FastQCFastQC Report
Sat 31 Dec 2022
SRR5848843.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848843.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58847
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT6901.1725321596682925No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5670.9635155572926402No Hit
GCATCCGGGTGTCTTTCTTAACTCACCCAACCTAGGCTGACCGAATGTAGTC1930.3279691403130151No Hit
GCAACTGGGCGTGGAGGCTGACTTTGCCGTCATTGCTGGTGTTTCTAACTGG1770.300779988784475No Hit
CTACTAGGGACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC1660.2820874471086037No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1600.2718915152854011No Hit
GCTGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1590.2701921933148674No Hit
GCAATGAGGGGTCATTTTTATTTTATTTAGTGAAAAGGATTTGGTGAAACAG1520.2582969395211311No Hit
CACATGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1430.24300304178632728No Hit
GACAATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1420.24130371981579352No Hit
TACGCAGGGCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAG1410.23960439784525972No Hit
ACGACGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1350.2294084660220572No Hit
CGGTTAGGGTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGA1350.2294084660220572No Hit
CAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCAT1300.2209118561693884No Hit
TAACCAGGGATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCA1300.2209118561693884No Hit
GGGACAGGGTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCT1280.2175132122283209No Hit
TTGCGCGGGATGGAAGATGTCTCCTAGAGACATCCTGGGGACATTGTAGCAG1250.21241524631671962No Hit
GCATGAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1230.20901660237565212No Hit
TGCACAGGGCGAACAGCACCCTGCATCTGGGGGATTGAAGCAGTCGCTGACC1230.20901660237565212No Hit
AGGTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1220.20731728040511835No Hit
CTTGCTGGGATTTCCACCAGTAGTGTATAAGCATTAAGCATTCTGTTTTCTC1220.20731728040511835No Hit
TCGCATGGGTATACAAAAGTGTTTTCCACTAATTTTGCATGCGTATTTATAA1210.2056179584345846No Hit
GGTAGTGGGAACCCAGAGAAAGACTTAGAGTAGACAGAATTCACTCAAATAA1200.20391863646405084No Hit
TGCTACGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC1200.20391863646405084No Hit
ATAATGGGGCGATCTCGCTCCGGGCATAGCACAATGTCTTTCTATAAGATAT1180.20051999252298333No Hit
ACCTAGGGCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA1170.19882067055244956No Hit
TGGCTTGGGCGAGACCGGCCTGGCCAACATGGTGAAACACTCTCTGCTAAAA1170.19882067055244956No Hit
TCTCAAGGGGGCTACTGCCGGTGCAGCCGCCAAACCGGTGCCTCGGTGACGA1160.19712134858191582No Hit
CATAAAGGGATCCCATTACTGGGTATATACCCAAAGGACTATAAATCATGCT1140.19372270464084831No Hit
CAGAGCGGTGTGTTTATAGTGGTACCTGACATGTAGAAAATGCTTAAGTTAT1120.1903240606997808No Hit
