FastQCFastQC Report
Sat 31 Dec 2022
SRR5848866.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848866.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121376
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5650.46549564988136044No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT5650.46549564988136044No Hit
GAAGTTGGGAGCTGTTTATAGTGCTCATTTAGGCACACCTAAAGTGAAATAA1760.14500395465330873No Hit
GACAGGGGGATTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAA1710.14088452412338517No Hit
TTTACGGGATCAAAACAAGGTCACTGCTACCTTCTCTCTCCATTCCATCGAG1620.1334695491695228No Hit
CCAGCAGGGGAAAGAAAAATTATAAGAGATTTGACTGAAGCAAGGAAAAAAA1560.12852623253361456No Hit
TCAAATGGGTGGTAATTGTTAGCATTTGCTAATAATTAACTTCTCGTCAAGA1520.12523068810967572No Hit
AAGACAGGGCTTGCTCACTCATCCACTTGAAGTAAGAAAAATCAATCACTGC1520.12523068810967572No Hit
CAGGGCAGCAGTACCAGGACGCCACGGCCGAGGAGGGCGAGTTCGAGGAGGA1500.1235829158977063No Hit
ATGCGTGGGAGTACTCCATTGTTTTTGCTATTTAAATATAATGTATCAAAAA1500.1235829158977063No Hit
GACAGGGATCTGAAAATAAAATTAAAAGCATAATTAGGCTTACAAACAAGAA1490.12275902979172158No Hit
TCTAATGGGATGTGATTACTTGGCATCTTCATAGCATGGTGGCTGGGCTTCA1480.1219351436857369No Hit
CATCTAAGGGGCACTTCCTTTATGTAGATCTGAGTTTCTGACATATAGTTTA1450.11946348536778276No Hit
CGTTTAGGGGCTTCCTGTCTGTACCAGGGCGGCGCGTGGTCTACGCCGAGTG1420.11699182704982863No Hit
GCCATGGGAAAAAATTCTCCAGCACCAACATGATGTAATTGGAAGAACATAG1420.11699182704982863No Hit
TGCAACGGGTCTGCAGATTCAATGCAATCCCTGTCAAAATACCAATGACTTT1390.11452016873187451No Hit
ATGATAGGGGGCTTTAAGAAAAAAGTGTAAACAGCTTTTTAAATTTTCTGTA1390.11452016873187451No Hit
AGCCTTGGGATGGCAGTGTTTGGGACTGTTTTGGCAGGTTAAGGAGTGGCAT1390.11452016873187451No Hit
AAAGCTGGGCAACAAAGGGTCGAGGGTTTGAGGCTTAAACAAGCCAACATAT1380.1136962826258898No Hit
ATTGAAGGGACCTCAGTGCCATGATAGAAACATTTCATCATGAAAACCTAAG1380.1136962826258898No Hit
TTGTCTGGGGCCGTGCAGAAGCTCTTTAGTTTAATTAGGTCCCATTTGTCAA1370.1128723965199051No Hit
AGACATGGGCCTGAATAAGAATTGCACGAGACTCTTGTCTGGAAAGAATTAT1360.11204851041392039No Hit
CTCGAGGGAATTTAGATGACTAAAAGAGATTGATACTTCCAAATAACTTGAG1360.11204851041392039No Hit
GGCCTTGGGGGGGTTCCAGGAGAGAGGAAAGATATTTGTTACAATAATGGTT1360.11204851041392039No Hit
TAGTTGGGGATCATTTTCTGGAAATATTAGAAATATGTACAAATCGAATCAA1350.11122462430793567No Hit
TTTGCCGGGAATGACTGGAAGTTAGATGTTGCAACAGATAATTTTTTCCAAA1350.11122462430793567No Hit
CAACTTGGGCGATCTCCGTGCGGGTGTTCTGAAATATTTTCTCCCTTTGGTG1350.11122462430793567No Hit
ATTAATGGGTAAGTTGTGATTTTTATGGTTGGAACCAAAGTTATTCAAATAG1350.11122462430793567No Hit
GCTCTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.11040073820195097No Hit
TAGCCTGGGGTGCCCCAATATCATGCTCAATAACTGTTTGCTAAAAAAAATG1340.11040073820195097No Hit
ACCGCAGGGCACAAACAGAGGGTGGCAGAAGTGCTCAATGACCCTGAGAACA1340.11040073820195097No Hit
GCACAGGGACATACATATCTTCTTTTAAATTTTTGTGGGTTAATTTGTAAAT1340.11040073820195097No Hit
AAGCAGGGGGTCCGAGACCTTCAAGGTAACCCAATTGCGAATGCCACCATCT1330.10957685209596625No Hit
GCAGGGGGTTCCGTATTTCTATATTTTCTCCTTTACAGTGAACCATACTTGG1330.10957685209596625No Hit
AAAACGGGGTGGGAATTCATGAGGTTTGAATTATATTATGTTTCTCTTGGTG1330.10957685209596625No Hit
