FastQCFastQC Report
Sat 31 Dec 2022
SRR5848842.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848842.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57419
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT7401.2887720092652257No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC5100.8882077361152232No Hit
GCATCCGGGTGTCTTTCTTAACTCACCCAACCTAGGCTGACCGAATGTAGTC1520.2647207370382626No Hit
AACCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAG1500.2612375694456539No Hit
GCAACTGGGCGTGGAGGCTGACTTTGCCGTCATTGCTGGTGTTTCTAACTGG1430.24904648287152337No Hit
GCAATGAGGGGTCATTTTTATTTTATTTAGTGAAAAGGATTTGGTGAAACAG1410.24556331527891465No Hit
GGTAGTGGGAACCCAGAGAAAGACTTAGAGTAGACAGAATTCACTCAAATAA1400.2438217314826103No Hit
TTGCGCGGGATGGAAGATGTCTCCTAGAGACATCCTGGGGACATTGTAGCAG1390.24208014768630592No Hit
ACGACGGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1360.23685539629739288No Hit
ATAATGGGGCGATCTCGCTCCGGGCATAGCACAATGTCTTTCTATAAGATAT1350.23511381250108848No Hit
GCATGAGGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCT1330.2316306449084798No Hit
TCGCATGGGTATACAAAAGTGTTTTCCACTAATTTTGCATGCGTATTTATAA1330.2316306449084798No Hit
CTACTAGGGACTACTAACAGACCGCAACCTCAACACCACCTTCTTCGACCCC1320.22988906111217539No Hit
GCTGCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1320.22988906111217539No Hit
CACATGGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAA1290.22466430972326235No Hit
AGGTATGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA1280.22292272592695797No Hit
CACTTTGGGAAGCAATCCACTGGCCTCCGCCTTCCAAAGTGCTAGGATTATG1280.22292272592695797No Hit
CGGTTAGGGTACCCCTCACAATCATGGCAAGCCAACGCCACTTATCCAGTGA1270.22118114213065362No Hit
CAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTTCACTTCCACTCCAT1270.22118114213065362No Hit
TAACCAGGGATCGCTATCCCCACCGGCGTCAAAGTATTTAGCTGACTCGCCA1270.22118114213065362No Hit
CAGAGCGGTGTGTTTATAGTGGTACCTGACATGTAGAAAATGCTTAAGTTAT1250.2176979745380449No Hit
CTAATTGGGACTACAGAACCCTGAATATGCCAATGTGTATTTTATATCTCCA1210.2107316393528275No Hit
TCGTGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1210.2107316393528275No Hit
TGCTACGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGGC1200.20899005555652309No Hit
TATATGGGGATGAGCCACCGTACCCGGCCTCTTAACTCATTTTCAGTGTTGG1180.2055068879639144No Hit
TTTCATGGGAACATCAGGAATTGGCCAGGCGTGGTAGTGCACACCTGTAATC1170.20376530416761005No Hit
CAGGCAATGCCAAAACAAGGTTTCATGTTTTGAAAATGCTACTGAATTTGTC1170.20376530416761005No Hit
AACCGGGATGTGCCTGTGTCAAAAGAAAAAAGAAGAAAAACTTCAAATAAAC1160.20202372037130567No Hit
GACAATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1160.20202372037130567No Hit
