FastQCFastQC Report
Sat 31 Dec 2022
SRR5848894.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848894.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23697
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC3921.6542178334810314No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT3711.565599020973119No Hit
CTTCAGGGGAGAAAATGCTCTATGTCACCAATTATTTGGGAGGTGCGGGTCG1950.8228889732877579No Hit
ATGCCTGGGATGACATGATCTTATACCTAGAAAAGCCTAAAGGCTCCTCCAA1830.7722496518546651No Hit
TCCACAGGGCAACTTCAGAATTCGAGGAAGATGATGTGAATCTTCCAATTTT1700.7173903869688146No Hit
CTTACTGGGACCCTGATCGCATCATGAATACCTTCAGTGTGGTGCCTTCACC1610.6794108958939951No Hit
AAGCTCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1600.6751909524412373No Hit
ATCCTCGGGCTGCCAAAATTATGGATCCGGATGAGCAATTGGAAACCCTTCA1580.6667510655357218No Hit
GGTTCTGGGTCACTGTCCATCTGCGTTCCCAGAGAACAATTGTGAAGAGTCC1550.6540912351774486No Hit
AGTCAGGGGTCAGACTAACAGTGGACCTCTCTGCAGAAGCCCTACAAGCCAG1550.6540912351774486No Hit
GTGCTAGGGCTCAAACAATGGATGACACAAAACTATGAGAGTGACAAAATGG1540.6498712917246909No Hit
TAGGTGGGGGGCCGCAGAAGGGGTCCTGGTGACGAGTCCCGCGTTCTCTCCT1520.6414314048191755No Hit
TAAGGTGGGACGACACGTTACTAGCAGTCACTTGATCTCCGTGGCTTTGGTT1490.6287715744609023No Hit
GGTTCCGGGCTGGCAACTGGTGCCAATGTTATTCTAACCACTGGTGGAATTG1490.6287715744609023No Hit
TGATAGGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG1470.6203316875553868No Hit
AGCCCTGGGAAGAAGGGATCTATCACCTCTGTACAGGCTATCTATGTGCCTG1410.5950120268388404No Hit
ATTTTTGGGTATGAGAAGGCAAATGATGTGAGGATTAGTATCTTGAGATTCA1410.5950120268388404No Hit
TCGTACGGGTCGCGCTGGGACCATAAACACATCACCTTGATTTTTAATCCCA1410.5950120268388404No Hit
CCAACCGGGATATGTAAATAGCTTCCCACTTCTGGACCCATCTGATGCACCT1390.5865721399333249No Hit
CTAGAGGGGCTGAGTCCTTGACGCTGGCTCTGTCCCAGGATCTCCACAGACC1370.5781322530278095No Hit
ATTGTAGGGTCTGATTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAA1360.5739123095750517No Hit
GCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGTGGCCAAGTT1310.5528125923112631No Hit
TTGCAGGGGAGACCAGCTGTATTTCTTTATAAACAACAACATCAGAGCATAT1280.5401527619529899No Hit
TGCACTGGGGCGAAAAAGGCGCTGGAGCGTGCGCGCCAGGTGCAGCTCGCCG1250.5274929315947167No Hit
TGATGGGGTATAAAGAAAAACAAAAGTCTTTTTTTCTCAAGGATGTAAGAAT1230.5190530446892012No Hit
TTTTGCGGGGAGCAGCGAGCCTGCGTCTGTCTCAGCTGTCCAGCGCTGAGGG1220.5148331012364434No Hit
ATTCAAGGGACAGACAACATCTACTGAGCTAGTTGATTGGTGATGCTTCTAC1220.5148331012364434No Hit
GTTACAGGGTGTATGGGTTTATTTCTGGACTCTCAATTCTATTCATTTGATC1190.5021732708781703No Hit