ACATAAGGGCTGTTAGGGATTTAAATCAGCTATGAAAAATCTGCTCATTTTA1330.10957685209596625No Hit
GTTACGGGGCTGAGCTGGGAAGTACTTAAGCCACCCTGGTTAACTTCAGAAG1320.10875296598998153No Hit
CAATTTGGGATTAAAATATAAATATTCTATTAGCAAAAATATATTCTGTTCA1320.10875296598998153No Hit
GCGCAGGGGAGGCTTTTACCTTTATTGCTTTAGCATAAATTTGGTTTACTGA1310.10792907988399685No Hit
TCACAAGGGACCTTCTGATGTTGTAACTGCCGTAAGAGGAAAAGGATTATTA1310.10792907988399685No Hit
TATTCAGGGAGACATTTCTCTAAAGACATACATGTAGTCAAAAAACCCACGA1310.10792907988399685No Hit
CTCTACGGGAAGTCTTAACAATGTTTTCTAAGAGCAATAGCCCTGATGCAGT1310.10792907988399685No Hit
AAAGCTGGGCTAACAATCAGTACTTTTCTTCAGATGCTTTGTTCTGTTTAGA1300.10710519377801213No Hit
ATTAGCGGGGTCTTTAGCTGTGTTTATTAACTTCTCCGGTCTTGTTTTGTTT1290.10628130767202741No Hit
GTTGTCGGGTGTTTTAAACACTCACTTTATTTTCCTTTTGTTGGAGAATAAA1290.10628130767202741No Hit
AGAATTGGGAGGAGTGACTTGTAATTCTCATATGTTAGAAAGGCAGATCTCC1290.10628130767202741No Hit
TTGCAGGGGGAGATACTTTTCTTCTGCTTTCACTACTTAACACTTCTAAAAC1280.10545742156604272No Hit
TTACCAGGGATTTCATTGTATGAATGTACCACAATCTGTTTATCCACCCGCC1270.104633535460058No Hit
TGTAAAGGGGTGTCACCTCAATATGAAAATTCTGGCATTGTGTTTATGACTC1270.104633535460058No Hit
TCAGCACGGGAACCTATTTTGTAATAATGTGTTGAATATCTCATGTAATTTA1270.104633535460058No Hit
TAACCTAGGCCTAAGAAATAATGGGAAAAGAATCTCTGGATGTAAGTTTTTC1270.104633535460058No Hit
CTAACAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA1270.104633535460058No Hit
AGCAACGGGAAGAGACAATGTTTATCAATTCTGGCAGAATCTGTTTTACAAT1260.10380964935407329No Hit
ACAAGCCGGGGTGAAAGGAAGAGTCAATCAGGATGATAAACTTTGTCTTATT1260.10380964935407329No Hit
GCGCAAGGGTAGCATCATGTCTTTAGAGAAAGGTCAGCCTCTCTGGTTGCCA1260.10380964935407329No Hit
CTCGTAGGGCATATCAAAAAGATAATCCACTATGATCAAGTGGGTTTCATAC1260.10380964935407329No Hit
CACTTGGGGACGCTCTTCCGATCTACAAGTGGGGAAGTCCTCTTCTGGAAGC1250.1029857632480886No Hit
CCAGGCTGGGTGATACAGCAAGACCCTGTCTCAAAACAAACAAACAAACAGA1250.1029857632480886No Hit
TTTTACGGGAGAACTACTTAGCAGAGAACAGAAATATGAAGATGGAATTGCT1250.1029857632480886No Hit
ATGTTAGGGGTTGTCCAGCCTGGTCTCGAACTCCTGGCCTCAAGTGATCAGC1250.1029857632480886No Hit
ATAATAGGGGGGTGTGCTGATTTTAAAAGGCTTGAATAAAACATAAATTTCC1250.1029857632480886No Hit
TAGTCCGGGGAATACATGAAGGCTATTGAGTTTTTGTATGCTGATTTTATAA1240.10216187714210388No Hit
TACTTAGGGAGGTATCCAGCCTTTATCATATAAGCATTGACATTATCCAGGC1240.10216187714210388No Hit
GAGTATGGGCCTAGCAGGGATTCCAATGTGTTTATTCAGAATTTTCTTGAAA1240.10216187714210388No Hit
CACATGGGGACTTGTCTTTTCCTTTTAATTTCCCTTACTTATAAAGTGTTGG1240.10216187714210388No Hit
TTCGGCGGGGTTTCTCATGATGACTCAGTTTTCCTAACATTTCTCCATCCAA1230.10133799103611917No Hit
GGAGGTGGGGATTGGAAGGGTTAAGGTGGAAAAATAAAAAGATTTTGAAGGT1230.10133799103611917No Hit
CATACAGGGAGACCAACCTGGTGCCCTACCCCCGCATCCACTTCCCTCTGGC1230.10133799103611917No Hit
TTACAAGGGGAAAAATGAGGTAAGAGCTCTTACAGCCTTCTAATAACAATAT1230.10133799103611917No Hit
GCTTGGGGGGAGGGAGCCGGAGGGGACGCGCCGGAGGAAAGATGGAAGACTA1220.10051410493013446No Hit
CGTGCAGGGAAAGACAGGCAATTGAAATGAAAAATAGTGTTAGTAACAAAAA1220.10051410493013446No Hit
AACGCCGGGCAGTTGCCAGAGTGTGCCAGATACTGGGGTCGTCCTTAAAGCA1220.10051410493013446No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)