TAGTTAGGGAAGGGAAAGGTGTACATGAACGACCCCCAGGGCTTTAACAGGG1150.2002821365750013No Hit
GGGACAGGGTCTTCGTCTGATCCGTCCTAATCACAGCAGTCCTACTTCTCCT1140.19854055277869695No Hit
GAACTATGGGTGGGCATGATTAAAACGAGGAAGTTTTACCTTTTCTATAATG1130.19679896898239257No Hit
TCTCAAGGGGGCTACTGCCGGTGCAGCCGCCAAACCGGTGCCTCGGTGACGA1120.19505738518608823No Hit
GATTAAGGGAAGGTTGAAAGACCTGACATCTCACACAATGGGGTTCTGGAAT1120.19505738518608823No Hit
TACGCAGGGCACCTCCTATTCTTGCACGAAACGGGATCAAACAACCCCCTAG1120.19505738518608823No Hit
TACTAGGGGAGCTCTGAAAAGACAGCGAAAAAAAGAGCCTCTGATCTTGTCA1100.1915742175934795No Hit
TGCTATGGGCAACTGACCGGATGAGGTCCACCCAAACTGTGGATGGTAAGTT1090.18983263379717516No Hit
GGCTCTGGGCGATCTAAAGCTGGGATGATTAGCAAATATTTTCTTCCATTCT1070.18634946620456644No Hit
TGCCCCGGGCCAGCAGCCCAGCCAGCAGCACAGTCTCTATGGTGCTGAGGAA1070.18634946620456644No Hit
TGCTCAGGGACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT1040.18112471481565334No Hit
TGCACAGGGCGAACAGCACCCTGCATCTGGGGGATTGAAGCAGTCGCTGACC1040.18112471481565334No Hit
TTAGCCGGGCGATCTTTCGACGGGTAACAGCTAAGGACTGCAAAACCCCACT1030.179383131019349No Hit
AGTTTTGGGACATACATCAGAGGGCCCAGTTTCTTTTTCTTCCTTTTTAAAA1030.179383131019349No Hit
TAACAAGGGATGCCTTCTGATAACTTATGCCTGCCAGTGGAGCTATGGGAGG1020.17764154722304462No Hit
ATACCAGGGCCACGCTGCTGGAGCACCGGCACACACACATCAGCGAGAAGCC1020.17764154722304462No Hit
CATGGTGGGATGAATTGAATTTGCTGCCAGGTGCTGCCAGACTGAATTTGGC990.17241679583413155No Hit
GTATCGGGAATCAAACTAACCTCAAAACAAATGATAACCATACACAACACTA970.16893362824152283No Hit
CTGAATGGGCGATCACAACAGGGGCACATGGGAGGGAGGAGTGGGGCCACAG970.16893362824152283No Hit
GCTACAGGGATGGTATTATCTCTTAAATGGCTACCTCAAAATTAAAAGGCAA960.16719204444521849No Hit
TGGGCTGGGGAATAGAGGGCAGGCGTTCCGAGCCTCTTCCTGAGCCCCAGGT930.16196729305630542No Hit
CTTGCTGGGATTTCCACCAGTAGTGTATAAGCATTAAGCATTCTGTTTTCTC930.16196729305630542No Hit
AGCTACGGGAATGACAGGGCTTTAAAGATTTTTTAAAATAGGGAAATTAGGA910.1584841254636967No Hit
TATTGGGGGAAAGATGAGAAACTTGAGGCCCAGAGAGGTGAAATAATTTGCT910.1584841254636967No Hit
TCTGCTGGGTGAGTTTTCCAAGAAAACAACTCCTTTAAATTGTGAGAATAGG910.1584841254636967No Hit
TGCATAGGGGTATTAGAATATAATAAATCTCCAGTTATAGTCAATTACTACC900.15674254166739232No Hit
TCACACGGGTCTGAGTAGGGGCTGGAGTGCAGTGGCACAATCTTGGCTCCTT900.15674254166739232No Hit
GGTCTAGGGAGAGACACAGTCAGAAGCTCCGAGGCTACCGCGATGGTCCAGG890.15500095787108797No Hit
AATTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.15500095787108797No Hit
GCTAGCGGGTGAATTCTTTGATATAATGGACAGCCGCTGTACTGTGTTAAGG880.15325937407478363No Hit
ACTGATGGACTGGGAGGTGCGCTACGAGCCGGACAGCAAGGCGTTCGGCGTG870.15151779027847923No Hit
CAGCACGGGCGATCTCTACACGGGACGCTCTTCCGATCTTACATGGGACTTT870.15151779027847923No Hit
TGGCCAGGGAACATGAGTGACATTTATTATAATTGTTAAGATAAGAGATAGT870.15151779027847923No Hit
