FastQCFastQC Report
Sat 31 Dec 2022
SRR5848834.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5848834.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148354
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGCCGTCT8210.5534060423042183No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACCTCCAGGTATCTCGTATGC7580.5109400488021894No Hit
AAGCCGGGCTTCTACCTCAGCCTGCCAAGTAGCTGTCACTACAGGTACCAGC1900.1280720438950079No Hit
GCTTCAGGGACAATTCTGAATTTGCTTTTCTTGCCTCTCTTTAGTCACCTGT1860.1253757903393235No Hit
TTACATGGGCTGAGAGGTGTTTAGGCACAGCAGAGGGGACGCGGGGGGCAAG1760.11863515645011258No Hit
CTGCTGGGATTACAGGTGTGAGCCACTGCACCAGGCCTACATTACTTTTTTA1710.11526483950550709No Hit
GCCATAGGGAACAACCTTCATGGAATTTCTAGTGGAATTGAATTTATAGAAA1690.1139167127276649No Hit
CTGCCAGGGCATTCTGTCCAGTTTCCTAAGAGAGACAGCTTGGAGTATGCTT1670.1125685859498227No Hit
AAACTAGGGAGGAGGAGTGACGGGAACCGGGAGCCGGCTCTATGCGCCTGGA1660.11189452256090164No Hit
ATTCCAGGGGCATTAAGAGATATAAATGCAATAGTATTTAAAGTGTCAGGCA1650.11122045917198053No Hit
GAACGAGGGGAGGGTCTTGTCTGTTAGAAGGAAAACTAACAAACAGAAAGGA1650.11122045917198053No Hit
AGCAGCGGGAAGGTGGGCGAGGCCTAGGAAGAGGGTCATTCTTAAGCCACAC1630.10987233239413835No Hit
TGGGTTGGGCTTCTCGTTGGTCAGACCGATGTCCATTACATTCTCACACACA1630.10987233239413835No Hit
TAGCAAGGGGTGGCTGTGGCATCTCAGTCCTATTTTTGAGTTTTTTTGTGGG1630.10987233239413835No Hit
AACACGGGGAGAGAATGAGAAATATTCTGCTGAGTGCTCTTTGACATTGTGA1600.10785014222737506No Hit
ACTCTCGGGGATTTGTCATAGGCTTTCCCCTTTCTTTCTGTTCTAGCAGCAG1580.10650201544953287No Hit
AAAGTCGGGTACAAGGCCTCAGAATTATTTGCCCAAGTGTATTCTTTAAATA1580.10650201544953287No Hit
TTTTGAGGGCGAGGAAAAAATACGGAGGATAACCACTATTTTTGTGCAAATA1580.10650201544953287No Hit
GTAGTTGGGTGTTGCAGCTGTCCCAGCTTCCAGGTGGCCAGGTGGGATGAAT1560.10515388867169068No Hit
TAGCCAGGGATTGGTCATTGATATCAGTATTATTATTTTGCATCCTCTGCTA1550.10447982528276958No Hit
AGAAAAGGGAGGGAAGTAACAGCCATATACTTTTGGGGGGTATGTCTTTGAG1550.10447982528276958No Hit
TAACCGGGGCCATCCTCCCCAACTCAAACCAGGGAAAGTAAGCTTGCCTAGG1540.10380576189384849No Hit
GGGTATGGGATCTGACAATGGGTGGCTCCCTGGGCCTGTTCATCCATCCCGG1510.10178357172708523No Hit
TAGCAAGGGGTATTTATTTCAGGTAGAATTTTTTTCGTGTAGTTTTTTAATA1510.10178357172708523No Hit
TTGTTTGGGGATCTCGAATAGGGTGACAGCAGTTCTGTATGCTTTCTGTAAA1500.10110950833816412No Hit
GTTTCAGGGGATTCATCTGAGGAGTCAGTCCTCTCGAAAGCTGCCAAAATAA1490.10043544494924303No Hit
TCTTAAGGGTACAGAAGAATTGGAATGAAGACAGGATTTTTTTTGTCAAAGA1490.10043544494924303No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)