FastQCFastQC Report
Sat 31 Dec 2022
SRR5848893.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848893.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23563
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC4091.735772185205619No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT3641.5447948054152698No Hit
ATGCCTGGGATGACATGATCTTATACCTAGAAAAGCCTAAAGGCTCCTCCAA1880.797861053346348No Hit
TCCACAGGGCAACTTCAGAATTCGAGGAAGATGATGTGAATCTTCCAATTTT1740.7384458685226839No Hit
TGATAGGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG1740.7384458685226839No Hit
CCAACCGGGATATGTAAATAGCTTCCCACTTCTGGACCCATCTGATGCACCT1690.7172261596570895No Hit
CTTACTGGGACCCTGATCGCATCATGAATACCTTCAGTGTGGTGCCTTCACC1690.7172261596570895No Hit
GGTTCCGGGCTGGCAACTGGTGCCAATGTTATTCTAACCACTGGTGGAATTG1670.7087382761108518No Hit
AGTCAGGGGTCAGACTAACAGTGGACCTCTCTGCAGAAGCCCTACAAGCCAG1650.700250392564614No Hit
GTGAAGGGGTAAAGCACCAAGCAGGCTTTCAGGGTCCCTGATTTCAGGCCTT1640.6960064507914951No Hit
ATTTTTGGGTATGAGAAGGCAAATGATGTGAGGATTAGTATCTTGAGATTCA1620.6875185672452574No Hit
CTTCAGGGGAGAAAATGCTCTATGTCACCAATTATTTGGGAGGTGCGGGTCG1590.6747867419259008No Hit
AAGCTCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1570.6662988583796631No Hit
ATTGTAGGGTCTGATTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAA1550.6578109748334252No Hit
GTCGCCGGGCACCCAGAGGCCTGCTCTCCTCACACATTGTGTGGTTTGGGGT1430.6068836735559988No Hit
TTGCAGGGGAGACCAGCTGTATTTCTTTATAAACAACAACATCAGAGCATAT1400.5941518482366421No Hit
GGTTCTGGGTCACTGTCCATCTGCGTTCCCAGAGAACAATTGTGAAGAGTCC1380.5856639646904045No Hit
CTAGAGGGGCTGAGTCCTTGACGCTGGCTCTGTCCCAGGATCTCCACAGACC1370.5814200229172856No Hit
TAGGTGGGGGGCCGCAGAAGGGGTCCTGGTGACGAGTCCCGCGTTCTCTCCT1350.5729321393710478No Hit
GCGTATGGGACCCTTTTGCCTTTATTGTCGACGTGACTCACCAGACGTCGGG1290.5474684887323346No Hit
TCGTACGGGTCGCGCTGGGACCATAAACACATCACCTTGATTTTTAATCCCA1270.5389806051860968No Hit
ATCCTCGGGCTGCCAAAATTATGGATCCGGATGAGCAATTGGAAACCCTTCA1260.534736663412978No Hit
GCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGTGGCCAAGTT1250.530492721639859No Hit
GATTCAGGGCAGATCCTGGAGATTGTCCTGGCCTTTGGCAACTACATGAACA1240.5262487798667402No Hit
TCTCAGGGGTGTGATATATCTGTGCAATGGAATATTATTTGACAATAAAAAA1240.5262487798667402No Hit
GTGCTAGGGCTCAAACAATGGATGACACAAAACTATGAGAGTGACAAAATGG1230.5220048380936214No Hit
TAAGGTGGGACGACACGTTACTAGCAGTCACTTGATCTCCGTGGCTTTGGTT1190.5050290710011458No Hit
GTTACAGGGTGTATGGGTTTATTTCTGGACTCTCAATTCTATTCATTTGATC1180.500785129228027No Hit
TTTTGCGGGGAGCAGCGAGCCTGCGTCTGTCTCAGCTGTCCAGCGCTGAGGG1110.4710775368161949No Hit
AATATTGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGTCT1100.46683359504307603No Hit
AGCCACGGGCAAAGGGCGGTACCTGCAGATGAAGGCGAAGCGACAGGGCCAC1060.4498578279506005No Hit
AACACCGGGTACTTTGGATATGGATTTAAATGGTCTCTAAGAGCCGGGGGTA1060.4498578279506005No Hit
ATTCAAGGGACAGACAACATCTACTGAGCTAGTTGATTGGTGATGCTTCTAC1040.4413699444043627No Hit
CGTAATGGGTGACCAGTACACAATTCATTCTACCTGTGAGAGTATAAGAGTT1040.4413699444043627No Hit
GAACAAGGGAAGGACTTAAGTGTCTCCTCCAACAGTACGAGATAATTATGTA1030.4371260026312439No Hit
TGATGGGGTATAAAGAAAAACAAAAGTCTTTTTTTCTCAAGGATGTAAGAAT1000.4243941773118873No Hit
GGTGATGGGCAGAGAAAGTGGTATACTGAAAGTTTTGATTAGATTTTATAAG990.4201502355387684No Hit
AAACCAGGGCTAACTTCTCCAAGGGCACTGGCAAAGGAGATGTACAGAAGAC970.41166235199253065No Hit
ATGAAAGGGTAACTGAGTAAAGCAGAAACATGTTAAGTAGAGACATGAAAGA950.40317446844629296No Hit
AGGAAAGGGTGGAAAATGAAAATAGGGAAAATAATTGAATCATTTTAGAAGT950.40317446844629296No Hit
CCCATAGGGACTCTCTTTGATCTCTATACAAGAAAGCTGGAAATTAAAAACA950.40317446844629296No Hit
GTTCACGGGAAAATCAGAAAGTATGAGATCCTGTTATGTTAAGGGAAATCCA950.40317446844629296No Hit
ATCATCGGGGAGTATTTCTTCTGAAGTAGATGTAATCACTTCCTCTCTAGCA930.3946865849000552No Hit
AATGCAGGGACACTCGTCCCCAGAGGCACATTCGTGCACATTCTCACAGACA920.39044264312693633No Hit
TCTCATGGGTGCCTTCCCTAATAAGGACATGAAACTGGAGAACCACAAACCA920.39044264312693633No Hit
ACCAATGGGTCCATTAGTAGACTTTCTGGGTAATTTGTCTTTTTAAAAATTA910.38619870135381745No Hit
TGCACTGGGGCGAAAAAGGCGCTGGAGCGTGCGCGCCAGGTGCAGCTCGCCG910.38619870135381745No Hit
AGACTGGGGGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTT900.3819547595806986No Hit
AACAAGGGGTGTGCAGCCAAAGTTGAGAGCTAGGGAACAGATGGGGGTGGGG890.37771081780757965No Hit
AGCCCTGGGAAGAAGGGATCTATCACCTCTGTACAGGCTATCTATGTGCCTG890.37771081780757965No Hit
CCATCAGGGTGTGGAGAATCATCAAGATATGAATTGAATCGCTGCGATACTG870.36922293426134195No Hit
TGGCTGGGTGAACCAGAGGCAAGAACACCAATTGGAAGGCTGATGCTGTCAT860.3649789924882231No Hit
GTGAAGGGTAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGAACTG860.3649789924882231No Hit
AATAGCAGGGGAAAGAAAAATTAAGAGGCATGCTAAAAATAATTGAGGTTTT850.36073505071510414No Hit
CTCGCTGGGGCAAATTGTGTATGGCAGCGAGTTACAAACCACAGAAAATGGG850.36073505071510414No Hit
TAACACGGGAGATGAAAATCTCTTGTTTTGAACGAAGATTTTAAAGAACTCA850.36073505071510414No Hit
GTGATCAATCTATTAAGAAATGTTTGCTCAGAAGCTGCTCAGAAACGTTCTC830.35224716716886645No Hit
GGGACTGGGTTTAAGTGATATGGCAGATGATTACATTTCTTACAATATTGAT820.3480032253957476No Hit
ATTATGGGGATACTGTAGAAACCAGCTCATGTGTACCTCATATCCCATCCTT810.3437592836226287No Hit
AATCGAGGGCCAAACCAGACAGACAGACAAAAAATTTTTTATTGCTAATGGT790.33527140007639095No Hit
GAAGAAGGGCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAA790.33527140007639095No Hit
GATTCCGGGGGCTTGGTCACTTCGTGGCTAAGGTAAGAACGTGCTTGTGGAA780.33102745830327207No Hit
GTCCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA780.33102745830327207No Hit
TAGGCCGGGATCTGACATGGTGCATCTGGAGACAACATCTGATGGGATCCAA750.31829563298391544No Hit
AGCTAGGGGTTTAGAGAGGGTTTGTTAAGGTTTATACTATTTTTGGATTGTG740.31405169121079657No Hit
TTAATGGGGTGACTTGAGTCCAGTGAAATCTCATTAGGGTTTAAGAATATTT740.31405169121079657No Hit
TAAGATGGGAATATATAAGCATTAACAACAACAACAAAAAATTTAAAACAAG730.30980774943767775No Hit
GGACAGGGGTTTACTACATATTGAAGGGTGTATTCACTTGGAGAAGTAGGAG690.29283198234520225No Hit
TTTTGCGGGCCATTTTGGCTTGATCTTAATCTTTTTTTCCCCCCTTTTCTCT680.28858804057208337No Hit
AGGTTAGGGGCTCAATACAGCAAGGACTTTGTTAGCTGTTTTTGTTCATATA670.2843440987989645No Hit
AGAATAGGGTTGATGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATG660.28010015702584556No Hit
ACTAATGAGCCTTATTCCATTTCCTGATGTTTTAAAAGAAGAAACACTGCCT640.27161227347960787No Hit
ACGAATGGGATTTTCGAACAAGAACATGTGATTTTTAAATCCATATATTGAA640.27161227347960787No Hit
GGACCAGGGGCTTCAGGGATTCCATAAACATTTAAACAATGTTCTAAGGTAC630.267368331706489No Hit
TTAACTGGGCATGGTTCTCAATTTATTTGGGGGGAAATACCTTGAGCAGAAT630.267368331706489No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT630.267368331706489No Hit
TAGACAGGGGTCAGAGCATTCTGTTATTCTTAACAAGTGTTCTGAAGAGCAA620.2631243899333701No Hit
GCGATGGGGTCTGTTATATTTGGACAATGAGTATTCCATGATTATTTATTGC600.25463650638713237No Hit
TGAATGGGGGTTGTCTGGTATCCTACTAGTGACAGGTCCTGTTTATCAGTTT600.25463650638713237No Hit
CTACTGGGGGATTCCCTGTTTAATAAATGGTGCTAGGAAAACTGGCTAGCCA590.2503925646140135No Hit
GATCATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA580.24614862284089464No Hit
CCATGAGGGTCTCCTGCCGGGATTACAACACTGTGGTGTTGTAAATTGTGGT580.24614862284089464No Hit
TGTTGTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT580.24614862284089464No Hit
ACCGTAGGGGTCCAGCAGATACTAGGTGCCTGGAATCATGATTGGCATTAGG570.24190468106777574No Hit
GTTAATGGGTATACAAATATATACACACATACACCAATGCCCAAGTTCCACT570.24190468106777574No Hit
TAAACGGGGTCAAGGGGTCACTTGTGAAATTGTAAGTTTCATGGGTAGCGAA560.23766073929465686No Hit
CACGCCCGGGGATGCAAAAGCAAACTTCAGTCAGACCTGTATCCAATAAGTC560.23766073929465686No Hit
TAGACTGGGTGGCACCATTGACTTCCCCGAATTTTTGACTATGATGGCTAGA540.22917285574841914No Hit
GAAATGGGTATCAGCACAAACAAAACTTAAATCAGGGTACATGACATGAAGT520.22068497220218136No Hit
GAGGTGGGAGAATTGCTTGAGCCCAGGAGGTCGAGGCTGCAGTGAGCCATGA520.22068497220218136No Hit
TTTAGAGGGTTATGAGTGGAGAGACATGTACATGTTAAAAGCATGTTGCATT520.22068497220218136No Hit
