FastQCFastQC Report
Sat 31 Dec 2022
SRR5848892.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848892.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23267
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC4151.7836420681652125No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT3551.5257661065027721No Hit
CTTCAGGGGAGAAAATGCTCTATGTCACCAATTATTTGGGAGGTGCGGGTCG1840.7908196157648172No Hit
AAGCTCGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC1720.7392444234323291No Hit
ATGCCTGGGATGACATGATCTTATACCTAGAAAAGCCTAAAGGCTCCTCCAA1570.6747754330167189No Hit
ATCCTCGGGCTGCCAAAATTATGGATCCGGATGAGCAATTGGAAACCCTTCA1570.6747754330167189No Hit
TCGTACGGGTCGCGCTGGGACCATAAACACATCACCTTGATTTTTAATCCCA1570.6747754330167189No Hit
TCCACAGGGCAACTTCAGAATTCGAGGAAGATGATGTGAATCTTCCAATTTT1550.6661795676279709No Hit
TGATAGGGGATTCCTGAAGCTGACAGCATTCGGGCCGAGATGTCTCGCTCCG1550.6661795676279709No Hit
GTGCTAGGGCTCAAACAATGGATGACACAAAACTATGAGAGTGACAAAATGG1490.6403919714617269No Hit
CCAACCGGGATATGTAAATAGCTTCCCACTTCTGGACCCATCTGATGCACCT1480.6360940387673529No Hit
TCTCAGGGGTGTGATATATCTGTGCAATGGAATATTATTTGACAATAAAAAA1470.6317961060729789No Hit
GTCGCCGGGCACCCAGAGGCCTGCTCTCCTCACACATTGTGTGGTTTGGGGT1460.6274981733786049No Hit
TAGGTGGGGGGCCGCAGAAGGGGTCCTGGTGACGAGTCCCGCGTTCTCTCCT1450.6232002406842309No Hit
TTGCAGGGGAGACCAGCTGTATTTCTTTATAAACAACAACATCAGAGCATAT1430.614604375295483No Hit
ATTTTTGGGTATGAGAAGGCAAATGATGTGAGGATTAGTATCTTGAGATTCA1400.6017105772123608No Hit
AGCCACGGGCAAAGGGCGGTACCTGCAGATGAAGGCGAAGCGACAGGGCCAC1380.5931147118236129No Hit
AGTCAGGGGTCAGACTAACAGTGGACCTCTCTGCAGAAGCCCTACAAGCCAG1380.5931147118236129No Hit
ATTGTAGGGTCTGATTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAA1370.5888167791292388No Hit
CTTACTGGGACCCTGATCGCATCATGAATACCTTCAGTGTGGTGCCTTCACC1360.5845188464348648No Hit
GCGTATGGGACCCTTTTGCCTTTATTGTCGACGTGACTCACCAGACGTCGGG1330.5716250483517429No Hit
TGATGGGGTATAAAGAAAAACAAAAGTCTTTTTTTCTCAAGGATGTAAGAAT1320.5673271156573688No Hit
GTTCACGGGAAAATCAGAAAGTATGAGATCCTGTTATGTTAAGGGAAATCCA1270.5458374521854987No Hit
AGCCCTGGGAAGAAGGGATCTATCACCTCTGTACAGGCTATCTATGTGCCTG1250.5372415867967508No Hit
GTGAAGGGGTAAAGCACCAAGCAGGCTTTCAGGGTCCCTGATTTCAGGCCTT1250.5372415867967508No Hit
GGTTCCGGGCTGGCAACTGGTGCCAATGTTATTCTAACCACTGGTGGAATTG1240.5329436541023768No Hit
TTTTGCGGGGAGCAGCGAGCCTGCGTCTGTCTCAGCTGTCCAGCGCTGAGGG1230.5286457214080028No Hit
TAACACGGGAGATGAAAATCTCTTGTTTTGAACGAAGATTTTAAAGAACTCA1200.5157519233248807No Hit
GCCCCAGAGAAAGTCCTCGCTCGTCACCAGCAAGCTTGCGGGTGGCCAAGTT1150.4942622598530107No Hit