CTAATTGGGACTACAGAACCCTGAATATGCCAATGTGTATTTTATATCTCCA1110.188624738729247No Hit
TGGCCAGGGAACATGAGTGACATTTATTATAATTGTTAAGATAAGAGATAGT1090.1852260947881795No Hit
GATTAAGGGAAGGTTGAAAGACCTGACATCTCACACAATGGGGTTCTGGAAT1070.181827450847112No Hit
TGCTATGGGCAACTGACCGGATGAGGTCCACCCAAACTGTGGATGGTAAGTT1050.17842880690604448No Hit
CAGCACGGGCGATCTCTACACGGGACGCTCTTCCGATCTTACATGGGACTTT1050.17842880690604448No Hit
TCGTGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1050.17842880690604448No Hit
CAGGCAATGCCAAAACAAGGTTTCATGTTTTGAAAATGCTACTGAATTTGTC1050.17842880690604448No Hit
CAGTCGGGACTAAGAGATACATTGGAACACACTATTGACAAATCAAAATGGA1040.17672948493551072No Hit
AATTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1040.17672948493551072No Hit
GAACTATGGGTGGGCATGATTAAAACGAGGAAGTTTTACCTTTTCTATAATG1030.17503016296497698No Hit
CAAAGTGGGTGCTGCCACTGTAATAAAAGCTATTTTGGATTTGCCATGTCCA1020.1733308409944432No Hit
CACTTTGGGAAGCAATCCACTGGCCTCCGCCTTCCAAAGTGCTAGGATTATG1020.1733308409944432No Hit
TTTCATGGGAACATCAGGAATTGGCCAGGCGTGGTAGTGCACACCTGTAATC1010.17163151902390947No Hit
ATACCAGGGCCACGCTGCTGGAGCACCGGCACACACACATCAGCGAGAAGCC1010.17163151902390947No Hit
TTAGCCGGGCGATCTTTCGACGGGTAACAGCTAAGGACTGCAAAACCCCACT1000.1699321970533757No Hit
ATGCCAGGGCGATCTTTGGATAGGGACCATGTTAAGAATCAATCCTTCCCAA990.16823287508284196No Hit
AGCATAGGGCTTCTGTGAGTTATATAATGATAAATTGGCATAATTATCAATA990.16823287508284196No Hit
ACAGACGGGATGGAGGGGGGACCCGGGTTGAAAGAGGGAGTGAGAAAACCTT990.16823287508284196No Hit
CTCAGAAGGGGATCTTCACGTGGGGGAGCATTTTATGGCCCTAAAATTGACA980.1665335531123082No Hit
TATTGGGGGAAAGATGAGAAACTTGAGGCCCAGAGAGGTGAAATAATTTGCT970.16483423114177442No Hit
TAGTTAGGGAAGGGAAAGGTGTACATGAACGACCCCCAGGGCTTTAACAGGG960.16313490917124068No Hit
ACTGATGGACTGGGAGGTGCGCTACGAGCCGGACAGCAAGGCGTTCGGCGTG950.16143558720070691No Hit
TATATGGGGATGAGCCACCGTACCCGGCCTCTTAACTCATTTTCAGTGTTGG950.16143558720070691No Hit
TAGCCTGGGTTGGTGGTCGCAATATAAAGAAGCAGTGCTGCAAGATGAGAAA950.16143558720070691No Hit
AACCGGGATGTGCCTGTGTCAAAAGAAAAAAGAAGAAAAACTTCAAATAAAC940.15973626523017317No Hit
TGCTCAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT920.15633762128910567No Hit
AGTTTTGGGACATACATCAGAGGGCCCAGTTTCTTTTTCTTCCTTTTTAAAA910.1546382993185719No Hit
GGCCATGGGGAAATTCAAACGCTCATACTTTGCTAATAGGAACATAAAATGT900.15293897734803813No Hit
GCACTTGGGACCTTCACTACCTTGAAAAAGAGACCAAATTTCAGAAGGAAAG890.15123965537750436No Hit
AAGCGAGGGCTAACGATGGGTCTGTGAATGGGAGATCTAGAAGTCAAGGACA870.14784101143643685No Hit
TACGAACGGGATTCCTCTTGTGGAGGTCCTCATACCTGACATTTGCATACCA870.14784101143643685No Hit
CGCTGTGGGCCGAGGGGGTCCCCAAGAGATCCAGGCTGTCATGTGATTTATG850.14444236749536934No Hit
TCTGCTGGGTGAGTTTTCCAAGAAAACAACTCCTTTAAATTGTGAGAATAGG850.14444236749536934No Hit