CAAAGTGGGTGCTGCCACTGTAATAAAAGCTATTTTGGATTTGCCATGTCCA860.14977620648217488No Hit
TGGCTTGGGCGAGACCGGCCTGGCCAACATGGTGAAACACTCTCTGCTAAAA860.14977620648217488No Hit
ATAAGGGGAACCTAGACTATCCAGAGACTTTGGCCCCTGCCGGGTAGACTCA850.14803462268587053No Hit
TGATTTGGGATGAGCCAGGTGGCCGTGGCTGGACTGCTCACTGTGCTTGTCT840.14629303888956616No Hit
ACAGACGGGATGGAGGGGGGACCCGGGTTGAAAGAGGGAGTGAGAAAACCTT840.14629303888956616No Hit
GCTTTAGGGCTTTCTGATCCTTGGTGAAGTATGTTGGAAGTAGTATTCCAAG830.1445514550932618No Hit
ATGCCAGGGCGATCTTTGGATAGGGACCATGTTAAGAATCAATCCTTCCCAA820.14280987129695744No Hit
TCGCAAGGGCTGGATTAACGAATATTTAGATTGTTTCCAAATTTTTACTATT820.14280987129695744No Hit
GTTGCCGGGCTCTGGACTTTAAAACTCAGCATCTTCTGAACCAGAGGCATTT810.1410682875006531No Hit
GCAACTGGACCTTGAAAACATGATGCTAAGTGAAATAAACTAAACAGAAAAA810.1410682875006531No Hit
AGCATAGGGCTTCTGTGAGTTATATAATGATAAATTGGCATAATTATCAATA810.1410682875006531No Hit
TAGCCTGGGTTGGTGGTCGCAATATAAAGAAGCAGTGCTGCAAGATGAGAAA810.1410682875006531No Hit
AAGCGAGGGCTAACGATGGGTCTGTGAATGGGAGATCTAGAAGTCAAGGACA800.13932670370434874No Hit
CGCTGTGGGCCGAGGGGGTCCCCAAGAGATCCAGGCTGTCATGTGATTTATG800.13932670370434874No Hit
GTAAATGGGATCAGGAGAAAGACAAAAAAAGTCCATTTTTGCCACCTGCATT800.13932670370434874No Hit
GCACGCGGGATGAAAGTAATGATAATCAAAACCATCCATGTTGTAAGAATTG790.13758511990804437No Hit
CACGCACGGGATGTTTTCTTCTAGTAGTTTCATAGTTTCAGTTCTGATGTTT790.13758511990804437No Hit
GCACTTGGGACCTTCACTACCTTGAAAAAGAGACCAAATTTCAGAAGGAAAG790.13758511990804437No Hit
ACCTAGGGCAGCTGTCTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGA780.13584353611174002No Hit
TGTGAAGGGCAGTGCACCTAGTTTAAAAAGGAGTAAGTCATCAAGCATTTCA770.13410195231543565No Hit
GTATACGGGATATATTTTTAATGTTTAATATTTAGATGACAGTATGCTATAT770.13410195231543565No Hit
GATTAAGGGATGTGATTGTTTGTGTGTCCCCTTTCCTCTTCTTTGCTTAACA770.13410195231543565No Hit
CTAATTGGGAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGCAC760.1323603685191313No Hit
CATAAAGGGATCCCATTACTGGGTATATACCCAAAGGACTATAAATCATGCT760.1323603685191313No Hit
CATTGCGGGGCTCTTCCGATCTTTTAGCGGGCAAATTTCTAAACCTGCACCT760.1323603685191313No Hit
CAGTCGGGACTAAGAGATACATTGGAACACACTATTGACAAATCAAAATGGA750.13061878472282695No Hit
GCTCTTGGGCATATTAGTTTATTGGGTTATTTCAGGGCTATTGACTTACGTA750.13061878472282695No Hit
TACGGTAGGGCGCTGTCACTTTCCTAGGACTTCTAACAGCCCTAGACCTCAA750.13061878472282695No Hit
TGTCGAGGGAGATGAATGGATAAACAAAATGTCTTCTATCCGTGCAAGGGAA740.12887720092652258No Hit
TAGTATGGGATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCG740.12887720092652258No Hit
GCTGTTGGGGTACTACATCTAAGAACTAGGCAATGATTTGAAAGCATTTTTG730.12713561713021823No Hit
AGCTTTGGGCTTAAATTGTTGGTAATTACATCTCATTCCTCCAAAATATTAT730.12713561713021823No Hit