AACACCGGGTACTTTGGATATGGATTTAAATGGTCTCTAAGAGCCGGGGGTA1130.4856663944642627No Hit
GGTTCTGGGTCACTGTCCATCTGCGTTCCCAGAGAACAATTGTGAAGAGTCC1100.47277259638114066No Hit
ATTCAAGGGACAGACAACATCTACTGAGCTAGTTGATTGGTGATGCTTCTAC1100.47277259638114066No Hit
GATTCAGGGCAGATCCTGGAGATTGTCCTGGCCTTTGGCAACTACATGAACA1090.4684746636867666No Hit
AATATTGGGAATAACAACTAGAAAAGGCAGTATACTTCACTGATGCTTGTCT1070.4598787982980187No Hit
AAACCAGGGCTAACTTCTCCAAGGGCACTGGCAAAGGAGATGTACAGAAGAC1070.4598787982980187No Hit
TAAGGTGGGACGACACGTTACTAGCAGTCACTTGATCTCCGTGGCTTTGGTT1040.4469850002148966No Hit
GTTACAGGGTGTATGGGTTTATTTCTGGACTCTCAATTCTATTCATTTGATC1030.4426870675205226No Hit
CCCATAGGGACTCTCTTTGATCTCTATACAAGAAAGCTGGAAATTAAAAACA1020.43838913482614866No Hit
AATGCAGGGACACTCGTCCCCAGAGGCACATTCGTGCACATTCTCACAGACA1010.4340912021317746No Hit
ACCAATGGGTCCATTAGTAGACTTTCTGGGTAATTTGTCTTTTTAAAAATTA1010.4340912021317746No Hit
ATCATCGGGGAGTATTTCTTCTGAAGTAGATGTAATCACTTCCTCTCTAGCA1000.4297932694374006No Hit
CTAGAGGGGCTGAGTCCTTGACGCTGGCTCTGTCCCAGGATCTCCACAGACC980.4211974040486526No Hit
GTGAAGGGTAACAAACTATCTCTCAGACCACAGTGCAATCAAACTAGAACTG980.4211974040486526No Hit
TCTCATGGGTGCCTTCCCTAATAAGGACATGAAACTGGAGAACCACAAACCA970.41689947135427863No Hit
ATTATGGGGATACTGTAGAAACCAGCTCATGTGTACCTCATATCCCATCCTT960.41260153865990457No Hit
CGTAATGGGTGACCAGTACACAATTCATTCTACCTGTGAGAGTATAAGAGTT950.40830360596553056No Hit
AGGAAAGGGTGGAAAATGAAAATAGGGAAAATAATTGAATCATTTTAGAAGT940.4040056732711566No Hit
TGGCTGGGTGAACCAGAGGCAAGAACACCAATTGGAAGGCTGATGCTGTCAT940.4040056732711566No Hit
ATGAAAGGGTAACTGAGTAAAGCAGAAACATGTTAAGTAGAGACATGAAAGA930.39970774057678254No Hit
CCATCAGGGTGTGGAGAATCATCAAGATATGAATTGAATCGCTGCGATACTG900.3868139424936605No Hit
TGCACTGGGGCGAAAAAGGCGCTGGAGCGTGCGCGCCAGGTGCAGCTCGCCG890.3825160097992865No Hit
AGACTGGGGGTTAAGATGGCAGAGCCCGGTAATCGCATAAAACTTAAAACTT860.36962221171616455No Hit
AATCGAGGGCCAAACCAGACAGACAGACAAAAAATTTTTTATTGCTAATGGT830.35672841363304253No Hit
CTCGCTGGGGCAAATTGTGTATGGCAGCGAGTTACAAACCACAGAAAATGGG810.3481325482442945No Hit
GTGATCAATCTATTAAGAAATGTTTGCTCAGAAGCTGCTCAGAAACGTTCTC810.3481325482442945No Hit
TAGACAGGGGTCAGAGCATTCTGTTATTCTTAACAAGTGTTCTGAAGAGCAA800.34383461554992045No Hit
GATTCCGGGGGCTTGGTCACTTCGTGGCTAAGGTAAGAACGTGCTTGTGGAA800.34383461554992045No Hit
ACTAATGAGCCTTATTCCATTTCCTGATGTTTTAAAAGAAGAAACACTGCCT770.3309408174667985No Hit
AACAAGGGGTGTGCAGCCAAAGTTGAGAGCTAGGGAACAGATGGGGGTGGGG770.3309408174667985No Hit
GATCATGGGATAGGTTTGGTCCTAGCCTTTCTATTAGCTCTTAGTAAGATTA770.3309408174667985No Hit
