FastQCFastQC Report
Sat 31 Dec 2022
SRR5848865.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848865.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119465
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5860.4905202360524003No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT5500.46038588707989786No Hit
GAAGTTGGGAGCTGTTTATAGTGCTCATTTAGGCACACCTAAAGTGAAATAA1750.14648641861633116No Hit
GACAGGGGGATTTAGCAGCCGTGGCAACTGCCAAGGACACATACAACAAAAA1600.13393043987778847No Hit
GCGCAGGGGAGGCTTTTACCTTTATTGCTTTAGCATAAATTTGGTTTACTGA1530.12807098313313525No Hit
CTCGTAGGGCATATCAAAAAGATAATCCACTATGATCAAGTGGGTTTCATAC1520.12723391788389904No Hit
TAGTCCGGGGAATACATGAAGGCTATTGAGTTTTTGTATGCTGATTTTATAA1480.12388565688695435No Hit
TTCGGCGGGGTTTCTCATGATGACTCAGTTTTCCTAACATTTCTCCATCCAA1440.12053739589000963No Hit
ACTCAAGGGAGGTCTCTGAAGAACTGCTGGTGTTTAGGAATAAGAATGTGCA1430.11970033064077346No Hit
AAAGCTGGGCAACAAAGGGTCGAGGGTTTGAGGCTTAAACAAGCCAACATAT1420.11886326539153726No Hit
CATACAGGGAGACCAACCTGGTGCCCTACCCCCGCATCCACTTCCCTCTGGC1380.11551500439459256No Hit
AAAACGGGGTGGGAATTCATGAGGTTTGAATTATATTATGTTTCTCTTGGTG1380.11551500439459256No Hit
GACAGGGATCTGAAAATAAAATTAAAAGCATAATTAGGCTTACAAACAAGAA1350.11300380864688402No Hit
TACTTAGGGAGGTATCCAGCCTTTATCATATAAGCATTGACATTATCCAGGC1350.11300380864688402No Hit
AGATCAGGGTTCAATAAAATAGGTGAGTACTCAGGACTTTTCATTTTTCTCA1350.11300380864688402No Hit
ACATAAGGGCTGTTAGGGATTTAAATCAGCTATGAAAAATCTGCTCATTTTA1350.11300380864688402No Hit
AATTGTGGGAGAGTTACACATCCTCTCACCCGACATGTTGTAGGAACACATA1340.11216674339764784No Hit
GTAGATGGGATAAGTAGAAGGAATGAACAAATTCTTTTGAAACTATAAAGTA1330.11132967814841167No Hit
CAGGGCAGCAGTACCAGGACGCCACGGCCGAGGAGGGCGAGTTCGAGGAGGA1320.1104926128991755No Hit
AGACATGGGCCTGAATAAGAATTGCACGAGACTCTTGTCTGGAAAGAATTAT1320.1104926128991755No Hit
TTGTCTGGGGCCGTGCAGAAGCTCTTTAGTTTAATTAGGTCCCATTTGTCAA1310.1096555476499393No Hit
AGTCGGGGCGGAGATATCCATCACAAATTGCTTTTCATATATGACAAGAAAA1310.1096555476499393No Hit
TCAAATGGGTGGTAATTGTTAGCATTTGCTAATAATTAACTTCTCGTCAAGA1310.1096555476499393No Hit
GTGAGTGGGTTATGAAGTTAGTATTTGAGCCCCATAAGATCTTTAAAAAGCC1280.10714435190223079No Hit
AAGACAGGGCTTGCTCACTCATCCACTTGAAGTAAGAAAAATCAATCACTGC1280.10714435190223079No Hit
ATGCGTGGGAGTACTCCATTGTTTTTGCTATTTAAATATAATGTATCAAAAA1280.10714435190223079No Hit
CCAGCAGGGGAAAGAAAAATTATAAGAGATTTGACTGAAGCAAGGAAAAAAA1270.1063072866529946No Hit
TGTAAAGGGGTGTCACCTCAATATGAAAATTCTGGCATTGTGTTTATGACTC1270.1063072866529946No Hit
ACACCTGGGGGAGACCATGACAAAGTAAGAGACACTTATGAGGTATTTCCAG1270.1063072866529946No Hit
GCGCAAGGGTAGCATCATGTCTTTAGAGAAAGGTCAGCCTCTCTGGTTGCCA1270.1063072866529946No Hit
AGAATTGGGAGGAGTGACTTGTAATTCTCATATGTTAGAAAGGCAGATCTCC1270.1063072866529946No Hit
TGTAACGGGATTCTAACTGGTGTGAGATGGTATCTCATTGCGGTTTTGATTT1250.10463315615452223No Hit
GCACAGGGACATACATATCTTCTTTTAAATTTTTGTGGGTTAATTTGTAAAT1240.10379609090528606No Hit
GTTACGGGGCTGAGCTGGGAAGTACTTAAGCCACCCTGGTTAACTTCAGAAG1230.10295902565604989No Hit
TCTAAAGGGATACACCAATAACAGACAAACAGAGAGCCAAATCATGAGTGAA1230.10295902565604989No Hit
GATGTGGGGGAGGAAAGACATTCGTAATAGCCCCAAACTAGAAACAACCCAG1230.10295902565604989No Hit
TTTACGGGATCAAAACAAGGTCACTGCTACCTTCTCTCTCCATTCCATCGAG1220.10212196040681372No Hit
ACACGCGGGTTGCTTGCTGTGGACTTGGTGGAGGACTCAGGGGAGGAAAGGC1220.10212196040681372No Hit
TTGTTAGGGATTAATGAAATAGGAAAGAAGCATGTAATAAAAAGGATCAAAA1210.10128489515757755No Hit
GTCTAAGGGTTAAAAGCCAGATCTGTGACTTATAACCATTTCTGTATATCTG1210.10128489515757755No Hit
CAACCTTGGGATCCAGAGATGAAGAGACAGTCAACCAAGTCCATCAGGCTCT1200.10044782990834135No Hit
TTTAGTGGGACATTACTAATAACAATAGCTACCATTTATTTCATGCTTATCA1200.10044782990834135No Hit
GTTGTCGGGTGTTTTAAACACTCACTTTATTTTCCTTTTGTTGGAGAATAAA1200.10044782990834135No Hit
TGCCACCGGGGCAGGAAGGAGAAAAGGTGTTCGAGGTTTGAAGAGACAGGGG1200.10044782990834135No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)