GTGAAGGGGTAAAGCACCAAGCAGGCTTTCAGGGTCCCTGATTTCAGGCCTT1190.5021732708781703No Hit
GATTCAGGGCAGATCCTGGAGATTGTCCTGGCCTTTGGCAACTACATGAACA1170.49373338397265476No Hit
TCTCAGGGGTGTGATATATCTGTGCAATGGAATATTATTTGACAATAAAAAA1170.49373338397265476No Hit
GCGTATGGGACCCTTTTGCCTTTATTGTCGACGTGACTCACCAGACGTCGGG1150.48529349706713926No Hit
CCCATAGGGACTCTCTTTGATCTCTATACAAGAAAGCTGGAAATTAAAAACA1150.48529349706713926No Hit
GTTCACGGGAAAATCAGAAAGTATGAGATCCTGTTATGTTAAGGGAAATCCA1090.4599738363505929No Hit
TAACACGGGAGATGAAAATCTCTTGTTTTGAACGAAGATTTTAAAGAACTCA1040.43887411908680424No Hit
TCTCATGGGTGCCTTCCCTAATAAGGACATGAAACTGGAGAACCACAAACCA1030.4346541756340465No Hit
AATATTGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGTCT1000.4219943452757733No Hit
AAACCAGGGCTAACTTCTCCAAGGGCACTGGCAAAGGAGATGTACAGAAGAC1000.4219943452757733No Hit
GTCGCCGGGCACCCAGAGGCCTGCTCTCCTCACACATTGTGTGGTTTGGGGT970.4093345149175001No Hit
CCATCAGGGTGTGGAGAATCATCAAGATATGAATTGAATCGCTGCGATACTG970.4093345149175001No Hit
AACACCGGGTACTTTGGATATGGATTTAAATGGTCTCTAAGAGCCGGGGGTA930.39245474110646916No Hit
ACCAATGGGTCCATTAGTAGACTTTCTGGGTAATTTGTCTTTTTAAAAATTA920.38823479765371144No Hit
AGACTGGGGGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTT920.38823479765371144No Hit
AGCCACGGGCAAAGGGCGGTACCTGCAGATGAAGGCGAAGCGACAGGGCCAC900.379794910748196No Hit
CGTAATGGGTGACCAGTACACAATTCATTCTACCTGTGAGAGTATAAGAGTT900.379794910748196No Hit
ATGAAAGGGTAACTGAGTAAAGCAGAAACATGTTAAGTAGAGACATGAAAGA890.37557496729543827No Hit
GGTGATGGGCAGAGAAAGTGGTATACTGAAAGTTTTGATTAGATTTTATAAG890.37557496729543827No Hit
TGGCTGGGTGAACCAGAGGCAAGAACACCAATTGGAAGGCTGATGCTGTCAT890.37557496729543827No Hit
AATGCAGGGACACTCGTCCCCAGAGGCACATTCGTGCACATTCTCACAGACA880.3713550238426805No Hit
ATCATCGGGGAGTATTTCTTCTGAAGTAGATGTAATCACTTCCTCTCTAGCA870.36713508038992276No Hit
GGGACTGGGTTTAAGTGATATGGCAGATGATTACATTTCTTACAATATTGAT850.3586951934844073No Hit
TTAATGGGGTGACTTGAGTCCAGTGAAATCTCATTAGGGTTTAAGAATATTT850.3586951934844073No Hit
GTGAAGGGTAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGAACTG850.3586951934844073No Hit
AATAGCAGGGGAAAGAAAAATTAAGAGGCATGCTAAAAATAATTGAGGTTTT840.3544752500316496No Hit
AGGAAAGGGTGGAAAATGAAAATAGGGAAAATAATTGAATCATTTTAGAAGT840.3544752500316496No Hit
GAACAAGGGAAGGACTTAAGTGTCTCCTCCAACAGTACGAGATAATTATGTA840.3544752500316496No Hit
GAAGAAGGGCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAA840.3544752500316496No Hit
AACAAGGGGTGTGCAGCCAAAGTTGAGAGCTAGGGAACAGATGGGGGTGGGG830.35025530657889187No Hit
TAAACGGGGTCAAGGGGTCACTTGTGAAATTGTAAGTTTCATGGGTAGCGAA830.35025530657889187No Hit