GGTGATGGGCAGAGAAAGTGGTATACTGAAAGTTTTGATTAGATTTTATAAG760.3266428847724245No Hit
AATAGCAGGGGAAAGAAAAATTAAGAGGCATGCTAAAAATAATTGAGGTTTT750.3223449520780504No Hit
AGAATAGGGTTGATGTTTACAGGCCTGCTTTTTGCTTATAATTGACAACATG720.30945115399492845No Hit
TAAGATGGGAATATATAAGCATTAACAACAACAACAAAAAATTTAAAACAAG710.30515322130055444No Hit
GGACAGGGGTTTACTACATATTGAAGGGTGTATTCACTTGGAGAAGTAGGAG700.3008552886061804No Hit
GAAGAAGGGCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCGTAAA680.2922594232174324No Hit
CCGAACGGGTGGATTCTAGATTAGATGACAGTGTTGTATCAATGATAAGTTA670.2879614905230584No Hit
GAACAAGGGAAGGACTTAAGTGTCTCCTCCAACAGTACGAGATAATTATGTA670.2879614905230584No Hit
TTTTGCGGGCCATTTTGGCTTGATCTTAATCTTTTTTTCCCCCCTTTTCTCT670.2879614905230584No Hit
GGGACTGGGTTTAAGTGATATGGCAGATGATTACATTTCTTACAATATTGAT670.2879614905230584No Hit
TTAATGGGGTGACTTGAGTCCAGTGAAATCTCATTAGGGTTTAAGAATATTT670.2879614905230584No Hit
GGGCCGAGGATACCAAACATATTTATGAGATCTATTGTTACTTAAAAATACT670.2879614905230584No Hit
TAAACGGGGTCAAGGGGTCACTTGTGAAATTGTAAGTTTCATGGGTAGCGAA670.2879614905230584No Hit
ACGAAGGGTGTGAATTTTAGATTTGGATGATCTCAACAAGTTTTCCCTTTTG660.2836635578286844No Hit
AGCTAGGGGTTTAGAGAGGGTTTGTTAAGGTTTATACTATTTTTGGATTGTG650.2793656251343104No Hit
CTACTGGGGGATTCCCTGTTTAATAAATGGTGCTAGGAAAACTGGCTAGCCA630.2707697597455624No Hit
GTCCACGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA630.2707697597455624No Hit
ACAAATGGGGGAGCTCAGCAAAGAGGCTACAGCTTTTTTGTTTCCACTTAGG620.2664718270511884No Hit
GCGATGGGGTCTGTTATATTTGGACAATGAGTATTCCATGATTATTTATTGC610.2621738943568144No Hit
TAGGCCGGGATCTGACATGGTGCATCTGGAGACAACATCTGATGGGATCCAA600.25787596166244037No Hit
GGCTATGGGTGCAGTGACTGCAGAATACACATTCTTCTCCTCAGCATATGGG590.25357802896806636No Hit
ACCGTAGGGGTCCAGCAGATACTAGGTGCCTGGAATCATGATTGGCATTAGG590.25357802896806636No Hit
GTTAATGGGTATACAAATATATACACACATACACCAATGCCCAAGTTCCACT590.25357802896806636No Hit
ATACTAGGGTTCAAGGAGAATGGCTATGTGACCATGTCGGTGGGAAAAGTCT580.24928009627369235No Hit
TGAATGGGGGTTGTCTGGTATCCTACTAGTGACAGGTCCTGTTTATCAGTTT580.24928009627369235No Hit
CAGGCCGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTTA560.24068423088494437No Hit
TAGACTGGGTGGCACCATTGACTTCCCCGAATTTTTGACTATGATGGCTAGA560.24068423088494437No Hit
GAGGTGGGAGAATTGCTTGAGCCCAGGAGGTCGAGGCTGCAGTGAGCCATGA550.23638629819057033No Hit
TGTCATGGGGTGATGAGGGGATCCAGAGAGTGCTCACCACAGACTACGCGCT550.23638629819057033No Hit
TTAACTGGGCATGGTTCTCAATTTATTTGGGGGGAAATACCTTGAGCAGAAT540.23208836549619632No Hit
AATAATGGGGTATTCATAAATGTGTTTCACGTTTCTGAACCTGACATACTGT540.23208836549619632No Hit
