FastQCFastQC Report
Sat 31 Dec 2022
SRR5848850.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848850.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120153
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT6440.5359832879744991No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5480.45608515809010175No Hit
TTAAAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1860.15480262665101993No Hit
AACACAGGGGCCTTTGCTTGCTGTGTGGAAAAGGATTGGAGTGGGGCAAGAA1520.12650537231696254No Hit
TCAGCCGGGTATTGAAACATATTAAATGTATAAAACTCCATAAGTTTATATC1480.12317628357177932No Hit
TAAGCGGGGGGATATGAAAGGATGTGTCTTATTGTTTTATGCGTTAAGGATA1480.12317628357177932No Hit
ACTAAGGGTATAAGAGAAGAAATATTGGAATACTGCATTTGTGGTGGAAGGT1440.1198471948265961No Hit
ATTGCTGGGGCAAGCAAACAGGCGATTACAGTGCAGGGGACTCGCGCAGCAG1420.11818265045400447No Hit
TCGATGGGGATTTCAATTGTGGAGCCTCATTTTAAGTGTTCATTCTTTTAAG1400.11651810608141286No Hit
GCACTTGGGCTGCTGCCTGGGAGCTGAGGCACCCGTGCCAGTGGCAGAGGCC1390.11568583389511705No Hit
TTCGTTGGGGATCACTTGAGATCAGGACTTTAAGACCAGCCTCGGCAACAAC1370.11402128952252544No Hit
TTGACTGGGATGAACGATATGGCTGTGAAGACAGAATGTGAACTAAAATTGA1340.11152447296363802No Hit
GAGTCGGGACAATAATATTGGGAGACTTTTACACCCCACTGTCAATATTAGA1310.1090276564047506No Hit
TTTGCAGAGGGATCATTATTTTTTGTGCACTTATGCGCACAGAAGATACTGT1300.10819538421845482No Hit
CCGTATGGGATATGTCTTTGTATTACATCCAGCCAGCCTCAGAGGCTGGCCC1300.10819538421845482No Hit
AGTTTAGGGTCAGAATGACAGGGCAGTGGATTACTGTTTCTGGATCACCAGA1290.107363112032159No Hit
AGACGGGATTGAAGGGCTGTCCTTACACAGAGTTCCTTAGGAAAATCTGGGA1290.107363112032159No Hit
AGTGATGGGATTGTCATGTCATTGGAATCATATAGGATGTTGTCTTTTCAGA1280.10653083984586319No Hit
GTAGAGGGGGTATATATTCAGTAATGGAATTGCTGAGTCAAATGGCATTTCT1280.10653083984586319No Hit
GTAAAATGAGGACTGTAATTGTAATAACTTCATAGGGTTGTCACATGCATTT1280.10653083984586319No Hit
CATCTAGGGGAACGCGGGGCGAGCGGAGCCGGCTGAGCGGGCGCCGAGCTCC1280.10653083984586319No Hit
GATGAGTGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACA1280.10653083984586319No Hit
AGGCCAGGAAGTTTAATCCTACCGTGTGCTCAGAAGGTGACGAGACAGAAGT1270.1056985676595674No Hit
GGGCCTGGGATATAAATTTTATTTAATTTTTCTTTCTAACTAATCACAATGA1270.1056985676595674No Hit
CATAGGGAAGATCACCCGAGTGGAGATGCTGGAGATCATCGAGGCTATCTAC1270.1056985676595674No Hit
CAAGCCGGGAAATTTATGTAGTGCTGAACTTTAATATCTTATCTGATATGGC1260.10486629547327157No Hit
GAATTAGGGGAAATACATTATATTGTACGTTTGAGATAATTCTAGTACAAAG1260.10486629547327157No Hit
TGTCTAGGGATGTGAGACAAGACACTGTCTTTGCAAATGAGGAAAACAGACA1260.10486629547327157No Hit
CTACACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGG1250.10403402328697578No Hit
AAGAATGGGTGGCACAGCCTAGGGTTTCCTTTGAGGATGATAACCTTAGAAG1230.10236947891438415No Hit
TGCATGGGGACCCCAACATGGCTGCGCCCGTGGGACCGGTGAAGTTCTGGCG1230.10236947891438415No Hit
ATTCTAGGGAACCTCCCACCTAACTTAAGACTCATCTTGAAACCATCCCTAT1230.10236947891438415No Hit
AAAAACGGGCAACACCCTCATACCAAAGCCAGATAAGGACACTACAAGAAAA1220.10153720672808836No Hit
TGAAGTAGGGACGCTCTCCGATCTTCAATGGGACGGGGATGGTGGAAGAGGG1220.10153720672808836No Hit
GCGTAATGGGGTGAATAAAAATAATTTTAGAAAAGTTATCTATTTTTATTGT1220.10153720672808836No Hit
GCATGAGGGAACCTGTATTTATCACATTGTCAAACAGAATTTTTCTTTGAAT1210.10070493454179255No Hit
TTTTCAGGGCTTCACATAAAAAATAGGGTGTTAATACCACCTTTACTGTACT1210.10070493454179255No Hit
CTATGATCGGGAAGCTAATGTAGACTCGCCTGGCCATTTCCTGGTTTCCTCC1210.10070493454179255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)