AATGTCGGGATATACAAACATTAAATTCCAGAGGACCAAAGACTTACATGTC730.12713561713021823No Hit
CGAATAGGGGTATCAGTTATTGTTCCTGGACTGGTAGTATGGAAGCGATAAG730.12713561713021823No Hit
TATGTAGGGATCCAGCCTTTATCATATAAGCATTGACATTATCCAGGCCTAG720.12539403333391386No Hit
TACGAACGGGATTCCTCTTGTGGAGGTCCTCATACCTGACATTTGCATACCA720.12539403333391386No Hit
CTCCTCGGGACTACCAAGTATCAAGAACATTATTATAATTTTTAGCAAATAT720.12539403333391386No Hit
ATACTAGGGTGGAAACATGAAATTTAGCTCAGGCATCGGCTCCAAATTAAAT720.12539403333391386No Hit
CAAAATGGGCAGCTGCCTCATTTAAATCCTGCAGTGTATGTTTATATTGGTC720.12539403333391386No Hit
GGCCATGGGGAAATTCAAACGCTCATACTTTGCTAATAGGAACATAAAATGT720.12539403333391386No Hit
TCGGCCGGATTGTACCTAGTGGTACCTAGTGACTTCCAACCAAAGCCTTTGA720.12539403333391386No Hit
GGGCATGGGACACAAATTTGATCAAACAAGGGGGTCAGTGCACACATTTTGA720.12539403333391386No Hit
GAGTCAGGGCAGCTACTTGGGAGGCTGAGGCAGGAGAATGGCGTGAACCCGG700.12191086574130515No Hit
GTAGGTGGGGAGTTCTGCTGTAGTTCCTTAATGTGTAACCAACATGCTTTCA700.12191086574130515No Hit
TTTGGCGGGGGTTGCCAACTCGGAGAGACTGAGCTGGGCCACGCAAGATGGC700.12191086574130515No Hit
AATTTAGGGGGGAGGCTGCGTTGGTAAATAAGCCCAGAGAAGTAGGCTGGGG690.12016928194500079No Hit
CTCAGAAGGGGATCTTCACGTGGGGGAGCATTTTATGGCCCTAAAATTGACA690.12016928194500079No Hit
ACGCCAGGGCCTGTGTTCCAATAAAACTTTACTGAATAGGCAGTAGGCCATA690.12016928194500079No Hit
AAAACTGGGTATTTTATCTGCAGCTATTGTAAATGGGATTACTTTCTTGATT690.12016928194500079No Hit
CGCAAAGGATGGTTCAGGTACTGTAAACTGTGGAGGAGTGACTAATAACCGT690.12016928194500079No Hit
GGATCAGGGCTTTTTTCTTGATTCAGTGTTCATTTGGAGTATTAGTCAGTTT690.12016928194500079No Hit
CAGACCACAGTGCAATCAAACTAGAACTCAGGATTAAGAATCTCACTCAAAG680.11842769814869644No Hit
GTTTCGGGGGGGTGTTTTTGTGAGGGTGATTTTGGATAAGATTAACATTTAA680.11842769814869644No Hit
TTACCGGGCAATAATGTTGTGAAGTAAAACAATCAGTACTGAGAAACCTGGT680.11842769814869644No Hit
AACTTAGGGCTCGCCAAGGGATAACAAACTGTCTCTCAGACCACAGTGCAAT680.11842769814869644No Hit
AAGTGAGGGATGCCCCTCATTTACATAAATATTATACTAGCATTTACCATCT680.11842769814869644No Hit
TTCGCTGGGGCTGAGTATAAAAAATAATCCAGAACTAAAGTTGTTAGATAAA680.11842769814869644No Hit
CACCCTGGGGGGATCCTGGAACAAGGAAAGGACACTAGGTCAAAACTAAGGA670.11668611435239205No Hit
CGAACAGGGGAATGAATCTTGAAAACAGAGTTGGGAGATAAAAGCAAGCTGT670.11668611435239205No Hit
TTTCAGGGGAGATGGGAGTTAGCTAAATCCACGTACAAGATTGTGTGGTTTA670.11668611435239205No Hit
AAGCACGGTCAGTGTCTCTGATATTTTTCCTAATGATTTCCATCTCCTTCTA660.11494453055608769No Hit
ACCAACGGGATTAAAGAGCCCTGTCCTGAAACTTCTACTTCCCCCTATTCTT660.11494453055608769No Hit
CTTAGCGGGATTTTATGTATATTAAGATAAGTCTGTAAGATACATTCCTGGC660.11494453055608769No Hit
TCAAACGGGAAGTAGAAGTTCTAGATGACTGAGCAGCTGAGAAAGAGCAATT660.11494453055608769No Hit
CTGCAAGGGACTTCTGCTGGAAACAGCTAACAGACAGGGTAATAATAGCCTA660.11494453055608769No Hit