CATGGTGGGATGAATTGAATTTGCTGCCAGGTGCTGCCAGACTGAATTTGGC840.14274304552483558No Hit
TCAAACGGGAAGTAGAAGTTCTAGATGACTGAGCAGCTGAGAAAGAGCAATT840.14274304552483558No Hit
TGCATAGGGGTATTAGAATATAATAAATCTCCAGTTATAGTCAATTACTACC830.14104372355430184No Hit
TCACACGGGTCTGAGTAGGGGCTGGAGTGCAGTGGCACAATCTTGGCTCCTT830.14104372355430184No Hit
TTGGTTGGGATGCTCATTATGACCTGGTTATAGCATTACTCCTCAAGGAAAT830.14104372355430184No Hit
GTATCGGGAATCAAACTAACCTCAAAACAAATGATAACCATACACAACACTA820.13934440158376807No Hit
TTTCAGGGGAGATGGGAGTTAGCTAAATCCACGTACAAGATTGTGTGGTTTA820.13934440158376807No Hit
CGGCCAGGGATTATGCACCCATTAAAATGTTTATATAGGCTGGGCTCACACC820.13934440158376807No Hit
AGCTACGGGAATGACAGGGCTTTAAAGATTTTTTAAAATAGGGAAATTAGGA810.13764507961323433No Hit
TACTAGGGGAGCTCTGAAAAGACAGCGAAAAAAAGAGCCTCTGATCTTGTCA810.13764507961323433No Hit
GCTACAGGGATGGTATTATCTCTTAAATGGCTACCTCAAAATTAAAAGGCAA800.13594575764270056No Hit
AACAACGGGTCAGTAATCACCACTAAAGAACTTATCCACGTTAACCAAACAC800.13594575764270056No Hit
TGTAACGGGATTTAAGAAAACCTTTTGTGTGTGTATTTTGCCATTTGTTACA780.13254711370163305No Hit
GCTAGCGGGTGAATTCTTTGATATAATGGACAGCCGCTGTACTGTGTTAAGG780.13254711370163305No Hit
GTTGCCGGGCTCTGGACTTTAAAACTCAGCATCTTCTGAACCAGAGGCATTT780.13254711370163305No Hit
CTGAATGGGCGATCACAACAGGGGCACATGGGAGGGAGGAGTGGGGCCACAG780.13254711370163305No Hit
ACGTTATGGGCTCTGAAATAGACAATAATAGTACCTACCTATCCCAGGATTT770.13084779173109928No Hit
TGATTTGGGATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCT770.13084779173109928No Hit
CGCAAAGGATGGTTCAGGTACTGTAAACTGTGGAGGAGTGACTAATAACCGT770.13084779173109928No Hit
GCTGTTGGGGTACTACATCTAAGAACTAGGCAATGATTTGAAAGCATTTTTG760.12914846976056554No Hit
CGTCAAGGGAGGGTCCTGCACGGACTTTAACGATGCAAAGAGGCATGAACAT760.12914846976056554No Hit
CATTGCGGGGCTCTTCCGATCTTTTAGCGGGCAAATTTCTAAACCTGCACCT760.12914846976056554No Hit
GGCTCTGGGCGATCTAAAGCTGGGATGATTAGCAAATATTTTCTTCCATTCT750.12744914779003177No Hit
GTTTCGGGGGGGTGTTTTTGTGAGGGTGATTTTGGATAAGATTAACATTTAA750.12744914779003177No Hit
GTATACGGGATATATTTTTAATGTTTAATATTTAGATGACAGTATGCTATAT750.12744914779003177No Hit
GAAACCGGGATGGATACTCTGTATGTCTAAACATTTTTGATAATATATAGTC750.12744914779003177No Hit
CAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAATCTCACTCAAAG740.12574982581949803No Hit
TTTATGGGGGCTCTCTTCCTCGGCGCTGCCTACGGAGGTGGCAGCCATCTCC740.12574982581949803No Hit
TAACAAGGGATGCCTTCTGATAACTTATGCCTGCCAGTGGAGCTATGGGAGG740.12574982581949803No Hit
TGTGAAGGGCAGTGCACCTAGTTTAAAAAGGAGTAAGTCATCAAGCATTTCA730.12405050384896427No Hit
TTACCGGGCAATAATGTTGTGAAGTAAAACAATCAGTACTGAGAAACCTGGT730.12405050384896427No Hit
GCTTTAGGGCTTTCTGATCCTTGGTGAAGTATGTTGGAAGTAGTATTCCAAG730.12405050384896427No Hit
TGCCCCGGGCCAGCAGCCCAGCCAGCAGCACAGTCTCTATGGTGCTGAGGAA730.12405050384896427No Hit