ATTATGGGGATACTGTAGAAACCAGCTCATGTGTACCTCATATCCCATCCTT810.34181541967337636No Hit
TAGGCCGGGATCTGACATGGTGCATCTGGAGACAACATCTGATGGGATCCAA800.33759547622061864No Hit
GTCCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA770.32493564586234547No Hit
CTCGCTGGGGCAAATTGTGTATGGCAGCGAGTTACAAACCACAGAAAATGGG700.29539604169304134No Hit
GGACAGGGGTTTACTACATATTGAAGGGTGTATTCACTTGGAGAAGTAGGAG700.29539604169304134No Hit
GATTCCGGGGGCTTGGTCACTTCGTGGCTAAGGTAAGAACGTGCTTGTGGAA680.28695615478752584No Hit
TTTTGCGGGCCATTTTGGCTTGATCTTAATCTTTTTTTCCCCCCTTTTCTCT680.28695615478752584No Hit
ACGAATGGGATTTTCGAACAAGAACATGTGATTTTTAAATCCATATATTGAA670.2827362113347681No Hit
TAAGATGGGAATATATAAGCATTAACAACAACAACAAAAAATTTAAAACAAG660.2785162678820104No Hit
TAGACAGGGGTCAGAGCATTCTGTTATTCTTAACAAGTGTTCTGAAGAGCAA650.27429632442925267No Hit
GTGATCAATCTATTAAGAAATGTTTGCTCAGAAGCTGCTCAGAAACGTTCTC650.27429632442925267No Hit
CTACTGGGGGATTCCCTGTTTAATAAATGGTGCTAGGAAAACTGGCTAGCCA610.2574165506182217No Hit
GCCGGGGTGTGAAAGCAGAGAAATAACTTGTAAATGGATAATTTTGTTCTCT610.2574165506182217No Hit
GATCATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA600.253196607165464No Hit
GAAATGGGTATCAGCACAAACAAAACTTAAATCAGGGTACATGACATGAAGT590.24897666371270624No Hit
AATCGAGGGCCAAACCAGACAGACAGACAAAAAATTTTTTATTGCTAATGGT580.24475672025994852No Hit
ACGAAGGGTGTGAATTTTAGATTTGGATGATCTCAACAAGTTTTCCCTTTTG580.24475672025994852No Hit
GTTAATGGGTATACAAATATATACACACATACACCAATGCCCAAGTTCCACT570.2405367768071908No Hit
TTTGCAGGGAGCGAGAGGCAGCAAGGTGGCCACAGTGTCACTGGTCAGGTGC570.2405367768071908No Hit
GAGGTGGGAGAATTGCTTGAGCCCAGGAGGTCGAGGCTGCAGTGAGCCATGA550.23209688990167532No Hit
TTTAGAGGGTTATGAGTGGAGAGACATGTACATGTTAAAAGCATGTTGCATT550.23209688990167532No Hit
TGTCATGGGGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCT530.22365700299615984No Hit
AGAATAGGGTTGATGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATG520.21943705954340212No Hit
ACAAATGGGGGAGCTCAGCAAAGAGGCTACAGCTTTTTTGTTTCCACTTAGG520.21943705954340212No Hit
GCGATGGGGTCTGTTATATTTGGACAATGAGTATTCCATGATTATTTATTGC520.21943705954340212No Hit
CCATGAGGGTCTCCTGCCGGGATTACAACACTGTGGTGTTGTAAATTGTGGT520.21943705954340212No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT510.2152171160906444No Hit
TGAATGGGGGTTGTCTGGTATCCTACTAGTGACAGGTCCTGTTTATCAGTTT510.2152171160906444No Hit
ATAGCAGGGATGTACACCATAGGGCTCAGTTTCCCTGAGGAATTAAAGTCAT510.2152171160906444No Hit
ACTAATGAGCCTTATTCCATTTCCTGATGTTTTAAAAGAAGAAACACTGCCT500.21099717263788664No Hit