GCCGGGGTGTGAAAGCAGAGAAATAACTTGTAAATGGATAATTTTGTTCTCT540.23208836549619632No Hit
AAACCAAGGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT530.22779043280182232No Hit
TTTAGAGGGTTATGAGTGGAGAGACATGTACATGTTAAAAGCATGTTGCATT530.22779043280182232No Hit
AAAACAGGGTCTTGATGTTTAGAAAAGTATTCGTTACATCTTGTAGGATCTA520.2234925001074483No Hit
ACGAATGGGATTTTCGAACAAGAACATGTGATTTTTAAATCCATATATTGAA520.2234925001074483No Hit
GAAATGGGTATCAGCACAAACAAAACTTAAATCAGGGTACATGACATGAAGT510.21919456741307433No Hit
GTCCAAGGGTAACCGGAAGGAAGACAAGGAGGAGGACAAATGTTAAAGAAGT500.2148966347187003No Hit
TACACTGGGCTTTAATTAAAATGATTGATAATAACCACTTTATTAAAAACCT490.2105987020243263No Hit
ATGCTTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT480.20630076932995228No Hit
ATAGCAGGGATGTACACCATAGGGCTCAGTTTCCCTGAGGAATTAAAGTCAT480.20630076932995228No Hit
ATAATAGGGTACTGAATGGTAGAGATGGTTCTATACAGGATGCTTTAGGAAA470.2020028366355783No Hit
TAGAAGGGTAAGGAATGAATTTGCATTTAAAATCTTAATGAATGTTTTCAAA460.1977049039412043No Hit
TGTTGTGGGGCTTTTAGATTAAAATGAAGGTGACTTAAACAGCTTAAAGTTT460.1977049039412043No Hit
GGACCAGGGGCTTCAGGGATTCCATAAACATTTAAACAATGTTCTAAGGTAC450.19340697124683026No Hit
AATTGGGGGTATAACAGAGATGTTTAGATGCTGTCACTTCTACTAACATGGT450.19340697124683026No Hit
ATTGTGGGGACCCATTAAAAAGGAAGCAGAAATACTGAGACATGAAAGGCAT450.19340697124683026No Hit
AGGTTAGGGGCTCAATACAGCAAGGACTTTGTTAGCTGTTTTTGTTCATATA440.18910903855245625No Hit
ACCGGGGTCTGCGTCTGGGCTCACGCCTGTTATCCCAGTACTTTGTGAGGCC430.18481110585808228No Hit
TAGGCTAGGGGCCGGCACTGGGAGCAGCCGCCAGCAGGGAAATCTGGCCTGA410.17621524046933426No Hit
ATTCGAGGGGTCAAAGATGGGAAGAAAGATGAAAAACAAGAAATAGTTGTGT410.17621524046933426No Hit
CCATGAGGGTCTCCTGCCGGGATTACAACACTGTGGTGTTGTAAATTGTGGT410.17621524046933426No Hit
ACCACAGGCTGGCATTTGAAAACTCCTCTTTGCCATACCCAACCTATCTCAA400.17191730777496023No Hit
CACGCCCGGGGATGCAAAAGCAAACTTCAGTCAGACCTGTATCCAATAAGTC400.17191730777496023No Hit
CGTATGGGGAAGATCTCTTGGTAACAATTGACATTGCACTAGAATTTGAATG400.17191730777496023No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT390.16761937508058625No Hit
ATTTATGGGATCCAGACATGTGTCAGATTAGCAGTGGGCCAGAATTAAATTC380.16332144238621224No Hit
CTGAGAGGGGGGCTCACTGCAACCTCCACCTCTCAGGTTCAAGCGATTCTCC360.15472557699746423No Hit
AATGCGGGGTGATGACCAGGCTGGGCACAGTGGCTCACGCCTATGATCCCAC360.15472557699746423No Hit
GAATAGGGTCCCAGCTATTCACGAGGCTGAGGCAGGGAATTGCTTGAACCCT360.15472557699746423No Hit
TCCCTGGGAAGTGCCAGGTTCAGGATTTAAAAGTTTTTGCTCTTACCTACTC350.1504276443030902No Hit
CTATGGGGGATCATGATGGAATACCAAAAAGATATCCTGTGATAAAGAAATA350.1504276443030902No Hit
TCTAGCGGGTTTCCTTCTGTTTCCACTTCTGATGAGTTGTGGCAGCATTAAC350.1504276443030902No Hit