TCGCAAGGGCTGGATTAACGAATATTTAGATTGTTTCCAAATTTTTACTATT730.12405050384896427No Hit
GGTCTAGGGAGAGACACAGTCAGAAGCTCCGAGGCTACCGCGATGGTCCAGG720.12235118187843051No Hit
CGAAGCGGGAGTTCAGCCCATCCAGGATTCAGACGGGAAGAAGGTGGGGCTG720.12235118187843051No Hit
TAGTATGGGATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCG720.12235118187843051No Hit
TAACTAGGGATATTTGTGAATTAATGAATCAATAATTATATCAGATTAGCAT720.12235118187843051No Hit
GCTCTTGGGCATATTAGTTTATTGGGTTATTTCAGGGCTATTGACTTACGTA720.12235118187843051No Hit
GAGTCAGGGCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGG710.12065185990789676No Hit
GCACGCGGGATGAAAGTAATGATAATCAAAACCATCCATGTTGTAAGAATTG710.12065185990789676No Hit
AGCTTTGGGCTTAAATTGTTGGTAATTACATCTCATTCCTCCAAAATATTAT710.12065185990789676No Hit
TTTAATGGGCGATCTGTGAACGGGAGTTATTTTCTATTTAATATCAGTTCCT710.12065185990789676No Hit
GTAGGTGGGGAGTTCTGCTGTAGTTCCTTAATGTGTAACCAACATGCTTTCA710.12065185990789676No Hit
ACCAACGGGATTAAAGAGCCCTGTCCTGAAACTTCTACTTCCCCCTATTCTT710.12065185990789676No Hit
GGTCGGGTCAGGCAAGAAGGTCACAGGTTCCTGCTGTTTTTACCTGAATGTC710.12065185990789676No Hit
ATGTGGGGATTTCAGAGGAGTAAGGCATGCCAAGCAGCATGCTCAGTGGGGT710.12065185990789676No Hit
CACGCACGGGATGTTTTCTTCTAGTAGTTTCATAGTTTCAGTTCTGATGTTT700.11895253793736298No Hit
AGACACGGGATGTGGTATATTCCCTTTCACCCTCAGATGTGTGACATCTACC700.11895253793736298No Hit
GTAAATGGGATCAGGAGAAAGACAAAAAAAGTCCATTTTTGCCACCTGCATT700.11895253793736298No Hit
TCTGGTGGGTGGGAGGCCCAGGTGGGTGGATCATGAGGTTAGGAGTTTGAGA690.11725321596682922No Hit
TCGGCCGGATTGTACCTAGTGGTACCTAGTGACTTCCAACCAAAGCCTTTGA690.11725321596682922No Hit
TTGCTGTGGGGTATAAGATAACATTTAAAGCTGTGATATCAGATTGTATCAC690.11725321596682922No Hit
ATAATAGGGTTAGTCGCTCTGGGTCGCGAGGGCGGCTCCCAAGATTCCGCAA680.11555389399629547No Hit
CCTATAGAATGTATTGTTTGAAAGTGTAGTGATATATTTGTGTTTTTATTTC680.11555389399629547No Hit
GGATCAGGGCTTTTTTCTTGATTCAGTGTTCATTTGGAGTATTAGTCAGTTT680.11555389399629547No Hit
GATTAAGGGATGTGATTGTTTGTGTGTCCCCTTTCCTCTTCTTTGCTTAACA680.11555389399629547No Hit
CTAATTGGGAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCAC670.11385457202576171No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCTTATAC670.11385457202576171No Hit
TAGAGAGGGCAGTTTCGTAAGTGAAGGAGAAATAAACTCCTTTACAGACAAG670.11385457202576171No Hit
AAGTGAGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT670.11385457202576171No Hit
TTTCTCGGGATTGCTGGGTGGTATTCCATTGTATGAATAGACCCAATTTTAA670.11385457202576171No Hit
ATAAGGGGAACCTAGACTATCCAGAGACTTTGGCCCCTGCCGGGTAGACTCA660.11215525005522796No Hit
TATGTAGGGATCCAGCCTTTATCATATAAGCATTGACATTATCCAGGCCTAG660.11215525005522796No Hit
CACAGAGGGGTTCCTCAGGAGAATGGAATGGAAGACCCATCTATCTCTTTCT660.11215525005522796No Hit