GTCCAAGGGTAACCGGAAGGAAGACAAGGAGGAGGACAAATGTTAAAGAAGT500.21099717263788664No Hit
AGGTTAGGGGCTCAATACAGCAAGGACTTTGTTAGCTGTTTTTGTTCATATA500.21099717263788664No Hit
ATACTAGGGTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCT500.21099717263788664No Hit
AAACCAAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT490.20677722918512892No Hit
AGCTAGGGGTTTAGAGAGGGTTTGTTAAGGTTTATACTATTTTTGGATTGTG490.20677722918512892No Hit
AATAATGGGGTATTCATAAATGTGTTTCACGTTTCTGAACCTGACATACTGT490.20677722918512892No Hit
GGACCAGGGGCTTCAGGGATTCCATAAACATTTAAACAATGTTCTAAGGTAC480.2025572857323712No Hit
TAGAAGGGTAAGGAATGAATTTGCATTTAAAATCTTAATGAATGTTTTCAAA480.2025572857323712No Hit
TAGGCTAGGGGCCGGCACTGGGAGCAGCCGCCAGCAGGGAAATCTGGCCTGA480.2025572857323712No Hit
TACACTGGGCTTTAATTAAAATGATTGATAATAACCACTTTATTAAAAACCT480.2025572857323712No Hit
TTAACTGGGCATGGTTCTCAATTTATTTGGGGGGAAATACCTTGAGCAGAAT460.19411739882685572No Hit
ACCGTAGGGGTCCAGCAGATACTAGGTGCCTGGAATCATGATTGGCATTAGG460.19411739882685572No Hit
ATTCGAGGGGTCAAAGATGGGAAGAAAGATGAAAAACAAGAAATAGTTGTGT450.189897455374098No Hit
AATTAGGGCCGCGCCGCCTCCGCCGTGCCGCCGACCACGATGTGGGCTCTGA440.18567751192134024No Hit
GGCTATGGGTGCAGTGACTGCAGAATACACATTCTTCTCCTCAGCATATGGG440.18567751192134024No Hit
GCTAAGGGGCAGGAAAAACATTGAAAGTTTGACACCTTAGATCAAATGGACC440.18567751192134024No Hit
AATGCGGGGTGATGACCAGGCTGGGCACAGTGGCTCACGCCTATGATCCCAC430.18145756846858252No Hit
AGGTACGGGGTGAGCCTGGCTGGGTCTGCAGATGGAAGGCCTTGTTCAAGTA430.18145756846858252No Hit
CCGAACGGGTGGATTCTAGATTAGATGACAGTGTTGTATCAATGATAAGTTA420.1772376250158248No Hit
TCTAGCGGGTTTCCTTCTGTTTCCACTTCTGATGAGTTGTGGCAGCATTAAC420.1772376250158248No Hit
CAGGCCGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTA400.16879773811030932No Hit
ATGATAGGGGTTATTAAGCATTTGATTTTCTGTGTCCTTAAGTACTTCCTGA400.16879773811030932No Hit
TGTTGTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT400.16879773811030932No Hit
ATAGGAAGGGTTTGATTCTTGTTTTTCAGTGGTCTTGCCAGAACAAAGTTGC390.1645777946575516No Hit
CGTATGGGGAAGATCTCTTGGTAACAATTGACATTGCACTAGAATTTGAATG390.1645777946575516No Hit
TCCCTGGGAAGTGCCAGGTTCAGGATTTAAAAGTTTTTGCTCTTACCTACTC380.16035785120479387No Hit
ATGCTTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT380.16035785120479387No Hit
GGGCCGAGGATACCAAACATATTTATGAGATCTATTGTTACTTAAAAATACT380.16035785120479387No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTA380.16035785120479387No Hit
TAGACTGGGTGGCACCATTGACTTCCCCGAATTTTTGACTATGATGGCTAGA370.15613790775203612No Hit
ATAATAGGGTACTGAATGGTAGAGATGGTTCTATACAGGATGCTTTAGGAAA370.15613790775203612No Hit