GTCCAAGGGTAACCGGAAGGAAGACAAGGAGGAGGACAAATGTTAAAGAAGT510.21644103042906251No Hit
ATTCGAGGGGTCAAAGATGGGAAGAAAGATGAAAAACAAGAAATAGTTGTGT510.21644103042906251No Hit
AAAACAGGGTCTTGATGTTTAGAAAAGTATTCGTTACATCTTGTAGGATCTA500.21219708865594364No Hit
ACGAAGGGTGTGAATTTTAGATTTGGATGATCTCAACAAGTTTTCCCTTTTG500.21219708865594364No Hit
ATAATAGGGTACTGAATGGTAGAGATGGTTCTATACAGGATGCTTTAGGAAA490.2079531468828248No Hit
ACAAATGGGGGAGCTCAGCAAAGAGGCTACAGCTTTTTTGTTTCCACTTAGG480.2037092051097059No Hit
CCGAACGGGTGGATTCTAGATTAGATGACAGTGTTGTATCAATGATAAGTTA460.19522132156346816No Hit
ATACTAGGGTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCT460.19522132156346816No Hit
AATTGGGGGTATAACAGAGATGTTTAGATGCTGTCACTTCTACTAACATGGT460.19522132156346816No Hit
ATTGTGGGGACCCATTAAAAAGGAAGCAGAAATACTGAGACATGAAAGGCAT450.1909773797903493No Hit
GCCGGGGTGTGAAAGCAGAGAAATAACTTGTAAATGGATAATTTTGTTCTCT430.18248949624411154No Hit
TGTCATGGGGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCT430.18248949624411154No Hit
GGGCCGAGGATACCAAACATATTTATGAGATCTATTGTTACTTAAAAATACT420.17824555447099266No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTA420.17824555447099266No Hit
TACACTGGGCTTTAATTAAAATGATTGATAATAACCACTTTATTAAAAACCT420.17824555447099266No Hit
AAACCAAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT410.1740016126978738No Hit
ATGCTTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT410.1740016126978738No Hit
ATTTATGGGATCCAGACATGTGTCAGATTAGCAGTGGGCCAGAATTAAATTC410.1740016126978738No Hit
CGTATGGGGAAGATCTCTTGGTAACAATTGACATTGCACTAGAATTTGAATG410.1740016126978738No Hit
ATAGGAAGGGTTTGATTCTTGTTTTTCAGTGGTCTTGCCAGAACAAAGTTGC400.1697576709247549No Hit
TAGAAGGGTAAGGAATGAATTTGCATTTAAAATCTTAATGAATGTTTTCAAA400.1697576709247549No Hit
AATAATGGGGTATTCATAAATGTGTTTCACGTTTCTGAACCTGACATACTGT400.1697576709247549No Hit
TAGGCTAGGGGCCGGCACTGGGAGCAGCCGCCAGCAGGGAAATCTGGCCTGA390.16551372915163604No Hit
CAGGCCGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTA370.15702584560539828No Hit
GGCTATGGGTGCAGTGACTGCAGAATACACATTCTTCTCCTCAGCATATGGG370.15702584560539828No Hit
GTCTGCGGGTGAGGACTTCCTTGGCCTTAGATGAGTCCCTATTTAGAGGAAG360.15278190383227944No Hit
TAATATGGGGTCCCAGCTACTCGGGAGGCCGAGGCTGGAGGATTCCTTAAAC360.15278190383227944No Hit
GTTCCTGGGACTGATCTTGAGGAAAACCAGAATGCTTTGAAGTTCTGTAATT350.14853796205916053No Hit
AATTAGGGCCGCGCCGCCTCCGCCGTGCCGCCGACCACGATGTGGGCTCTGA340.14429402028604169No Hit
TTTCTAGGGTTTGAAAATCAATCTCATGGAAGTTTCTATTTGCTTTTATTGC340.14429402028604169No Hit
TTTGCAGGGAGCGAGAGGCAGCAAGGTGGCCACAGTGTCACTGGTCAGGTGC340.14429402028604169No Hit