CATGTAGGGCTAGACCTTTAGTGTGCCATGCAGTCACAGGTCTGAAAAGCAG650.1104559280846942No Hit
TTAAGTGGGAGGAGGCTGGGAGCAGTTCCAGGCCACAGTGAAGTGTGTTGCT650.1104559280846942No Hit
CGAACAGGGGAATGAATCTTGAAAACAGAGTTGGGAGATAAAAGCAAGCTGT650.1104559280846942No Hit
TAATAGGGGCAGTCTGGCATGAACCTCGAGTGGTCCCAGAAGTGCCTTCAGG650.1104559280846942No Hit
ACGCCAGGGCCTGTGTTCCAATAAAACTTTACTGAATAGGCAGTAGGCCATA650.1104559280846942No Hit
TATAGCGGGAGTTTAACTGGGTGTCCCGGCTGGCAAACCTCACGCAAGGCGA650.1104559280846942No Hit
GTAGGGGGGGACGCTCTTCCGATCTAGAGACGGGCATGTCTGCCCTATACCC650.1104559280846942No Hit
AAGCACGGTCAGTGTCTCTGATATTTTTCCTAATGATTTCCATCTCCTTCTA640.10875660611416045No Hit
AATGTCGGGATATACAAACATTAAATTCCAGAGGACCAAAGACTTACATGTC640.10875660611416045No Hit
TGGTTTAGAGTACAATTTTTAATACAATTTAGCAAGAAAGACACAAACTTAA630.1070572841436267No Hit
CAAAATGGGCAGCTGCCTCATTTAAATCCTGCAGTGTATGTTTATATTGGTC630.1070572841436267No Hit
TACGGTAGGGCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAA630.1070572841436267No Hit
ATCGTGGGGATTTCTCCCAGCTTGTGTTGATGCCGCGATTTTGACTGAGACT620.10535796217309293No Hit
TATTGCGGGCAGGATATAAATGAAAAATTTTACAAGGTGATAGATTTTTATT620.10535796217309293No Hit
AGAGAGGGGAAAGTATAGGCGATAGAAATTGAAACCTGGCGCAATAGATATA620.10535796217309293No Hit
TGTCGAGGGAGATGAATGGATAAACAAAATGTCTTCTATCCGTGCAAGGGAA620.10535796217309293No Hit
CTCAATGGGTCCCCTTCCACCCTCAGCTCCACCCTCAGCAGATGATAATATC620.10535796217309293No Hit
GTCTCGGGGATGGTAGAATTTTCTCTTCTTGTCAGACCTTAGAAGTACCTAC620.10535796217309293No Hit
GGTTCAGGGAACCACCCAACTATCTATAAACCTAGCCATGGCCATCCCCTTA620.10535796217309293No Hit
CTGCAAGGGACTTCTGCTGGAAACAGCTAACAGACAGGGTAATAATAGCCTA620.10535796217309293No Hit
CCATCAGGGGTTTTTTCCCTGATGTTGGGTAGATGAGTGCATATACATGCTA620.10535796217309293No Hit
TCTTTAGGGATAGTCGTGAATGAAATGATCCCAAGATGCAGAAGTTTAGTCA610.10365864020255917No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT610.10365864020255917No Hit
TAAATAGGGAGCTGCTCTGCATCCCCCATAATTCCCCAGCTGGAGCGCTTGG610.10365864020255917No Hit
TTTGGCGGGGGTTGCCAACTCGGAGAGACTGAGCTGGGCCACGCAAGATGGC610.10365864020255917No Hit
GTTTAGGGGCTGCCGGTTTAAATAGAGTCGAATTGAGGTTGTTTCTGGCAAG600.10195931823202542No Hit
TGATTTGGGACATCTAAACTGCAGAAGATGGAGGCCTACAACTTGCGCTTAG600.10195931823202542No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCT600.10195931823202542No Hit
GCAACTGGACCTTGAAAACATGATGCTAAGTGAAATAAACTAAACAGAAAAA600.10195931823202542No Hit
AATTTAGGGGGGAGGCTGCGTTGGTAAATAAGCCCAGAGAAGTAGGCTGGGG590.10025999626149167No Hit
GCGGAAGGGAAGTAAGCATTTTGTTTATTTTTGGTTCAATACTTTGGGTCCC590.10025999626149167No Hit
AAAACTGGGTATTTTATCTGCAGCTATTGTAAATGGGATTACTTTCTTGATT590.10025999626149167No Hit
GTGAAAGGGAATGTAGCTGGTGACTTTAAGTTGAAACCAGCGCTCATTGACA590.10025999626149167No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)