GCTAAGGGGCAGGAAAAACATTGAAAGTTTGACACCTTAGATCAAATGGACC350.1504276443030902No Hit
ATGATAGGGGTTATTAAGCATTTGATTTTCTGTGTCCTTAAGTACTTCCTGA340.1461297116087162No Hit
CGTCGTGGGCTATGAGCCTAATGACCACAGCAGGTATACAGCTCTGGCCCAA330.1418317789143422No Hit
TACGCAGGGCCATGAACTGTGGTAAGGAGATGGTGTTTAACAACGTGGACTC330.1418317789143422No Hit
TACGCTGGGGTTTACCAATCAACAGCATGTCTACTGCTTGGATCCATACAAA330.1418317789143422No Hit
AGGTACGGGGTGAGCCTGGCTGGGTCTGCAGATGGAAGGCCTTGTTCAAGTA330.1418317789143422No Hit
CACACTGGGATGGTTACTTGCTCGTGCGTTGCCACACTGTGTTATAGTTTGC320.1375338462199682No Hit
ACAGATGGGATATCTTAGTATATTATAGCACCTTTTATTTTAAATTCTCTAT310.1332359135255942No Hit
TTTGCAGGGAGCGAGAGGCAGCAAGGTGGCCACAGTGTCACTGGTCAGGTGC310.1332359135255942No Hit
GTAAGTGGGGAAGCTGTGATGGGAAGGAGGCCTGCACTTGGACCTGACCTCA310.1332359135255942No Hit
GTACAAGGGTGGTGTGTGATGTTCCCAAATTTTTTTTTTCTCATCAACATTA300.12893798083122018No Hit
TTCCGGAGCTCCCCCACCCCCATCCCCTTCGAATCTCAGCTTGGCTGTTTCC290.12464004813684618No Hit
GTGACTGGGCTCCAATGGTTTAATGGGATAGAGGAATCAATGTTTATATTTT290.12464004813684618No Hit
TAACTAGGGTTAGCAGGCAACTGACTTCTCTGAGCCTCTGCCTGCTTTGTTG290.12464004813684618No Hit
TTTCTAGGGTTTGAAAATCAATCTCATGGAAGTTTCTATTTGCTTTTATTGC290.12464004813684618No Hit
AAAAACGGGTATCTGTTAAAGATACTATGATTTATGGATTAAATGATAAAAT280.12034211544247218No Hit
CAATAGGGGACCTTATTGTACCTAAGGGATTCTAAAGGTGTTGTCACTGTAT280.12034211544247218No Hit
GACCTGGGGCTGGGTGACAGAGCGAGACTCCGTCTCAAATAAAAAACAACAA270.11604418274809816No Hit
TAATATGGGGTCCCAGCTACTCGGGAGGCCGAGGCTGGAGGATTCCTTAAAC270.11604418274809816No Hit
GCTGACGGGCGAACAGCAAAGCATTCTGTAGAAAGAGGTGTGTAGGTGCTGG270.11604418274809816No Hit
ATTGCGGGGTCCGATCTACTGCAGGGATGTGTTAGCTGTGAGAATGACTTCT270.11604418274809816No Hit
GTCTGCGGGTGAGGACTTCCTTGGCCTTAGATGAGTCCCTATTTAGAGGAAG260.11174625005372416No Hit
GGTCTGGGGTTACTGCTTCCTCAAATCTTTCGTAAGCAACTTCCTCTCCTTG260.11174625005372416No Hit
TGCATTGGGTAACAATCTCAGTTTCCTTTTAAAAAAAGAAAGAAAGGAAAAG260.11174625005372416No Hit
ATCTGAAGACTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTA260.11174625005372416No Hit
ATCTGAAGCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTAT260.11174625005372416No Hit
ATAGGAAGGGTTTGATTCTTGTTTTTCAGTGGTCTTGCCAGAACAAAGTTGC240.10315038466497614No Hit
CCAGCAGGGCAGCCTCAGTGACAGAGTGAGGCCCTGTCTTTAAATAAATAAA240.10315038466497614No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCT240.10315038466497614No Hit
ACACGCGGGAGATTCAACAATCTTATAGTAAAAGCAGCCATAGACTAAATAT240.10315038466497614No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)