GGTCTGGGGTTACTGCTTCCTCAAATCTTTCGTAAGCAACTTCCTCTCCTTG330.14005007851292278No Hit
TACGCAGGGCCATGAACTGTGGTAAGGAGATGGTGTTTAACAACGTGGACTC330.14005007851292278No Hit
ACCACAGGCTGGCATTTGAAAACTCCTCTTTGCCATACCCAACCTATCTCAA320.13580613673980393No Hit
GCTGACGGGCGAACAGCAAAGCATTCTGTAGAAAGAGGTGTGTAGGTGCTGG320.13580613673980393No Hit
ATAGCAGGGATGTACACCATAGGGCTCAGTTTCCCTGAGGAATTAAAGTCAT320.13580613673980393No Hit
AGGTACGGGGTGAGCCTGGCTGGGTCTGCAGATGGAAGGCCTTGTTCAAGTA320.13580613673980393No Hit
TCCCTGGGAAGTGCCAGGTTCAGGATTTAAAAGTTTTTGCTCTTACCTACTC310.13156219496668506No Hit
AATGCGGGGTGATGACCAGGCTGGGCACAGTGGCTCACGCCTATGATCCCAC310.13156219496668506No Hit
TCTAGCGGGTTTCCTTCTGTTTCCACTTCTGATGAGTTGTGGCAGCATTAAC310.13156219496668506No Hit
CGTCGTGGGCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAA300.12731825319356618No Hit
CACACTGGGATGGTTACTTGCTCGTGCGTTGCCACACTGTGTTATAGTTTGC300.12731825319356618No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCT300.12731825319356618No Hit
CTGAGAGGGGGGCTCACTGCAACCTCCACCTCTCAGGTTCAAGCGATTCTCC280.11883036964732843No Hit
GTAAGTGGGGAAGCTGTGATGGGAAGGAGGCCTGCACTTGGACCTGACCTCA280.11883036964732843No Hit
TTCCGGAGCTCCCCCACCCCCATCCCCTTCGAATCTCAGCTTGGCTGTTTCC270.11458642787420957No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTACTGTCTCTTA270.11458642787420957No Hit
ATTGCGGGGTCCGATCTACTGCAGGGATGTGTTAGCTGTGAGAATGACTTCT270.11458642787420957No Hit
CTATGGGGGATCATGATGGAATACCAAAAAGATATCCTGTGATAAAGAAATA260.11034248610109068No Hit
AAAAACGGGTATCTGTTAAAGATACTATGATTTATGGATTAAATGATAAAAT250.10609854432797182No Hit
TAACTAGGGTTAGCAGGCAACTGACTTCTCTGAGCCTCTGCCTGCTTTGTTG250.10609854432797182No Hit
GTGTGTGGGTCACAGGAAGATTCTTCTTCTGTTGCCTTATTGTTTTTTTTTT250.10609854432797182No Hit
ACTAGTGGGTATCTGCTTTGCTTATTTGTTGCATGGGCAGCTGGTATTTAAT240.10185460255485294No Hit
TTAATGGGGTTTGTGTCTTAGTTTTATATGCATCCTTATTTTTAATACACCT240.10185460255485294No Hit
GACCTGGGGCTGGGTGACAGAGCGAGACTCCGTCTCAAATAAAAAACAACAA240.10185460255485294No Hit
GTGACTGGGCTCCAATGGTTTAATGGGATAGAGGAATCAATGTTTATATTTT240.10185460255485294No Hit
ACCGGGGTCTGCGTCTGGGCTCACGCCTGTTATCCCAGTACTTTGTGAGGCC240.10185460255485294No Hit
CAATAGGGGACCTTATTGTACCTAAGGGATTCTAAAGGTGTTGTCACTGTAT240.10185460255485294No Hit
ACAGATGGGATATCTTAGTATATTATAGCACCTTTTATTTTAAATTCTCTAT240.10185460255485294No Hit
GCTAAGGGGCAGGAAAAACATTGAAAGTTTGACACCTTAGATCAAATGGACC240.10185460255485294No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTAACTCGTATGC240.10185460255485294No Hit
GTACAAGGGTGGTGTGTGATGTTCCCAAATTTTTTTTTTCTCATCAACATTA240.10185460255485294No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)