ATTTATGGGATCCAGACATGTGTCAGATTAGCAGTGGGCCAGAATTAAATTC370.15613790775203612No Hit
CACGCCCGGGGATGCAAAAGCAAACTTCAGTCAGACCTGTATCCAATAAGTC360.1519179642992784No Hit
CGTCGTGGGCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAA350.14769802084652067No Hit
ACCGGGGTCTGCGTCTGGGCTCACGCCTGTTATCCCAGTACTTTGTGAGGCC350.14769802084652067No Hit
GCTGACGGGCGAACAGCAAAGCATTCTGTAGAAAGAGGTGTGTAGGTGCTGG350.14769802084652067No Hit
ACTAGTGGGTATCTGCTTTGCTTATTTGTTGCATGGGCAGCTGGTATTTAAT340.14347807739376292No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCTTATAC340.14347807739376292No Hit
TAACTAGGGTTAGCAGGCAACTGACTTCTCTGAGCCTCTGCCTGCTTTGTTG330.1392581339410052No Hit
ATTGTGGGGACCCATTAAAAAGGAAGCAGAAATACTGAGACATGAAAGGCAT330.1392581339410052No Hit
GAATAGGGTCCCAGCTATTCACGAGGCTGAGGCAGGGAATTGCTTGAACCCT330.1392581339410052No Hit
AAAACAGGGTCTTGATGTTTAGAAAAGTATTCGTTACATCTTGTAGGATCTA320.13503819048824747No Hit
TTTCTAGGGTTTGAAAATCAATCTCATGGAAGTTTCTATTTGCTTTTATTGC310.13081824703548972No Hit
CTATGGGGGATCATGATGGAATACCAAAAAGATATCCTGTGATAAAGAAATA300.126598303582732No Hit
TACGCTGGGGTTTACCAATCAACAGCATGTCTACTGCTTGGATCCATACAAA300.126598303582732No Hit
GGTCTGGGGTTACTGCTTCCTCAAATCTTTCGTAAGCAACTTCCTCTCCTTG280.11815841667721652No Hit
CACACTGGGATGGTTACTTGCTCGTGCGTTGCCACACTGTGTTATAGTTTGC280.11815841667721652No Hit
ACCACAGGCTGGCATTTGAAAACTCCTCTTTGCCATACCCAACCTATCTCAA280.11815841667721652No Hit
AATTGGGGGTATAACAGAGATGTTTAGATGCTGTCACTTCTACTAACATGGT280.11815841667721652No Hit
GTAAGTGGGGAAGCTGTGATGGGAAGGAGGCCTGCACTTGGACCTGACCTCA280.11815841667721652No Hit
TAATTTTGGGTACAGCCTTGAATGTGAATAATTATTGTAAACTATATTTTAC270.11393847322445878No Hit
TAATATGGGGTCCCAGCTACTCGGGAGGCCGAGGCTGGAGGATTCCTTAAAC270.11393847322445878No Hit
GTCTGCGGGTGAGGACTTCCTTGGCCTTAGATGAGTCCCTATTTAGAGGAAG260.10971852977170106No Hit
TTAATGGGGTTTGTGTCTTAGTTTTATATGCATCCTTATTTTTAATACACCT260.10971852977170106No Hit
ATTGCGGGGTCCGATCTACTGCAGGGATGTGTTAGCTGTGAGAATGACTTCT260.10971852977170106No Hit
GACCTGGGGCTGGGTGACAGAGCGAGACTCCGTCTCAAATAAAAAACAACAA250.10549858631894332No Hit
GTGACTGGGCTCCAATGGTTTAATGGGATAGAGGAATCAATGTTTATATTTT250.10549858631894332No Hit
TGTGGTGGGATGCTGTTGTTTCTAATTATACCTATTTTTCAAGGCTTCTGTT250.10549858631894332No Hit
GTACAAGGGTGGTGTGTGATGTTCCCAAATTTTTTTTTTCTCATCAACATTA250.10549858631894332No Hit
GATAATGGGTAGTCAATATATTTTTTTTTTGGCGAGTCACCAGTGACCCGAG240.1012786428661856No Hit
CTGAGAGGGGGGCTCACTGCAACCTCCACCTCTCAGGTTCAAGCGATTCTCC240.1012786428661856No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)