FastQCFastQC Report
Sat 31 Dec 2022
SRR5848851.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848851.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120950
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT6620.5473336089293096No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5930.49028524183546923No Hit
TTAAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2040.16866473749483257No Hit
CATCTAGGGGAACGCGGGGCGAGCGGAGCCGGCTGAGCGGGCGCCGAGCTCC1560.12897891690781316No Hit
GCGTAATGGGGTGAATAAAAATAATTTTAGAAAAGTTATCTATTTTTATTGT1490.12319140140553948No Hit
AAAAACGGGCAACACCCTCATACCAAAGCCAGATAAGGACACTACAAGAAAA1480.12236461347664325No Hit
TAAGCGGGGGGATATGAAAGGATGTGTCTTATTGTTTTATGCGTTAAGGATA1480.12236461347664325No Hit
ATTGCTGGGGCAAGCAAACAGGCGATTACAGTGCAGGGGACTCGCGCAGCAG1470.12153782554774699No Hit
ACTAAGGGTATAAGAGAAGAAATATTGGAATACTGCATTTGTGGTGGAAGGT1460.12071103761885076No Hit
TTCGTTGGGGATCACTTGAGATCAGGACTTTAAGACCAGCCTCGGCAACAAC1440.11905746176105829No Hit
GCAGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1380.11409673418768086No Hit
CTCAACGGGCCGATCTGTGAAGGGCATCTAAGGCAGCAATCCTCAAATGCTA1370.11326994625878463No Hit
CATAGGGAAGATCACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCTAC1370.11326994625878463No Hit
ATTCTAGGGAACCTCCCACCTAACTTAAGACTCATCTTGAAACCATCCCTAT1360.11244315832988838No Hit
CCGTATGGGATATGTCTTTGTATTACATCCAGCCAGCCTCAGAGGCTGGCCC1330.10996279454319967No Hit
ATATCCGGGGGAAGGGAGCGGCGTGCCGTCCGGGTCGCCTAGGCCTGGGGTC1320.10913600661430344No Hit
TTGGCGGGGACAGATTTGTATTCTCATTCTAGAATGACCATTTACAAGTTGT1310.10830921868540719No Hit
TGCACGCGGATTGTAGAGACACCACCCATGGTGGTTGTGGGCATTGTGGGCT1310.10830921868540719No Hit
AGTTTAGGGTCAGAATGACAGGGCAGTGGATTACTGTTTCTGGATCACCAGA1300.10748243075651095No Hit
TGAAGTAGGGACGCTCTCCGATCTTCAATGGGACGGGGATGGTGGAAGAGGG1300.10748243075651095No Hit
CACGAGGGGGAGATCGTGCTGTTCGCCTCAAGGCGGCAAGGTTACCTGTAGC1300.10748243075651095No Hit
GTGTTGGGGATAAAAATGTATGCATAAATGAGAATAGATAACACAAAATGAA1290.10665564282761472No Hit
AGGCCAGGAAGTTTAATCCTACCGTGTGCTCAGAAGGTGACGAGACAGAAGT1280.10582885489871849No Hit
TAAAGCGGGACGATGATGGAGCAACCAAACTCAATGATGAGGCACGGCGGCT1280.10582885489871849No Hit
TTTGGTGGGAGACGTCTGAATAATTCATTATTCGAGCCCAGCTCTAGTACAA1270.10500206696982224No Hit
CTTTGTGGGGGGGCACTGCAGGGAATGATGGGAAACTGTAAGTCTGCTCAGC1260.104175279040926No Hit
GAGAGGGGAAGTAGAACTCTTTTTCATATATTTACTATTCATTTGGAGTACT1260.104175279040926No Hit
AAGACAGGGACTTTGAGAAGGTCATGCATACAAGGAAGAGGCATATGGAACT1260.104175279040926No Hit
GTATGAGAGGGAATATAATGTTTGTTTCTGTCTCCTTGCTTTTCTGTACTGC1250.10334849111202976No Hit
GAAGCCGGGCTGAAATATTTCGTAAATAAACAGAACTTTTGTTATTGTAGGC1240.10252170318313353No Hit
GTTTAGGGAAACTCCTGACCTCAGGTGATCCACCCGCCTCAGCCTCCCAGAG1240.10252170318313353No Hit
AGTGATGGGATTGTCATGTCATTGGAATCATATAGGATGTTGTCTTTTCAGA1230.1016949152542373No Hit
ATGACGGGGCATTTTGCCAGGATTTTGTTGTGCTGAGATTGCCAATCACGGT1230.1016949152542373No Hit
GTGTAAGGGAAGTAACCACATCTCAGGCGGCAGTGAAGTCCAGATAGTTTTG1230.1016949152542373No Hit
CTATGATCGGGAAGCTAATGTAGACTCGCCTGGCCATTTCCTGGTTTCCTCC1230.1016949152542373No Hit
TATGTGGGTGCGAGGGGAGGAGCATTTTCAAAATTAGGCATCAAAATGCAAA1230.1016949152542373No Hit
TTGACTGGGATGAACGATATGGCTGTGAAGACAGAATGTGAACTAAAATTGA1220.10086812732534106No Hit
TTTGCAGAGGGATCATTATTTTTTGTGCACTTATGCGCACAGAAGATACTGT1220.10086812732534106No Hit
TCGATGGGGATTTCAATTGTGGAGCCTCATTTTAAGTGTTCATTCTTTTAAG1210.10004133939644483No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)