGCAGAGCTATCTCTCTCATAGCTTTT720.10190217391304347No Hit
TGATCAGGGATAGTTTGAAAGAACCAATAGTGTATTACAGGGAGTTTAATTA720.10190217391304347No Hit
TTGTATGGGAATATGGTGAGATCAGACTCCCTAAGACTCTTTTCAGGTTCAT720.10190217391304347No Hit
AAACCGGGATGGTACCACTGAAACCCTGACCCAGAAAAGTGGCTTGCTTGGA720.10190217391304347No Hit
CATGGGGAGAATAATCATACTTCTTTTTGAAATGGAACTATGCAAAGTCTTG720.10190217391304347No Hit
CATGGTGGGATTGTGATAAATCTTTGCAAGTTTTGTCAGTTTTTGATTCTTG720.10190217391304347No Hit
AGTTTTGGGCACTCTGTTTACTGACTGTGTTTCCTTTCATGGACCACTTTTC710.10048686594202898No Hit
AATCATGGGGCACAAAAAATGAATAATAATGTAACAGTTTCTGGGAATATGT710.10048686594202898No Hit
TAGCCTGGGCGACAGAGGGAGACTCTGTCTCAAAAAAAGAAAGAAAAAAAGA710.10048686594202898No Hit
ACTGCGGGATTGGCTGTGGGTTTGTCATAAATAGCTCTTATTATTTTGAGAT710.10048686594202898No Hit
TTGCTAGGGTAGCTTTTAAAATTTAAAGTGTTTTTGAGTGTGAAAAGTTGAG710.10048686594202898No Hit
AAGCGGGGCCAAAAAGGAAACATGAAAACACTCTCATATTCTTTCTGTTTTC710.10048686594202898No Hit
ATTAAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA710.10048686594202898No Hit
ACCACTGGGCTCCACTTTATAAGAATGCCAAATCAAACTGTACCATTTTACT710.10048686594202898No Hit
ACCGTAGGGCGTAAGGAAAGTATGGAAATTCACATTTGGAAGTGTGAACAAT710.10048686594202898No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)