GTAGGGGGGGACGCTCTTCCGATCTAGAGACGGGCATGTCTGCCCTATACCC650.11320294675978335No Hit
TAACGGGCTCGTAATGCTCACACACATACTTACCTTCTATAGGAATTATGAA650.11320294675978335No Hit
CGGCCAGGGATTATGCACCCATTAAAATGTTTATATAGGCTGGGCTCACACC650.11320294675978335No Hit
AGAAAAGGGCTGAGCTGTGATTGATCATCATACATGCATTTTGAATAGGGAC640.11146136296347899No Hit
AACAACGGGTCAGTAATCACCACTAAAGAACTTATCCACGTTAACCAAACAC640.11146136296347899No Hit
AAGCGCGGGAACCAATTTGTTAAAATTCCAAAAAGTTAACTGTTCTCTTCAA630.10971977916717462No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCCTGTCTCT630.10971977916717462No Hit
ACTCTCGGGACGATTTTCAAATCCACACAAAAAAGCAAAGGGCAGTAGTAAA630.10971977916717462No Hit
ATCGTGGGGATTTCTCCCAGCTTGTGTTGATGCCGCGATTTTGACTGAGACT620.10797819537087026No Hit
GGGCCCGGGATGAGTACTGGAAGTGTTTAGATGAGAACTTAGAGGATGCTTC620.10797819537087026No Hit
TCTTTAGGGATAGTCGTGAATGAAATGATCCCAAGATGCAGAAGTTTAGTCA620.10797819537087026No Hit
ATGCAGGGGAGTGCACAAATGTTCCTGTTTCTCTCCATCCTTGCCAACACTT620.10797819537087026No Hit
CGTCAAGGGAGGGTCCTGCACGGACTTTAACGATGCAAAGAGGCATGAACAT620.10797819537087026No Hit
CAATCTGGGTCTGTAGCTGGGATGTCAAAAGACAAAATTACAACAAATGTAG620.10797819537087026No Hit
GTTTAGGGGCTGCCGGTTTAAATAGAGTCGAATTGAGGTTGTTTCTGGCAAG610.1062366115745659No Hit
TTTATAGGGATAAAATCAACTAATCAAAAATGATATAGACGACAGCACTGGC610.1062366115745659No Hit
GAAACCGGGATGGATACTCTGTATGTCTAAACATTTTTGATAATATATAGTC610.1062366115745659No Hit
TTTAATGGGCGATCTGTGAACGGGAGTTATTTTCTATTTAATATCAGTTCCT610.1062366115745659No Hit
CCTATAGAATGTATTGTTTGAAAGTGTAGTGATATATTTGTGTTTTTATTTC610.1062366115745659No Hit
ATCTGAAGACCTGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGT610.1062366115745659No Hit
GTCTCGGGGATGGTAGAATTTTCTCTTCTTGTCAGACCTTAGAAGTACCTAC600.10449502777826154No Hit
ATCGGTGCGGGAGGCAGAGGCAGTGAGAATGAATGATTGGAACTGAAATACT600.10449502777826154No Hit
GCCTAAGGGCTACACGATGCTCTTCCGATCTAGCGGGAATCTTGCAAAAATG600.10449502777826154Illumina Single End PCR Primer 1 (95% over 22bp)
GTTAGCGGGAATACCTGGGTGGCAAAATAATCTGTACAACAAACCCTCCCCC590.1027534439819572No Hit
CACAGAGGGGTTCCTCAGGAGAATGGAATGGAAGACCCATCTATCTCTTTCT590.1027534439819572No Hit
TATAGCGGGAGTTTAACTGGGTGTCCCGGCTGGCAAACCTCACGCAAGGCGA590.1027534439819572No Hit
TCTGGTGGGTGGGAGGCCCAGGTGGGTGGATCATGAGGTTAGGAGTTTGAGA580.10101186018565284No Hit
CATGTAGGGCTAGACCTTTAGTGTGCCATGCAGTCACAGGTCTGAAAAGCAG580.10101186018565284No Hit
GCTAAAGGGACTTGAGGTCAGGATTTCGAGACCAGCCCTGCTGTACAAAAAA580.10101186018565284No Hit
CAATCGGGGTAGCAGTTGAGTGATGCTGGTTAGCTATTAAGGTGGCCTGTTG580.10101186018565284No Hit
CGAAGCGGGAGTTCAGCCCATCCAGGATTCAGACGGGAAGAAGGTGGGGCTG580.10101186018565284No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)